Pepscan is a procedure for mapping and characterizing epitopes involving the synthesis of overlapping peptides and analysis of the peptides in enzyme-linked immunosorbent assays . The method is based on combinatorial chemistry and was pioneered by Mario Geysen and coworkers. Wikipedia.
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Advances in Immunology | Year: 2012
Although vaccines have proven life saving against a myriad of infectious diseases, various pathogens have remained refractory to prophylaxis of their host by active immunization. New insights in the three dimensional (3D) structure, domain organization and dynamics of viral and bacterial surface proteins can guide the design of effective vaccines in several ways. In this review we highlight recent developments in structure-based vaccine design that are aimed at stabilization of native conformations and focusing immune response to conserved epitopes. Detailed 3D structures of pathogen surface proteins provide knowledge on how to minimize complex antigens or how to redesign the surface of an immunogen in order to induce only relevant neutralizing antibodies against a broad range of serotypes. Structure - based vaccines with reduced complexity and broad efficacy could greatly enhance the number of people that might benefit from the therapies that are developed. © 2012 Elsevier Inc.
Pepscan | Date: 2014-03-07
The disclosure relates to the field of candidate drug testing and drug development. Described are methods for providing a compound composed of at least one molecule attached via at least two linkages to a molecular scaffold, the method comprising providing a scaffold comprising at least a first and a second reactive group; providing at least one molecule able to react with the at least first and second reactive group; and contacting the scaffold with at least one molecule to form at least two linkages between the scaffold and the molecule in a coupling reaction, wherein the formation of a linkage accelerates the formation of a consecutive linkage. The coupling reaction may be performed in solution, such as an aqueous solution. Furthermore, described is a method for selecting a candidate drug compound comprising providing a library of the compounds and determining the binding of a target molecule to the compounds.
Pepscan | Date: 2014-03-07
The disclosure relates to the field of candidate drug testing and drug development. A method is provided for providing a compound composed of at least one molecule attached via at least two linkages to a molecular scaffold, the method comprising providing a scaffold comprising at least a first and a second reactive group; providing at least one molecule capable of reacting with the at least first and second reactive group; contacting the scaffold with at least one molecule to form at least two linkages between the scaffold and the at least one molecule in a coupling reaction, wherein the formation of a linkage accelerates the formation of a consecutive linkage, preferably wherein the coupling reaction is performed in solution, more preferably in an aqueous solution. Furthermore, a method is provided for selecting a candidate drug compound comprising providing a library of compounds hereof and determining the binding of a target molecule to the compounds.
Agency: Cordis | Branch: FP7 | Program: CP-FP | Phase: NMP-2007-1.1-1 | Award Amount: 4.94M | Year: 2008
The cause of diseases is often unknown, but their origin can frequently be found at the biomolecular and cellular level situated on nm-scale. Early diagnostics combined with early intervention on that nanoscale is one of the holy grail of modern medicine. Inorganic nanoparticles are very promising agents in that respect. One of the promising biomedical applications of these nanoparticles is their use as agents for tumor hyperthermia. Hyperthermia is a form of cancer treatment that uses an elevated temperature to kill the tumor tissue. Compared to the more conventional surgical procedures, it is hailed as a less invasive approach that could be used for small, non-defined tumors. Well-designed instrumentation in combination with engineered inorganic nanoparticles that (a) possess the desired physical properties to generate a local heat and that (b) can specifically target the tumor offer immense potentials for targeted hyperthermia therapy. The overall objective of the present multi-disciplinary project is to develop and to explore various metal/magnetic nanoparticles as agents for targeted tumor therapy. To strive for this overall objective, a successful integration and convergence of different technologies at the nanoscale is indispensable. In this project, we will focus on the synthesis routes of tailor designed biofunctionalized nanoparticles for hyperthermia. This requires a profound physical and chemical characterization of the synthesized nanostructures, but the project is certainly not limited hereto. It will also include a toxicological and biological evaluation of the different nanoparticles. Hereby a detailed exploration and characterization of the interaction mechanism of the biological entities and the nanostructures will be pursued to obtain a better understanding of the phenomena occurring at the nanoscale. In addition, this project also comprises the design of advanced instrumentation that can be used for a controlled hyperthermia treatment.
Agency: Cordis | Branch: H2020 | Program: RIA | Phase: PHC-03-2015 | Award Amount: 6.00M | Year: 2016
We hypothesize that inappropriate thyroid hormone action in target cells is a common mechanism underlying susceptibility to age-related degenerative diseases and co-morbidities. Although regulation of systemic thyroid status is well understood and underpins treatment of common thyroid disease, it is only in the last decade that the importance of local regulation of thyroid hormone action in tissue development, homeostasis and repair has been identified. During evolution, this complex temporal and cell-specific regulation has been optimized for development and reproductive fitness but NOT for ageing. Humans with their exceptional longevity are thus exposed to a prolonged period of suboptimal local thyroid hormone action. Consistent with this, thyroid status is a continuous variable within the population that is related to fracture risk, muscle mass and cognitive decline. Moreover, in healthy longevity thyroid status is characterized by thyroid stimulating hormone in the upper half of the reference range. In these studies, we will determine how local regulation of thyroid hormone action controls tissue homeostasis and repair, whilst its dysregulation is a common mechanism underlying chronic disease development during ageing. We focus on osteoporosis, osteoarthritis, neurodegeneration and sarcopenia as paradigm age-related, degenerative disorders. Using cutting-edge technology, we will (i) identify thyroid hormone dependent biomarkers for disease susceptibility in bone, cartilage, central nervous system and skeletal muscle, (ii) manipulate cell-specific thyroid hormone action in these tissues and (iii) develop cell-type specific modulators of thyroid hormone action. THYRAGE integrates cross-disciplinary expertise from clinical and basic scientists, endocrinologists, neuroscientists, gerontologists, and industry-based peptide scientists. These studies will identify and validate novel strategies for prevention and treatment of chronic age-related degenerative disease.