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Xiong Y.,Sichuan University | Li H.,Sichuan University | Zhou C.,University of Sichuan | Yang X.,University of Sichuan | And 3 more authors.
Journal of Biomaterials Science, Polymer Edition | Year: 2014

A new type of cervical vertebra cage was prepared using a novel composite, calcium deficient hydroxyapatite/poly(amino acid) (HA/PAA), and its mechanical properties, in vitro stability and bioactivity, and in vivo biocompatibility were characterized. The results showed that the axial compressive loads of the HA/PAA cage were in the range of 10058-10612 N and the lateral compressive loads were in the range of 1180-2363 N, and varied with the height of the cervical vertebra cages. After immersion in simulated body fluid (SBF) for 16 weeks, the axial compressive loads of the cage decreased from 10058 to 7131 N and the lateral compressive loads decreased from 1180 to 479 N. In addition, the weight loss decreased 6.01%, showing that HA/PAA composites had good stability during the incubation period. The pH value of SBF was also monitored during the whole soaking period; it fluctuated in the range of 6.9-7.4. Scanning electron microscope and energy dispersive spectrometer results showed the cage was bioactive with a new apatite layer attached on the surface. The histological evaluation revealed that new bone tissue bonded tightly with the surfaces of the implants, showing excellent biocompatibility. In conclusion, the HA/PAA cage showed sufficient strength, good stability, bioactivity, and biocompatibility, and has potential applications for clinical cervical vertebrae repair. © 2014 © 2014 Taylor & Francis.


Zhang J.,Soochow University of China | Bao J.,Soochow University of China | Wang R.,Guangzhou University | Geng Z.,Soochow University of China | And 8 more authors.
Journal of Hazardous Materials | Year: 2016

This multi-centered study was designed to evaluate the biological effects of exposure to antineoplastic drugs (ADs) at PIVAS (Pharmacy Intravenous Admixture Service) across ten Chinese hospitals. 8-hydroxy-2-deoxyguanosine (8-OHdG) was used as a biomarker of DNA oxidative damage and lymphocyte apoptosis assays using peripheral lymphocyte cells were used to detect primary DNA damage. The mutagenicity activity was estimated with the Ames fluctuation test. 158 exposed and 143 unexposed workers participated in this study. The urinary 8-OHdG/Cr concentrations of the exposed group was 22.05 ± 17.89 ng/mg Cr, which was significantly higher than controls of 17.36 ± 13.50 ng/mg Cr (P < 0.05). The rate of early lymphocyte apoptosis was slightly increased in exposed group than that of the control group (P = 0.087). The mutagenic activity was significantly higher in the exposed group relative to the control group (P < 0.05). Moreover, while no statistically significant difference was observed, higher concentrations of 8-OHdG/Cr in urine and an early lymphocyte apoptosis rate were found in exposed group II as compared to exposed group I. In addition, a significant correlation between early lymphocyte apoptosis and exposure time to ADs was also observed (P < 0.05). In conclusion, our study identified elevated biomarkers in PIVAS workers exposed to ADs. However whether these findings could lead to increased incidence of genotoxic responses remains to be further investigated. © 2016 Elsevier B.V.


Nian W.,Chongqing Tumor Hospital | Nian W.,Chongqing Medical University | Ao X.,Chongqing Medical University | Ao X.,Peoples Hospital of Huangshan | And 7 more authors.
Oncology Letters | Year: 2013

microRNAs (miRNAs) have been hypothesized to function as oncogenes or tumor suppressors by targeting specific cancer-related genes. Previous studies have reported that miR-223 may serve as a tumor suppressor in a number of cancer types, however, knowledge of its targets in non-small cell lung cancer (NSCLC) remains limited. In the current study, miR-223 was found to inhibit cell proliferation in vitro by CCK-8 assay, growth curves and an anchorage-independent growth assay in a Lewis lung carcinoma (LLC) cell line. miR-223 transfection in the LLC cells was observed to significantly inhibit migration and invasion, induce G2/M arrest and decrease the expression levels of Sca-1, a marker of murine stem cells. In addition, miR-223 transfection markedly suppressed AKT and ERK signaling, as well as insulin-like growth factor-1 receptor (IGF-1R)-mediated downstream signaling, pathways that are crucial for cell proliferation and invasion in NSCLC cells. Analyses in C57BL/6 mice demonstrated that miR-223 suppresses tumorigenicity in vivo. Using a luciferase activity assay and western blot analysis, IGF-1R and cyclin-dependent kinase 2 (CDK2) were identified as direct targets of miR-223. In the present study, novel cancer-related targets of miR-223 were identified and verified in a LLC cell line, indicating that miR-223 functions as a tumor suppressor, which may fine-tune the activity of the IGF-1R pathway in lung cancer. Therefore, increasing miR-223 expression may provide a novel approach for the treatment of NSCLC.


Yan Y.,Sichuan University | Xiong Y.,Sichuan University | Li H.,Sichuan University | Zhou C.,University of Sichuan | And 3 more authors.
Journal of Biomaterials Science, Polymer Edition | Year: 2014

A new type of cervical vertebra cage was prepared using a novel composite, calcium deficient hydroxyapatite/poly(amino acid) (HA/PAA), and its mechanical properties, in vitro stability and bioactivity, and in vivo biocompatibility were characterized. The results showed that the axial compressive loads of the HA/PAA cage were in the range of 10058-10612 N and the lateral compressive loads were in the range of 1180-2363 N, and varied with the height of the cervical vertebra cages. After immersion in simulated body fluid (SBF) for 16 weeks, the axial compressive loads of the cage decreased from 10058 to 7131 N and the lateral compressive loads decreased from 1180 to 479 N. In addition, the weight loss decreased 6.01%, showing that HA/PAA composites had good stability during the incubation period. The pH value of SBF was also monitored during the whole soaking period; it fluctuated in the range of 6.9-7.4. Scanning electron microscope and energy dispersive spectrometer results showed the cage was bioactive with a new apatite layer attached on the surface. The histological evaluation revealed that new bone tissue bonded tightly with the surfaces of the implants, showing excellent biocompatibility. In conclusion, the HA/PAA cage showed sufficient strength, good stability, bioactivity, and biocompatibility, and has potential applications for clinical cervical vertebrae repair. © 2014 © 2014 Taylor & Francis.


Nian W.-Q.,Chongqing Medical University | Chen F.-L.,Chongqing Medical University | Ao X.-J.,Peoples Hospital of Huangshan | Chen Z.-T.,Chongqing Medical University
Molecular and Cellular Biochemistry | Year: 2011

There is increasing evidence that cancer stem cells contribute to the initiation and propagation of many tumor. Therefore, to find out and identify the metastatic tumor stem-like cells in Lewis lung cancer cell line (LLC), the expression of CXCR4 was measured in LLC by flow cytometry and observed by laser scanning confocal microscope (LSCM). After the CXCR4 + LLC cell was isolated from LLC by magnetic cell sorting, its properties were evaluated by their tumorigenic and metastatic potentials. CXCR4 + cells were counted for 0.18% of the total number of LLC, and immunofluorescent staining cells were identified by LSCM. CXCR4 + LLC suspension cultured in a serum-free medium, cell spheres expressed a high level of Sca-1. The chemotherapy sensitivity to cisplatin of CXCR4 + LLC was lower than that of CXCR4 - LLC. The expression of ABCG2 and IGF1R mRNA in CXCR4 + LLC was higher than that in CXCR4 - LLC (P < 0.01). Most of CXCR4 + LLC cells were close to vascular endothelial cells, aberrant vasculature around it was forming. The expression of VEGF and MMP9 mRNA in CXCR4 + LLC was higher than that in CXCR4 - LLC (P < 0.05), the microvessel density (MVD) of CXCR4 + subsets growing were higher than that of CXCR4 - subsets growing tumor tissue (P < 0.01). The tumor size, volume, and metastatic foci in the lungs of CXCR4 + LLC was significantly higher than that in CXCR4 - LLC (P < 0.001). Similarly, elevated expression of MMP9 and VEGF was also positively associated with CXCR4 + LLC. Our results demonstrated that CXCR4 + cells from Lewis lung carcinoma cell line exhibit cancer metastatic stem cell characteristics. © 2011 Springer Science+Business Media, LLC.

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