Schmidt R.,German Cancer Research Center |
Jacak J.,Johannes Kepler University |
Schirwitz C.,German Cancer Research Center |
Stadler V.,PEPperPRINT |
And 5 more authors.
Journal of Proteome Research
Based on a single-molecule sensitive fluorescence-linked immunosorbent assay, an analytical platform for the detection of lipoarabinomannan (LAM), a lipopolysaccharide marker of tuberculosis, was established that is about 3 orders of magnitude more sensitive than comparable current ELISA assays. No amplification step was required. Also, no particular sample preparation had to be done. Since individual binding events are detected, true quantification was possible simply by counting individual signals. Utilizing a total internal reflection configuration, unprocessed biological samples (human urine and plasma) to which LAM was added could be analyzed without the requirement of sample purification or washing steps during analysis. Samples containing about 600 antigen molecules per microliter produced a distinct signal. The methodology developed can be employed for any set of target molecules for which appropriate antibodies exist. © 2011 American Chemical Society. Source
Microarrays promise to advance biology through the parallel screening for many different binding partners. In the field of DNA microarrays lithographic techniques enables the combinatorial synthesis in densities of several ten thousand oligonucleotides per cm2. The availability of such high dense screening tools revolutionized the field of genomics. A similar development is expected for the field of proteomics, but was hindered in past times by low densities and the lack of an adequate diversity in peptide array production. This article describes recent developments in this field with an emphasis on methods that have the potency to overcome the obstacles in peptide synthesis and lead to affordable very high density peptide arrays. Source
PEPperPRINT | Entity website
PEPperCHIP Human Epitome Microarray For the very first time, the new PEPperCHIP Human Epitome Microarray covers all linear human B-cell epitopes of the Immune Epitope Database. The 28,895 different peptides of the PEPperCHIP Human Epitome Microarray are based on23,163 database epitopes of 2,542 different proteins and 468 different organisms ...
PEPperPRINT | Entity website
PEPperCHIP Custom Peptide Microarrays Specifications From single antigens translated into overlapping peptides to whole virus proteomes PEPperCHIP Custom Peptide Microarrays can be fully tailored in a uniquely cost-effective manner. Peptide Synthesis: On-chip using Fmoc chemistry and rountine double coupling steps Microarray Format: Standard slide format (3'' x 1'', 75 ...
Agency: Cordis | Branch: FP7 | Program: CP | Phase: ENERGY.2010.10.2-1 | Award Amount: 3.64M | Year: 2011
In a solar cell that converts energy as efficient as the natural plant photosystems, electrons should travel over very short distances, through an extremely ordered structure free of traps. Guided by these principles that were worked out at WIS and CUNY, we want to synthesize many different peptides, and consecutively screen variants thereof for a putative diode function. When linked to light-harvesting building blocks, these should yield a novel type of solar cell that is based on biomimetic principles. To achieve these goals, UPC will chemically synthesize a panel of artificial building blocks that are designed to harvest light and/or tunnel electrons. When assembled into many different peptides, some of these constructs are expected to function as a diode, and, when linked to light-harvesting molecules, as a solar cell. Peptides will be embedded into a self-assembled monolayer of alkanethiols on individual gold pads of a computer chip, which will be designed and manufactured by IMS. Each individual peptide can be addressed by feeding in, and at the same time measuring, the amount of current flow in both directions through the gold pads of the individual pixels. A similar kind of screen should then detect a functional peptide-based solar cell. 10.000 Peptides per cm(\2) will be synthesized by a particle based combinatorial synthesis recently developed by KIT-G and PPP. These peptides will be transferred in the array format to the chip where they couple to the gold pads. Therein, peptides will be coupled through cysteine residues to the flat gold surface, where they are embedded into a membrane-like structure. We expect that our evolution inspired approach may open a novel route to very efficient, and very cheap solar cells. This is due to the small percolation distances, highly ordered modular peptide structures, the large number of generated peptides, the ability to easily combine and modify eventually found peptide-diodes, and the frugal material consumption.