Shi Y.,Huazhong University of Science and Technology |
Shi Y.,Shanghai Genome Pilot Institutes for Genomics and Human Health |
Shi Y.,Changning Mental Health Center |
Li L.,Guangxi Province Tumor Hospital |
And 86 more authors.
Nature Genetics | Year: 2013
To identify new genetic risk factors for cervical cancer, we conducted a genome-wide association study in the Han Chinese population. The initial discovery set included 1,364 individuals with cervical cancer (cases) and 3,028 female controls, and we selected a 'stringently matched samples' subset (829 cases and 990 controls) from the discovery set on the basis of principal component analysis; the follow-up stages included two independent sample sets (1,824 cases and 3,808 controls for follow-up 1 and 2,343 cases and 3,388 controls for follow-up 2). We identified strong evidence of associations between cervical cancer and two new loci: 4q12 (rs13117307, Pcombined, stringently matched = 9.69 × 10-9, per-allele odds ratio (OR)stringently matched = 1.26) and 17q12 (rs8067378, Pcombined, stringently matched = 2.00 × 10-8, per-allele ORstringently matched = 1.18). We additionally replicated an association between HLA-DPB1 and HLA-DPB2 (HLA-DPB1/2) at 6p21.32 and cervical cancer (rs4282438, Pcombined, stringently matched = 4.52 × 10-27, per-allele ORstringently matched = 0.75). Our findings provide new insights into the genetic etiology of cervical cancer. © 2013 Nature America, Inc. All rights reserved.
Li X.,Capital Medical University |
Zhang C.,Chinese Academy of Sciences |
Shi Q.,Handan Maternal and Child Health Hospital |
Yang T.,Peoples Hospital of Liuzhou |
And 13 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2013
Background. Herb-derived compound andrographolide sulfonate (called Xiyanping injection) recommended control measure for severe hand, foot, and mouth disease (HFMD) by the Ministry of Health (China) during the 2010 epidemic. However, there is a lack of good quality evidence directly comparing the efficacy of Andrographolide Sulfonate combination therapy with conventional therapy. Methods. 230 patients were randomly assigned to 7-10 days of Andrographolide Sulfonate 5-10 mg/Kg/day and conventional therapy, or conventional therapy alone. Results. The major complications occurred less often after Andrographolide Sulfonate (2.6% versus 12.1%; risk difference [RD], 0.94; 95% CI, 0.28-1.61; P = 0.006). Median fever clearance times were 96 hours (CI, 80 to 126) for conventional therapy recipients and 48 hours (CI, 36 to 54) for Andrographolide Sulfonate combination-treated patients (χ 2 = 16.57, P < 0.001). The two groups did not differ in terms of HFMD-cause mortality (P = 1.00) and duration of hospitalization (P = 0.70). There was one death in conventional therapy group. No important adverse event was found in Andrographolide Sulfonate combination therapy group. Conclusions. The addition of Andrographolide Sulfonate to conventional therapy reduced the occurrence of major complications, fever clearance time, and the healing time of typical skin or oral mucosa lesions in children with severe HFMD. © 2013 Xiuhui Li et al.
Yang G.,Guangxi Medical University |
Liu R.,Guangxi Medical University |
Peng P.,Guangxi Medical University |
Long L.,Guangxi Medical University |
And 9 more authors.
PLoS ONE | Year: 2014
Background: Myocardial siderosis is the most common cause of death in patients with beta thalassemia major(TM). This study aimed at investigating the occurrence, prevalence and severity of cardiac iron overload in a young Chinese population with beta TM. Methods and Results: We analyzed T2*cardiac magnetic resonance (CMR), left ventricular ejection fraction (LVEF) and serum ferritin (SF) in 201 beta TM patients. The median age was 9 years old. Patients received an average of 13 units of blood per year. The median SF level was 4536 ng/ml and 165 patients (82.1%) had SF>2500 ng/ml. Myocardial iron overload was detected in 68 patients (33.8%) and severe myocardial iron overload was detected in 26 patients (12.6%). Twenty-two patients ≤10 years old had myocardial iron overload, three of whom were only 6 years old. No myocardial iron overload was detected under the age of 6 years. Median LVEF was 64% (measured by CMR in 175 patients). Five of 6 patients with a LVEF<56% and 8 of 10 patients with cardiac disease had myocardial iron overload. Conclusions: The TM patients under follow-up at this regional centre in China patients are younger than other reported cohorts, more poorly-chelated, and have a high burden of iron overload. Myocardial siderosis occurred in patients younger than previously reported, and was strongly associated with impaired LVEF and cardiac disease. For such poorly-chelated TM patients, our data shows that the first assessment of cardiac T2*should be performed as early as 6 years old. © 2014 Yang et al.
Chen Y.-J.,Guangxi Medical University |
Chen J.,Peoples Hospital of Liuzhou |
Yang L.,Peoples Hospital of Liuzhou |
Jiang Y.-M.,Peoples Hospital of Liuzhou |
And 2 more authors.
Chinese Journal of Cancer Prevention and Treatment | Year: 2016
OBJECTIVE: Cervical cancer is the second most common cancer globally and cervical intraepithelial neoplasia was known as precancerous lesions. The aims of this study were to investigate the influencing factors of cervical intraepithelial neoplasia(CIN), the relationships between high risk human-papillomavirus(HR-PCR) or Chlamydia trachomatis(CT) and CIN, METHODS: We performed a hospital-base cases control study for 4 years. A total of 12 644 outpatients were involved in this study for the cervical cancer screening program. Cervical swabs were obtained to detect CT, HR-PCR and TCT. Cervical biopsies were taken when it was necessary and recorded the infectious status of HR-HPV and CT. RESULTS: Totally 260 CIN patients were identified(2.06%), The relative risk for CIN increased when HR-HPV(OR=18.516), CT positive(OR=2.602), growth of numbers of pregnancies(OR=1.477) and age(OR=1.690) in the multiple Logistic regression model. We stratified further according to the different status of HR-HPV and CT infection. The prevalence of CIN was the highest in group of HR-HPV, CT both positive(18.00%), than in groups of HR-HPV positive(10.26%), CT positive(1.97%) and group of HR-HPV, CT both negative(0.49%). The difference was statistically significant(χ2=875.927, P<0.001). CONCLUSIONS: The study indicates that increasing of age and frequency of pregnancy are influencing factors of CIN, HR-HPV plays the important role and a possible contribution of CT in cervical carcinogenesis. It is also implied co-infection of HR-HPV and CT increased risk for development of CIN. © 2016, Editorial Board of Chinese Journal of Cancer Prevention and Treatment. All right reserved.
Yuan L.,Peoples Hospital of Liuzhou |
Xu Y.-X.,Peoples Hospital of Liuzhou |
Chen J.,Peoples Hospital of Liuzhou |
Qin Y.-Y.,Peoples Hospital of Liuzhou |
Chen G.-L.,Peoples Hospital of Liuzhou
Chinese Journal of Cancer Prevention and Treatment | Year: 2013
OBJECTIVE: To study the relationship between negative life events expoure and cervical carcinoma among high risk human papillomavirus (HR-HPV).METHODS: A 15-year retrospective study was carried out in a cohort of 60 cervical carcinoma with HR-HPV infection, at the same time 1:1 matched (age, gender, ethnic, education)60 participants of HR-HPV without cervical lesion were selected. Logistic regression analysis was used to explore the associations between behaviors, diet habits psychological factors and HR-HPV.RESULTS: Serum Zn and Se levels were significantly lower in patients with cervical cancer [(0.719±0.623) and (0.079±0.035) mg/mL] than in control subjects [(1.109±0.121) and (0.208±0.032) mg/mL].In the study cases, the mean of selenium and zinc were significantly lower than those of controls (t=6.094, P=0.004; t=8.531, P=0.002). A close negative correlation was found between the amount of protein and fruit thinning intake with the risk of cervical carcinoma among HR-HPV, and there was dose response relationship. There were no relationships between HR-HPV and using contraceptives, age at first sexual intercourse, multiple sexual partners, but it related to one to ten years psychic trauma. CONCLUSION: There was a synergic intraction between HR-HPV infection and low selenium, low zinc, low protein and fruit thinning intakes in a long term, one to ten years psychic trauma.
Li J.-F.,Peoples Hospital of Liuzhou |
Wen L.-Q.,Peoples Hospital of Liuzhou |
Liu F.-Y.,Central South University |
Liu H.,Central South University |
Peng Y.-M.,Central South University
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010
BACKGROUND: It has confirmed that angiotensin converting enzyme inhibitor benazepril can delay fibrosis of varied organs. However, whether benazepril has inhabited effect on peritoneal fibrosis in the process of peritoneal dialysis is poorly understood. OBJECTIVE: It is assumed that benazepril could inhabit peritoneal fibrosis of peritoneum with peritoneal dialysis, in addition, to compare the effect to other mehods. METHODS: All rats were randomly and evenly divided into 4 groups. There was no intervention in the control group; saline solution, and 20 mL 42.5 g/L Dianeal solution, was injected into rats in the saline solution and peritoneal dialysis groups; in the combination group, 20 mL 42.5 g/L Dianeal solution was injected combined with oral taken benazepril 20 mg/(kgd). The intraperitoneal injection performed once a day, for 4 successive weeks. The ultrafiltration function was performed 4 weeks later. Meantime, Paraffin sections were cut and stained by Van Gieson to measure peritoneal thickness. RESULTS AND CONCLUSION: Two rats in the peritoneal dialysis group and 1 rat in the combination group were dead. The remained 37 rats were included in the final analysis. Compared to the control and saline solution groups, the ultrafiltration volume of the peritoneal dialysis and combination groups were obviously decreased (P all < 0.05), especially notably decreased in the combination group (P < 0.05). Compared to the control group and saline solution groups, the peritoneal thickness was significantly elevated in the combination group, but not as much as in the peritoneal dialysis group (P < 0.05). In the long-term peritoneal dialysis rats, administration of benazepril can effectively protect the ultrofiltration function of peritoneum and delay the progression of peritoneal fibrosis.
Zhou Y.,Peoples Hospital of Liuzhou |
Hu Z.,Peoples Hospital of Liuzhou |
Li N.,Peoples Hospital of Liuzhou |
Jiang R.,Peoples Hospital of Liuzhou
International Journal of Molecular Medicine | Year: 2015
As a pro-inflammatory cytokine, interleukin-32 (IL-32) is reported to play an important role in tumor development and progression. However, its effects on the invasion and motility of osteosarcoma cells remain elusive. The aim of the present study was to determine the molecular mechanisms of IL-32 in osteosarcoma cells using RT-PCR and western blot analysis. The results showed that IL-32 stimulation dose-dependently promoted the invasion and motility of osteosarcoma cells. Knockdown of endogenous IL-32 by siRNA inhibited osteosarcoma cell invasion and motility. Moreover, IL-32 induced the activation of AKT in a time-dependent manner. IL-32 stimulationwas also capable of increasing the expression and secretion of matrix metalloproteinase (MMP)-13, which is involved in tumor invasion and metastasis. In addition, blockade of AKT activation suppressed IL-32-mediated invasion, motility and MMP-13 upregulation in osteosarcoma cells. Taken together, our results suggest that IL-32 stimulation promotes the invasion and motility of osteosarcoma cells, possibly via the activation of AKT and the upregulation of MMP-13 expression. Thus, IL-32 may serve as a marker for diagnosis, as well as for the treatment of osteosarcoma.