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Li X.-L.,Peoples Hospital of Zhengzhou City
Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology | Year: 2013

Background: Leber hereditary optic neuropathy (LHON) is a disease characterized by maternal inheritance. A number of mitochondrial DNA (mtDNA) mutation has been thought to be associated with this disease. Objective: This study was to investigate the clinical and molecular genetic properties of LHON in two Chinese families. Methods: Forty subjects from two Chinese families with LHON were enrolled in Affiliated First Hospital of Zhengzhou University, including 28 maternal members (10 of these members are LHON and 12 controls from two families. All the participants had a complete ophthalmic examination including visual acuity, direct ophthalmoscopy, color sensation and visual evoked potentials. MtDNA was extract from the whole blood sample of all participants. PCR-DNA sequencing was performed to detect the point mutation of the Gl 1778A, T14484C, and G3462A for each subject. Written informed consent was obtained from each subject prior to this study. Results: Only G1l778A point mutation was identified in all 28 maternal members from the two families. No point mutation of G11778A was identified in non-maternal members, and no point mutation of the T14484C and G3462A were found in the two families. Conclusions: The inherited pattern of these two families shows typical clinical and genetic features of LHON. LHON patients with G11778A mutation have a poor prognosis of visual acuity. Copyright © 201 3by the Chinese Medical Association.


Yuan H.-J.,Henan Provincial Peoples Hospital | Yang X.-G.,Peoples Hospital of Zhengzhou City | Shi X.-Y.,Henan Provincial Peoples Hospital | Tian R.,Henan Provincial Peoples Hospital | Zhao Z.-G.,Henan Provincial Peoples Hospital
Chinese Medical Journal | Year: 2011

Background Previous studies have demonstrated that serum uric acid (UA) is an independent predictor of incident type 2 diabetes mellitus (T2DM) in general populations. This study aimed to investigate specific characteristics of UA and its relationship between UA and blood glucose and other risk factors in the Chinese population. Methods A total of 946 subjects were included in this study. UA, glucose, insulin, fractional excretion of UA (FEua), creatinine clearance rate (Ccr), hemoglobin A1c (HbA1c), fructosamine (FA), blood pressure and lipids were studied and also reexamined after the patients underwent two weeks of combined therapeutics. Results UA levels were the highest in subjects with impaired glucose regulation (IGR), followed by subjects with normoglycemia (NGT) and finally by subjects with T2DM. The level of the 2-hour postprandial insulin and the area under the curve for insulin (AUCins) showed a similar tendency. The UA levels initially increased with increasing fasting blood glucose (FBG) and postprandial blood glucose (PPBG) levels, up to 7 mmol/L and 10 mmol/L, respectively, and thereafter decreased at higher FBG and PPBG levels. Compared with subjects in the lower serum UA quartile, subjects in the upper quartile of serum UA levels had higher weights, triglyceride levels, and creatinine levels as well as lower Ccr and FEua levels. Compared with women's group, UA levels were higher, and FEua levels were lower in men's group. Sex, body mass index (BMI), mean arterial blood pressure (MAP), serum triglycerides (TG), FA and Ccr were independent correlation factors of UA. UA decreased and FEua increased after the patients underwent a combined treatment. Conclusions UA increased initially and then decreased as glucose levels increased from NGT to IGR and T2DM. Compared with NGT and T2DM, IGR subjects had higher SUA levels, which related to its high levels of insulin. Under T2DM, male gender, BMI, MAP, Ccr, TG and FA are independent correlation factors of UA. Glucose-lowering, antihypertensive, lipemia-regulating combined treatments were of advantage to decline of SUA of T2DM.


Zhou J.,Peoples Hospital of Zhengzhou City | Zhang F.,Peoples Hospital of Zhengzhou City | Hou X.,Peoples Hospital of Zhengzhou City | Zhang N.,Peoples Hospital of Zhengzhou City
Cancer Biotherapy and Radiopharmaceuticals | Year: 2014

Leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC) is a multifunctional protein involved in the mitochondrial gene expression and function, cell cycle progression, and tumorigenesis. However, the functional role of LRPPRC in prostate cancer (PCa) has not yet been elucidated. In this study, two PCa cell lines were examined to determine the effects of LRPPRC on cell proliferation, invasion, and apoptosis in vitro. Our results showed that the expression levels of LRPPRC were significantly decreased in the two PCa cell lines after transfection with small interfering RNA (siRNA)-LRPPRC. Knockdown of LRPPRC by siRNA significantly inhibited the invasion and promoted the apoptosis of PCa cells. In addition, downregulation of LRPPRC expression resulted in the reduced expression of Bcl-2, upregulation of Bax, and cleaved caspase-9 and caspase- 3. Taken together, these results show that the downregulation of LRPRPC expression induces apoptosis through the mitochondria-mediated pathway in PCa cells. These experimental data seem to suggest that LRPPRC plays a critical role in the development of PCa, and its inhibition could present a potential molecular approach for the treatment of PCa. © Mary Ann Liebert, Inc.


PubMed | Peoples Hospital of Zhengzhou City
Type: Journal Article | Journal: Cancer biotherapy & radiopharmaceuticals | Year: 2014

Leucine-rich pentatricopeptide repeat motif-containing protein (LRPPRC) is a multifunctional protein involved in the mitochondrial gene expression and function, cell cycle progression, and tumorigenesis. However, the functional role of LRPPRC in prostate cancer (PCa) has not yet been elucidated. In this study, two PCa cell lines were examined to determine the effects of LRPPRC on cell proliferation, invasion, and apoptosis in vitro. Our results showed that the expression levels of LRPPRC were significantly decreased in the two PCa cell lines after transfection with small interfering RNA (siRNA)-LRPPRC. Knockdown of LRPPRC by siRNA significantly inhibited the invasion and promoted the apoptosis of PCa cells. In addition, downregulation of LRPPRC expression resulted in the reduced expression of Bcl-2, upregulation of Bax, and cleaved caspase-9 and caspase-3. Taken together, these results show that the downregulation of LRPRPC expression induces apoptosis through the mitochondria-mediated pathway in PCa cells. These experimental data seem to suggest that LRPPRC plays a critical role in the development of PCa, and its inhibition could present a potential molecular approach for the treatment of PCa.

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