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Lin J.,Shandong University | Sun H.,Peoples Hospital of Liaocheng | Li J.,Shandong University | Zheng Y.,Shandong University | And 3 more authors.
Clinical Laboratory | Year: 2016

Background: Presepsin is a new emerging inflammatory biomarker. The primary purpose of this study was to elucidate the predictive usability of presepsin for severity assessment in patients with acute cholangitis (AC). Methods: A total of 119 treatment-naive patients with AC (64 males, 55 females) were enrolled in this study. Patients were classified with Grade I (mild), Grade II (moderate), or Grade III (severe) AC based on severity assessment guidelines. Presepsin concentrations were measured on admission. Results: The median presepsin concentrations were 297 pg/mL (interquartile range (IQR) 234 - 386 pg/mL), 590 pg/mL (IQR 559 - 619 pg/mL), and 857 pg/mL (IQR 740 - 960 pg/mL) in patients with mild, moderate, and severe AC, respectively. Presepsin concentrations were significantly higher in patients with severe AC than in patients with moderate AC (p < 0.01), and in patients with moderate AC than in patients with mild AC (p < 0.01). With the receiver operating characteristic (ROC) analysis, the areas under the curves (AUCs) for presepsin to discriminate patients with moderate and severe AC were 0.935 (95% confidence interval (CI) 0.877 to 0.993, p < 0.001) and 0.942 (95% CI 0.885 to 0.998, p < 0.001), respectively. Conclusions: Compared with other conventional biochemical indicators such as WBC, CRP, and PCT, presepsin may be a useful parameter for the severity assessment of AC. Source

Kang X.-J.,Peoples Hospital of the Xinjiang Uygur Autonomous Region | Shi X.-H.,Peoples Hospital of Liaocheng | Chen W.-J.,Peoples Hospital of the Xinjiang Uygur Autonomous Region | Pu X.-M.,Peoples Hospital of the Xinjiang Uygur Autonomous Region | And 8 more authors.
Clinical and Experimental Dermatology | Year: 2016

Background The KIT gene plays an important role in the pathogenesis of malignant melanoma (MM). In recent years, activating mutations in KIT have been recognized as oncogenic. A number of therapies have been established, which provide significant clinical benefits for patients with MM with KIT mutations. Thus, detection of KIT mutations can have profound therapeutic implications. Aim To investigate KIT gene expression in MMs in Chinese Uyghur and Han patients with mutations in KIT, and to identify the clinical features associated with KIT mutations and c-KIT expression. Methods In total, 105 MMs (56 from Uyghur and 49 from Han patients) were selected from patients in the Uyghur Autonomous region. Formalin-fixed, paraffin wax-embedded tumour sections were analysed for c-KIT expression using immunohistochemistry. Exons 11 and 13 of KIT were analysed for the presence of mutations using PCR amplification and DNA sequencing. Results Of the 105 MMs, 13 (10 Han and 3 Uyghur) were found to have mutations in KIT. Thus, the frequency of KIT mutations in Han patients was significantly higher than that in Uyghur patients (P = 0.02). We detected c-KIT expression in 71.4% and 42.9% of the tumour tissue samples collected from the Uyghur and Han patients, respectively. Conclusion In the Xinjiang Uyghur Autonomous Region in China, chronic sun-induced damage MM is the most prevalent MM among Chinese Uyghur patients, whereas acral and mucosal MMs are the most prevalent in Uyghur patients. Mutations in the KIT gene do not correlate with c-KIT expression. © 2015 British Association of Dermatologists. Source

Zhang L.,Hebei Medical University | Yuan L.,Peoples Hospital of Liaocheng | Li X.,Hebei Medical University | Wang Y.,Hebei Medical University | And 3 more authors.
Journal of Biomaterials and Tissue Engineering | Year: 2015

Objective: To investigate the effect of simvastatin on the release of MMP-9, MMP-12 and TIMP-1 in a rat COPD model, and its potential role in the treatment of COPD. Method: Total 30 SD rats were divided into three groups: control, cigarette smoke exposure (CSE), and CSE+simvastatin group, 10 animals in each group. Animals of CSE group and CSE+simvastatin group were exposed to cigarette smoke for 4 months. Lung histopathological alteration was examined by H&E staining. Protein levels of MMP-9, MMP-12 and TIMP-1 in the bronchial alveolar lavage fluid (BALF) as well as in blood circulation were quantified by ELISA. Result: Both BALF and serum protein levels of MMP-9, MMP-12 and TIMP-1 were significantly increased in the animals exposed to cigarette smoke compared to that in control animals (P <0.01). Simvastatin significantly blocked cigarette smoke stimulation on MMP-9, MMP-12 and TIMP-1 release in both BALF and blood circulation (P <0.05), and reduced the ratio of MMP-9 versus TIMP-1 and MMP-12 versus TIMP-1 (P <0.01). Conclusion: Simvastatin inhibited MMP-9 and MMP-12 release in response to cigarette smoke exposure and re-balanced the dis-regulated MMPs versus TIMP-1, and by which mechanism, simvastatin may prevent the development of COPD in response to cigarette smoke insult. © 2015 American Scientific Publishers. Source

Wang X.,Weifang Medical University | Gao S.,Weifang Medical University | Wang S.,Weifang Medical University | Sun H.,Weifang Medical University | And 3 more authors.
2014 2nd International Conference on Systems and Informatics, ICSAI 2014 | Year: 2015

The value of diffusion tensor imaging (DTI) was investigated in the cerebral white matter microstructural abnormalities in patients with first-episode depression. Thirteen patients with first-episode depression and fourteen age- and gender-matched healthy volunteers were performed DTI. The apparent diffusion coefficient (ADC) and fractional anisotropy (FA) of prefrontal lobe deep white matter, the parahippocampal gyrus, the genu, body and splenium of the corpus callosum were recorded. FA and ADC in the deep white matter of frontal lobe, the right parahippocampal gyrus and the genu of the corpus callosum in the depression group were different from those of control group (P<0.05). There were no significant differences of FA and ADC in the left parahippocampal gyrus and the body and splenium of the corpus callosum between two groups (P>0.05). DTI was valuable in detection of microstructural abnormalities of the white matter in patients with first-episode depression. The microstructural abnormalities in the deep white matter of frontal lobe, the right side of the parahippocampal gyrus and genu of the corpus callosum may be the neurobiological basis of first-episode depression. © 2014 IEEE. Source

Liu Y.,Key Laboratory of Endocrinology | Liu H.J.,Peoples Hospital of Liaocheng | Li M.,Key Laboratory of Endocrinology | Zhou P.R.,Key Laboratory of Endocrinology | And 9 more authors.
Chinese Medical Journal | Year: 2014

Background Genetic factors are important in the pathogenesis of osteoporosis, but less is known about the genetic determinants of osteoporosis treatment. We aimed to explore the association between the gene polymorphisms of key enzyme farnesyl diphosphate synthase (FDPS) in mevalonate signaling pathway of osteoclast and response to alendronate therapy in osteoporotic postmenopausal women in China. Methods The study group comprised 639 postmenopausal women aged (62.2±7.0) years with osteoporosis or osteopenia who had been randomly assigned to low dose group (70 mg/2w) or standard dose group (70 mg/w) of alendronate in this 1-year study. We identified allelic variant of the FDPS gene using the polymerase chain reaction and restriction enzyme Faul. Before and after treatment, serum levels of calcium, phosphate, alkaline phosphatase (ALP), cross linked C-telopeptide of type I collagen (β-CTX) were detected. Bone mineral density (BMD) at lumbar spine and proximal femur was measured. The association was analyzed between the polymorphisms of FDPS gene and the changes of BMD, bone turnover biomarkers after the treatment. Results The FDPS rs2297480 polymorphisms were associated with baseline BMD at femoral neck, and patients with CC genotype had significantly higher baseline femoral neck BMD ((733.6±84.1) mg/cm2) than those with AC genotypes ((703.0±86.9) mg/cm2) and AA genotypes ((649.8±62.4) mg/cm2) (P <0.01). No significant difference in BMD at lumbar spine was observed among different genotypes of FDPS. The percentage change of serum ALP level was significantly lower in patients with CC genotype (-22.9%) than that in those with AC genotype (-24.1%) and AA genotype (-29.8%) of FDPS after 12 months of alendronate treatment (P <0.05). Neither percentage change of BMD nor β-CTX level after alendronate treatment had association with FDPS genotype. Conclusions FDPS gene was probably a candidate gene to predict femoral neck BMD at baseline. FDPS gene alleles could predict change percentage of ALP after treatment of alendronate, but possibly had no significant relationship with the responsiveness of BMD to alendronate therapy. Source

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