Peoples Hospital of Luzhou
Peoples Hospital of Luzhou
Yang G.,Peoples Hospital of Luzhou
European review for medical and pharmacological sciences | Year: 2017
OBJECTIVE: Diabetes affects the renal function at a certain stage. Oral medication glipizide plays a hypoglycemic effect mainly through releasing insulin, while more insulin is derived from islet β cells. It is still controversy whether antidiabetics. This study mainly intends to investigate the role of glipizide in inhibiting renal interstitial fibrosis.MATERIALS AND METHODS: A total of 93 SD rats were purchased from Guangdong animal monitoring and established unilateral ureteral obstruction (UUO) model to simulate renal interstitial fibrosis. Forty rats in the experimental group received glipizide intraperitoneal injection for a week at 30 days after modeling, while another 40 rats in the control group received a normal saline injection. The last 10 rats were treated as blank group. Hematoxylin and eosin (HE) staining was applied to test renal interstitial fibrosis. Immunohistochemistry was used to detect fibronectin expression in glomerular and renal tubules. AKT signaling pathway related factors expression was measured by Western blot to determine AKT signal activation.RESULTS: HE staining showed that the entire kidney cytoplasm red dye becomes shallow, renal medulla gradually disappears, renal tubular epithelial cells enlarge, vacuoles degeneration, renal tubule and collecting tube expansion, inflammatory cells infiltration after UUO modeling. Glipizide treatment decreased dilated renal tubule number, improved glomerulus integrity, and reduced inflammatory infiltration. Fibronectin level in the experimental group was significantly lower than that in control (p<0.05). Western blot revealed that p-AKT expression downregulated after glipizide treatment.CONCLUSIONS: Glipizide blocks renal interstitial fibrosis by inhibiting AKT signaling pathway.
Yang J.,Peoples Hospital of Luzhou |
Li Z.-X.,Luzhou Medical College
Journal of Sichuan University (Medical Science Edition) | Year: 2015
Objective To explore the effects of the Hemopexin (Hx) on the generation of free radicals and brain edema after intracerebral hemorrhage (ICH). Methods One hundred SD rats were randomly and evenly divided into four groups (25 rats in each group), Which named Sham group, ICH control group, Hx removal group and Hx intervention group respectively. There were five observation points (1 d, 3 d, 7 d, 14 d, 21 d) and which contain five rats for each. The stereotactic injection technique was used to make the ICH model, which adopted rat autologous whole blood that was removed or mixed with Hx and then injected to the right caudate nucleus of the brain. Sham group were only injected with 50 μL saline to the right caudate nucleus and ICH control group were injected with 50 μL autologous whole blood; Hx removal group were injected 50 μL autologous whole blood of removal Hx and Hx intervention group were injected 50 μL autologous whole blood which contain 0. 25 mg (5 μg/μL) Hx. Bederson's method was applied to evaluate whether the model was established successfully or not. Garcia' s method was used to estimate the neurological dysfunction scores by. Water contents of brain tissue around the hematoma was detected by dry-wet weigh method. The superoxide dismutase (SOD) activity were measured with the xanthine oxidase method. The content of the malonyldialdehyde (MDA) was measured by the thiobarbituric acid method. Pathological changes of brain tissue around the hematoma were detected by immunohistochemical method at each observation time points; and the immunohistochemical scores result was judged by the double semiquantitative evaluation method. Results Compared with Sham group, at 3-21 d, there were statistically significant differences (P<0. 05) in the neurological disorders and water content of the brain tissue and immunohistochemistry scores within ICH control group, Hx intervention group and Hx removal group. Compared with Sham group, at 1-21 d, there were statistically significant differences (P<0. 05) in SOD activity and the content of the MDA within ICH control group, Hx intervention group and Hx removal group. All the indexes above were superior in Hx intervention group to ICH control group (P<0. 05), and inferior in Hx removal group to ICH control group (P<0. 05). Conclusion The Hemopexinmay attenuate the generation of the free radicals and encephalaedema in the brain tissue around the hematoma after intracerebral hemorrhage.
Yin H.-P.,Peoples Hospital of Luzhou |
Wang C.-M.,Peoples Hospital of Luzhou
International Eye Science | Year: 2016
AIM: To observe and study the comprehensive application effect of hydrochloric cyclopentolate eye drops in the mydriasis test and optometry for children with hyperopia. METHODS: Eighty-four children with hyperopia who were intervened with mydriasis test and optometry in our hospital from February 2014 to March 2015 were selected as the research object, and they were intervened with mydriasis test and optometry by tropicamide or hydrochloric cyclopentolate eye drops. The diopter, pupil diameter and residual regulation before administration and at different time after administration of the two methods were compared, and the detected results of the two groups with different severity degree were compared too. RESULTS: The diopter, pupil diameter and residual regulation before administration of the two eye drops had no significant differences(all P>0.05), while the residual regulation after using hydrochloric cyclopentolate eye drops at 20, 40, 60min and 24h were all smaller than those after using tropicamide(all P<0.05). The pupil diameter after using the two methods at 60min had no significant differences (P>0.05). The pupil diameter of the two groups at 48h after administration both had no significant differences to those before administration(all P>0.05). CONCLUSION: The comprehensive application effect of hydrochloric cyclopentolate eye drops in the mydriasis test and optometry of children with hyperopia is better, and its paralysis effect for ciliaris is obvious. Copyright 2016 by the IJO Press.
Zheng W.,Peoples Hospital of Luzhou
Clinical Nuclear Medicine | Year: 2016
ABSTRACT: A 15-year-old man with acute lower back pain for 7 days underwent F-NaF PET/CT to determine the cause of his symptoms. The PET images revealed irregularly increased F activity in the L1 vertebral body without definite sclerotic changes on CT. However, the corresponding CT images revealed an adjacent paravertebral mass extending into the vertebral foramen without elevated activity on PET. A diagnosis of Burkitt lymphoma was made after pathological examination. Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.
Xu J.,University of Sichuan |
Liu L.,University of Sichuan |
Zheng X.,Peoples Hospital of Luzhou |
You C.,University of Sichuan |
Li Q.,University of Sichuan
Neurological Research | Year: 2012
Background and purpose: Craniopharyngioma is a common intracranial tumor characterized by high recurrence rate and poor prognosis in spite of multidisciplinary approaches. The ADAM-like decysin 1 (ADAMDEC1) is a member of a disintegrin and metalloprotease (ADAM) family which correlates with tumor progression and aggressive behavior. This study aimed to detect and inhibit expression of ADAMDEC1 in order to see whether craniopharyngioma cell growth could be suppressed. Methods: ADAMDEC1 expression was detected by Western Blot analysis and reverse transcriptionpolymerase chain reaction (RT-PCR). Craniopharyngioma cells, which were obtained from tumor samples after surgical removal, were cultured with or without tamoxifen. MTT assay was used to examine tumor cell growth. Results: ADAMDEC1 mRNA was expressed in craniopharyngioma cell cultures, but it was not shown in normal brain tissue. Tamoxifen not only reduced ADAMDEC1 mRNA and protein expression, but also inhibited craniopharyngioma cell proliferation. Conclusions: ADAMDEC1 may serve as a novel marker for craniopharyngiomas, and tamoxifen could inhibit craniopharyngioma cell growth and ADAMDEC1 expression. © W. S. Maney & Son Ltd 2012.
Zheng W.-W.,Peoples Hospital of Luzhou |
Zhao L.,Luzhou Medical College |
Wei Y.-M.,Luzhou Medical College |
Ye Y.,Luzhou Medical College |
Xiao S.-H.,Luzhou Medical College
Chemical and Pharmaceutical Bulletin | Year: 2010
The objective of this study was to develop and evaluate nanoemulsion system for transdermal delivery of granisetron hydrochloride. Pseudo-ternary phase diagram was constructed to ascertain the concentration range of components of nanoemulsion composed of isopropyl myristate (IPM) as an oil phase, tween 85 as surfactant, ethanol as cosurfactant, water as aqueous phase. The effects of the content of IPM as an oil phase and n-methyl pyrrolidone (NMP) as transdermal enhancer on rat skin permeation of granisetron hydrochloride nanoemulsion were studied in vitro. The results showed that the mean particle size of nanoemulsion ranged from 50.4±1.5 to 82.4±0.9 nm with homogeneous size distribution. The resulted optimum formulation composed of 2.5% granisetron hydrochloride, 4% IPM, 40% tween 85/ethanol (1 : 1) and 10% NMP showed that the skin permeation rate was the highest (85.39±2.90 μg/cm 2/h) and enhancement of drug permeability was 4.1-folde for transdermal delivery of granisetron hydrochloridein comparison with the control group (20% of tween 85 and 20% of ethanol micelle solution containing 2.5% of granisetron hydrochloride without IPM), and cumulative permeation amount was the highest (891.8±2.86 μg/cm 2) with the shortest lag time (0.11±0.02 h) and was stable for at least 12 months. Therefore, the nanoemulsion system developed in this study offers a promising vehicle for the transdermal delivery system of granisetron hydrochloride, which may be as effective as oral or intravenous dosage forms and avoid some difficulties associated with these dosage forms. © 2010 Pharmaceutical Society of Japan.
Zhao L.,Luzhou Medical College |
Wei Y.-M.,Luzhou Medical College |
Yu Y.,Chongqing Medical University |
Zheng W.-W.,Peoples Hospital of Luzhou
Archives of Pharmacal Research | Year: 2010
blends of polyvinyl pyrrolidone (PVP) and ethyl cellulose (EC) could improve the vitro release behavior of the poorly water-soluble drug nifedipine. Hollow microspheres containing nifedipine were prepared by a solvent diffusion-evaporation method using various ratios of PVP and EC codissolved with drug in ethanol/ether (5:1, v/v). The hollow microspheres could float in release medium for more than 24 h, and floating capacities were not be influenced by mixing PVP. In vitro release profiles of hollow microspheres prepared using EC along showed an initial burst release to some extent, and the cumulative release percentage was less than 55% after 24 h. But, not only the slope but also the shape of the release curves was affected by using mixture of PVP and EC. What's more important, when the ratio (PVP/EC) increased to 1.5:8.5, the cumulative release percentage could be increased to 95.8%. Furthermore, the release rate of microspheres showed a zero order approximate dynamic model and could be expressed by the following equation: Q=3.78t+8.52 (r=0.990). Consequently, hollow microspheres prepared using polymer blends of PVP and EC (1.5:8.5, w/w) could be suitable for floating-type controlled-release delivery systems for the oral administration of nifedipine.
Zhou X.,Luzhou Medical College |
Liu Z.,University of Houston |
Jang F.,Luzhou Medical College |
Xiang C.,Peoples Hospital of Luzhou |
And 2 more authors.
PLoS ONE | Year: 2012
Hedgehog signaling plays critical roles in pancreatic oncogenesis and chronic pancreatitis, but its roles in acute pancreatitis (AP) are largely ambiguous. In this study, we provide evidence that Sonic hedgehog (Shh), but neither Desert hedgehog (Dhh) nor Indian hedgehog (Ihh), is the main protein whose expression is activated during the development of cerulein-induced acute pancreatitis in mice, and the Shh serves as an anti-inflammation factor in an autocrine manner. Blocking autocrine Shh signaling with anti-Shh neutralizing antibody aggravates the progression of acute pancreatitis. Mechanistic insight into Shh signaling activation in acute pancreatitis indicates that inflammatory stimulation activates Shh expression and secretion, and subsequently upregulates the expression and secretion of interleukin-10 (IL-10). Moreover, inhibition of Shh signaling with neutralizing antibody abolishes IL-10 production in vivo and in vitro. Molecular biological studies show that autocrine Shh signaling activates the key transcriptional factor Gli1 so that the target gene IL-10 is upregulated, leading to the protective and anti-inflammatory functions in the mouse model of acute pancreatitis. Thus, this study suggests autocrine Shh signaling functions as a protective signaling in the progression of acute pancreatitis. © 2012 Zhou et al.
PubMed | Peoples Hospital of Luzhou
Type: Journal Article | Journal: Clinical nuclear medicine | Year: 2016
A 59-year-old woman with back pain underwent an F-NaF PET/CT bone scan. Unexpectedly, multiple foci of increased tracer uptake were present in the abdomen and pelvis, which corresponded to the calcified soft tissue masses. The subsequent F-FDG PET/CT not only confirmed increased FDG activity in these partially calcified mass but also revealed abnormal activity in noncalcified lesions. The pathological examination demonstrated that the patient had ovarian cancer.
PubMed | Peoples Hospital of Luzhou
Type: Case Reports | Journal: Clinical nuclear medicine | Year: 2016
A 15-year-old girl experienced an alveolar soft part sarcoma in the right thigh 3 years ago, which was resected. Postsurgical recovery was uneventful until approximately 1 year ago when she began to feel mild local tenderness and gradual swelling around the surgical scars. The patient underwent Tc-MDP bone scintigraphy to evaluate osseous metastases. Although no lesion in the bone was identified, the images showed abnormally increased activity in the region of known focal lesion.