Pest Control and Ayurvedic Drug Research Laboratory

Vidisha, India

Pest Control and Ayurvedic Drug Research Laboratory

Vidisha, India
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Mala F.,Pest Control and Ayurvedic Drug Research Laboratory | Ahmad P.,Indian Institute of Integrative Medicine | Shrivastava P.N.,Pest Control and Ayurvedic Drug Research Laboratory
Biomedical and Pharmacology Journal | Year: 2011

In order to break the dormancy of the lavender seeds an efficient protocal was carried out under invitro laboratory conditions. The seeds where washed with 0.1% mercuric chloride for 5-7 minutes and then with 70% alcohol for 1 minute. Then seeds where thoroughly rinsed with double distilled water. After washing, the seeds were divided into petriplates, each petriplate receives at least a group of sixteen seeds on a moist paper and the seeds were separately subjected to various treatments. For each treatment two replicates were used. A set of control was kept to compare germination efficiency. After the above treatments the seeds were kept under investigation for 25 days and the seeds were treated with distilled water regularly. Seeds for each treatment were kept in the lab conditions (average temperature 15-20°C). Replicates were monitored and details were recorded.


Khalid S.,Pest Control and Ayurvedic Drug Research Laboratory | Alia A.,Rajeev Gandhi College | Shrivastava P.N.,Pest Control and Ayurvedic Drug Research Laboratory | Rather M.A.,Pest Control and Ayurvedic Drug Research Laboratory | Sheikh M.A.H.,Government Degree College
Asian Journal of Pharmaceutical and Clinical Research | Year: 2017

Objective: The main aim of this study was to determine the in vitro antioxidant activity of Iris kashmiriana ethanolic leaf extract and also total phenol and flavonoid content was evaluated. Methods: Total phenol content (TPC) was determined by Folin–Ciocalteu method, total flavonoid content (TFC) was estimated by aluminum trichloride spectrophotometer method. Furthermore, antioxidant activity was revealed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, hydrogen peroxide (H2O2) scavenging activity, and reducing power assay. Results: The ethanolic leaf extract of I. kashmiriana showed TPC of 13.25±0.57 µg/100 µg gallic acid equivalents and TFC of 33.61±3.37 µg/100 µg rutin equivalents. The DPPH assay revealed IC50 of 0.418 mg/ml and for H2O2 radical scavenging IC50 was 0.476 mg/ml for the plant extract while as reducing power assay revealed concentration-dependent absorption values which clearly determine the antioxidant property of plant. Conclusion: From the results, it is apparent that I. kashmiriana ethanolic leaf extract possessed potential antioxidant activity which can be used to cure wide range of diseases. © 2017 The Authors.


ul Hassan Lone I.,Indian Institute of Science | Kumar A.,Indian Institute of Science | Khan F.,P.A. College | Iqbal Dar A.,Pest Control and Ayurvedic Drug Research Laboratory
Journal of Chemical and Pharmaceutical Research | Year: 2012

Physico-chemical and microbiological parameters were analyzed in leachate affected groundwater samples obtained at different locations viz. Bhanpor District, Union Carbide Area, Shivnagar, Mohali village, Peoples Group Colony and Nishatpora. during December, 2008 to May 2009 in order to assess the impact of leachate percolation on groundwater quality in District, Bhopal (M.P). Different physico-chemical parameters (pH, Total Dissolved Solids, Electrical Conductivity, Dissolved Oxygen, Free Carbon dioxide, Alkalinity, Chloride, Total Hardness and Biological Oxygen Demand) and bacteriological parameters (Total Coli form, Fecal Coli forms and Fecal Streptococci) were analyzed using standard methodology in each of the collected samples. Total dissolved solids (TDS), electrical conductivity (EC), and Na+ exceeded the World Health Organization (WHO) tolerance levels for drinking water in 62.5%, 100%, and 37.5% of the groundwater samples respectively with pH and Fe exceeding WHO limits in 75% of the samples. Our results suggest the altered Physico-chemical and bacteriological parameters compared to the drinking water standards laid by Indian Council of Medical Research (ICMR) and World Health Organization (WHO). For example the electrical conductance was in the range of980 - 1490 simens. A good number of samples are also showing significant amount of organic matter that provides nutrition for the growth and multiplication of microorganisms, thus not fit for drinking purpose. Thus it was concluded that most of the water samples were non-portable for human beings due to high concentration of one or the other parameter and some remedial measures are suggested to reduce further groundwater contamination via Leachate percolation, hence the present study demands for proper management of wastes in Bhopal.


Shrivastava N.,Samrat Ashok Technological Institute | Datar M.,Samrat Ashok Technological Institute | Saxena R.C.,Pest Control and Ayurvedic Drug Research Laboratory
Biomedical and Pharmacology Journal | Year: 2010

Cassia fistula Linn is used extensively in various parts of the world against a wide range of ailments, the synergistic action of its metabolite production being most probably responsible for the plant's beneficial effects. Its fruitpulp is used as mild laxative, against fevers, arthritis, nervous system diseases, all kinds of bleeding, such as hematemesis or hemorrhages, as well as cardiac conditions and stomach problems such as acid reflux.It has been reported as antibacterial, antifungal, with wound healing property, hepatoprotective activity, antioxidant activity, anti-inflammatory activity, anti-tumor activity and as an bioabsorption material. An attempt has been made to highlight the medicinal fruit through the pharmacognostic studies. Priminary identification of phytoconstituents was performed.


Lone F.A.,Government Degree College | Lone S.,University of Pune | Aziz M.A.,k-Technology | Malla F.A.,Pest Control and Ayurvedic Drug Research Laboratory
International Journal of Pharma and Bio Sciences | Year: 2012

This paper reports an ethnobotanical study conducted during the year 2010 and 2011 in the tribal areas of District Kupwara. The study was aimed to document the traditional folklore knowledge of the local people about the use of different plants or their products. A large number of people belonging to various tribal groups or races were interviewed during field trips and asked questions regarding the traditional use of plants. The data collected reveals that about 48 plant species belonging to 30families find use in day to day life including medicinal, aromatic and cultural.


Shrivastava N.,Samrat Ashok Technological Institute | Datar M.,Samrat Ashok Technological Institute | Saxena R.C.,Pest Control and Ayurvedic Drug Research Laboratory
Biosciences Biotechnology Research Asia | Year: 2010

The plant Alstonia scholaris has been used in different system of traditional medication for the treatment of diseases and ailments of human beings. It is reported to contain various alkaloids, flavonoids and phenolic acids. It has been reported as antimicrobial, antiamoebic, antidiarrhoeal, antiplasmodial, hepatoprotective, immunomodulatory, anti-cancer, antiasthmatic, free radical scavenging, antioxidant, analgesic, anti-inflammatory, anti-ulcer, anti-fertility and wound healing activities. There are also reports available for the traditional use of this plant for its cardiotonic, anti-diabetic and anti-arthritic properties. Many isolated constituents from Alstonia scholaris lack the reports of pharmacological activities, which support its further pharmacological studies. It is considered as one of the best Indian medicinal Herb. An attempt has been made to highlight the medicinal bark through the pharmacognostic studies. Priminary identification of phytoconstituents was performed.


Patel S.,Vikram University | Jat S.S.,Pest Control and Ayurvedic Drug Research Laboratory | Meena P.K.,Pest Control and Ayurvedic Drug Research Laboratory | Patel R.,Pest Control and Ayurvedic Drug Research Laboratory | And 2 more authors.
Journal of Pure and Applied Microbiology | Year: 2010

The study showed the effect of Vitex negundo crude extracts on mast cells degranulation activity. It was noticed that 100mg/kg.dose of the herbal drug when given p.o. to the albino rats showed 72% mast cells stabilizing activity which was similar to the standard drug Prednisolone .Only 20% cells were found disrupted the detail phytochemistry of extract showing this activity is under the process.

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