Peoples Hospital of Linzi District

Zibo, China

Peoples Hospital of Linzi District

Zibo, China
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Zhuang X.,Peoples Hospital of Linzi District | Dong A.,Peoples Hospital of Linzi District | Wang R.,Peoples Hospital of Linzi District | Cao H.,Peoples Hospital of Linzi District
Saudi Journal of Biological Sciences | Year: 2017

The present study investigates the effect of matrine on colon cancer cell viability and apoptosis and tumor growth in mice xenograft model. The results from MTT assay revealed a concentration and time dependent reduction in viability of HCT8 and HT29 colon cancer cells by matrine. The viability of HCT8 and HT29 cells was reduced to 24.67 and 29.32% on treatment with 4. μM/ml concentration of matrine after 48. h (P. <. 0.05). The results from flow cytometry revealed increase in population of HCT8 and HT29 cells to 77.6. ±. 0.3 and 54.0. ±. 5.4%, respectively compared to 1.4. ±. 0.3 and 2.4. ±. 0.7% in control on exposure to 1. μM/ml concentration of matrine. Histone H2AX phosphorylation and expression of Myt1, cyclin A2, cyclin B1 and p53 were increased in HCT8 and HT29 cells on treatment with matrine for 48. h. Matrine treatment also increased the phosphorylation of cdc2 significantly compared to control cells at 48. h (P. <. 0.05). Results from Annexin-V/FITC-staining showed increase in proportion of apoptotic cells in HCT8 and HT29 cells 67.52 and 68.56 on treatment with 1. μM/ml of matrine. Matrine treatment caused a marked reduction in the growth of HCT8 cell xenograft after 21. days. Thus matrine inhibits cell viability, induces apoptosis and inhibits tumor growth in colon cancer. © 2017 King Saud University.


Zhuang X.,Peoples Hospital of Linzi District | Dong A.,Peoples Hospital of Linzi District | Wang R.,Peoples Hospital of LinziDistrict | Shi A.,Peoples Hospital of Linzi District
Saudi Journal of Biological Sciences | Year: 2017

Background: The current study was designed to investigate the effect of crocetin on the proliferation inhibition of colon cancer cells and the underlying mechanism. Methods: MTT assay showed inhibition of proliferation of colon cancer cells in a dose based manner by crocetin treatment. At 30. μM concentration of crocetin proliferation rate of colon cancer cells was reduced to 14% after 24. h. Flow cytometry and fluorescence microscopy revealed induction of apoptosis in colon cancer cells on treatment with crocetin. The tube formation was suppressed significantly in the cultures of HUVEC treated with 30. μM concentration of crocetin compared to the control cultures. Results: The results from transwell assay revealed a significant reduction in the population of DU-145 cells passing through filters of transwell on treatment with crocetin compared to the control cells. Treatment of the DU-145 cells with crocetin caused a significant reduction in the expression levels of NF-κB, VEGF and MMP-9. The results from RT-PCR analysis revealed a significant reduction in the expression of genes involved in inflammation including, HMGB1, IL-6 and IL-8 on treatment of DU-145 cells with crocetin. However, the expression of NAG-1 gene was increased by crocetin treatment in DU-145 cells significantly compared to the control cells. Conclusion: Crocetin inhibits growth of colon cancer cells and prevents tube formation through induction of apoptosis. Therefore, crocetin can be used efficiently for the treatment of colon cancer. © 2017 King Saud University.


Jia M.-G.,Peoples Hospital Of Linzi District | Gao L.,Peoples Hospital Of Linzi District
Latin American Journal of Pharmacy | Year: 2017

A new heterocycles compound (S)-2-(1-amino-propyl)-5-fluoro-3-phenyl-3H-quinazolin-4-one trifluoroacetate (1), designed using 2-fluoro-6-nitro-benzonic acid (2) as start material, was successfully obtained via multiple synthesis route and finally characterized by IR,1H NMR, and single crystal X-ray crystallography. In addition, in vitro anticancer activity of the title compound (1) on three human liver cancer cells (HCCLM3, SMMC-7721 and HB611) was further determined. © 2017, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.


Yang X.,Peoples Hospital Of Linzi District | Wang X.,Peoples Hospital Of Linzi District | Shen H.,Linyi Peoples Hospital | Deng R.,Lunan Pharmaceutical Group Co. | Xue K.,Linyi Peoples Hospital
Cell Biochemistry and Biophysics | Year: 2015

Circulating miR-21 is upregulated in breast cancer. However, correlation of miR-21 expression with clinic pathologic characteristics remains questionable. In this study, we investigate whether combination of circulation miR-21 with circulating tumor cells (CTCs) marker (EpCAM, MUS1, HER2) could improve diagnostic specificity of metastatic breast cancer. Total 223 breast cancer patients were included. 89 % patients were associated with upregulation of miR-21 compared with health control. 20 % patients were detected for CTCs marker positive. For higher specificity purpose, triple marker positive samples were selected as true CTCs positive, which only occupied 59.5 % of total metastatic breast cancer patients. Specificity of detection of CTCs was 96.7 %. Furthermore, 59.5 % metastatic breast cancer patients were shown both abnormal miR-21 and true CTCs positive according to distribution of true CTCs positive and abnormal miR-21; Combination of miR-21 and CTCs was increased specificity of metastatic detection to 100 %. Our findings suggested that combination of miR-21 with CTCs marker could be used for better diagnosis of metastatic breast cancer in the future. © 2015, Springer Science+Business Media New York.


PubMed | Lunan Pharmaceutical Group Co., Peoples Hospital of Linzi District and Linyi Peoples Hospital
Type: Journal Article | Journal: Cell biochemistry and biophysics | Year: 2016

Circulating miR-21 is upregulated in breast cancer. However, correlation of miR-21 expression with clinic pathologic characteristics remains questionable. In this study, we investigate whether combination of circulation miR-21 with circulating tumor cells (CTCs) marker (EpCAM, MUS1, HER2) could improve diagnostic specificity of metastatic breast cancer. Total 223 breast cancer patients were included. 89% patients were associated with upregulation of miR-21 compared with health control. 20% patients were detected for CTCs marker positive. For higher specificity purpose, triple marker positive samples were selected as true CTCs positive, which only occupied 59.5% of total metastatic breast cancer patients. Specificity of detection of CTCs was 96.7%. Furthermore, 59.5% metastatic breast cancer patients were shown both abnormal miR-21 and true CTCs positive according to distribution of true CTCs positive and abnormal miR-21; Combination of miR-21 and CTCs was increased specificity of metastatic detection to 100%. Our findings suggested that combination of miR-21 with CTCs marker could be used for better diagnosis of metastatic breast cancer in the future.


Wang T.,Shandong Jiaotong Hospital | Dong A.-H.,Peoples Hospital of Linzi District | Cao H.-Y.,Peoples Hospital of Linzi District
Genetic Testing and Molecular Biomarkers | Year: 2016

Context: Slow coronary flow (SCF) is a special coronary microvascular disorder associated with recurrent chest pain. The pathogenesis of SCF remain unclear. Objectives: We sought to assess whether serum salusin-β levels are correlated with SCF. Methods: We enrolled 76 patients with angiographically confirmed SCF and 108 age- and gender-matched controls. We measured serum salusin-β levels by enzyme-linked immunosorbent assay and coronary flow rate was assessed using thrombolysis in myocardial infarction frame count (TFC). Results: Serum salusin-β levels were elevated in SCF patients compared with controls (4.33 [range 3.52-5.87] nmol/L vs. 3.76 [range 2.98-4.67] nmol/L). Multivariate logistic regression analysis revealed that salusin-β in serum was the independent predictor of SCF (odds ratio = 1.814). Serum salusin-β levels were independently correlated with mean-TFC (r = 0.355, p = 0.002). Conclusions: Serum salusin-β levels were independently associated with SCF. Therefore, our findings implicate a potential role of salusin-β in the pathophysiology of SCF and provide insights on both risk stratification and modification in this patient population. © Copyright 2016, Mary Ann Liebert, Inc.


PubMed | Peoples Hospital of Linzi District and Shandong Jiaotong Hospital
Type: Journal Article | Journal: Genetic testing and molecular biomarkers | Year: 2016

Slow coronary flow (SCF) is a special coronary microvascular disorder associated with recurrent chest pain. The pathogenesis of SCF remain unclear.We sought to assess whether serum salusin- levels are correlated with SCF.We enrolled 76 patients with angiographically confirmed SCF and 108 age- and gender-matched controls. We measured serum salusin- levels by enzyme-linked immunosorbent assay and coronary flow rate was assessed using thrombolysis in myocardial infarction frame count (TFC).Serum salusin- levels were elevated in SCF patients compared with controls (4.33 [range 3.52-5.87] nmol/L vs. 3.76 [range 2.98-4.67] nmol/L). Multivariate logistic regression analysis revealed that salusin- in serum was the independent predictor of SCF (odds ratio=1.814). Serum salusin- levels were independently correlated with mean-TFC (r=0.355, p=0.002).Serum salusin- levels were independently associated with SCF. Therefore, our findings implicate a potential role of salusin- in the pathophysiology of SCF and provide insights on both risk stratification and modification in this patient population.


Wang H.,Peoples Hospital of Linzi District | Yang X.,Peoples Hospital of Linzi District
International Journal of Clinical and Experimental Pathology | Year: 2015

Recently, there is growing evidence that tight junction proteins are often abnormally regulated in human tumors. The function of tight junction proteins in the maintenance of normal epithelial physiology has been well discussed, but their role in the tumorigenesis of gastric cancer is less well defined. To explore the expression distinction of the tight junction proteins claudin-1, -3, and -4 expression in the gastric cancer, the expression of claudin-1, -3, and -4 in 92 gastric cancer tissues and the non-neoplastic tissues adjacent to the tumors were examined by immunohistochemistry. Compared with adjacent non-neoplastic tissues, the expression of claudin-1 was down regulated. However, the expression of claudin-3 and claudin-4 were up-regulated in gastric cancer tissue. In addition, the expression of claudin-3 is correlated with claudin-4 expression in gastric cancer. Our present study reveals that claudin- 1, -3, and -4 protein expression altered between human gastric cancers and adjacent non-neoplastic tissues.


Qu H.,Weifang Peoples Hospital | Yang X.,Peoples Hospital Of Linzi District
Cell Biochemistry and Biophysics | Year: 2014

Accumulated evidences indicate metformin is associated with reduced risk of hepatocellular carcinoma (HCC) in diabetic patients, which inspired researchers to explore its therapeutic potentials in HCC. Since Hepatic stellate cells (HSCs) are believed to be the key contributors to tumor microenvironment in HCC and promotes tumor development, here, we explored the effect of metformin on tumor angiogenesis induced by interplay of HCC and HSCs. Our results showed that conditional medium from co-culture of HCC/HSCs induced VEGF secretions and stimulated human umbilical vein endothelial cells (HUVEC) tube formation. However, 25 µM metformin could inhibit this angiogenesis effect. Furthermore, knockdown AMPK of HSCs, not HCC, could abolish inhibition caused by metformin. Our finding suggested that metformin could inhibit HCC angiogenesis through targeting on HSCs through AMPK pathway. © 2014, Springer Science+Business Media New York.


PubMed | Peoples Hospital of Linzi District
Type: Journal Article | Journal: Chemical & pharmaceutical bulletin | Year: 2016

Chemical investigation of the sponge Dysidea sp. afforded three new sesquiterpene phenols (1-3) and one new sesquiterpene aminoquinone (4), together with four known sesquiterpene derivatives (5-8). The structures of all compounds were unambiguously elucidated by extensive spectroscopic analysis, as well as by comparison with the literature. The absolute configurations of compounds 1-4 were determined by electron capture detector (ECD) calculations and circular dichroism (CD) spectrum analysis. Their antibacterial activity against Escherichia coli (25922), Bacillus subtilis (6633), and Staphylococcus aureus (25923) were evaluated. Compounds 1 and 3 showed weak antibacterial activity against the above three strains, whereas compounds 4-8 showed potent antibacterial activities with minimum inhibitory concentration (MIC) values in the range of 3.125 to 12.5g/mL.

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