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Lu F.,Peoples Hospital of Zunhua City | Zhang H.-T.,Peoples Hospital of Zunhua City | Ma S.-Y.,Peoples Hospital of Zunhua City
Chinese Journal of Tissue Engineering Research

Background: Polyanhydride material is characteristics of surface erosion, biodegradability and release rate adjustability, and it has been approved by the U.S. Food and Drug Administration for human drug carrier materials. Objective: To prepare gatifloxa-poly(sebacic anhydride) local control release system and study its biocompatibility. Methods: Poly(sebacic anhydride) was prepared with melt phase polycondensation method, and mixed with gatifloxacin in the agate mortar to make a local drug delivery system at 20% drug loading. Six rabbits were used to study the biocompatibility of poly(sebacic anhydride) and their back skin was cut open. Six rabbits were randomly divided into two groups, the experimental group was implanted with poly(sebacic anhydride) stick into the paraspinal muscle pouch, while the control group received no implants. Rabbits were killed at 5 weeks post-surgery, and changes on subcutaneous tissue and muscle tissue were observed, as well heart, liver, kidney, and lung. Results and Conclusion: Poly(sebacic anhydride) has a good biocompatibility. After implantation, no rabbit appeared to have the change of appetite and behavior. There was no edema, hemorrhage and erosion at the implanted site, and the surface of implanted materials was porous, which suggested that the materials had been degraded and absorbed subcutaneously. In addition, embrittlement and disintegration were not observed; the implanted materials slightly adhered to surrounding tissue. There was no change in the liver, kidney, lung, heart and partial musculature. Gatifloxacin-poly(sebacic anhydride) local sustained release preparation is well histocompatible with no toxicity and teratogenic action. Source

Lu F.,Peoples Hospital of Zunhua City | Yan X.-Z.,Peoples Hospital of Zunhua City | Ma S.-Y.,Peoples Hospital of Zunhua City | Zhang H.-T.,Peoples Hospital of Zunhua City
Chinese Journal of Tissue Engineering Research

BACKGROUND: Polyanhydride materials characterized as surface corrosion resistance, biodegradability and adjustable release rate have been used as drug delivery materials for the body, but the application of polysebacic anhydride delivery system has been rarely reported. OBJECTIVE: To study the role of gatifloxa-polysebacic anhydride local delivery system in the prevention of osteomyelitis in rabbits. METHODS: According to Norden method, we made Staphylococcus aureus-induced osteomyelitis models in the femur of 30 rabbits. There were three groups in the study, 10 rats in each group. Gatifloxa-polysebacic anhydride local delivery system and gatifloxa were respectively used in comparison with model group. After modeling, general observation, anatomical observation, X-ray examination, bone culture and bacteria counting, and histological examination were performed. RESULTS AND CONCLUSION: After 8 weeks, animals in the model group presented with poor appetite, rough fur, and weight loss. Among these 10 rabbits, 3 rabbits died within 3 weeks, and another 7 rabbits appeared to have severe periosteal reactions on the X-ray films, that is, abscess formation shown in the anatomical examination and visible manifestation of osteomyelitis through bone culture and histological examination. In the gatifloxa group, two rabbits died within 3 weeks, abscess formation was found in four rabbits, and periosteal reactions were mild without bone sequestration. Six of 10 rabbits in the gatifloxa appeared to have manifestation of osteomyelitis. In the delivery system group, the animals had a good general state of health and no abnormality was found in the anatomical examination, X-ray examination, bone culture and bacteria count, and histological examination. These findings indicate that the gatifloxa-polysebacic anhydride local sustained release preparation could effectively prevent osteomyelitis. Source

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