Yang Q.-P.,PeopleSHospital of Changshou District |
Zhou Z.,PeopleSHospital of Changshou District |
Lv B.,PeopleSHospital of Changshou District |
Tao Y.,PeopleSHospital of Changshou District |
And 3 more authors.
Chinese Journal of Biologicals | Year: 2012
Objective: To observe the inhibitory effect of combined application of vincristin (VCR) and endostar (Endo) on growth of subcutaneously transplanted rhabdomyosarcoma (RMS) in nude BALB / c mice and investigate the relevant mechanism. Methods: Nude mouse model of subcutaneously transplanted RMS was established by s.c. injection with the suspension of RMS PLA-802 cells into the right armpits of nude BALB / c mice. The model nude mice were randomly divided into one control and three test groups on day 8 after transplantation of RMS. The nude mice in three test control groups were injected i.p. with VCR, Endo and VCR + Endo respectively, each at a dosage of 0.2 ml, once a day for 2 weeks, while those in control group with physiological saline. The nude mice were weighed every 3 d, of which the tumors were measured for longitudinal and transverse diameters, based on which the sizes of tumors were calculated, and the tumor growth curve was plotted. The nude mice were killed 2 weeks after the first injection, of which the tumors were weighed, and the tumor inhibition rates were calculated. The transcription level of vascular endothelial growth factor (VEGF) mRNA in transplanted tumor cells was determined by RT-PCR, while the expression level of VEGF by Western blot. The distributions and expressions of VEGF and CD31 were determined by immunohistochemical assay. Results: Nude mouse model of EMS was copied successfully on day 8 after inoculation with PLA-802 cells, with a tumor formation rate of 84% (42/50). The bodyweights of nude mice in VCR + Endo group were significantly lower, while the sizes of transplanted tumors were significantly smaller, than those in control, VCR and Endo groups (P < 0.01). The tumor inhibition rates in VCR, Endo and VCR + Endo groups were 31.41%, 20.75,% and 48.41% respectively. Compared with those in control group, both the transcription levels of VEGF mRNA and expression levels of VEGF in VCR, Endo and VCR + Endo groups decreased significantly, of which those in VCR + Endo group were the lowest (P < 0.05). Immunohistochemical assay showed that the expression levels of VEGF and CD31 were significantly lower in VCR + Endo group than in the other three groups. Conclusion: The VCR and Endo at small dosages significantly inhibit the growth of transplanted RMS, while combined application of the two drugs enhanced the inhibition by a possible mechanism of inhibiting the angiogenesis of tumor.