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Zhang X.,Shenzhen Futian Hospital of TCM | Nie Y.,Guangzhou University | Li X.,Fourth Peoples Hospital of Shenzhen City Futian Hospital | Wu G.,Fourth Peoples Hospital of Shenzhen City Futian Hospital | And 9 more authors.
Pathology and Oncology Research | Year: 2014

Based on our previous experiments, this study is to further investigate the functional significance of miR-181a and its target gene in gastric cancer. Expression of miR-181a was detected by qRT-PCR in three normal gastric tissues and three human gastric cancer cell lines (SGC-7901, MGC-803, and BGC-823 cells). After transfection with miR-181a inhibitor, proliferation, apoptosis, migration, and invasion of the SGC-7901 cells were evaluated. Ataxia-telangiectasia mutation (ATM) was predicted as a target gene of miR-181a with bioinformatics analysis, and was verified by lucifersae reporter assay. Expression of ATM protein in HEK293T cells and tissues was measured by Western Blot. Expression of ATM mRNA in HEK293T cells was measured by RT-PCR. Compared with three non-tumour tissues, the expression of miR-181a in three gastric cancer cells was significantly increased by 26.68, 14.83 and 14.96 folds; Compared with Negative Control(NC) and blank groups, transfection of miR-181a inhibitor led to inhibition of SGC7901 cell proliferation, invasion, and migration as well as promotion of apoptosis. A luciferase reporter assay demonstrated that ATM was a direct target of miR-181a, miR-181a mimics transfection down regulated ATM mRNA and protein expression. There was inverse correlation between miR-181a and ATM protein expression in gastric cancer and normal gastric tissues. Our study demonstrates that over-expression of miR-181a might be involved in development of gastric cancer by promoting proliferation and inhibiting apoptosis probably through directly targeting ATM. miR-181a modulation may be a potential strategy for the development of miRNA-based therapy of gastric cancer. © 2014 Arányi Lajos Foundation.


Hu Y.-Y.,Hubei University of Medicine | Du X.-Y.,Peoples Hospital of New District Longhua Shenzhen | Zhan A.-L.,Central Hospital of Shanghai Songjiang District | Zhou L.,Hubei University of Medicine | And 3 more authors.
Oncotarget | Year: 2016

Polymorphisms in the vascular endothelial growth factor (VEGF) gene may contribute to osteosarcoma risk, but the results of previous studies have been inconsistent and inconclusive. We conducted a meta-analysis to assess this association more accurately. Relevant studies were collected systemically from three online English databases. Crude odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the strength of the associations of three VEGF gene polymorphisms (+936C/T, -634 G/C, +1612 G/A) with osteosarcoma risk. Seven case-control studies involving 1,350 cases and 1,706 controls were selected for the meta-analysis. The pooled OR indicated that the VEGF +936C/T polymorphism was associated with increased risk of osteosarcoma in a Chinese population (T vs. C: OR = 1.26, 95% CI = 1.12-1.42, P < 0.01; TT vs. CC: OR = 1.70, 95% CI = 1.29-2.24, P < 0.01; CT + TT vs. CC: OR = 1.23, 95% CI = 1.06-1.44, P < 0.01; TT vs. CC + CT: OR = 1.61, 95% CI = 1.23-2.10, P < 0.01). A significant association was also found between the -634 G/C polymorphism and osteosarcoma risk (C vs. G: OR = 0.81, 95% CI = 0.69-0.96, P = 0.01; CC vs. GG: OR = 0.66, 95% CI = 0.48-0.90, P < 0.01; GC + CC vs. GG: OR = 0.80, 95% CI = 0.67-0.96, P = 0.02; CC vs. GG + GC: OR = 0.72, 95% CI = 0.60-0.86, P < 0.01). In sum, our meta-analysis suggests VEGF polymorphisms are associated with osteosarcoma susceptibility in the Chinese population. However, further studies that include different ethnicities and larger populations are needed.


PubMed | Hubei University of Medicine, Central Hospital of Shanghai Songjiang District, Peoples Hospital of New District Longhua Shenzhen and Nanjing Medical University
Type: Journal Article | Journal: Oncotarget | Year: 2016

Polymorphisms in the vascular endothelial growth factor (VEGF) gene may contribute to osteosarcoma risk, but the results of previous studies have been inconsistent and inconclusive. We conducted a meta-analysis to assess this association more accurately. Relevant studies were collected systemically from three online English databases. Crude odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the strength of the associations of three VEGF gene polymorphisms (+936C/T, -634 G/C, +1612 G/A) with osteosarcoma risk. Seven case-control studies involving 1,350 cases and 1,706 controls were selected for the meta-analysis. The pooled OR indicated that the VEGF +936C/T polymorphism was associated with increased risk of osteosarcoma in a Chinese population (T vs. C: OR = 1.26, 95% CI = 1.12-1.42, P < 0.01; TT vs. CC: OR = 1.70, 95% CI = 1.29-2.24, P < 0.01; CT + TT vs. CC: OR = 1.23, 95% CI = 1.06-1.44, P < 0.01; TT vs. CC + CT: OR = 1.61, 95% CI = 1.23-2.10, P < 0.01). A significant association was also found between the -634 G/C polymorphism and osteosarcoma risk (C vs. G: OR = 0.81, 95% CI = 0.69-0.96, P = 0.01; CC vs. GG: OR = 0.66, 95% CI = 0.48-0.90, P < 0.01; GC + CC vs. GG: OR = 0.80, 95% CI = 0.67-0.96, P = 0.02; CC vs. GG + GC: OR = 0.72, 95% CI = 0.60-0.86, P < 0.01). In sum, our meta-analysis suggests VEGF polymorphisms are associated with osteosarcoma susceptibility in the Chinese population. However, further studies that include different ethnicities and larger populations are needed.


PubMed | Peoples Hospital of New District Longhua Shenzhen and Wuhan University
Type: Journal Article | Journal: International journal of clinical and experimental medicine | Year: 2015

Interleukin-10 (IL-10) is likely to be closely correlated with the outbreak and progression of cancers though aiding tumors to free from the immune response. In previous studies, several polymorphisms sites including -1082A/G, -592A/C and -819T/C in the promoter region of IL-10 gene were proved to be involved in oral cancer. The purpose of this study was to further explore this association via a meta-analysis. There were four publications with 3783 cases and 4245 controls retrieved though electronic databases. The association among three IL-10 polymorphisms sites was estimated by summary odds ratios (ORs) and 95% confidence intervals (95% CIs) which were calculated using fixed-effect model. Subgroup analysis by ethnicity (Asian or Caucasian) was also performed for the analysis of IL-10-1082A/G polymorphism (three studies in Asians and one study in Caucasian). As a result, we found a moderately increased risk which was related to IL-10-1082A/G polymorphism in oral cancer under all the five contrasts [GG vs. AA: OR (95% CI)=2.95 (1.94-4.48); GG+AG vs. AA: OR (95% CI)=1.59 (1.35-1.86); GG vs. AG+AA: OR (95% CI)=2.59 (1.71-3.94); Allele G vs. Allele A: OR (95% CI)=1.68 (1.46-1.94); AG vs. AA: OR (95% CI)=1.53 (1.29-1.81)]. Additionally, the increased risk of oral cancer was also observed in Asians and Caucasians. However, the pooled data indicated that IL-10 -592A/C and -819T/C polymorphisms sites had no relationship with oral cancer risk. Taken together, the IL-10-1082A/G polymorphism site may act as a risk factor in oral cancer, and this issue still needs to be further verified.


PubMed | Hubei University of Medicine, Wuhan University and Peoples Hospital of New District Longhua Shenzhen
Type: | Journal: Scientific reports | Year: 2015

Molecular epidemiological research suggests that interleukin-10 (IL-10) polymorphisms may be associated with an increased risk of head and neck cancer (HNC), but results remain controversial. To derive a more precise evaluation, we performed a meta-analysis focused on genetic polymorphisms of IL-10. PubMed, Embase, CNKI and Wanfang databases were searched for studies that examined the relationship between IL-10 polymorphisms or haplotypes and HNC risk. The odds ratio (OR) and 95% confidence interval (CI) were applied to assess the relationship strength. Publication bias, sensitivity and cumulative analyses were conducted to measure the robustness of our findings. Overall, nine related studies involving 2,258 patients and 2,887 control samples were analyzed. Significant associations between the IL-10-1082A>G polymorphism and HNC risk were observed (G vs. A: OR=1.56, 95% CI=1.27-1.92, P<0.01, I(2)=69.4%; AG vs. AA: OR=1.64, 95% CI=1.32-2.05, P<0.01, I(2)=55.6%; GG vs. AA: OR=2.24, 95% CI=1.69-2.97, P<0.01, I(2)=38.5%; AG+GG vs. AA: OR=1.70, 95% CI=1.36-2.14, P=0.02, I(2)=61.8%; GG vs. AA+AG: OR=1.89, 95% CI=1.23-2.90, P=0.01, I(2)=46.3%) in the total population, as well as in subgroup analysis. Moreover, increased HNC risks were also associated with the IL-10 -819T>C polymorphism and the GCC haplotype. In conclusion, our meta-analyses suggest that IL-10 polymorphisms, specifically the -1082A>G polymorphism, may be associated with increased risk of HNC development.


PubMed | Hubei University of Medicine, Chung Shan Medical University, Nanjing Medical University and Peoples Hospital of New District Longhua Shenzhen
Type: | Journal: Scientific reports | Year: 2015

Molecular epidemiological studies have showed a closer association between microRNA polymorphisms with and head and neck cancer (HNC) risk. But the results of these studies were inconsistent. We performed this meta-analysis to clarify the associations between microRNA polymorphisms and HNC risk. Four electronic databases (PubMed, Embase, CNKI, and Wanfang) were searched. Odds ratios (ORs) with 95% confidence interval (CIs) were calculated to assess the association between microRNA-146a rs2910164 G > C, microRNA-196a2 rs11614913 C > T, microRNA-149 rs2292832 C > T, microRNA-499 rs3746444 A > G polymorphisms and HNC risk. Heterogeneity, publication bias and sensitivity analysis were conducted to guarantee the statistical power. Overall, 11 selected articles involving 16100 subjects were included in this meta-analysis. Significantly increased risk between microRNA-146a rs2910164 G > C polymorphism and HNC risk were observed in Caucasian population (GC vs. GG: OR = 1.31, 95%CI = 1.01-1.68; GC + CC vs. GG: OR = 1.26, 95%CI = 1.02-1.57). For microRNA-196a2 rs11614913 C > T, similarly increased risk were also found in Asian population (T vs. C, OR = 1.14, 95%CI = 1.04-1.25; TT vs. CC, OR = 1.33, 95%CI = 1.09-1.61; CT + TT vs. CC OR = 1.32, 95%CI = 0.99-1.76; TT vs. CC + CT, OR = 1.14, 95%CI = 0.99-1.33). In addition, no significant association was detected between microRNA-149 rs2292832 C > T and microRNA-499 rs3746444 A > G polymorphism and HNC risk. This meta-analysis demonstrates that microRNA polymorphisms are associated with HNC development based on ethnicity diversity.


PubMed | Jinan University, Peoples Hospital of New District Longhua Shenzhen and Shantou University
Type: Journal Article | Journal: Environmental science and pollution research international | Year: 2016

The peak occurrence of Mycoplasma pneumoniae (M. pneumoniae) infections in childhood and haze episodes is concurrent. Together, the prevalence of macrolide-resistant M. pneumoniae varies among countries might also be related to the concentration of ambient fine particulate mass (aerodynamic diameter 2.5m, PM2.5). Numerous cohort studies have identified consistent associations between ambient PM2.5 and cardiorespiratory morbidity and mortality. PM2.5 is a carrier of the heavy metals. The relationship between PM2.5-associated metals and M. pneumoniae infections in childhood has been increasingly drawing public attention. First, we reviewed original articles and review papers in Pubmed and Web of Science regarding M. pneumoniae and PM2.5-associated metal and analyzed the structural basis of PM2.5-associated metal interaction with M. pneumoniae. Then, the possible mechanisms of action between them were conjectured. Mechanisms of oxidative stress induction and modulation of the host immune system and inflammatory responses via Toll-like receptors (TLRs) and/or the nuclear factor-kappa B (NF-B) pathway are postulated to be the result of PM2.5-associated metal complex interaction with M. pneumoniae. In addition, a heavy metal effect on M. pneumoniae-expressed community-acquired respiratory distress syndrome (CARDS) toxin, and activation of the aryl hydrocarbon receptor (AhR) and TLRs to induce the differentiation of T helper (Th) cells are also regarded as important reasons for the influence of the heavy metals on the severity of M. pneumoniae pneumonia and the initial onset and exacerbation of M. pneumoniae associated asthma. PM2.5-associated metals via complex mechanisms can exert a great impact on the host through interaction with M. pneumoniae.


Bai X.-S.,Peoples Hospital Of New District Longhua Shenzhen | Liu J.-H.,Peoples Hospital Of New District Longhua Shenzhen | Xiao S.-M.,Peoples Hospital Of New District Longhua Shenzhen
Experimental and Therapeutic Medicine | Year: 2014

Agranulocytosis is a rare and serious adverse effect of antithyroid drugs (ATD), in particular methimazole (MMI), and usually develops within 3 months following the start of uninterrupted ATD treatment. Agranulocytosis may also develop for the first time following interruption and subsequent resumption of the same ATD treatment. In this case report, a 27-year-old female, who was treated for thyrotoxicosis with MMI, developed agranulocytosis following the discontinuation of MMI treatment for four months. To the best of our knowledge, this is the first study to report this. The aim of this report is to increase the awareness of physicians of the onset of agranulocytosis when MMI is discontinued, and to demonstrate that MMI should be used with caution.


Su G.,Peoples Hospital of New District Longhua Shenzhen | Xiang Y.,Peoples Hospital of New District Longhua Shenzhen | He G.,Peoples Hospital of New District Longhua Shenzhen | Jiang C.,Peoples Hospital of New District Longhua Shenzhen | And 3 more authors.
Archives of Medical Research | Year: 2014

Background and Aims: The efficacy of bisphosphonates (BPs) in treating bone loss associated with cancer therapies has been demonstrated in completed studies and ongoing clinical trials. The aim of this study was to investigate the evidence for BP use in treatment of bone loss in postmenopausal, early breast cancer (EBC) patients scheduled to receive aromatase inhibitors (AI). Methods: A comprehensive search for relative articles published until December 2013 was performed. The outcomes included the percentage and absolute change in lumbar spine (LS) and total hip (TH) bone mineral density (BMD). Before pooled meta-analysis, the studies were evaluated for publication bias and heterogeneity. Weighted mean differences (WMDs) and 95% confidence intervals (CIs) were calculated. We also performed subgroup and sensitivity analyses. Results: A total of 11 trials contributed to the analysis. BP was shown to be efficacious in increasing BMD at the LS and TH. WMD in BMD absolute change was 0.21g/cm2 (95% CI, 0.13-0.28) at the LS and 0.27g/cm2 (95% CI, 0.02-0.12) at the TH. WMD in BMD percentage change was 5.42 (95% CI, 4.37-6.48) at the LS and 3.03 (95% CI, 2.01-4.01) at the TH. Subgroup analysis revealed that age difference, interventional duration, types of interventions and BP types were associated with variable effects on BMD at the LS and TH. Conclusions: BPs may protect against bone loss in postmenopausal women with EBC receiving adjuvant AI treatment. © 2014 IMSS.


PubMed | Peoples Hospital Of New District Longhua Shenzhen
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2014

Agranulocytosis is a rare and serious adverse effect of antithyroid drugs (ATD), in particular methimazole (MMI), and usually develops within 3 months following the start of uninterrupted ATD treatment. Agranulocytosis may also develop for the first time following interruption and subsequent resumption of the same ATD treatment. In this case report, a 27-year-old female, who was treated for thyrotoxicosis with MMI, developed agranulocytosis following the discontinuation of MMI treatment for four months. To the best of our knowledge, this is the first study to report this. The aim of this report is to increase the awareness of physicians of the onset of agranulocytosis when MMI is discontinued, and to demonstrate that MMI should be used with caution.

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