PEPRIC NV

Leuven, Belgium

PEPRIC NV

Leuven, Belgium

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Edge D.,Trinity College Dublin | Shortt C.M.,University College Cork | Gobbo O.L.,Trinity Biomedical science Institute | Teughels S.,PEPRIC nv | And 5 more authors.
Clinical and Experimental Pharmacology and Physiology | Year: 2016

Summary: Manufactured nanomaterials have a variety of medical applications, including diagnosis and targeted treatment of cancer. A series of experiments were conducted to determine the pharmacokinetic, biodistribution and biocompatibility of two novel magnetic nanoparticles (MNPs) in the anaesthetized pig. Dimercaptosuccinic acid (DMSA) coated superparamagnetic iron oxide nanoparticles (MF66-labelled 12 nm, core nominal diameter and OD15 15 nm); at 0.5, or 2.0 mg/kg) were injected intravenously. Particles induced a dose-dependent decrease in blood pressure following administration which recovered to control levels several minutes after injection. Blood samples were collected for a 5-h period and stored for determination of particle concentration using particle electron paramagnetic resonance (pEPR). Organs were harvested post-mortem for magnetic resonance imaging (MRI at 1.5 T field strength) and histology. OD15 (2.0 mg/kg) MNP had a plasma half-life of approximately 15 min. Both doses of the MF66 (0.5 and 2.0 mg/kg) MNP were below detection limits. MNP accumulation was observed primarily in the liver and spleen with MRI scans which was confirmed by histology. MRI also showed that both MNPs were present in the lungs. The results show that further modifications may be required to improve the biocompatibility of these particles for use as diagnostic and therapeutic agents. © 2016 John Wiley & Sons Australia, Ltd.


Gobbo O.L.,Trinity College Dublin | Wetterling F.,Trinity College Dublin | Vaes P.,PEPRIC NV | Teughels S.,PEPRIC NV | And 5 more authors.
Nanomedicine | Year: 2015

Aim: Superparamagnetic iron oxide nanoparticles (SPIONs) may play an important role in nanomedicine by serving as drug carriers and imaging agents. In this study, we present the biodistribution and pharmacokinetic properties of SPIONs using a new detection method, particle electron paramagnetic resonance (pEPR). Materials & methods: The pEPR technique is based on a low-field and low-frequency electron paramagnetic resonance. pEPR was compared with inductively coupled plasma mass spectrometry and MRI, in in vitro and in vivo. Results: The pEPR, inductively coupled plasma mass spectrometry and MRI results showed a good correlation between the techniques. Conclusion: The results indicate that pEPR can be used to detect SPIONs in both preclinical and clinical studies. © 2015 Future Medicine Ltd.


Grant
Agency: European Commission | Branch: FP7 | Program: CP-IP | Phase: NMP-2010-4.0-1 | Award Amount: 13.15M | Year: 2011

The aim of the MultiFun consortium is to develop and validate a novel and minimally-invasive nanotechnology system to improve cancer diagnosis and treatment. MultiFun nanotechnology is based on multifunctionalised magnetic nanoparticles to selectively target and eliminate breast and pancreatic cancer (stem) cells. The improved magnetic features of the MultiFun magnetic nanoparticles will lead to potential medical applications such as contrast agents and magnetic heating inductors. Moreover, magnetic nanoparticles can be functionalised with ligands to increase their affinity towards cancer cells in order to facilitate diagnosis of tumours by MRI. Targeting peptides and antibodies will be employed, including antibodies against cancer stem cells leading to early cancer detection by MRI means. The same nanoparticles will be used simultaneously as functional nanocarriers and heating inductors in order to provide a combined therapeutic modality. The synergistic effects of drugs, peptides, small RNAs and heat will be evaluated to determine the effectiveness of different therapeutic combinations. Interestingly, the use of ligands will favour the specific application of the therapeutic modalities to cancer (stem) cells, increasing the effectiveness and reducing side effects. Thus, MultiFun multimodal therapeutic approach is designed to efficiently remove cancer cells, including cancer stem cells, from the tumour site. The toxicity of functionalised magnetic nanoparticles will be assessed in vitro and in vivo to warrant a safe use and shed some light on the risks. The distribution and activity evaluation of functionalised nanoparticles will be performed in human breast and pancreatic cancer xenograft models. The use of novel magnetic nanoparticles for biomedical applications provides opportunities for new instrumentation: 1) detection and quantification of magnetic nanoparticles in blood, urine and tissues, and 2) magnetic heating induction for raising cell temperature.


PubMed | University College Cork, PEPRIC NV and Trinity College Dublin
Type: Journal Article | Journal: Nanomedicine (London, England) | Year: 2015

Superparamagnetic iron oxide nanoparticles (SPIONs) may play an important role in nanomedicine by serving as drug carriers and imaging agents. In this study, we present the biodistribution and pharmacokinetic properties of SPIONs using a new detection method, particle electron paramagnetic resonance (pEPR).The pEPR technique is based on a low-field and low-frequency electron paramagnetic resonance. pEPR was compared with inductively coupled plasma mass spectrometry and MRI, in in vitro and in vivo.The pEPR, inductively coupled plasma mass spectrometry and MRI results showed a good correlation between the techniques.The results indicate that pEPR can be used to detect SPIONs in both preclinical and clinical studies.


PubMed | University College Cork, PEPRIC nv, Trinity College Dublin and Mercy University Hospital
Type: Journal Article | Journal: Clinical and experimental pharmacology & physiology | Year: 2016

Manufactured nanomaterials have a variety of medical applications, including diagnosis and targeted treatment of cancer. A series of experiments were conducted to determine the pharmacokinetic, biodistribution and biocompatibility of two novel magnetic nanoparticles (MNPs) in the anaesthetized pig. Dimercaptosuccinic acid (DMSA) coated superparamagnetic iron oxide nanoparticles (MF66-labelled 12nm, core nominal diameter and OD15 15nm); at 0.5, or 2.0mg/kg) were injected intravenously. Particles induced a dose-dependent decrease in blood pressure following administration which recovered to control levels several minutes after injection. Blood samples were collected for a 5-h period and stored for determination of particle concentration using particle electron paramagnetic resonance (pEPR). Organs were harvested post-mortem for magnetic resonance imaging (MRI at 1.5 T field strength) and histology. OD15 (2.0mg/kg) MNP had a plasma half-life of approximately 15min. Both doses of the MF66 (0.5 and 2.0mg/kg) MNP were below detection limits. MNP accumulation was observed primarily in the liver and spleen with MRI scans which was confirmed by histology. MRI also showed that both MNPs were present in the lungs. The results show that further modifications may be required to improve the biocompatibility of these particles for use as diagnostic and therapeutic agents.


Coene A.,Ghent University | Crevecoeur G.,Ghent University | Dupre L.,Ghent University | Vaes P.,PEPRIC NV
Journal of Physics D: Applied Physics | Year: 2013

In recent years, magnetic nanoparticles (MNPs) have gained increased attention due to their superparamagnetic properties. These properties allow the development of innovative biomedical applications such as targeted drug delivery and tumour heating. However, these modalities lack effective operation arising from the inaccurate quantification of the spatial MNP distribution. This paper proposes an approach for assessing the one-dimensional (1D) MNP distribution using electron paramagnetic resonance (EPR). EPR is able to accurately determine the MNP concentration in a single volume but not the MNP distribution throughout this volume. A new approach that exploits the solution of inverse problems for the correct interpretation of the measured EPR signals, is investigated. We achieve reconstruction of the 1D distribution of MNPs using EPR. Furthermore, the impact of temperature control on the reconstructed distributions is analysed by comparing two EPR setups where the latter setup is temperature controlled. Reconstruction quality for the temperature-controlled setup increases with an average of 5% and with a maximum increase of 13% for distributions with relatively lower iron concentrations and higher resolutions. However, these measurements are only a validation of our new method and form no hard limits. © 2013 IOP Publishing Ltd.


Li X.,Ghent University | Torfs G.,Ghent University | Teughels S.,Pepric NV | Bauwelinck J.,Ghent University
Electronics Letters | Year: 2015

The first demonstration of a pulsed electron paramagnetic resonance (EPR) detection system based on the recently announced particle EPR (pEPR) technology for superparamagnetic iron oxide nanoparticles (SPIONs) applications is presented. The SPIONs have large magnetisation, which allows one to detect the electron resonance under a low magnetic field and at room temperature. However, the broad linewidth of the superparamagnetic particles leads to a short relaxation time which requires a quick damping of the transmit signal. The presented experiments on a commercial SPION prove the feasibility to detect a pulsed electron response from broad linewidth particles using the pulsed pEPR technology. © The Institution of Engineering and Technology 2015.


Li X.,INTEC Design | Torfs G.,INTEC Design | Vandewege J.,INTEC Design | Bauwelinck J.,INTEC Design | Verbiest J.R.,Pepric NV
Electronics Letters | Year: 2013

A new system for the quantification of super paramagnetic iron oxide nanoparticles (SPIONs) is presented. The proposed system relies on the particle electron paramagnetic resonance (pEPR) technique and utilises the linear response of the SPIONs to generate a pEPR signal under a static field. By extracting the pEPR signals, whose intensity is proportional to the SPIONs concentration, the quantitative information of the SPIONs is derived. To evaluate the system performance, sensitivity measurements have been conducted with two commercial SPIONs, FeraSpin M and Resovist, and reveal a measurement sensitivity of 300 ng. © The Institution of Engineering and Technology 2013.


Patent
Pepric Nv | Date: 2012-03-21

A system and method involve performing electron paramagnetic resonance on an object under study. The system comprises a first field generator adapted for generating an orienting magnetic field for orienting the magnetization of the object under study and a second field generator adapted for generating RF excitation waves at a frequency to generate electron paramagnetic resonance (EPR) in the object under test. The system also comprises a detection unit adapted for detecting the EPR signals emitted by the object under test and a control unit adapted for controlling the relative orientation of the orienting magnetic field induced by the first field generator with respect to the detection unit. The system furthermore comprises a processing unit programmed for combining detected EPR signals obtained using different relative orientations of the orienting magnetic field with respect to the detection unit.


A system for determining a reconstruction of a particle distribution in an object based on electron paramagnetic resonance (EPR) measurement data of the object comprising the distribution of particles is described. The system comprises a data input for obtaining electron paramagnetic resonance measurement data of the object under study. The system also comprises a processor for processing the obtained data by applying a numerical model for solving a numerical inverse problem of deriving from the electron paramagnetic resonance measurement data a reconstruction of the particle distribution. The system furthermore comprises an output port for outputting data based on the derived reconstruction of the particle distribution.

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