Jain G.,Bhagwant University |
Jain N.,Peoples University |
Argal A.,Bhagwant University
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2014
Objective: Wheatgrass is known to have several medicinal properties to treat various disorders. Triticum aestivum Linn. is a traditional medicinal plant apparently used for bowel disorders, skin inflammation, infection, mouth sores and wound healing. It is also used for conditions as varied as headache to a folk remedy for diabetes. No data was found for the simultaneous use of it for wound healing and diabetes. The present study was designed to study the wound healing activity with the complications of diabetes. The objective is to help those people suffering from diabetes and not getting relief from pain and wounds. Methods: The 5% methanolic extract ointment of Triticum aestivum young leaves were evaluated for its wound healing activity in alloxan induced diabetic rats using excision wound model. The percent wound closure and their epitelialisation periods were observed for 16 days. Result: The wound treated with Triticum aestivum grass extract was healed in very efficient manner and the healing was very close to the standard intadine. Conclusion: The Triticum aestivum young leaves extract has shown similar wound healing property as compared with intadine. The quercetine and other grass factors present in the extract may be responsible for promoting the wound healing activity. © 2014, IJPPS. All rights reserved.
Shukla T.,Peoples University |
Pandey S.P.,Peoples University |
Patil U.K.,Dr Hari Singh Gour University
Indian Journal of Pharmaceutical Education and Research | Year: 2014
Indomethacin (NASID) is used as potent anti-inflammatory drug with prompt antipyretic action, mainly used for the treatment of osteoarthritis with half-life of 4.5 hours. The basic objective of present work was to prepare floating delivery system of Indomethacin for once a day formulation using gas formation technique for prolonging the gastric residence time, so that the dosage regimen and gastric irritancy can be reduced. Indomethacin was estimated in the formulation by using UV/Visible spectrophotometer (Shimazdu UV-1700) at 321 nm. Tablets were evaluated for various parameters and it was observed that the formulation having 75 mg of Indomethacin, 34% HPMC K4M, 12% sodium bi-carbonate and 20% of lactose shows better result having 96.37% drug release within 24 hr with floating lag time of 155 seconds and floating time of 22 hr. It was also observed that as the concentration of gas generating and channeling agent increases, the chances of burst release and tablet erosion increases simultaneously. In the present study it was concluded that the floating drug delivery system with reduced floating lag time and sustained drug release of Indomethacin was obtained and could be a promising gastro-retentive drug delivery system.
Hongal S.,Peoples University |
Torwane N.A.,Peoples University |
Goel P.,Peoples University |
Chandrashekar B.,Peoples University
Pharmacognosy Research | Year: 2014
Objective: This study evaluated the ability of 30% ethanolic extract of Indian propolis on dentinal tubule occlusion comparatively against CPP-ACP containing desensitizing agent GC tooth mousse. Methodology: The specimens were prepared from 30 freshly extracted sound human third molars stored in 10% formalin (pH 7.0) at a room temperature. From each specimen, a sectioned sample (5 mm length × 5 mm width × 3.5 mm depth) was obtained including the cervical area. Samples were smoothened and wet-polished with 1000- and 1200-grit aluminum oxide abrasive paper and diamond pastes, in order to stimulate the clinical aspect of hypersensitive dentin cervical surfaces. All the specimens were randomly assigned to three groups (n = 10), according to dentin surface treatments. Negative control: Untreated specimens (n = 4) and pretreated with 6% citric acid (n = 6); Test Group: 30% ethanolic extract of Indian propolis (n = 10); Positive Group: GC Tooth Mousse (n =10). All the specimens were prepared for SEM analysis. Results: GC tooth mousse promoted tubule occlusion by crystal-like deposits in the lumen of the tubules. While propolis created a thin, smooth layer over dentin surface. Conclusion: According to the SEM analysis, both desensitizing agent were able to occlude the dentinal tubules.
Rakesh Kumar A.,Peoples University
Journal of Chemical and Pharmaceutical Research | Year: 2013
Antimicrobial resistance is not only increasing the healthcare costs but also the severity and death rates from certain infections that could have been avoided by prudent and rational use of the existing and newer antimicrobial agents. Prudent and rational use of antimicrobial is possible by forming local, national and global wide antibiogram. The present study is undertaken to prepare local antibiogram of Klebsiella pneumoniae (K. pneumoniae) isolated from pus and to discuss general issues related to antimicrobials use. Total 198 pus samples were processed for culture sensitivity testing. Out of 198 pus samples, 142 organisms were isolated. Staphylococcus aureus (36.7%) accounted most common organism and K. pneumoniae(21.1 %) accounted 2nd most common organism. Other organisms were Pseudomonas spp. (18.3 %), Escherichia coli (12.7 %), streptococci aureus (9.8 %) and Proteus spp. (1.4 %). Antimicrobial susceptibility testing was done by disk diffusion method described by Kirby-Bauer (1961). K. pneumonia was found to be most sensitive to amikacin, gatifloxacin, gentamicin and chloramphenicol. Consequently, considering the antimicrobial susceptibility, cost, side effects and many other factors, amikacin, gatifloxacin, gentamicin and chloramphenicol should be preferred drugs for K. pneumoniae infection isolated from pus.
Firodiya A.D.,Peoples University |
Tenguria R.K.,Government PG College
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2016
Endophytic fungi that live asymptomatically inside the plant tissues have novel bioactive metabolites exhibiting a variety of biological activities, especially against cancer. This review highlights the research progress on the production of anticancer compounds by endophytic fungi from 1990-2015. Anticancer activity is generally associated with the cytotoxicity of the compounds present in the endophytic fungi. The ubiquitous nature of endophytic fungi synthesise diverse chemicals with promising anticancer activity from either their original host or related species. Modification in fermentation parameters and genetic insight of endophytes may produce novel anti-cancerous compounds. © 2016 The Authors.
Chandrashekar B.R.,Pacific University at Udaipur |
Nagarajappa R.,Rama Dental College |
Singh R.,Peoples University |
Thakur R.,Peoples University
Journal of Young Pharmacists | Year: 2014
Introduction: To assess and compare the anti-microbial efficacy of ten herbal extracts against primary plaque colonizers. Materials and Methods: The branches of 10 plants with ethno-pharmacological background were collected and healthy leaves separated to prepare the fine powder. The extraction procedure was carried out by cold infusion method using ethanol. The working concentration of the extract (100 mg/ml) was prepared by mixing dried leaf extract with dimethyl sulfoxide (DMSO). 0.2% chlorhexidine and DMSO were used as positive and negative controls, respectively. Agar well diffusion method was used for anti-microbial efficacy testing. The statistical analysis was done using SPSS 16. The mean diameter of inhibition zone between the categories was compared using one-way analysis of variance and Tukey's post-hoc test. Results: The highest mean diameter of inhibition zone against Streptococcus mutans was produced by Acacia nilotica followed by Psidium guajava, Eucalyptus hybrid and Murraya koenigii L.S extracts in the descending order. The highest mean inhibition zone against Streptococcus sanguinis was produced by chlorhexidine followed by A. nilotica, Eucalyptus, P. guajava, and M. koenigii L.S extracts in the descending order. The highest mean inhibition zone against Streptococcus salivarius was produced by P. guajava, followed by chlorhexidine, A. nilotica, Eucalyptus, M. koenigii L.S and Hibiscus sabdariffa L extracts in the descending order. Conclusion: P. guajava, Eucalyptus, A. nilotica, M. koenigii L.S and H. sabdariffa L have the potential to inhibit primary plaque colonizers, and they could be used as anti-plaque agents.
Agrawal S.R.,Indian Institute of Management IIM |
Mittal D.,Peoples University
International Journal of Environment and Waste Management | Year: 2017
The objectives of the study are two-fold. The first is to identify the reasons of the low collection efficiency of household e-waste and the second is to suggest about an organised market with its benefits for proper handling of e-waste that suited to end-users needs. A total of 312 usable responses gathered from household end-users of EEEs of Bhopal (Madhya Pradesh), India. The study employed descriptive and regression analysis with the help of SPSS 20. The study identified the key components within this domain and found that end-users major problems and concerns regarding their e-waste can be solved significantly by online e-waste market. Also, this business model positively and significantly influences the economic, environmental, social and health benefits. The proposed business model would help to the public and private sectors for collecting and proper handling of household e-waste with economic, environmental, social and health benefits. The results of the analysis indicated that the present e-waste management system is needed to change significantly and should adopt an organised online e-waste market that would contribute to our society and the environment. Copyright © 2017 Inderscience Enterprises Ltd.
Zaidi K.U.,Peoples University
Biochemistry Research International | Year: 2014
Tyrosinase is a natural enzyme and is often purified to only a low degree and it is involved in a variety of functions which mainly catalyse the o-hydroxylation of monophenols into their corresponding o-diphenols and the oxidation of o-diphenols to o-quinones using molecular oxygen, which then polymerizes to form brown or black pigments. The synthesis of o-diphenols is a potentially valuable catalytic ability and thus tyrosinase has attracted a lot of attention with respect to industrial applications. In environmental technology it is used for the detoxification of phenol-containing wastewaters and contaminated soils, as biosensors for phenol monitoring, and for the production of L-DOPA in pharmaceutical industries, and is also used in cosmetic and food industries as important catalytic enzyme. Melanin pigment synthesized by tyrosinase has found applications for protection against radiation cation exchangers, drug carriers, antioxidants, antiviral agents, or immunogen. The recombinant V. spinosum tryosinase protein can be used to produce tailor-made melanin and other polyphenolic materials using various phenols and catechols as starting materials. This review compiles the recent data on biochemical and molecular properties of microbial tyrosinases, underlining their importance in the industrial use of these enzymes. After that, their most promising applications in pharmaceutical, food processing, and environmental fields are presented. © 2014 Kamal Uddin Zaidi et al.
Zaidi K.U.,Peoples University
Enzyme Research | Year: 2014
Melanogenesis is a biosynthetic pathway for the formation of the pigment melanin in human skin. A key enzyme, tyrosinase, catalyzes the first and only rate-limiting steps in melanogenesis. Since the discovery of its melanogenic properties, tyrosinase has been in prime focus and microbial sources of the enzyme are sought. Agaricus bisporus widely known as the common edible mushroom, it's taking place in high amounts of proteins, enzyme, carbohydrates, fibers, and low fat contents are frequently cited in the literature in relation to their nutritional value. In the present study tyrosinase fromAgaricus bisporus was purified by ammonium sulphate precipitation, dialysis followed by gel filtration chromatography on Sephadex G-100, and ion exchange chromatography on DEAE-Cellulose; the enzyme was purified, 16.36-fold to give 26.6% yield on total activity in the crude extract and final specific activity of 52.19U/mg. The SDS-PAGE electrophoresis showed a migrating protein band molecular weight of 95 kDa. The purified tyrosinase was optimized and the results revealed that the optimumvalues are pH7.0 and temperature 35°C.Thehighest activity was reported towards its natural substrate, L-DOPA, with an apparent Km value of 0.933 mM.This indicated that tyrosinase purified from Agaricus bisporus is a potential source for medical applications. Copyright © 2014 Kamal Uddin Zaidi et al.
Zhao Y.,Centers for Disease Control and Prevention |
Zhao Y.,Beijing Tuberculosis and Thoracic Tumor Research Institute |
Xu S.,Beijing Tuberculosis and Thoracic Tumor Research Institute |
Wang L.,Centers for Disease Control and Prevention |
And 14 more authors.
New England Journal of Medicine | Year: 2012
BACKGROUND: The available information on the epidemic of drug-resistant tuberculosis in China is based on local or regional surveys. In 2007, we carried out a national survey of drug-resistant tuberculosis in China. METHODS: We estimated the proportion of tuberculosis cases in China that were resistant to drugs by means of cluster-randomized sampling of tuberculosis cases in the public health system and testing for resistance to the first-line antituberculosis drugs isoniazid, rifampin, ethambutol, and streptomycin and the second-line drugs ofloxacin and kanamycin. We used the results from this survey and published estimates of the incidence of tuberculosis to estimate the incidence of drug-resistant tuberculosis. Information from patient interviews was used to identify factors linked to drug resistance. RESULTS:Among 3037 patients with new cases of tuberculosis and 892 with previously treated cases, 5.7% (95% confidence interval [CI], 4.5 to 7.0) and 25.6% (95% CI, 21.5 to 29.8), respectively, had multidrug-resistant (MDR) tuberculosis (defined as disease that was resistant to at least isoniazid and rifampin). Among all patients with tuberculosis, approximately 1 of 4 had disease that was resistant to isoniazid, rifampin, or both, and 1 of 10 had MDR tuberculosis. Approximately 8% of the patients with MDR tuberculosis had extensively drug-resistant (XDR) tuberculosis (defined as disease that was resistant to at least isoniazid, rifampin, ofloxacin, and kanamycin). In 2007, there were 110,000 incident cases (95% CI, 97,000 to 130,000) of MDR tuberculosis and 8200 incident cases (95% CI, 7200 to 9700) of XDR tuberculosis. Most cases of MDR and XDR tuberculosis resulted from primary transmission. Patients with multiple previous treatments who had received their last treatment in a tuberculosis hospital had the highest risk of MDR tuberculosis (adjusted odds ratio, 13.3; 95% CI, 3.9 to 46.0). Among 226 previously treated patients with MDR tuberculosis, 43.8% had not completed their last treatment; most had been treated in the hospital system. Among those who had completed treatment, tuberculosis developed again in most of the patients after their treatment in the public health system. CONCLUSIONS: China has a serious epidemic of drug-resistant tuberculosis. MDR tuberculosis is linked to inadequate treatment in both the public health system and the hospital system, especially tuberculosis hospitals; however, primary transmission accounts for most cases. (Funded by the Chinese Ministry of Health.) Copyright © 2012 Massachusetts Medical Society.