Peoples Hospital of Shenzhen

of Shenzhen, China

Peoples Hospital of Shenzhen

of Shenzhen, China
SEARCH FILTERS
Time filter
Source Type

Ling D.,Southern Medical University | Ling D.,Peoples Hospital Of Shenzhen | Wang F.,Liuzhou Peoples Hospital | Li P.,Longgang District Peoples Hospital Of Shenzhen | Xu S.,Southern Medical University
International Journal of Clinical and Experimental Medicine | Year: 2017

Objective: To investigate the effects of EphB1 inhibitor (EphB1-Fc) on tibial cancer pain in rats by intrathecal injection and its related mechanism. Methods: Thirty two male SD rats successfully underwent sheath built-in tube were assigned into 4 groups (n=8) according to random number table: sham operation group (C group, as normal control group), model group (BCP group), model + solvent group (BCP + DMSO group), model + EphB1-Fc group (BCP + E group). A model of bone cancer pain (BCP) was established by injecting Walker256 rat breast cancer cells into the medullary cavity of left tibial. Intramuscular administration was performed 1 day after BCP modeling, once a day for 14 days. The rats in BCP + DMSO and BCP + E groups were respectively injected intrathecally with 20 µl 10% DMSO and 10 µg EphB1-Fc for 20 µl. The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) of rats’ left hindlimb were measured at 1 day before operation and 1, 3, 5, 7 and 14 days after operation. On the 14th day after operation, Western blot was adopted to detect phosphorylation of JNK and activation of NF-κB p65 in the rat’s spinal dorsal horn of each group. Results: Praxiology: compared with C group, the MWT and TWL of BCP group decreased significantly from 5th to 14th day after operation. Compared with BCP group 1 day before operation, the MWT and TWL of that showed a progressive reduction from 5th to 14th day after operation, and it reached the maximum level on the 14th day after the inoculation of tumor cells. Thermal and mechanical hyperalgesia caused by tumor cell implantation were significantly inhibited from 5th to 14th day after operation through successive intrathecal injection of EphB1-Fc, an inhibitor of EphB1 receptor signaling pathway; meanwhile, repeated use of EphB1-Fc significantly inhibited the activation of NF-κB p65 protein (P=0.02) and the phosphorylation of JNK protein (P=0.01) induced by BCP. Conclusion: EphB1-Fc can block the activation of JNK and NF-κB p65 pathway by inhibiting the binding of EphB1 and its receptor, thus alleviating the tibia cancer pain in rats. © 2017, E-Century Publishing Corporation. All rights reserved.


Ren J.,Jinan University | Qin B.,Shenzhen University | Zou C.,Peoples Hospital of Shenzhen | He J.,Shenzhen University | Liu S.,Shenzhen University
Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology | Year: 2017

Background: Studies showed that rapamycin (Rapa) plays a protective effect on central nervous system by improving the aging of human brain tissue and ameliorating rat cerebral metabolism after ischemia.Optical nerve and retinal ganglion cells (RGCs) are central nerve, however, whether Rapa can protect hypoxia-injuried RGCs is still unclear. Objective: This study was to explore the protective role of Rapa on hypoxia RGC-5, a rat RGC line, and its underlying mechanism in order to provide a new strategy for the treatment of traumatic optic neuropathy. Methods: Rat RGC-5 cells were cultured using DMEM with 10% fetal bovine serum, and the cells were observed under the inverted phase contrast microscope.The cells were treated by 50, 100, 200, 400 and 600 μmol/L CoCl2for 24 hours and 48 hours, respectively, and Clone Select Imager was employed to assess the survival rate.The CoCl2-induced hypoxia cell models were established by adding 200 μmol/L CoCl2 in the medium for 24 hours, and then the 0.1, 0.4, 1.6, 6.4 μmol/L Rapa was used to treat the models for 24 hours in the Rapa intervention group, respectively.The cells cultured by DMEM with 10% fetal bovine serum served as the normal control group.The survival rate of the cells was evaluated by Clone Select Imager; the apoptotic rate of the cells was assayed by AnnexinV-FITC/PI double-staining flow cytometry; JC-1 probe was used to detect the mitochondrial trans-membrane potential, and the expression of bax mRNA in the cells was detected by real-time fluorescence quantitative PCR. Results: The survival rate of the cells was (70.51±5.00)% in the 200 μmol/L CoCl2-treated group, which was significantly lower than (100.00±3.29)% in the normal control group (P<0.01). The survival rate of the cells was significantly different among the normal control group and 0.1, 0.4, 1.6, 6.4 μmol/L Rapa intervention groups (F=167.904, P=0.000), and the survival rate was evidently higher in the 0.1 μmol/L Rapa intervention group than that in the model control group (P<0.05). The apoptotic rate was 25.4%, 37.7% and 25.3%, while mitochondrial trans-membrane potential reduced by 0.4%, 6.3% and 1.4% in the normal control group, model control group and 0.1 μmol/L Rapa intervention group, respectively.The relative expression of bax mRNA in the cells was 1.01±0.21, 3.52±0.30 and 1.66±0.20 in the normal control group, model control group and 0.1 μmol/L Rapa intervention group, showing a significant difference among the groups (F=88.034, P=0.000), and the relative expression of bax mRNA in the model control group was considerably elavated in comparison with the normal control group and 0.1 μmol/L Rapa intervention group (both at P<0.05). Conclusions: Rapa protects the RGC-5 cells against CoCl2-induced hypoxic damage primarily by down-regulating the expression of bax in the cells and improving the survival rate of RGC-5 cells in vitro. Copyright © 2017 by the Chinese Medical Association.


Wang F.,Southern Medical University | Wang F.,Peoples Hospital Of Liuzhou | Ling D.,Peoples Hospital Of Shenzhen | Xu S.,Southern Medical University
International Journal of Clinical and Experimental Medicine | Year: 2017

Objective: To investigate the effect of ketamine on the PKA/CREB signalling pathway in early developing mice and to explore the molecular mechanism of ketamine on the learning and memory ability impairment. Methods: Sixty Kunming mice weighing 18-25 g were randomly divided into two experimental groups, with 30 mice in each group. These 30 mice were then randomly subdivided into three subgroups (ketamine group, vehicle group and control group). Mice in the ketamine group underwent intraperitoneal injection of ketamine (50 mg/kg), whereas mice in the vehicle group were given an intraperitoneal injection of the same volume of normal saline. And these two groups both received these injections once daily for six consecutive days. However, mice in the control group received no injections. On the seventh day of the experiment, the learning ability of mice was measured via a step-down test and Morris water maze test. Hippocampus proteins were then extracted from the mice, and the activation and expression of proteins involved in the PKA/CREB signalling pathway were detected using Western Blot technology. Results: Inthe ketamine group, the latency period was shortened, error times were increased, the escape latency was prolonged, the expression of PKAcα was decreased and phosphorylated-CREB was reduced, as compared with the corresponding measures in the control group. Conclusion: Ketamine can impair the learning and memory ability in early developing mice, and its mechanism may be related to the inhibition of the hippocampal PKA/CREB signalling pathway. © 2017, E-Century Publishing Corporation. All rights reserved.


PubMed | Peoples Hospital of Shenzhen, Centers for Disease Control and Prevention, LongHua new District Center for Disease Control and Prevention and Huazhong University of Science and Technology
Type: Journal Article | Journal: PloS one | Year: 2015

microRNA (miRNA) plays a role in the pathogenesis of ischemic stroke, and single nucleotide polymorphisms in miRNA genes may contribute to disease susceptibility. However, the effect of miR-146a, miR-196a2, and miR-499 polymorphisms on ischemic stroke susceptibility has been rarely reported. Using the TaqMan assay, we evaluated the association of hsa-miR-146a/rs2910164, hsa-miR-196a2/rs11614913, and hsa-miR-499/rs3746444 polymorphisms with the risk of ischemic stroke in a Chinese population with 531 ischemic stroke patients and 531 control subjects. Rs2910164 C/G genotypes were significantly associated with increased risk of ischemic stroke in different genetic model (homozygote comparison: OR = 2.00, 95% CI, 1.29-3.12, P = 0.002; additive model: OR = 1.35, 95% CI, 1.10-1.65, P = 0.004;dominant model: OR = 1.33, 95% CI, 1.00-1.75, P = 0.049; recessive model: OR = 1.82, 95% CI, 1.20-2.74, P = 0.004). Subjects with allele G of hsa-miR-146a/ rs2910164 also showed increased risk of ischemic stroke (OR = 1.33, 95% CI, 1.09-1.62, P = 0.005). Stratification analysis showed that the association between rs2910164 and the risk of ischemic stroke was more pronounced in subjects over 60 years old, females, non-drinkers, subjects without hypertension or diabetes mellitus. There were significant combined effects between miR-146a/rs2910164 and fasting glucose/low-density lipoprotein cholesterol levels on ischemic stroke susceptibility. However, we failed to find any association between the alleles/genotypes of rs11614913 T/C and ischemic stroke, respectively (P> 0.05). In summary, this study provides evidence that miR-146a/rs2910164 might be associated with a significantly increased risk of ischemic stroke in a Chinese population, and the combined effects between miRNA polymorphism and fasting glucose /blood lipid levels may contribute to stroke pathogenesis.


Huang S.,Centers for Disease Control and Prevention | Zhou S.,LongHua New District Center for Disease Control and Prevention | Zhang Y.,Centers for Disease Control and Prevention | Lv Z.,Centers for Disease Control and Prevention | And 11 more authors.
PLoS ONE | Year: 2015

microRNA (miRNA) plays a role in the pathogenesis of ischemic stroke, and single nucleotide polymorphisms in miRNA genes may contribute to disease susceptibility. However, the effect of miR-146a, miR-196a2, and miR-499 polymorphisms on ischemic stroke susceptibility has been rarely reported. Using the TaqMan assay, we evaluated the association of hsa-miR-146a/rs2910164, hsa-miR-196a2/rs11614913, and hsa-miR-499/rs3746444 polymorphisms with the risk of ischemic stroke in a Chinese population with 531 ischemic stroke patients and 531 control subjects. Rs2910164 C/G genotypes were significantly associated with increased risk of ischemic stroke in different genetic model (homozygote comparison: OR = 2.00, 95% CI, 1.29-3.12, P = 0.002; additive model: OR = 1.35, 95% CI, 1.10-1.65, P = 0.004;dominant model: OR = 1.33, 95% CI, 1.00-1.75, P = 0.049; recessive model: OR = 1.82, 95%CI, 1.20-2.74, P = 0.004). Subjects with allele G of hsa-miR-146a/ rs2910164 also showed increased risk of ischemic stroke (OR = 1.33, 95%CI, 1.09-1.62, P = 0.005). Stratification analysis showed that the association between rs2910164 and the risk of ischemic stroke was more pronounced in subjects over 60 years old, females, non-drinkers, subjects without hypertension or diabetesmellitus. There were significant combined effects between miR-146a/rs2910164 and fasting glucose/low-density lipoprotein cholesterol levels on ischemic stroke susceptibility. However, we failed to find any association between the alleles/genotypes of rs11614913 T/C and ischemic stroke, respectively (P> 0.05). In summary, this study provides evidence that miR-146a/rs2910164 might be associated with a significantly increased risk of ischemic stroke in a Chinese population, and the combined effects between miRNA polymorphism and fasting glucose /blood lipid levels may contribute to stroke pathogenesis. © 2015 Huang et al.


Wang C.-M.,Peoples Hospital of Shenzhen | Ling Z.-G.,Guangxi Medical University | Wu Y.-B.,Guangxi Medical University | Cai S.-Q.,Guangxi Medical University | And 3 more authors.
PLoS ONE | Year: 2016

Objective Pleural lavage cytology (PLC) is considered as a possible tool for assessing prognosis of lung cancer patients. We aimed to comprehensively review the prognosis value of PLC in patients undergoing surgical resection. Methods We searched 4 electronic databases for relevant studies comparing positive PLC and negative PLC. The primary outcomes included survival rate and recurrence rate at maximum follow-up. Results The meta-analysis included 28 studies, with a total of 20,714 patients. For the overall survival rate of all stages, the results demonstrated that positive pre-resection, post-resection and pooled PLC were associated with unfavorable survival: hazard ratio (HR) 2.89 (95% confidence interval [CI] 2.48-3.37), 2.70 (1.90-3.83), and 2.89 (2.52-3.31), respectively. For the stage I survival rate, the combined results also suggested that positive pre-resection, post-resection and pooled PLC were associated with unfavorable survival: HR 3.29 (95% CI 2.55-4.25), 4.85 (2.31-10.20), and 3.16 (2.53-3.94), respectively. Furthermore, a meta-analysis of 14 studies included 14,279 patients showed that positive pre-resection, post-resection and pooled PLC were associated with an increased risk of overall recurrence: risk ratio (RR) 2.45 (95% CI 1.91-3.15), 2.37 (1.11-5.09), and 2.37 (95% CI 2.00-2.80), respectively. Positive PLC was also associated with a high pleural recurrence (RR 4.77; 95% CI 3.13-7.26) or distant recurrence (RR 2.33; 95% CI 1.65-3.29). Conclusions Both positive pre- resection and post-resection PLC are associated with not only higher tumor recurrence but also unfavorable survival outcomes in patients with lung cancer resection. This technique can therefore act as a strong prognostic factor for tumor recurrence and adverse survival rates. © 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Wang S.-J.,Shandong University | Zhao X.-H.,Shandong University | Chen W.,Shandong University | Bo N.,Peoples Hospital of Shenzhen | And 3 more authors.
Molecular Medicine Reports | Year: 2015

Sirtuin 1 (SIRT1) regulates numerous neuronal processes, including metabolism, antioxidation and aging, through activation of peroxisome proliferator-activated receptor coactivator 1-α (PGC-1α), an upstream regulator of mitochondrial biogenesis and function. However, the role of SIRT1 in the oxidative stress induced by seizures has yet to be elucidated. The present study aimed to investigate whether SIRT1 was involved in the activation of the PGC-1α/mitochondrial antioxidant system following status epilepticus (SE) in rats. The data demonstrated that SIRT1 expression and activity were enhanced in the rat hippocampus following SE. SIRT1 inhibition effectively blocked the SE-associated increase in PGC-1α and mitochondrial antioxidant enzymes, including superoxide dismutase 2 (SOD2) and uncoupling protein 2 (UCP2). Additionally, it was also demonstrated that the activation of SIRT1 enhanced mitochondrial electron transport chain complex I activity and increased ATP content. In conclusion, the present results suggest that SIRT1 activation may alleviate mitochondrial oxidative stress induced by seizures partially via PGC-1α signaling.


PubMed | Peoples Hospital of Shenzhen, Shandong Ankang Hospital and Shandong University
Type: Journal Article | Journal: Molecular medicine reports | Year: 2014

Sirtuin 1 (SIRT1) regulates numerous neuronal processes, including metabolism, antioxidation and aging, through activation of peroxisome proliferator-activated receptor coactivator 1- (PGC-1), an upstream regulator of mitochondrial biogenesis and function. However, the role of SIRT1 in the oxidative stress induced by seizures has yet to be elucidated. The present study aimed to investigate whether SIRT1 was involved in the activation of the PGC-1/mitochondrial antioxidant system following status epilepticus (SE) in rats. The data demonstrated that SIRT1 expression and activity were enhanced in the rat hippocampus following SE. SIRT1 inhibition effectively blocked the SE-associated increase in PGC-1 and mitochondrial antioxidant enzymes, including superoxide dismutase 2 (SOD2) and uncoupling protein 2 (UCP2). Additionally, it was also demonstrated that the activation of SIRT1 enhanced mitochondrial electron transport chain complex I activity and increased ATP content. In conclusion, the present results suggest that SIRT1 activation may alleviate mitochondrial oxidative stress induced by seizures partially via PGC-1 signaling.


Guo Y.,Peoples Hospital of Shenzhen | Zhang Y.,Peoples Hospital of Shenzhen | Chen Z.,Peoples Hospital of Shenzhen | Xin Z.,Peoples Hospital of Shenzhen
Experimental and Therapeutic Medicine | Year: 2015

The aim of the present study was to compare the advantages and disadvantages of the combined application of recombinant human thyroid stimulating hormone (rhTSH) with thyroid hormone withdrawal (THW) and THW alone prior to 131I therapy for the treatment of differentiated thyroid cancer. Four indicators were compared between the experimental group, who received a combined therapeutic method of rhTSH with THW, and the control group, who received THW therapy alone. With the exception of the elimination half-time of 131I in the blood in the experimental group, which was significantly shorter compared with that in the control group, the other three indicators, including the urinary iodine concentration, the relative 131I uptake ratio of the neck lesions and the one-time cure rate, were not significantly different between the two groups. In addition, the treatment efficacy of 131I therapy exhibited no statistically significant difference between the experimental and control groups. However, in the experimental group, the residence time of 131I in the blood was significantly shorter compared with that in the control group, indicating that the irradiation damage of radioactive iodine exposure to the non-target tissues was lower in the experimental group when compared with the control group. In addition, no evident hypothyroidism was observed in the patients. Thus, the combined application of rhTSH with THW prior to 131I therapy was demonstrated to be superior to the THW therapy alone. © 2015 Spandidos Publications. All rights reserved.


He S.,Peoples Hospital of Shenzhen | Luo J.,Peoples Hospital of Shenzhen | Chen J.,Peoples Hospital of Shenzhen
Journal of Acupuncture and Tuina Science | Year: 2011

Objective: To observe the therapeutic effects of acupuncture in managing chronic gastritis. Methods: The subjects were 102 patients with chronic gastritis, receiving acupuncture at Jiaji (Ex-B 2) plus cupping for treatment. Results: Among the 102 patients, 31 were clinically recovered, 62 got improved, and the total effective rate was 91.2%. Conclusion: Acupuncture therapy is effective in treating chronic gastritis. © 2011 Shanghai Research Institute of Acupuncture and Meridian and Springer-Verlag Berlin Heidelberg.

Loading Peoples Hospital of Shenzhen collaborators
Loading Peoples Hospital of Shenzhen collaborators