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Zhang C.,First Peoples Hospital of Foshan | Che J.,Peoples Hospital of Laiwu | Yu J.,First Peoples Hospital of Foshan | Yu L.,First Peoples Hospital of Foshan | And 2 more authors.
PLoS ONE | Year: 2015

This study is designed to evaluate the visual outcomes, accuracy, and predictability of corneal flaps with different thicknesses created by 60-kHz femtosecond laser according to different corneal thicknesses in the patients with low and moderate refractive error. A total of 182 eyes were divided according to the central corneal thickness (470μm-499 μm in Group A, 500μm-549 μm in Group B, and 550μm-599 μm in Group C) and underwent femtosecond laser-assisted LASIK for a target corneal flap thickness (100 μm for Group A, 110 μm for Group B, and 120 μm for Group C). Uncorrected distance visual acuity (UDVA), corrected distance visual acuity (CDVA), and refractive status were examined. The flap thickness of each eye was measured by anterior segment optical coherence tomography (AS-OCT) on 30 points at 1-month follow-up to assess the accuracy and predictability. Postoperatively, at least 75% of eyes had a UDVA of 20/16 or better, less than 2% of eyes lost one line, over 30% of eyes gained one or more lines in CDVA, at least 95% of eyes had astigmatism of less than 0.25 D, all eyes achieved a correction within ±1.00 D from the target spherical equivalent refraction. The visual and refractive outcomes did not differ significantly in all groups (P >0.05). The mean flap thickness was 100.36± 4.32 μm (range: 95-113 μm) in Group A, 111.64 ± 3.62 μm (range: 108-125 μm) in Group B, and 122.32 ±2.88 μm (range: 112-128 μm) in Group C. The difference at each measured point among the three groups was significant (P < 0.05). The accuracy and predictability were satisfactory in all three groups. In conclusion, this customized treatment yielded satisfactory clinical outcomes with accurate and predictable flap thickness for patients with low and moderate refractive error. © 2015 Zhang et al. Source

Deng Y.,Peoples Hospital of Laiwu | Jiang X.-D.,Peoples Hospital of Laiwu | Geng L.,Peoples Hospital of Laiwu
Journal of Practical Oncology | Year: 2010

Objective: To explore neutrophil gelatinase-associated lipocalin (NGAL) expression in epithelial ovarian tumors and its clinical significance. Methods: The NGAL expression was detected in 60 cases of ovarian epithelial cancer, 30 of borderline tumor, 10 of benign adenoma and 10 of normal tissue with immunohistochemistry. Its relationship with clinicopathological features and prognosis was analyzed. Results: The positive rate of NGAL exprssion in epithelial ovarian cancer and ovarian border line tumor was 81.7% and 60.0% , which was significantly higher than that in the benign adenoma and normal tissues (P < 0.05). In epithelial ovarian cancer, NGAL expression was significantly correlated with different histological classifications (P < 0.05), International Federation of Gynecology and obstetrics (FIGO) stage (P < 0.05), and lymph node metastasis (P < 0.05). Conclusions: The positive expression of NGAL is closely correlated with development, invasion and metastasis of epithelial ovarian cancer. NGAL might be a potential biomarker for the early diagnosis and prognosis of epithelial ovarian cancer. Source

Zhao W.,Peoples Hospital of Laiwu | Lu M.,Peoples Hospital of Laiwu | Zhang Q.,Peoples Hospital of Laiwu
Molecular Medicine Reports | Year: 2015

Chloride intracellular channel 1 (CLIC1) has been demonstrated to be overexpressed in gastric cancer, and elevated CLIC1 expression levels are markedly associated with the processes of tumor cell migration and invasion. However, the regulatory mechanism and signaling pathway underlying these processes have remained to be elucidated. The present study examined the impact of N-acetyl cysteine (NAC), indanyloxyacetic acid (IAA)-94 and SB203580, inhibitors of reactive oxygen species (ROS), as well as CLIC1 and p38 mitogen-activated protein kinase (MAPK) on the migration and invasion of SGC-7901 gastric cancer cells in a hypoxia-reoxygenation (H-R) microenvironment. The results demonstrated that intracellular ROS and CLIC1 levels were increased under H-R conditions, and that functional inhibition of CLIC1 significantly decreased the H-R-elevated ROS generation and p-p38 MAPK levels in SGC-7901 cells, as well as inhibited the migration and invasion of SGC-7901 cells. In addition, the expression levels of MMP-2 and MMP-9 were inhibited by NAC, IAA-94 and SB203580. These results indicated that CLIC1 regulates gastric cancer-cell migration and invasion via the ROS-mediated p38 MAPK signaling pathway. Source

Gao Y.,Peoples Hospital of Laiwu | Li P.,Peoples Hospital of Laiwu | Liu Z.,Peoples Hospital of Laiwu | Diao X.,Peoples Hospital of Laiwu | Song C.,Peoples Hospital of Laiwu
Experimental and Therapeutic Medicine | Year: 2015

The present study aimed to investigate the roles of the vascular endothelial cell growth factor (VEGF) and micro (mi)RNA-210 in the metastasis of primary medulloblastoma (MB) tumors. A total of 86 adult patients diagnosed with cerebellar MB were enrolled in the present study, of which 11 patients had metastatic MB in the subarachnoid space. The following samples were collected: MB primary tumor tissue, MB secondary tumor tissue, tumor adjacent tissues and cerebrospinal fluid (CSF). Immunohistochemical analyses of the tissue samples were conducted in order to detect patterns of VEGF expression. In addition, the expression levels of VEGF mRNA and miRNA-210 were analyzed using reverse transcription-quantititative polymerase chain reaction, and western blot analyses were used to investigate VEGF protein expression levels. The positive expression rate of VEGF was significantly higher in MB tumor tissue, as compared with adjacent tissues (P<0.01). In addition, VEGF mRNA and protein expression levels in MB primary and secondary tumor tissues, and in the CSF of patients with metastatic MB, were significantly upregulated, as compared with tumor adjacent tissues and the CSF of patients with non-metastatic MB, respectively (P<0.01). miRNA-210 expression levels were significantly upregulated in MB tumor tissues, the CSF of patients with metastatic MB and in tumor tissues of metastatic MB (P<0.01). In the present study, the expression levels of VEGF and miRNA-210 were upregulated in patients with MB and metastatic MB; thus suggesting that miRNA-210 may promote the metastasis of MB primary tumors by regulating the expression of VEGF. © 2015, Spandidos Publications. All rights reserved. Source

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