Xiong Y.-B.,Peoples Hospital of Huangpi District
International Eye Science | Year: 2013
AIM: To investigate the ultrastructure of the basal vitreo-retina interface in enucleated pig eyes after vitreous injection of enzymes. METHODS: Totally 130 eyes were obtained from freshly slaughter pigs and divided into 5 groups. Each group was sub-divided into the 15 minutes group and the 30 minutes group, with 13 eyes in each group. The control group were performed middle vitreous injection of 0.1 mL PBS, 200 U/mL and 800 U/mL hyaluronidase(HA) were injected in groups A and B, 10 U/mL and 50 U/mL chondroitinase (CA) in groups C and D. After incubation for 15 minutes and 30 minutes, the eye balls were fixed with 4% glutaraldehyde and retina fixative solution. Pathological examination, eosin-hematoxylin staining, scan electron microscopy were taken to evaluate the remaining vitreous on vitreous base, transmission electron microscopy to evaluate retina toxicity. RESULTS: Both of gross appearance and hematoxylin+eosine slice all revealed basal vitreous partially liquefied and degradation. Remarkably decrease was found in basal vitreous than the control group both in the HA 800 U/mL group and the CA 50 U/mL group when using electron microscopy scanning. Transmission electron microscopy in group B, C and D revealed the remaining vitreous were less than the control group. CONCLUSION: Both CA and HA can cause basal vitreoretinal detachment in enucleated pig eyes. But the HA may cause less damage to the retina. Copyright 2013 by the IJO Press.
Wang P.,Shandong University |
Chen J.,Peoples Hospital of Huangpi District |
Mu L.-H.,Shandong University |
Du Q.-H.,Shandong University |
And 2 more authors.
European Review for Medical and Pharmacological Sciences | Year: 2013
Background and Aim: Propofol is one of the most commonly used intravenous anaesthetic agents during cancer resection surgery. It has recently found that propofol has the effect to inhibit cancer cell migration and invasion and sensitize cancer cells to chemotherapy. However, the role of the propofol on the ovarian cancer cells is unknown. In the present study, we explored the effect of propofol on invasion and chemosensitization of ovarian cancer cells to paclitaxel. Materials and methods: The paclitaxel sensitivity of ovarian cancer cell lines HO- 8910PM, H0-8910, SKOV-3, OVCAR-3, COC1 and ES-2 were determined by MTT assays. The Slug levels in the cell lines and the effects of propofol on Slug levels in the cell lines were determined by western blot assays. The effect of propofol on invasion, migration and paclitaxel-induced ovarian cancer apoptosis was determined by Boyden chamber assays, cell MTT,TUNEL assays. Results: The results showed that the cell lines COC1, H0-8910 and ES-2 were sensitive, whereas HO-8910PM, OVCAR-3, SKOV-3, were resistant to paclitaxel. Significant correlation was observed between basal Slug levels and paclitaxel sensitivity. Paclitaxel treatment increased Slug levels. Treatment with propofol induced apoptosis and increased paclitaxel killing of all paclitaxel-sensitive and -resistant ovarian cancer cells followed by significant decrease in the Slug levels. Treatment with propofol inhibits invasion and migration. Conclusions: These data suggest a new mechanism by which the propofol inhibits invasion and metastasis,enhances paclitaxel-induced ovarian cancer cell apoptosis through suppression of Slug.
Hong-bing H.,Peoples Hospital of Huangpi District
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2011
To study the expression and clinical significance of serum vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in children with acute leukemia (AL). The serum VEGF and bFGF level before chemotherapy from 40 cases of children with newly diagnosed AL, after 1 course of treatment from from 37 cases of children with complete remission (CR) and no- remission(NR) , and 40 cases of healthy children (control group) were detected by ELISA technology. Before treatment, the serum VEGF and bF-GF level of the AL group and different types of AL were significantly higher than the control group(P<0.05), while the serum VEGF and bFGF level were no significant difference between the different types of AL ( P > 0.05). Before treatment, the serum VEGF and bFGF level were no significant difference between the AL CR and NR children; After treatment, the serum VEGF and bFGF level of CR children decreased significantly, compared with the prior-treatment and NR children the different was significant ( P < 0. 05); While the serum VEGF and bFGF level of NR children were no significant difference between prior-treatment and post-treatment. The serum VEGF and bFGF level have high expression in children with AL, the serum VEGF and bFGF level can be used as indicators of AL efficacy, and are correlation with the prognosis of children with AL.
Zhen Y.,Peoples Hospital of Huangpi District
Chinese Journal of Tissue Engineering Research | Year: 2014
BACKGROUND: Large randomized controlled clinical trials have shown that drug-eluting stents has a lower restenosis rate compared with bare-metal stents, but the drug-eluting stents cannot reduce major cardiovascular events and death rates, and long-term follow-up of clinical registries show the drug-eluting stents even may increase late stent thrombosis events. OBJECTIVE: To evaluate the incidence of stenosis and adverse reactions after treatment with biodegradable, non-biodegradable drug-eluting stents and bare-metal stents in patients after myocardial infarction. METHODS: We retrospectively analyzed the clinical data of 236 cases of acute myocardial infarction, in which 79 cases were treated with biodegradable sirolimus-eluting stents, 83 cases treated with non-biodegradable sirolimus-eluting stents, and 74 cases treated with bare metal stents. Then, we compared late lumen loss and restenosis within 12 months after stent implantation and major adverse cardiac reactions within 24 months after stent implantation. RESULTS AND CONCLUSION: After 12 months, the late lumen loss of the bare stent group was significantly higher than that of biodegradable and non-biodegradable sirolimus-eluting stent groups (P < 0.05), but the difference in stent restenosis rate (P > 0.05). After 24 months, there were no differences in death, cardiac death, recurrent myocardial infarction, target vessel revascularization, target lesion revascularization, major adverse cardiac events and stent thrombosis events among the three groups. These three kinds of stents need to be further studied in their long-term efficacy and safety as percutaneous coronary intervention for acute ST-segment elevation myocardial infarction. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All Rights Reserved.
Deng Y.S.,Peoples Hospital of Huangpi District
Zhonghua nan ke xue = National journal of andrology | Year: 2013
To observe the therapeutic effect of Qilin Pills combined with bromocriptine on idiopathic hyperprolactinemic (HPRL) oligoasthenospermia. We conducted a randomized controlled study on 40 cases of idiopathic HPRL oligoasthenospermia, who were equally assigned to a trial group and a control group to be treated with Qilin Pills (6 g tid) combined with bromocriptine and bromocriptine alone, respectively, both for a course of 12 weeks. Then we observed the changes in the semen volume, sperm concentration, sperm motility and the levels of serum prolactin and testosterone, and compared the therapeutic results between the two groups before and after medication. Compared with the parameters before medication, both the trial and the control group showed significant improvement after treatment in sperm concentration ([11.60 +/- 3.90] x 10(6)/ml vs [28.10 +/- 13.50] x 10(6)/ml and [12.03 +/- 4.10] x 10(6)/ml vs [18.85 +/- 8.50] x 10(6)/ml), the percentage of grade a sperm ([8.75 +/- 6.65]% vs [24.35 +/- 13.25 ]% and [8.70 +/- 6.70] % vs [19.65 +/- 10.05]%), the percentage of grade a + b sperm ( [28.45 +/- 11.35]% vs [45.80 +/- 16.55]% and [27.65 +/- 10.65]% vs [35.66 +/-13.25]%), and sperm motility ([38.22 +/- 16.35]% vs [60.05 +/- 20.65]% and [37.25 +/- 15.75 ]% vs [52.65 +/- 18.25 ]%) (all P<0.01). No significant differences were found in semen volume (P>0.05). The serum prolactin levels were significantly decreased in the trial and control groups ([152.00 +/- 22.32] and [160.45 +/- 26.65] mIU/L), as compared with premedication ([482.25 +/- 65.32] and [477.32 +/- 60.25] mIU/L) (P<0.01), while the serum testosterone levels were remarkably higher ([16.35 +/- 5.52] and [11.15 +/- 4.65] nmol/L) than before treatment ([3.75 +/- 1.10] and [4.05 +/- 1.30] nmol/L) (P<0.01). There were no statistically significant differences in the serum prolactin and testosterone levels between the two groups after treatment (P>0.05). Qilin Pills combined with bromocriptine have a significantly better efficacy than bromocriptine alone in the treatment of idiopathic HPRL oligoasthenospermia.
Han J.G.,Capital Medical University |
Wang Z.J.,Capital Medical University |
Qian Q.,Wuhan University |
Dai Y.,Shandong University |
And 4 more authors.
Diseases of the Colon and Rectum | Year: 2014
BACKGROUND: Recent studies have shown that extralevator abdominoperineal resection has the potential for reduced circumferential resection margin involvement, intraoperative bowl perforation, and local recurrence rates; however, it has been suggested that extended resection may be associated with increased morbidity because of the formation of a larger perineal defect. OBJECTIVE: This study was undertaken to demonstrate the feasibility and complications of extralevator abdominoperineal resection for locally advanced low rectal cancer in China. DESIGN: This was a prospective cohort study. SETTING: The study was conducted at 7 university hospitals throughout China. PATIENTS: A total of 102 patients underwent this procedure for primary locally advanced low rectal cancer between August 2008 and October 2011. MAIN OUTCOME MEASURES: The main outcome measures comprised circumferential resection margin involvement, intraoperative perforation, postoperative complications, and local recurrence. RESULTS: The most common complications included sexual dysfunction (40.5%), perineal complications (23.5%), urinary retention (18.6%), and chronic perineal pain (13.7%). Chronic perineal pain was associated with coccygectomy (p < 0.001), and the pain gradually eased over time. Reconstruction of the pelvic floor with biological mesh was associated with a lower rate of perineal dehiscence (p = 0.006) and overall perineal wound complications (p = 0.02) in comparison with primary closure. A positive circumferential margin was demonstrated in 6 (5.9%) patients, and intraoperative perforations occurred in 4 (3.9%) patients. All circumferential margin involvements and intraoperative perforations were located anteriorly. The local recurrence was 4.9% at a median follow-up of 44 months (range, 18-68 months). LIMITATIONS: This was a nonrandomized, uncontrolled study. CONCLUSIONS: Extralevator abdominoperineal resection performed in the prone position for low rectal cancer is a relatively safe approach with acceptable circumferential resection margin involvement, intraoperative perforations, and local recurrences. Reconstruction of the pelvic floor with biological mesh might lower the rate of perineal wound complications. © The ASCRS 2014.
Wang H.-P.,Zhejiang University |
Zhu Y.-L.,Zhejiang University |
Shao W.,Peoples Hospital of Huangpi District
World Journal of Gastroenterology | Year: 2013
Helicobacter pylori (H. pylori) infection might initiate and contribute to the progression of lymphoma from gastric mucosa-associated lymphoid tissue (MALT). Increasing evidence shows that eradication of H. pylori with antibiotic therapy can lead to regression of gastric MALT lymphoma and can result in a 10-year sustained remission. The eradication of H. pylori is the standard care for patients with gastric MALT lymphoma. Cytotoxin-associated gene A (CagA) protein, one of the most extensively studied H. pylori virulence factors, is strongly associated with the gastric MALT lymphoma. CagA possesses polymorphisms according to its C-terminal structure and displays different functions among areas and races. After being translocated into B lymphocytes via type IV secretion system, CagA deregulates intracellular signaling pathways in both tyrosine phosphorylation-dependent and -independent manners and/or some other pathways, and thereby promotes lymphomagenesis. A variety of proteins including p53 and protein tyrosine phosphatases-2 are involved in the malignant transformation induced by CagA. Mucosal inflammation is the foundational mechanism underlying the occurrence and development of gastric MALT lymphoma. © 2013 Baishideng Publishing Group Co., Limited. All rights reserved.
Dong Y.W.,Fudan University |
Wang R.,Fudan University |
Cai Q.Q.,Fudan University |
Qi B.,Fudan University |
And 3 more authors.
Journal of Hepatology | Year: 2014
Background & Aims The biological relevance and regulation mechanism of aberrant miR-223 expression in human hepatocellular carcinoma (HCC) remain unknown. Our aim was to investigate miR-223 regulation in HCC. Methods miR-223 and integrin αV dysregulation were verified in 57 HCC specimens. Immunohistochemical analysis of integrin αV and sulfatide levels was performed on another cohort of 103 HCC samples. Epigenetic analysis was used to explore the effect of sulfatide on miR-223 transcription. Orthotopic growth, and intrahepatic and pulmonary metastasis of tumors derived from SMMC-7721 cells expressing miR-223 or cerebroside sulfotransferase were monitored in mice. Results miR-223 was reduced in HCC specimens and highly metastatic cell lines. Enhanced miR-223 expression had a negative effect on integrin αV-mediated cell migration. In vivo assays of metastasis in an orthotopically implanted model demonstrated that miR-223 effectively inhibited HCC metastasis. Further analysis demonstrated that integrin αV is negatively regulated by miR-223. Moreover, the integrin αV subunit was significantly positively correlated with highly expressed sulfatide in 103 HCC specimens. Intriguingly, miR-223 expression was suppressed by sulfatide in HCC in association with reduced recruitment of acetylated histone H3 and C/EBPα to the pre-miR-223 gene promoter, where monocytic leukemia zinc finger (MOZ) protein, a MYST-type histone acetyltransferase, lost its attachment. The expression of histone deacetylases, HDAC9 and HDAC10, were greatly stimulated by sulfatide and their recruitment to miR-223 gene promoter was enhanced. Conclusions Downregulation of miR-223 in HCC is associated with the epigenetic regulation by highly expressed sulfatide and involved in tumor metastasis. © 2013 European Association for the Study of the Liver. Published.
Zheng C.,Peoples Hospital of Huangpi District
Urologia Internationalis | Year: 2014
Objective: To systematically review the efficacy and safety of retrograde intrarenal surgery (RIRS) versus percutaneous nephrolithotomy (PCNL) for the treatment of renal calculi >2 cm. Methods: We searched PubMed, Cochrane Library, Embase and the Chinese Biomedical Literature Database about RIRS and PCNL for the treatment of renal stones. The retrieval time ended in December 2013. All clinical trials were retrieved and their included references investigated. Two reviewers independently assessed the quality of all included studies, and the eligible studies were included and analyzed using the RevMan 5.2 software. Results: Two randomized controlled trials and six clinical controlled trials were included, involving a total of 590 patients. Our meta-analysis showed that there were not significant differences in stone-free rate (relative risk [RR] = 0.95, 95% confidence interval (CI) 0.88-1.02, p = 0.11) and fever (RR = 0.95, 95% CI 0.54-1.67, p = 0.85) between RIRS and PCNL. We found that hospital stay (weighted mean difference [WMD] = -2.10, 95% CI -3.08 to -1.11, p < 0.10) and bleeding (RR = 0.20, 95% CI 0.06-0.68, p = 0.01) were lower and operation time was longer (WMD = 19.11, 95% CI 7.83-30.39, p < 0.10) for RIRS. Conclusion: RIRS is a safe and effective procedure. It can successfully treat patients with stones >2 cm with a high stone-free rate and significantly reduce hospital stay without increasing complications. RIRS can be used as an alternative treatment to PCNL in selected cases with larger renal stones. However, further randomized trials are needed to confirm these findings. © 2014 S. Karger AG, Basel.
Liao G.,Peoples Hospital of Huangpi District
Chinese Journal of Gastroenterology | Year: 2015
Background: Epigastric pain syndrome (EPS), a subtype of functional dyspepsia, is commonly seen in clinical practice, and severely affects patients' quality of life. Aims: To observe the therapeutic efficacy of flupentixol and melitracen combined with proton pump inhibitor on EPS and its influence on psychological status and sleep quality.Methods: A total of 118 EPS patients from Jan. 2013 to June 2014 at Huangpi District People's Hospital were enrolled, and were randomly divided into observation group and control group. Patients in control group were given esomeprazole 20 mg/d, while patients in observation group were given flupentixol and melitracen 10.5 mg bid in addition to esomeprazole, the treatment course were both for 8 weeks. Psychological status, quality of sleep and clinical therapeutic efficacy were compared between the two groups.Results: Four or 8 weeks after the treatment, anxiety, depression, sleep quality and clinical symptom were significantly ameliorated than before treatment in both two groups (P<0.05), while anxiety, depression and clinical symptom in observation group were significantly better than those in control group (P<0.05). Therapeutic efficacy in observation group was significantly higher than that in control group (94.9% vs. 79.7%, P<0.05). No severe adverse reactions were found in both two groups.Conclusions: Flupentixol and melitracen combined with esomeprazole can effectively ameliorate anxiety and depression, and improve the therapeutic efficacy in EPS patients. COPYRIGHT © 2015 by the Editorial Office of Chinese Journal of Gastroenterology.