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Xia L.,Peoples Hospital Of Zhucheng | Wang H.,Weifang Peoples Hospital | Sun Y.,Weifang Medical University | Pang J.,Weifang Peoples Hospital
International Journal of Clinical and Experimental Medicine | Year: 2016

Although important for advanced life support and critical care, mechanical ventilation frequently caused ventilator-induced lung injury (VILI), manifesting a severe impact on survival rate. As the new generation of immunosuppressant, efalizumab was studied on a VILI model in rats to elucidate the protective function against damage and possible mechanisms. SD rats were randomly enrolled into control, VILI model, IgG control, glucocorticoid and efalizumab treated groups (N=15 each). The VILI model was generated by mechanical ventilation. In the experimental group, 2.5 mg/kg efalizumab was applied before mechanical ventilation. The total number of nuclear cells and neutrophils in bronchoalveolar lavage fluid (BALF) were counted, and the expressions of tumor necrosis factor (TNF)-α and interleukin (IL)-8 were also determined. Real-time PCR and Western blotting were also employed to detect the expression levels of SP-A gene and protein. Both nuclear cell and neutrophil numbers were significantly increased in VILI model group (P<0.05). The intervention by efalizumab decreased inflammatory cell number, as well as impeding the levels of cytokines such as TNF-α and IL-8 (P<0.05 in all cases). In VILI and IgGgroups, mRNA levels of SP-A gene were significantly decreased (P<0.05) but were potentiated by the addition of efalizumab or glucocorticoid. SP-A proteins had consistent distribution patterns as those of mRNA did. Efalizumab protects lung tissues from VILI via decreasing the activation and infiltration of inflammatory cells, inhibiting inflammatory factor release and facilitating expression of surfactant proteins. © 2016, E-Century Publishing Corporation. All rights reserved.


Cao Y.,Central University of Costa Rica | Wang Z.,Peoples Hospital of Zhucheng | Gu J.,Central University of Costa Rica | Hu F.,Central University of Costa Rica | And 5 more authors.
BioMed Research International | Year: 2015

Many studies have investigated the association between hormonal and reproductive factors and thyroid cancer risk but provided contradictory and inconclusive findings. This review was aimed at precisely estimating this association by pooling all available epidemiological studies. 25 independent studies were retrieved after a comprehensive literature search in databases of PubMed and Embase. Overall, common hormonal factors including oral contraceptive and hormone replacement therapy did not alter the risk of thyroid cancer. Older age at menopause was associated with weakly increased risk of thyroid cancer in overall analysis (RR = 1.24, 95% CI 1.00-1.53, P = 0.049); however, longer duration of breast feeding was related to moderately reduced risk of thyroid cancer, suggested by pooled analysis in all cohort studies (RR = 0.7, 95% CI 0.51-0.95, P = 0.021). The pooled RR in hospital-based case-control studies implicated that parous women were more susceptible to thyroid cancer than nulliparous women (RR = 2.30, 95% CI 1.31-4.04, P = 0.004). The present meta-analysis suggests that older age at menopause and parity are risk factors for thyroid cancer, while longer duration of breast feeding plays a protective role against this cancer. Nevertheless, more relevant epidemiological studies are warranted to investigate roles of hormonal and reproductive factors in thyroid carcinogenesis. © 2015 Yijuan Cao et al.


Zhao Y.-B.,Peoples Hospital of Changle | Zhang S.-H.,Peoples Hospital of Zhucheng | Wang J.-Q.,Peoples Hospital of Zhucheng | Cai X.-H.,Peoples Hospital of Zhucheng | And 2 more authors.
Chinese Journal of Cancer Prevention and Treatment | Year: 2011

OBJECTIVE: To study the protein expressions of EphA2 and EphrinA1 in breast cancer tissues and to explore their significance. METHODS: Using immunohistochemistry Sp method to detect the protein expressions of EphA2 and EphrinA1 in cancer tissues from 130 breast cancer patients. Then studying their relationship to clinicopathological factors. RESULTS: Among the 130 breast carcinoma cases, The total positive rate of EphA2 and EphrinA1 protein was 72. 31% and 59. 23%. The positive expression of EphA2 protein were associated with clinical stage, lymph node metastasis and histological grade(P<0. 05). The positive expression of EphrinAl protein were significantly correlated with clinical stage and histological grade(P<0. 05). The positive staining of EphA2 and EphrinAl protein co-located in roughly the same tumor areas and vascular endothelial cells. Their distribution were fundamentally unanimous and their expression were positively correlated (P<0. 05). CONCLUSIONS: EphA2 and EphrinA1 may be involved in carcinogenesis and development procedures of breast carcinoma. The combined detection of EphA2 and EphrinA1 can help to predict the clinical and pathologic characteristics of breast carcinoma. It is an important index to guide treatment, and they are predictive factors for breast cancer.


Zhao Y.-B.,Southern Medical University | Zhang S.-H.,Peoples Hospital of Zhucheng | Zhang J.-R.,Southern Medical University
Journal of Practical Oncology | Year: 2010

Objective: To investigate the expressions of ER, PR and EphA2 in breast cancers and their clinical significance. Methods: The expressions of ER, PR and EphA2 were detected in 130 cases of breast career by immunohistochemistry S-P method. Results: (1) The positive rates of expressions of ER, PR and EphA2 were 63.1%, 58.4% and 72.3%, respectively. (2) No significant association of expression of ER and PR was noted with age, histological types, tumor size, lymphatic metatasis and clinical stages. (3) The expression of EphA2 was related to clinical stages and lymphatic metatasis(P < 0.05), but not to age, tumor size and histological types(P > 0.05). (4) The positive rate of expression of EphA2 in the group with the positive expression of ER and PR was lower than that in the group with negative expression of ER and PR(P < 0.05). Conclusions: ER, PR and EphA2 are related with the occurrence and development of breast cancer. The combined detection of ER, PR and EphA2 can help to predict the clinical and pathologic characteristics of breast career, which is an important index to guide treatment. ER, PR and EphA2 are predictive factors for breast cancer.


Cai X.-H.,Peoples Hospital of Zhucheng | Zhao Y.-B.,Peoples Hospital of Zhucheng | Zhang S.-H.,Peoples Hospital of Zhucheng | Wang J.-Q.,Peoples Hospital of Zhucheng | And 2 more authors.
Chinese Journal of Cancer Prevention and Treatment | Year: 2011

OBJECTIVE: To investigate the expressions of EphB4 and EphrinB2 protein in endometrial carcinomas, and study their significance. METHODS: Using immunohistochemistry Sp method to detect the expressions of EphB4 and EphrinB2 protein in tumors from 71 endometrial carcinomas. Then study their relationship to clinicopathological factors. RESULTS: Among the 71 endometrial carcinoma cases, The total positive rate of EphB4 and EphrinB2 protein was 69.3% and 64.9%. The positive expression of EphB4 protein were associated with age, clinical stage, lymph node metastasis and histological grade (P < 0.05). The positive expression of EphrinB2 protein were significantly correlated with lymph node metastasis and histological grade(P < 0.05). The positive staining of EphB4 and EphrinB2 were positively correlated (P < 0.05). CONCLUSIONS: EphB4 and EphrinB2 may be involved in occurence and development of endometrial carcinoma. The combined detection of EphB4 and EphrinB2 can help to predict the clinical and pathologic characteristics of endometrial carcinoma.


Qu G.,Ocean University of China | Yue X.,Ocean University of China | An F.,Peoples Hospital of Zhucheng | Dai S.,Yantai University | And 2 more authors.
Zhongguo Zhongyao Zazhi | Year: 2012

Objective: To investigate chemical constituents contained in Salvia castanea. Method: The compounds were separated and purified by silica gel, macroporous resin, RP-C18 and Sephadex LH-20 column chromatography. The structures were identified on the basis of physicochemical property and spectral data. Result: Nineteen compounds were separated and identified as tanshinone IIA (1), tanshinone IIB (2), hydroxytanshinone IIA (3), tanshinone I(4), dihydrotanshinone I(5), cryptotanshinone (6), neotanshinone A(7), neotanshinone B(8), tanshinoldehyde(9), przewaquinone A(10), przewaquinone B(11), sugiol(12), caffeic acid(13), rosmarinci acid(14), ethyl rosmarinate(15), lithospermic acid(16), pro-lithospermic acid(17), protocatechualdehyde (18), and danshensu(19). Conclusion: Compounds 2, 3, 7-13 and 15-19 were separated from S. castanea for the first time.


Zhang R.,Peoples Hospital of Zhucheng
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2013

To investigate the expressions of EphrinB2 and VEGF in nasopharyngeal carcinoma and their relationships with clinic pathological factors. The expressions of EphrinB2 and VEGF in 136 cases of nasopharyngeal carcinomas, and 20 cases of nasopharyngitis tissues were detected by SP method immunohistochemistry. 1) The positive rates of EphrinB2 and VEGF were 63.2% and 67.6% respectively in 136 cases of nasopharyngeal carcinomas. Their positive rate in nasopharyneal carcinoma tissues was significantly higher than those in nasopharyngitis tissues (P<0.01). 2) Intensity of the expression of EphrinB2 and VEGF protein was related to lymphnodes metabasis, cranial nerve palsy, basalis encroachment, clinical stage respectively (P<0.01). 3) The expression of EphrinB2 in nasopharyngeal carcinoma tissues and was positively correlated with VEGF (P< 0.01). Ephrinbeta2 and VEGF may play important roles in invasiveness, metastasis and angiogenesis of nasopharyngeal carcinoma. There may be certain inter regulation mechanism between them and they are hoped to become new biologic parameters to judge the pathogenesis, development and prognosis of nasopharyngeal carcinoma and to guide the treatment.


Wang J.-Q.,Peoples Hospital of Zhucheng | Zhao Y.-B.,Peoples Hospital of Zhucheng | Liang Y.-Q.,Peoples Hospital of Zhucheng
Journal of Practical Oncology | Year: 2010

Objective: To investigate microwave ablation combined with endostatin in treatment of human kidney carcinoma transplanted in nude mice. Methods: Human kidney Ketr-3 kidney cells were subcutaneously implanted in 48 BalB/C-nu/nu mice. When the diameter of tumor reached 1.5 cm the animals were randomly divided into 4 groups: in microwave group the mice were treated with microwave ablation; in endostatin group endostatin was injected through tail vein; in combination group the mice received endostatin injection and microwave ablation; in control group normal saline was injected. The tumor weight and the inhibition rate were calculated, and the survival time was observed. Plasma concentrations of vascular endothelial growth factor (VEGF) of tumors were measured by ELISA method. Results: The tumor weight was lower in the combined treatment group than in the other groups (P < 0.05). Plasma VEGF in combined treatment group was significantly decreased compared with that of other groups (P < 0.05). Conclusion: Microwave ablation combined with endostatin shows better therapeutical effects than each method used alone in treatment of transplanted human kidney carcinoma in nude mice.


PubMed | Weifang Medical University, Peoples Hospital of Zhucheng and Central University of Costa Rica
Type: | Journal: BioMed research international | Year: 2015

Many studies have investigated the association between hormonal and reproductive factors and thyroid cancer risk but provided contradictory and inconclusive findings. This review was aimed at precisely estimating this association by pooling all available epidemiological studies. 25 independent studies were retrieved after a comprehensive literature search in databases of PubMed and Embase. Overall, common hormonal factors including oral contraceptive and hormone replacement therapy did not alter the risk of thyroid cancer. Older age at menopause was associated with weakly increased risk of thyroid cancer in overall analysis (RR = 1.24, 95% CI 1.00-1.53, P = 0.049); however, longer duration of breast feeding was related to moderately reduced risk of thyroid cancer, suggested by pooled analysis in all cohort studies (RR = 0.7, 95% CI 0.51-0.95, P = 0.021). The pooled RR in hospital-based case-control studies implicated that parous women were more susceptible to thyroid cancer than nulliparous women (RR = 2.30, 95% CI 1.31-4.04, P = 0.004). The present meta-analysis suggests that older age at menopause and parity are risk factors for thyroid cancer, while longer duration of breast feeding plays a protective role against this cancer. Nevertheless, more relevant epidemiological studies are warranted to investigate roles of hormonal and reproductive factors in thyroid carcinogenesis.

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