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Liao Y.-S.,Peoples Hospital of Sichuan Province
International Eye Science | Year: 2016

AIM: To investigate the risk factors and prognosis of posterior capsular rupture in phacoemulsification cataract surgery through analyzing the cataract patients in our hospital. METHODS: Totally 1825 patients (1912 eyes) treated in our hospital were selected from November 2012 to November 2015. The patients with posterior capsular rupture were as observation group. Control group was randomly selected with a 1:4 ratio in patients without posterior capsular rupture. All the patient's age, gender, type of cataract, ocular disease history, other medical history, surgical history, preoperative intraocular pressure, the eyes affected, the health situation, visual acuity at 1d before and after surgery and other factors were recorded and analyzed for risk factors and prognosis. RESULTS: Statistical results showed gender, type of cataract, preoperative intraocular pressure, history of tobacco and alcohol, eyes affected and other factors did not impact on the rupture rate (P>0.05); and type of nucleus, preoperative visual acuity, some eye diseases were the risk factors for posterior capsule rupture (P<0.05). Postoperative visual acuity of the two groups was all improved, but visual acuity of patients with posterior capsule rupture significantly decreased. CONCLUSION: With eye disease history (a history of vitreous hemorrhage, retinal detachment before surgery and preoperative visual acuity <20/200) are more likely to result in intraoperative posterior capsular rupture. Nuclear hardness over grade IV is also an important risk factor. Before one surgery, it is needed to learn more about the medical history of cataract patients, to conduct a reasonable comprehensive assessment for risk factors, to decrease the risk in the surgery, which are to increase the success rate and to improve the prognosis. Copyright 2016 by the IJO Press.

Tang Q.,Peoples Hospital of Sichuan Province
International Eye Science | Year: 2016

AIM: To study the application value and therapeutic effect of small incision trabeculectomy in tunnel in cataract combined with glaucoma surgery. METHODS: Eighty-two patients of ninety eyes with cataract combined with glaucoma were selected in our hospital from May 2013 to May 2015, all of them treatment of small incision trabeculectomy in tunnel surgery, compare the difference of intraocular pressure and refractive degree at different time points before and after operation; To analyze the improvement of visual acuity after surgery and the occurrence of complications. RESULTS: The intraocular pressure of postoperative 1, 7d, 1 and 6mo (19.38±3.63, 12.96±2.84, 11.37±1.05, 11.89±0.82mmHg) were lower than preoperative (32.65±6.42mmHg), and the difference has statistical significance (P<0.05). The vertical corneal refraction first increased and then decreased after the operation. The difference time points were higher than the preoperative, the difference was statistically significant (P<0.05). The horizontal corneal refraction first decreased and then increased after surgery. The horizontal corneal refraction at each time point except 1mo after surgery and preoperative differences were statistically significant. Five eyes (6%) of patients with vision <0.1 after 6mo of operation, forty-eight eyes (53%) with vision 0.1-0.5, 29 eyes (32%) with vision 0.6-0.9, eight eyes (9%) with vision >1.0, the vision was significantly improved after 6mo of operation (P<0.05). There were 3 eyes of patients with anterior chamber, 5 eyes had corneal edema, 3 eyes had the appearance of anterior chamber, and recovered within 7d after treatment. CONCLUSION: The small incision trabeculectomy in tunnel for cataract combined with glaucoma has significant curative effect, higher safety, and highly clinical value. Copyright 2016 by the IJO Press.

Yu L.,Peoples Hospital of Sichuan Province | Ran B.,Changhai Hospital | Li M.,Peoples Hospital of Sichuan Province | Shi Z.,Changhai Hospital
Spine | Year: 2013

Study Design: Systematic review and meta-analysis. Objective: To review the literature systematically and make a comprehensive understanding of the efficacy of these 2 drugs in the management of postoperative pain after lumbar spinal surgery. Summary of Background Data: Several trials that evaluated the efficacy of gabapentin and pregabalin in the management of postoperative pain after lumbar spinal surgery have been published. Methods: PubMed (1980 to present), adapted for EMBASE (1980 to present), and Cochrane databases were searched for randomized placebo-controlled trials. Random effect model was used in our meta-analysis, and standard mean difference (SMD) was chosen as the pooled estimate. Results: Seven trials were included in our study. All included studies could be considered to be of high quality in methodology. The pooled results from meta-analysis demonstrated that compared with placebo, both gabapentin and pregabalin could significantly reduce the postoperative narcotic consumption (SMD,-1.54, and-1.16, respectively). Oral gabapentin was efficacious in the management of postoperative pain at all time points during the first day after surgery (SMD,-1.91 at 0-6 hr,-1.30 at 6-12 hr,-1.05 at 12-24 hr, respectively). Pregabalin seemed to be also efficacious in the management of postoperative pain at 0 to 6 hours (SMD,-1.05), at 6 to 12 hours (SMD,-0.62), and at 12 to 24 hours (SMD,-0.43). Both drugs could be well tolerated in our included trials, compared with placebo. Conclusion: This work suggested that both gabapentin and pregabalin were efficacious in reduction of postoperative pain and narcotic requirements after lumbar spinal surgery, whereas more trials were needed to further assess the efficacy of pregabalin in the management of postoperative pain after lumbar spinal surgery. © 2013 Lippincott Williams & Wilkins.

Qiu L.,Peoples Hospital of Sichuan Province
Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2013

To observe the long-term efficacy and safety of warm needling therapy combined with rehabilitation training in the treatment of knee osteoarthritis (KOA) for the patients living in simple room after earthquake. Eighty-eight cases of KOA were randomized into a warm needling group and an acupuncture group, 44 cases in each one. In the warm needling group, the warm needling therapy was applied at Neixiyan (EX LE 4) and Dubi (ST 35), in combination with rehabilitation training. In the acupuncture group, the same rehabilitation training was received, and the same acupoints were selected as the warm needling group, but only stimulated with acupuncture without moxibustion applied. The clinical efficacy was observed after 20 treatments and in 1-2 months of follow-up. The total effective rate was 100.0% (41/41) in the warm needling group, which was apparently superior to 85.4% (35/41) in the acupuncture group (P < 0.05). The symptom integral, pain and knee joint function score were all improved significantly after 20 treatments in both groups (all P < 0.05). The improvements in symptoms and pain in the warm needling group were superior apparently to the acupuncture group (all P < 0.05). The efficacy remained well within 2 months follow-up (all P < 0.05). But the difference in knee joint function score was not statistically significant between the two groups (all P > 0.05). The combined therapy of warm needling and rehabilitation training achieves the superior efficacy and good safety in the treatment of KOA as compared with the combined therapy of simple acupuncture and rehabilitation training for the patients living in simple room after earthquake.

AIM: To evaluate application value of recombinant bovine basic fibroblast growth factor on dry eye after cataract phacoemulsification combined with intraocular lens implantation. METHODS: One hundred and forty patients were selected with one dry eye after the surgery above, and randomly divided into control group and observation group. The two groups were treated with polyethylene glycol eye drops and polyethylene glycol eye drops combined with recombinant bovine basic fibroblast growth factor eye gel respectively. We tested their dry eye symptom score, Schirmer I test (SIt); tear break-up time (BUT) and corneal fluorescein staining before and 1mo after treatment. Finally, statistical analysis of the two groups of efficacy differences were carried out. RESULTS: Before treatment, there was no significant difference in dry eye symptom score, SIt and BUT between two groups (P>0.05). After treatment, the three measured values above were significantly improved within each group by Paired t-test (P<0.05), while only the dry eye symptom score and BUT in observation group were significantly better than the control group (P<0.05). The decrease rate of corneal fluorescein staining score in the observation group was significantly higher than that in control group (P<0.05). CONCLUSION: For the treatment of dry eye after cataract phacoemulsification combined with intraocular lens implantation, combined application of the traditional artificial tears polyethylene glycol and recombinant bovine basic fibroblast growth factor is more effective than those only using polyethylene glycol. Combined treatment can improve the eye discomfort, prolong the time of BUT, and reduce the corneal fluorescein staining score more significantly. The medicine has certain application value in clinical. Copyright 2016 by the IJO Press.

To study the regulatory effect of thymosin α1 (Tα1) on immunosuppression of bone marrow mesenchymal stem cells (MSCs) from children with aplastic anemia (AA) through Toll-like receptor 9(TLR9)and indoleamine 2,3-dioxygenase (IDO) signaling pathway. Bone marrow T cell subsets from children with AA and normal individuals were measured by using flow cytometry. Expressions of TLR9/IDO mRNA of MSCs cocultured with Tα1 were determined by reverse-transcription PCR (RT-PCR). Inhibition of PHA-activated T cell proliferation and activation by MSCs cocultured with Tα1 was detected by using MTT assay and flow cytometry. CD4(+)/CD8(+) ratio (0.64 ± 0.02) in children with AA was significantly lower than that in normal individuals (1.42 ± 0.05); but CD8(+)/CD38(+) ratio (0.92 ± 0.04) was significantly higher than that in normal individuals (0.65 ± 0.05). AA MSCs obviously expressed TLR9, but not IDO; AA MSCs treated with Tα1 downregulated TLR9 expression but upregulated IDO expression in concentration- and time-dependent manners. The inhibition of AA MSCs on T cell proliferation (21.38% ± 12.34%) was lower than that in normal individuals (62.72% ± 17.79%, P < 0.05), while AA MSCs treated with Tα1 for 18 h exhibited a stronger inhibition (42.83% ± 16.54%, P < 0.05). The immunosuppression mediated by MSCs could be improved by Tα1 through upregulation of IDO expression via TLR9-dependent signaling pathway. This research provides a new idea for targeted immunomodulatory therapy with bone marrow MSCs from children with AA.

Liu L.,Peoples Hospital of Sichuan Province
Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2010

To assess the efficacy of quick meridian needling therapy plus scalp acupuncture on infantile cerebral palsy (CP). One hundred and forty-seven cases of CP were randomly divided into a quick meridian needling therapy plus scalp acupuncture group (group A), a conventional acupuncture group (group B) and a scalp acupuncture group (group C), 49 cases in each one. In group A, quick needling was applied to the Conception Vessel, Governor Vessel, Hand-Yangming, Hand-Jueyin, Foot-Yangming and Foot-Taiyin meridians distributed on four limbs and trunk. One pricking point was 10 mm far from the other one. In scalp acupuncture, motor area, equilibrium area, sensory area, tremor-control area, foot-motor-sensory area, speech No. 2 area, speech No. 3 area, Baihui (GV 20), Sishencong (EX-HN 1), etc. were selected. The needles were stimulated with rotating manipulation and remained for 30-60 min. In group B, the conventional acupuncture was adopted mainly at Dazhui (GV 14), Shenzhu (GV 12), Fengfu (GV 16) and others. In group C, the scalp acupuncture was used and the points selected were same as those in group A for scalp acupuncture treatment. The scores of Gross Motor Function Measure (GMFM) were observed before and after treatment for children. The clinical efficacy of each group was evaluated. By follow-up for 12 months, the condition of independent walking was observed. The total effective rate in group A was 79.6% (39/49), which was superior to that of group B [49.0% (24/49)] and group C [51.0% (25/ 49)] respectively (both P < 0.05). After treatment, GMFM scores of children were all improved significantly in 3 groups (P < 0.001, P < 0.05), of which, the improvement extent in group A was superior to that of other two groups (both P < 0.05). It was found after follow-up for 1 year that 31 cases could walk independently in group A, which was more than group B (17 cases) and group C (16 cases). The quick meridian needling therapy plus scalp acupuncture can improve significantly limb motor function of children with cerebral palsy and its therapeutic effect is superior to conventional acupuncture and simple scalp acupuncture.

Zhang Q.-H.,Peoples Hospital of Sichuan Province
International Eye Science | Year: 2016

AIM: To observe the effect of Nd: YAG laser on vitreous floaters. METHODS: According to the criteria of the study, the 35 eyes of 35 patients with floaters determined by slitlamp with front mirror and type B ultrasound scan were enrolled. The Nd: YAG laser was used to destroy the vitreous floaters in order to make them easier to be absorbed. The changes of vitreous floaters, visual acuity, IOP, the results of slitlamp examination, type B ultrasound scan and complications were observed before and after the treatments several times. RESULTS: There were 11 eyes with high or moderate myopia in the 35 eyes. In all patients, the spotted vision improved respectively 2h after the laser procedure. After Nd: YAG laser treatment, the spotted vision disappeared in 3 eyes (9%)within 24h, and in 11 eyes(31%) within 7d, and in 27 eyes(77%) within 30 d, and the effective rate was 77% after 30d. The second laser treatment performed 7d after the first laser treatment in 2 eyes(6%). There was no significant difference on efficacy between patients with low myopia and with high or moderate myopia. The treatment effect in patients with low myopia was better than that in patients with moderate or high myopia 30d after treatments(P<0.05). No complication occured in or after the treatments. CONCLUSION: The Nd: YAG laser can destroy the vitreous floaters, make them easier to be absorbed and obviously improve the visual acuity, which is a effective way to treat vitreous floaters. Careful case selection may decrease the complication risk. Copyright 2016 by the IJO Press.

Li Z.,University of Sichuan | Min W.,Peoples Hospital of Sichuan Province | Gou J.,University of Sichuan
Cancer Chemotherapy and Pharmacology | Year: 2013

Purpose: Paclitaxel resistance remains to be a major obstacle to the chemotherapy of endometrial cancer. Using proteomic-based approach, we used to identify cyclophilin A (CypA) as a potential therapeutic target for endometrial cancer. As a natural continuation, this study aimed to reveal the correlation between CypA and paclitaxel resistance and evaluate the possibility of CypA as a therapeutic target for reversal of resistance. Methods: Two paclitaxel-resistant endometrial cancer cell sublines HEC-1-B/TAX and AN3CA/TAX were generated, and expressions of CypA, P-gp, MRP-2 and survivin were demonstrated by Western blotting. CypA was knocked down by RNA interference, and the subsequent effects on the alteration of paclitaxel resistance were examined by MTT, flow cytometry and migratory/invasive transwell assays. MAPK kinases activities were examined by Western blotting. Results: CypA knockdown led to significant inhibition of cell proliferation, induction of apoptosis and suppression of migratory/invasive capacity in HEC-1-B/TAX and AN3CA/TAX cells when exposed to paclitaxel. CypA knockdown led to reductions in total and phosphorylated MAPK kinases, including Akt, ERK1/2, p38 MAPK and JNK, in HEC-1-B/TAX cells. Furthermore, pretreatment with MAPK kinase inhibitors exhibited a synergistic effect in combination with CypA knockdown. Conclusions: These results demonstrated that CypA expression was up-regulated in paclitaxel-resistant cancer cells, and knockdown of CypA could reverse the paclitaxel resistance through, at least partly, suppression of MAPK kinase pathways, presenting a possibility of CypA serving as a therapeutic target to overcome paclitaxel resistance. © 2013 Springer-Verlag Berlin Heidelberg.

Yu J.,Tianjin Medical University | Zhao C.,Tianjin Medical University | Zhao C.,Peoples Hospital of Sichuan Province | Luo X.,Xiangyang First Peoples Hospital
Anesthesia and Analgesia | Year: 2013

BACKGROUND:: Electroacupuncture (EA), as a traditional clinical method, is widely accepted in pain clinics, but the analgesic effect of EA has not been fully demonstrated. In the present study, we investigated the effect of EA on chronic pain and expression of P2X3 receptors in the spinal cord of rats with chronic constriction injury (CCI). METHODS:: The study was conducted in 2 parts. In part 1, Sprague Dawley rats were divided into 6 groups (n = 10): sham-CCI, CCI, LEA; CCI + 2 Hz EA at acupoints), HEA; CCI + 15 Hz EA at acupoints), NA-LEA (CCI + 2 Hz EA at nonacupoints), and NA-HEA (CCI + 15 Hz EA at nonacupoints). EA treatment was performed once a day on days 4 to 9 after CCI. Nociception was assessed using von Frey filaments and a hotplate apparatus. The protein and the messenger RNA (mRNA) levels of P2X3 receptors in the spinal cord were assayed by Western blotting and real-time polymerase chain reaction, respectively. In part 2, rats were divided into 5 groups (n = 10): sham-CCI, CCI, EA (CCI + EA at acupoints), NA-EA (CCI + EA at nonacupoints), and U0126 (CCI + intrathecal injection of U0126). EA treatment was conducted similar to part 1. Rats were given 5 μg U0126 in the U0126 group and 5% dimethyl sulfoxide intrathecally. Ten microliters was used as a vehicle for the other 4 groups twice a day on days 4 to 9 after CCI. Extracellular signal-regulated kinase 1/2 (ERK1/2) and ERK1/2 phosphorylation in the spinal cord were also assayed by Western blotting. RESULTS:: EA treatment exhibited significant antinociceptive effects and reduced the CCI-induced increase of both protein and mRNA expression of P2X3 receptors in the spinal cord. Furthermore, 2 Hz EA had a better analgesic effect than 15 Hz EA, and the protein and mRNA level of P2X3 receptor in spinal cord were lower in rats treated with 2 Hz EA at acupoints than 15 Hz EA at acupoints. Either EA at acupoints or intrathecal injection of U0126 relieved allodynia and hyperalgesia and reduced the expression of P2X3 receptors and ERK1/2 phosphorylation in the spinal cord. CONCLUSIONS:: The data demonstrated that EA alleviates neuropathic pain behavior, at least in part, by reducing P2X3 receptor expression in spinal cord via the ERK1/2 signaling pathway. Low frequency EA has a better analgesic effect than high frequency HEA on neuropathic pain. Copyright © 2012 International Anesthesia Research Society.

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