Peoples Hospital Of Shizhong District

Jinan, China

Peoples Hospital Of Shizhong District

Jinan, China
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Peng J.,Leshan Vocational and Technical College | Chen X.,Peoples Hospital Of Shizhong District | Wang A.-P.,Leshan Hospital of Traditional Chinese Medicine | Luo L.,Leshan Hospital of Traditional Chinese Medicine | And 2 more authors.
Journal of Acupuncture and Tuina Science | Year: 2017

Objective: To observe the clinical efficacy of electroacupuncture (EA) on Alzheimer’s disease (AD). Methods: A total of 50 AD patients were randomly allocated into a Western medication (WM) group (n=25) and an acupuncture plus medication (APM) group (n=25). Patients in the WM group took oral huperzine A capsules. In addition to huperzine A capsules, patients in the APM group also received EA at Shenting (GV 24), Baihui (GV 20), Dazhui (GV 14), Fengfu (GV 16), Mingmen (GV 4) and Yongquan (KI 1). The needles on the above points were connected to G6805-II electric stimulator [3 pairs: Shenting (GV 24) and Baihui (GV 20); Dazhui (GV 14) and Fengfu (GV 16); and bilateral Yongquan (KI 1)]. The needles were retained 25 min. The treatment was done once a day, and 10 times made up a course of treatment. The patients received a total of 3 treatment courses. There was a 3-day interval between two courses. The mini-mental state examination (MMSE) and Hasegawa dementia scale revised (HDS-R) were conducted before and after treatment. The clinical efficacies were evaluated when the treatment was completed. Results: Before treatment, there were no between-group statistical differences in MMSE and HDS-R scores (both P>0.05). After treatment, the MMSE and HDS-R scores in the APM group were significantly higher than those in the WM group (both P<0.05). The total effective rate in the APM group was 88.0%, versus 76.0% in the WM group, showing a statistical difference (P<0.05). Conclusion: EA is effective for AD and can improve clinical symptoms in AD patients. © 2017, Shanghai Research Institute of Acupuncture and Meridian and Springer-Verlag GmbH Germany.


Gao J.,Weifang Medical University | Wang M.-G.,Jinan Central Hospital | Yang S.,Weifang Medical University | Li X.,Weifang Medical University | And 3 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Bone deficiency restricts the extensive use of oral implant restoration. How to promote stem cell migration, adhesion and proliferation so as to promote endogenous bone regeneration becomes the key of research. OBJECTIVE: To observe the effects of different growth factors on the migration, adhesion and proliferation of rabbit adipose tissue-derived stem cells cultured in vitro and to screen the optimal combined factors. METHODS: The inguinal adipose tissues of rabbits were cut under aseptic conditions and primary adipose tissue-derived stem cells were cultured by enzyme digestion method. Passage 3 cells were randomly divided into five groups: transforming growth factor β1+platelet-derived growth factor AB group (group 1), platelet-derived growth factor AB+vascular endothelial growth factor group (group 2), transforming growth factor βi +vascular endothelial growth factor group (group 3), transforming growth factor βi +platelet-derived growth factor AB+vascular endothelial growth factor group (group 4), and blank control group. In vitro cell migration ability was examined by Transwell chamber. Cell proliferation and adhesion abilities were examined by cell counting kit-8 and cell adhesion assay, respectively. RESULTS AND CONCLUSION: Results from the Transwell test showed that the migration number of rabbit adipose tissue-derived stem cells showed significant differences among the former four groups (P < 0.05), which was highest in the group 4 that significantly promoted the migration of adipose tissue-derived stem cells. In the cell adhesion assay, there were significant differences in the number of adherent cells among the former four groups (P < 0.05), and the group 3 showed the most adherent cells that dramatically improved the adhesion of adipose tissue-derived stem cells. Compared with the blank control group, the absorbance values in the groups 1 -4 were significantly higher at days 1, 3, 5, 7 (P < 0.05), and the group 2 showed the highest absorbance value, indicating the combination of platelet-derived growth factor AB and vascular endothelial growth factor significantly promoted the proliferation of rabbit adipose tissue-derived stem cells.


Liu P.,Zaozhuang Municipal Hospital | Man Y.,Zaozhuang Municipal Hospital | Wang Y.,Peoples Hospital of Shizhong District | Bao Y.,Zaozhuang Municipal Hospital
Oncology Letters | Year: 2016

Bone morphogenetic protein 9 (BMP9), also known as growth differentiation factor 2, plays a key role in promoting osteosarcoma growth. However, the underlying mechanism remains to be determined. The aim of the present study was to determine the mechanism of BMP9 promoting the growth of osteosarcoma mediated by the Notch signaling pathway. Osteosarcoma cell lines, 143B and MG63, were used for the in vitro experiments. Cell proliferation, cell migration and cell cycle transformation were monitored under various settings. The control and experimental groups used in the present study were BMP9 adenovirus (AdBMP9), a recombinant adenovirus expressing the dominant-negative mutant of Notch1 (AdR-dnNotch1), AdBMP9+AdR-dnNotch1 and AdBMP9+compound E (blocker of the Notch signaling pathway). The results showed that Notch ligands DLL1, JAG1 and JAG2, as well as Notch receptors Notch1, Notch2 and Notch3 were markedly expressed in the two cell lines. Cell proliferation and migration ability increased in the AdBMP9 group and were higher than that in the AdBMP9+AdR-dn Notch1 and AdBMP9+compound E group. Cell proliferation and migration in the AdR-dnNothc1 group was lower than that in the AdBMP9 group, although the differences were not statistically significant (P>0.05). The cell cycle ratio in the S/G2 phase increased significantly in the AdBMP9 group and was higher than that in the AdBMP9+AdR-dnNotch1 and AdBMP9+compound E groups. By contrast, the ratio of the cell cycle in S/G2 phase in the AdR-dnNotch1 group was lower than that in the AdBMP9 group. The differences were not statistically significant (P>0.05). In conclusion, the results showed that the Notch signaling pathway plays an important role in mediating the growth of osteosarcoma promoted by BMP9. © 2016, Spandidos Publications. All rights reserved.


PubMed | Peoples Hospital of Shizhong District and Zaozhuang Municipal Hospital
Type: Journal Article | Journal: Oncology letters | Year: 2016

Bone morphogenetic protein 9 (BMP9), also known as growth differentiation factor 2, plays a key role in promoting osteosarcoma growth. However, the underlying mechanism remains to be determined. The aim of the present study was to determine the mechanism of BMP9 promoting the growth of osteosarcoma mediated by the Notch signaling pathway. Osteosarcoma cell lines, 143B and MG63, were used for the

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