Advanced Pg Research Center
Advanced Pg Research Center
Gireesh Kumar R.,Telangana University |
Vijaya Gopal A.,Advanced Pg Research Center |
Subhash Reddy R.,Telangana University |
Durga Rani C.H.V.,Telangana University |
And 2 more authors.
Journal of Pure and Applied Microbiology | Year: 2016
Sixteen bacterial isolates were obtained from insecticide treated tomato, brinjal maize and ground nut rhizospheric soils by enriching Mineral Salt Medium broth with supplement of chlorpyrifos, Phorate source. These isolates were characterized on the basis of cell morphology, cultural and biochemical properties. Among the eight chlorpyrifos degrading bacterial isolates, CDB-1isolate utilized the more pesticide. Among the eight phorate degrading bacterial isolates, the Phorate degrading bacteria (PDB)-1 isolate utilized the pesticide (Phorate) effectively. Degrading efficiency of isolates was determined by measuring the chlorpyrifos, phorate residual concentrations at intervals using Gas chromatographic method. Among the molecular diversity of 16 organophosphorus pesticide degrading isolates viz., chlorpyrifos degrading isolates 8, phorate degrading isolates 8 were also studied by PCR-RAPD technique. CDB7 shows separate lane with 26 per cent similarity compared to other isolates. CDB5 isolate shows 29 percent similar when compared with other samples. CDB3 and CDB4 reveal same genetic relatedness according to dendrogram. CDB2 and CDB6 showed 54 percent similarity and CDB1 shows 41 percent similarity. CDB3 and CDB4 isolates are more or less similar in the genome and functional properties. Whereas, CDB7 isolate was highly diverse among other isolates.. PDB5 and PDB6 showed 55 per cent similar in coefficient scale. Whereas PDB2 and PDB3 revealed 75 per cent relatedness. The other isolates, PDB1, PDB7 and PDB4 shows 64-66 percent similar. The PDB8 was highly diverse from remaining other seven isolates. PDB2 and PDB3 showed similar genome and functional properties.
Kumar R.G.,Telangana University |
Kumar R.G.,Advanced Pg Research Center |
Gopal A.V.,Telangana University |
Gopal A.V.,Advanced Pg Research Center |
And 4 more authors.
Ecology, Environment and Conservation | Year: 2016
In the present study sixteen bacterial isolates were isolated from crops like tomato, brinjal maize and Groundnut by the enriched Minimum Salt Medium broth with supplement of chlorpyrifos and phorate source. These isolates were characterized morphologically, cultural and biochemically. Among the eight chlorpyrifos bacterial isolates, CDB-1 showed the high population count at different incubation periods (1st day to 5th day) compared to all CDB inoculates. Similarly eight chlorpyrifos bacterial isolates, PDB-1 showed the high population count at different incubation periods (1st day to 5∗ day) compared to all PDB isolates. Further these isolates tested for functional plant growth promoting properties like P-solubilization, Indole Acetic acid production, Ammonia production and biocontrol activity (HCN production and Siderophore). The results revealed that among eight CDB Isolates five isolates were able to solubilize phosphate on Pikovskaya's media containing Tri calcium phosphate in the range of 10 mm tol7 mm. Among eight PDB Isolates six isolates were able to solubilize phosphate on Pikovskaya's media containing Tri calcium phosphate in the range of 10 mm to 25 mm. Among sixteen isolates, CDB-1 is highly IAA producer (+++) followed by CDB-6, CDB-2, CDB-4, PDB-1 were moderate producers (++), CDB-7, PDB-2, PDB-4, PDB-5, PDB-8 were weak producers (+). Out of all sixteen isolates eleven isolates produced HCN. The isolates, CDB-1, PDB-1, isolates produced HCN moderately (++), PDB-2, PDB-4, PDB-3, PDB-6, PDB-8, CDB-2, CDB-5, CDB-6, CDB-7, were weak HCN producers (+). Copyright © EM International.