Liu P.,Zaozhuang Municipal Hospital |
Man Y.,Zaozhuang Municipal Hospital |
Wang Y.,Peoples Hospital of Shizhong District |
Bao Y.,Zaozhuang Municipal Hospital
Oncology Letters | Year: 2016
Bone morphogenetic protein 9 (BMP9), also known as growth differentiation factor 2, plays a key role in promoting osteosarcoma growth. However, the underlying mechanism remains to be determined. The aim of the present study was to determine the mechanism of BMP9 promoting the growth of osteosarcoma mediated by the Notch signaling pathway. Osteosarcoma cell lines, 143B and MG63, were used for the in vitro experiments. Cell proliferation, cell migration and cell cycle transformation were monitored under various settings. The control and experimental groups used in the present study were BMP9 adenovirus (AdBMP9), a recombinant adenovirus expressing the dominant-negative mutant of Notch1 (AdR-dnNotch1), AdBMP9+AdR-dnNotch1 and AdBMP9+compound E (blocker of the Notch signaling pathway). The results showed that Notch ligands DLL1, JAG1 and JAG2, as well as Notch receptors Notch1, Notch2 and Notch3 were markedly expressed in the two cell lines. Cell proliferation and migration ability increased in the AdBMP9 group and were higher than that in the AdBMP9+AdR-dn Notch1 and AdBMP9+compound E group. Cell proliferation and migration in the AdR-dnNothc1 group was lower than that in the AdBMP9 group, although the differences were not statistically significant (P>0.05). The cell cycle ratio in the S/G2 phase increased significantly in the AdBMP9 group and was higher than that in the AdBMP9+AdR-dnNotch1 and AdBMP9+compound E groups. By contrast, the ratio of the cell cycle in S/G2 phase in the AdR-dnNotch1 group was lower than that in the AdBMP9 group. The differences were not statistically significant (P>0.05). In conclusion, the results showed that the Notch signaling pathway plays an important role in mediating the growth of osteosarcoma promoted by BMP9. © 2016, Spandidos Publications. All rights reserved.
Gao J.,Weifang Medical University |
Wang M.-G.,Jinan Central Hospital |
Yang S.,Weifang Medical University |
Li X.,Weifang Medical University |
And 3 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: Bone deficiency restricts the extensive use of oral implant restoration. How to promote stem cell migration, adhesion and proliferation so as to promote endogenous bone regeneration becomes the key of research. OBJECTIVE: To observe the effects of different growth factors on the migration, adhesion and proliferation of rabbit adipose tissue-derived stem cells cultured in vitro and to screen the optimal combined factors. METHODS: The inguinal adipose tissues of rabbits were cut under aseptic conditions and primary adipose tissue-derived stem cells were cultured by enzyme digestion method. Passage 3 cells were randomly divided into five groups: transforming growth factor β1+platelet-derived growth factor AB group (group 1), platelet-derived growth factor AB+vascular endothelial growth factor group (group 2), transforming growth factor βi +vascular endothelial growth factor group (group 3), transforming growth factor βi +platelet-derived growth factor AB+vascular endothelial growth factor group (group 4), and blank control group. In vitro cell migration ability was examined by Transwell chamber. Cell proliferation and adhesion abilities were examined by cell counting kit-8 and cell adhesion assay, respectively. RESULTS AND CONCLUSION: Results from the Transwell test showed that the migration number of rabbit adipose tissue-derived stem cells showed significant differences among the former four groups (P < 0.05), which was highest in the group 4 that significantly promoted the migration of adipose tissue-derived stem cells. In the cell adhesion assay, there were significant differences in the number of adherent cells among the former four groups (P < 0.05), and the group 3 showed the most adherent cells that dramatically improved the adhesion of adipose tissue-derived stem cells. Compared with the blank control group, the absorbance values in the groups 1 -4 were significantly higher at days 1, 3, 5, 7 (P < 0.05), and the group 2 showed the highest absorbance value, indicating the combination of platelet-derived growth factor AB and vascular endothelial growth factor significantly promoted the proliferation of rabbit adipose tissue-derived stem cells.