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Yang J.,Peoples Hospital of Luzhou | Li Z.-X.,Luzhou Medical College
Journal of Sichuan University (Medical Science Edition) | Year: 2015

Objective To explore the effects of the Hemopexin (Hx) on the generation of free radicals and brain edema after intracerebral hemorrhage (ICH). Methods One hundred SD rats were randomly and evenly divided into four groups (25 rats in each group), Which named Sham group, ICH control group, Hx removal group and Hx intervention group respectively. There were five observation points (1 d, 3 d, 7 d, 14 d, 21 d) and which contain five rats for each. The stereotactic injection technique was used to make the ICH model, which adopted rat autologous whole blood that was removed or mixed with Hx and then injected to the right caudate nucleus of the brain. Sham group were only injected with 50 μL saline to the right caudate nucleus and ICH control group were injected with 50 μL autologous whole blood; Hx removal group were injected 50 μL autologous whole blood of removal Hx and Hx intervention group were injected 50 μL autologous whole blood which contain 0. 25 mg (5 μg/μL) Hx. Bederson's method was applied to evaluate whether the model was established successfully or not. Garcia' s method was used to estimate the neurological dysfunction scores by. Water contents of brain tissue around the hematoma was detected by dry-wet weigh method. The superoxide dismutase (SOD) activity were measured with the xanthine oxidase method. The content of the malonyldialdehyde (MDA) was measured by the thiobarbituric acid method. Pathological changes of brain tissue around the hematoma were detected by immunohistochemical method at each observation time points; and the immunohistochemical scores result was judged by the double semiquantitative evaluation method. Results Compared with Sham group, at 3-21 d, there were statistically significant differences (P<0. 05) in the neurological disorders and water content of the brain tissue and immunohistochemistry scores within ICH control group, Hx intervention group and Hx removal group. Compared with Sham group, at 1-21 d, there were statistically significant differences (P<0. 05) in SOD activity and the content of the MDA within ICH control group, Hx intervention group and Hx removal group. All the indexes above were superior in Hx intervention group to ICH control group (P<0. 05), and inferior in Hx removal group to ICH control group (P<0. 05). Conclusion The Hemopexinmay attenuate the generation of the free radicals and encephalaedema in the brain tissue around the hematoma after intracerebral hemorrhage.

Bai Y.,Wenzhou University | Lu H.,Wenzhou University | Zhang G.,Peoples Hospital of Luzhou | Wu C.,Wenzhou University | And 3 more authors.
Life Sciences | Year: 2014

Aims Sedum sarmentosum Bunge, a traditional Chinese herbal medicine, has a wide range of clinical effects, including anti-oxidation, anti-inflammation, and anti-cancer properties. In this study, we determined whether S. sarmentosum Bunge Extract (SSBE) has anti-fibrotic effects on renal tissues. Main methods We investigated the effects of SSBE on aristolochic acid (AA)-induced injury to renal tubular epithelial cells (RTECs) in vitro and unilateral ureteral obstruction (UUO)-induced renal fibrosis in vivo by evaluating epithelial-to-mesenchymal transition (EMT) and the accumulation of extracellular matrix (ECM) components. Furthermore, we examined the expression levels of TGF-β1 and its receptor. Key findings In cultured RTECs (NRK-52E), AA promoted renal EMT and ECM accumulation by up-regulating the expression of mesenchymal markers and ECM components and by down-regulating the expression of epithelial markers. In addition, AA induced an imbalance between MMP-2 and TIMP-2 and enhanced expression of TGF-β1 and its receptor. SSBE treatment significantly inhibited AA-induced TGF-β1 expression and prevented the induction of EMT and deposition of ECM. In the UUO rats, tubular injury and interstitial fibrosis were obviously increased. SSBE administration protected renal function, as indicated by reduced serum creatinine levels, and alleviated renal interstitial fibrosis. These anti-fibrotic effects were associated with a reduction in TGF-β1 expression and inhibition of EMT and ECM accumulation. Significance These findings suggest that SSBE may have therapeutic potential for fibrotic kidney diseases. © 2014 Elsevier Inc.

Zheng W.,Peoples Hospital of Luzhou
Clinical Nuclear Medicine | Year: 2016

ABSTRACT: A 15-year-old man with acute lower back pain for 7 days underwent F-NaF PET/CT to determine the cause of his symptoms. The PET images revealed irregularly increased F activity in the L1 vertebral body without definite sclerotic changes on CT. However, the corresponding CT images revealed an adjacent paravertebral mass extending into the vertebral foramen without elevated activity on PET. A diagnosis of Burkitt lymphoma was made after pathological examination. Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.

Tang W.,University of Sichuan | Chen Z.,Peoples Hospital of Luzhou | Wu W.,Luzhou Medical College | Qiu H.,University of Sichuan | And 3 more authors.
Artificial Organs | Year: 2013

Rhabdomyolysis (RM) and subsequent myoglobin (Mb) deposition can lead to acute kidney injury. Continuous venovenous hemofiltration (CVVH) can remove Mb, but direct renal protection is unclear. We hypothesized that CVVH can improve renal mitochondrial dysfunction in its early stage. Twenty-four mongrel dogs were randomly divided into four groups: (A) control; (B) model; (C) model+CVVH (50mL/kg/h); and (D) model+CVVH (30mL/kg/h). RM was induced by glycerol via intramuscular injection. The dogs were closely monitored for urine flow and renal function. Mb, plasma tumor necrosis factor-α (TNF-α), and interleukin (IL)-6 were measured by enzyme-linked immunosorbent assay. After 8h of CVVH, the morphological changes of renal mitochondria were observed and mitochondrial function indicators (reactive oxygen species, malondialdehyde, and respiratory control index) were detected. Western blot analysis was used to detect the expression of Mb, TNF-α, and IL-6 in renal tubules. The terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay method and Western blot analysis were used to detect apoptosis and apoptosis-related proteins. In group B, the dog urine output gradually decreased with increased blood creatinine. In groups C and D, the urine output was normal and stable. CVVH effectively eliminated Mb. High-dose CVVH was significantly better for removal efficiency than low-dose CVVH. CVVH significantly reduced the deposition of circulating Mb in the kidney in a dose-dependent manner. The impact of CVVH on TNF-α and IL-6 were not observed. The morphological changes of mitochondria and function indicators were significantly improved in group C compared with groups D and B. Compared with group B, renal apoptosis and apoptosis-related protein expression were inhibited in groups C and D. Group C was significantly better for mitochondrial improvement and apoptosis inhibition than group D. At the cellular and molecular level, CVVH can improve renal mitochondrial function and inhibit cell apoptosis. Early CVVH can protect from RM-caused renal injuries in a dose-dependent manner. Artificial Organs © 2013 The International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc 37 4 April 2013 10.1111/j.1525-1594.2012.01574.x Main Text Articles Main Text Article © 2013, Copyright the Authors. Artificial Organs © 2013, International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc..

Zheng W.-W.,Peoples Hospital of Luzhou | Zhao L.,Luzhou Medical College | Wei Y.-M.,Luzhou Medical College | Ye Y.,Luzhou Medical College | Xiao S.-H.,Luzhou Medical College
Chemical and Pharmaceutical Bulletin | Year: 2010

The objective of this study was to develop and evaluate nanoemulsion system for transdermal delivery of granisetron hydrochloride. Pseudo-ternary phase diagram was constructed to ascertain the concentration range of components of nanoemulsion composed of isopropyl myristate (IPM) as an oil phase, tween 85 as surfactant, ethanol as cosurfactant, water as aqueous phase. The effects of the content of IPM as an oil phase and n-methyl pyrrolidone (NMP) as transdermal enhancer on rat skin permeation of granisetron hydrochloride nanoemulsion were studied in vitro. The results showed that the mean particle size of nanoemulsion ranged from 50.4±1.5 to 82.4±0.9 nm with homogeneous size distribution. The resulted optimum formulation composed of 2.5% granisetron hydrochloride, 4% IPM, 40% tween 85/ethanol (1 : 1) and 10% NMP showed that the skin permeation rate was the highest (85.39±2.90 μg/cm 2/h) and enhancement of drug permeability was 4.1-folde for transdermal delivery of granisetron hydrochloridein comparison with the control group (20% of tween 85 and 20% of ethanol micelle solution containing 2.5% of granisetron hydrochloride without IPM), and cumulative permeation amount was the highest (891.8±2.86 μg/cm 2) with the shortest lag time (0.11±0.02 h) and was stable for at least 12 months. Therefore, the nanoemulsion system developed in this study offers a promising vehicle for the transdermal delivery system of granisetron hydrochloride, which may be as effective as oral or intravenous dosage forms and avoid some difficulties associated with these dosage forms. © 2010 Pharmaceutical Society of Japan.

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