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Yao W.,Peking University | Wang C.,Chongqing Medical University | Zhong N.,Guangzhou Institute of Respiratory Disease | Han X.,Peoples Hospital of Hebei Province | And 6 more authors.
Respirology | Year: 2014

Background and objective This study, in a predominantly Chinese population, investigated the efficacy and safety of a once-daily (o.d.) inhaled ultra-long-acting β2-agonist indacaterol for the treatment of moderate-to-severe chronic obstructive pulmonary disease (COPD). Methods This is a 26-week, double-blind study on randomized patients who received indacaterol 150 μg or 300 μg or placebo o.d. The primary variable was trough forced expiratory volume in 1 s (FEV1, average of 23 h 10 min and 23 h 45 min post-dose values) at Week 12. Health status (St George's Respiratory Questionnaire, SGRQ), dyspnoea (transition dyspnoea index, TDI) and safety were evaluated over 26 weeks. Results Of the 563 patients randomized, 561 (89.8% Chinese) received treatment and 482 completed. At Week 12, trough FEV 1 improved significantly for indacaterol 150 and 300 μg versus placebo (1.32, 1.29 vs 1.17; P < 0.001 for both comparisons), with differences exceeding the pre-specified minimal clinically important difference of 0.12 L. At Week 26, TDI score was superior to placebo for indacaterol 150 and 300 μg (0.82, 1.15; P < 0.01), as was the percentage of patients with a clinically relevant improvement (≥1 point) (74.1%, 78.6% vs 55.5%; P < 0.05). Both doses provided ≥4-point improvements from baseline in SGRQ score at Week 26 that were numerically greater than placebo (unadjusted means: -9.6, -8.8 vs -7.0), with a similar pattern in percentage of patients with clinically relevant improvements in SGRQ score (65.0%, 61.5% vs 60.6%). Incidences of adverse events were comparable across treatment groups. Conclusions Indacaterol delivered effective bronchodilation with significant improvements in breathlessness and health status in this predominantly Chinese population. We investigated the effect of indacaterol on FEV1, dyspnoea and health-related quality of life in a predominantly Chinese population diagnosed with moderate-to-severe COPD. Indacaterol proved to be a useful treatment option as it showed effective bronchodilation and improvements in breathlessness and health status in this population. © 2014 The Authors. Respirology © 2014 Asian Pacific Society of Respirology.


PubMed | Bethune International Peace Hospital of PLA, Peoples Hospital of Hebei Province, Beijing Shunyi Hospital, Xijing University and 11 more.
Type: Journal Article | Journal: Rheumatology (Oxford, England) | Year: 2015

To evaluate the impact of RA on work capacity and identify factors related to work capacity impairment in patients with RA.A cross-sectional multicentre study was performed in 21 tertiary care hospitals across China. A consecutive sample of 846 patients with RA was recruited, of which 589 patients of working age at disease onset constituted the study population. Information on the socio-demographic, clinical, working and financial conditions of the patients was collected. Logistic regression analyses were used to identify factors associated with work capacity impairment.The rate of work capacity impairment was 48.0% in RA patients with a mean disease duration of 60 months (interquartile range 14-134 months), including 11.7% leaving the labour force early, 33.6% working reduced hours and 2.7% changing job. Multivariable logistic regression analysis showed that reduced working hours was significantly related to current smoking [odds ratio (OR) 2.07 (95% CI 1.08, 3.97)], no insurance [OR 1.94 (95% CI 1.20, 3.12)], in manual labour [OR 2.66 (95% CI 1.68, 4.20)] and higher HAQ score [OR 2.22 (95% CI 1.36, 3.60)]. There was an association of current smoking [OR 3.75 (95% CI 1.54, 9.15)], in manual labour [OR 2.33 (95% CI 1.17, 4.64)], longer disease duration [OR 1.01 (95% CI 1.00, 1.01)] and lower BMI [OR 0.90 (95% CI 0.82, 0.99)] with leaving the labour force early.There is a substantial impact of RA on the work capacity of patients in China. Social-demographic, disease- and work-related factors are all associated with work capacity impairment.


Hao L.-N.,Peoples Hospital of Hebei Province | Zhang X.-D.,Hebei Province Peoples Hospital | Wang M.,Peoples Hospital of Hebei Province | Yang T.,Hebei Medical University | He S.-Z.,301 Hospital
International Journal of Ophthalmology | Year: 2011

AIM: To explore that if peroxy nitrite induced the expression of inducible nitric oxide synthase (iNOS) via nuclear factor-kappa B (NF-κB) pathway in retinal pigment epithelial (RPE) cells and the antagonism of cholecy stokinin octapeptide-8 (Melatonin, CCK-8) in vitro. METHODS: RPE cells were obtained from eyes of C57 BL/6 mouse and divided into control, peroxy nitrite and CCK-8 groups. Control group was treated with saline, peroxy nitrite group was treated with peroxy nitrite, and CCK-8 group was treated with CCK-8 after added with peroxy nitrite. All changes were observered at 6, 12 and 24 hours after treatment. Gene array analysis, Reverse Transcription Poly merase Chain Reaction (RT-PCR) were used to determ ine the expression of inducible nitric oxide synthase (iNOS) mRNA in RPE cells. Western blotting was used to test the apoptosis of RPE cells. Immunofluorescent staining was used to determ ine the NF-κB pathway signal transduction. RESULTS: Compared to the control group, the expression of iNOS mRNA was up-regulated in peroxy nitrite group and down-regulated in CCK-8 group with gene array analysis. Apoptosis was increased in peroxy nitrite group and decreased in CCK-8 group with western blotting. The NF-κB pathway signal transduction was more and more stronger in the peroxy nitrite group. But in CCK-8 group, little stronger could be observed at 12 hours, then weak at 24 hours with immunofluorescent staining (P<0.001). CONCLUSION: This study suggested that apoptosis of RPE cells was partly induced by peroxy nitrite, which may be the new way of oxidative damage to the RPE cells. The NF-κB signal transduction may affect and reinforce apoptosis mediated by peroxy nitrite. CCK-8 decreased apoptosis of RPE cells induced by peroxy nitrite and is a potential agent for therapy of retinopathy. The mechanism of CCK-8 dealing with RPE cells may be related to its direct inhibition of the form ation of iNOS to produce peroxy nitrite and antagnism of damage of peroxy nitrite to the RPE cells. Copyright International Journal of Ophthalmology Press.


Ning B.,Hebei Medical University | Qi X.,Peoples Hospital of HeBei Province | Li Y.,Peoples Hospital of HeBei Province | Liu H.,Peoples Hospital of HeBei Province | And 2 more authors.
Cellular Physiology and Biochemistry | Year: 2014

Objective: To compare the effects of biventricular electrical pacing and conventional single-ventricular pacing for cardiac contractility modulation (CCM) on cardiac contractile function and to delineate the underlying molecular mechanisms. Methods: Forty rabbits were divided into four groups before surgery: healthy control, HF sham, HF left ventricular pacing CCM (LVP-CCM), and HF biventricular pacing CCM (BVP-CCM) groups with n=10 for each group. A rabbit model of chronic heart failure was established by ligating ascending aortic root of rabbits. Then electrical stimulations during the absolute refractory period were delivered to the anterior wall of left ventricle in the LVP-CCM group and on the anterior wall of both left and right ventricles in the BVP-CCM group lasting six hours per day for seven days. Changes in ventricular structure, cardiac function and electrocardiogram were monitored before and after CCM stimulation. Results: Compared with the sham-operated group, heart weight, heart weight index, LV end-systolic diameter (LVESD), LV end-diastolic diameter (LVEDD) in the LVP-CCM and BVP-CCM groups were significantly decreased (p<0.05), while LV ejection fraction (LVEF) and fractional shortening fraction (FS) were increased (p<0.05). Notably all these changes were consistently found to be greater in BVP-CCM than in LVP-CCM. Moreover, plasma BNP levels were highest in the HF sham-control group, followed by the LVP-CCM group, and lowest in the BVP-CCM group (p<0.05). Furthermore, sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2a) protein levels were upregulated by 1.7 and 2.4 fold, along with simultaneous upregulation of a cardiac-enriched microRNA miR-133 levels by 2.6 and 3.3 fold, in LVP-CCM and BVP-CCM, respectively, compared to sham. Conclusions: Biventricular pacing CCM is superior to conventional monoventricular pacing CCM, producing greater improvement cardiac contractile function. Greater upregulation of SERCA2 and miR-133 may account, at least partially, for the improvement by BVP-CCM. © 2014 S. Karger AG, Basel.


Song S.,Hebei Medical University | Zhang K.,Hebei Medical University | You H.,Hebei Medical University | Wang J.,Hebei Medical University | And 3 more authors.
Clinical and Experimental Immunology | Year: 2010

Summary We have shown that immunization with dendritic cells (DCs) pulsed with hepatitis B virus core antigen virus-like particles (HBc-VLP) packaging with cytosine-guanine dinucleotide (CpG) (HBc-VLP/CpG) alone were able to delay melanoma growth but not able to eradicate the established tumour in mice. We tested whether, by modulating the vaccination approaches and injection times, the anti-tumour activity could be enhanced. We used a B16-HBc melanoma murine model not only to compare the efficacy of DC vaccine immunized via footpads, intravenously or via intratumoral injections in treating melanoma and priming tumour-specific immune responses, but also to observe how DC vaccination could improve the efficacy of adoptively transferred T cells to induce an enhanced anti-tumour immune response. Our results indicate that, although all vaccination approaches were able to protect mice from developing melanoma, only three intratumoral injections of DCs could induce a significant anti-tumour response. Furthermore, the combination of intratumoral DC vaccination and adoptive T cell transfer led to a more robust anti-tumour response than the use of each treatment individually by increasing CD8+ T cells or the ratio of CD8+ T cell/regulatory T cells in the tumour site. Moreover, the combination vaccination induced tumour-specific immune responses that led to tumour regression and protected surviving mice from tumour rechallenge, which is attributed to an increase in CD127-expressing and interferon-γ-producing CD8+ T cells. Taken together, these results indicate that repeated intratumoral DC vaccination not only induces expansion of antigen-specific T cells against tumour-associated antigens in tumour sites, but also leads to elimination of pre-established tumours, supporting this combined approach as a potent strategy for DC-based cancer immunotherapy. © 2010 British Society for Immunology.


Hao L.-N.,Peoples Hospital of Hebei Province | Zhang Y.-Q.,Chengde County Hospital | Shen Y.-H.,Eye Hospital of Handan | Wang Z.-Y.,Chengde County Hospital | And 3 more authors.
International Journal of Ophthalmology | Year: 2010

AIM: To evaluate the peroxy nitrite (ONOO-) of puerarin on retinal pigment epithelial (RPE) cells apoptosis induced partly by peroxy nitrite via Fas/FasL. METHODS: RPE cells from CBL/6 m ice eyes were cultured. Diabetes was induced in Sprague-Dawley (SD) rats by streptozotocin (STZ) intraperitoneal injection. Puerarin was adm inistrated to cultured RPE cells and diabetic rats. Western blotting analysis, DNA ladder, RT-PCR, immuno histochemistry were used for determining the expression of nitroty rosine (NT, the foot print of ONOO-), complement 3 (C; apoptosis and inducible nitricoxide synthase (iNOS) m RNA as well as Fas/FasL signal transduction in RPE cells. RESULTS: Both RPE cells in ONOO- and puerarin group developed apoptosis and expressed NT, C3, iNOS m RNA and Fas/FasL. But latter delayed the all changes in a time-dependent manner compared with control and STZ group (P<0.001 ). iNOS, Cand Fas/FasL were up-regulated and associated with an increase of expression of ONOO- in vivo and in vitro. CONCLUSION: Puerarin decreases RPE cells apoptosis partly induced by ONOO- for diabetic retinopathy. ©International Journal of Ophthalmology Press.


Fan F.,Peoples Hospital of Hebei Province | Li Y.,2nd Peoples Hospital of YunNan Province | Duan X.,Central South University | Zhao T.,Central South University | And 2 more authors.
Graefe's Archive for Clinical and Experimental Ophthalmology | Year: 2012

Purpose To investigate the influence of rosiglitazone on activation of human Tenon's fibroblasts (HTFs) and to access the possible mechanism. Methods Cultured human Tenon's fibroblasts were pretreated in two different concentrations of rosiglitazone (5 μmol/l and 10 μmol/l) before being stimulated with 5 ng/ml transforming growth factor β1 (TGF-β1). The viability and proliferation of cells were accessed by cell count kit-8 assay; Cell migration was examined by the wound closure assay; Alpha smooth muscle actin (α-SMA), connective tissue growth factor (CTGF) and type I collagen (COL I) transcription were detected by RT-qPCR; The expression and localization of α-SMA protein were examined by Western-blot analysis and Immunofluorescence staining; Western-blot analysis was also used to check the expression of CTGF, COL I peroxisome proliferatoractivated receptor gamma (PPAR-γ), and phosphorylation of the signaling protein Smad2/3 Results Rosiglitazone is able to attenuate the up-regulation of α-SMA, CTGF, and COL I transcription, as well as affect protein expression, proliferation, and migration of cells; rosiglitazone also can increase PPAR-γ expression and attenuate Smad2/3 phosphorylation. Conclusions Rosiglitazone can effectively attenuate activation of HTFs induced by TGF-β1 without obvious toxicity. The possible mechanism might be that rosiglitazone interferes with TGF-β/Smad signaling pathway. © Springer-Verlag 2012.

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