Entity

Time filter

Source Type


Gong J.-Q.,Peoples Liberation Army General Hospital of Chengdu Command | Ren J.-D.,Peoples Liberation Army General Hospital of Chengdu Command | Tian F.-Z.,Peoples Liberation Army General Hospital of Chengdu Command | Jiang R.,General Hospital of Chengdu Command | And 2 more authors.
World Journal of Gastroenterology | Year: 2011

To propose a new classifcation system for sphincter of Oddi dysfunction (SOD) based on clinical data of patients. METHODS: The clinical data of 305 SOD patients documented over the past decade at our center were analyzed retrospect tively, and typical cases were reported. RESULTS: The new classifcation with two more types (double-duct, biliary-pancreatic reflux) were set up on the basis of the Milwaukee criteria. There were 229 cases of biliary-type SOD, including 192 (83.8%) cases cured endoscopically, and 29 (12.7%) cured by open abdominal surgery, and the remaining 8 (3.5%) cases observed with unstable outcomes. Eight (50%) patients with pancreatic-type SOD were cured by en-doscopic treatment, and the remaining 8 patients were cured after open abdominal surgery. There were 19 cases of double-duct-type SOD, which consisted of 7 (36.8%) patients who were cured endoscopically and 12 (63.2%) who were cured surgically. A total of 41 cases were diagnosed as biliary-pancreatic-reflux-type SOD. Twenty (48.8%) of them were treated endoscopically, 16 (39.0%) were treated by open abdominal surgery, and 5 (12.2%) were under observation. CONCLUSION: The newly proposed SOD classification system introduced in this study better explains the clinical symptoms of SOD from the anatomical perspective and can guide clinical treatment of this disease. © 2011 Baishideng. All rights reserved. Source


Gong J.,Peoples Liberation Army General Hospital of Chengdu Command | Tian F.,Peoples Liberation Army General Hospital of Chengdu Command | Ren J.,Peoples Liberation Army General Hospital of Chengdu Command | Luo G.,Peoples Liberation Army General Hospital of Chengdu Command
Pancreatology | Year: 2010

Purpose: To investigate the origin and localization of pancreatic stem cells in adult pancreatic tissues and to determine the primary mechanism underlying the participation of these cells in repairing pancreatic injuries. Methods: Sprague-Dawley rats were divided into experimental and control groups. The experimental group was given intraperitoneal injections of cerulein to induce acute pancreatitis. At 6 h, 1, 2, 3, 5 and 7 days, 5 rats from the experimental group and 2 rats from the control group were sacrificed; all sacrificed animals were intraperitoneally injected with 5-bromo-2′- deoxyuracil nucleotides (BrdU) 6 and 3 h prior to sacrifice. The pathological changes of pancreatic tissue were observed. The stem cell marker nestin and the cell proliferation marker BrdU were detected with immunohistochemistry. Pancreatic duodenal homeobox-1 (PDX-1) was determined by real-time PCR. Results: (1) The pathological changes of acute pancreatitis can be divided into three phases: the edema and apoptosis phase, the hemorrhagic necrosis phase, and the reconstruction phase. (2) Nestin-positive cells mainly appeared in the interlobular vascular lumen after cerulein injection, and they peaked at day 3 when the positive cells spread all over the pancreatic tissues. (3) BrdU-positive cells began to appear in the area surrounding the interlobular region, and the number of positive cells peaked on day 7. (4) The expression of PDX-1 mRNA initially increased, then decreased and gradually got close to a normal level. Conclusion: Primary pancreatic stem cells may not exist in the adult pancreatic tissues. The so-called pancreatic stem cells may actually originate from bone marrow stem cells. When pancreatic tissue is injured, bone marrow stem cells may participate in the repair. Copyright © 2010 S. Karger AG, Basel and IAP. Source


Gong J.,Peoples Liberation Army General Hospital of Chengdu Command | Zhang G.,Peoples Liberation Army General Hospital of Chengdu Command | Tian F.,Peoples Liberation Army General Hospital of Chengdu Command | Wang Y.,Peoples Liberation Army General Hospital of Chengdu Command
Journal of Molecular Histology | Year: 2012

This study investigated the role of adult islet-derived stem cells in repairing islet damage. Using intraperitoneal injection of cerulein, a rat model of acute pancreatitis was induced in an experimental group. The expression of the protein c-kit, a pancreatic stem cell marker, was observed using immunohistochemistry at set intervals following successful model preparation. Pathological changes in pancreatic tissues were also observed using routine hematoxylin and eosin staining. Cells with positive c-kit staining were rarely observed in normal tissues. At all observation intervals after the induction of acute pancreatitis, c-kit staining was restricted to the islets. Over the course of observations, staining changed from low to high intensity, and then back to low intensity again. The primary pathological manifestation in the experimental group was edematous pancreatic tissues with local necrotic lesions. These findings suggested that c-kit positive cells are likely to be a type of pancreatic progenitor cell that is involved only in the self-repair of islet damage and does not migrate. © 2012 Springer Science+Business Media B.V. Source

Discover hidden collaborations