Peoples Hospital of Hebei Province
Peoples Hospital of Hebei Province
Wu J.-T.,Jilin University |
Wu J.-T.,Yangzhou University |
Wu H.-Z.,Peoples Hospital of Hebei Province |
Yan C.-G.,Beijing Normal University |
And 4 more authors.
Neuroscience Letters | Year: 2011
Intrinsic brain activity in a resting state incorporates components of the task negative network called default mode network (DMN) and task-positive networks called attentional networks. In the present study, the reciprocal neuronal networks in the elder group were compared with the young group to investigate the differences of the intrinsic brain activity using a method of temporal correlation analysis based on seed regions of posterior cingulate cortex (PCC) and ventromedial prefrontal cortex (vmPFC). We found significant decreased positive correlations and negative correlations with the seeds of PCC and vmPFC in the old group. The decreased coactivations in the DMN network components and their negative networks in the old group may reflect age-related alterations in various brain functions such as attention, motor control and inhibition modulation in cognitive processing. These alterations in the resting state anti-correlative networks could provide neuronal substrates for the aging brain. © 2011 Elsevier Ireland Ltd.
Wang R.-G.,Peoples Hospital Of Hebei Province |
Pi L.-H.,Peoples Hospital Of Hebei Province |
Chen H.-Y.,Peoples Hospital Of Hebei Province |
Zhang H.-Z.,Hebei Medical University
Chinese Journal of Tissue Engineering Research | Year: 2017
BACKGROUND: Neural stem cells have multi-directional differentiation potential, self-sustaining and self-renewal capacity as well as have strong migration ability. Bushen Huoxue Recipe can reduce neuronal damage and promote nerve cell regeneration, to achieve neural function reconstruction. Underlying mechanisms of Bushen Huoxue Recipe combined with neural stem cell transplantation in rats with tinnitus induced by sodium salicylate are yet unclear. OBJECTIVE: To investigate the effects of Bushen Huoxue Recipe combined with neural stem cell transplantation in rats with tinnitus induced by sodium salicylate. METHODS: Sixty Sprague-Dawley rats were randomized into five groups (n=12): normal control group, tinnitus model group, Bushen Huoxue Recipe group, stem cell group and combined treatment group (Bushen Huoxue Recipe combined with neural stem cell transplantation). Animal models of tinnitus induced by sodium salicylate were made in all the groups except for the normal control group. Fifteen days after modeling, rats were given intragastric administration nf Bushen Huoxue Recipe water decoction (3 mL, 2.592 g/mL) for consecutive 7 days in the Bushen Huoxue Recipe group, intravenous injection of neural stem cells (1 mL, 1.0×109/L) in the stem cell group, or their combined treatment in the combined treatment group. RESULTS AND CONCLUSION: Bushen Huoxue Recipe, neural stem cell transplantation and their combination all could effectively promote the recovery of drinking water inhibitory rate that was ranked as follows: combined treatment group < Bushen Huoxue Recipe group < stem cell group. Additionally, the above-mentioned methods could effectively reduce pathological damage of primary auditory cortex in the rat brain. Western blot results showed higher 5-serotonin Idvels in the Bushen Huoxue Recipe group and combined treatment group than in the stem cell group, which were identical to ELISA findings. Our experimental findings indicate that Bushen Huoxue Recipe combined with neural stem cell transplantation exhibit certain effects on tinnitus induced by sodium salicylate in rats, and the mechanism of action is associated with neuronal repair and expression of 5-serotonin at mRNA and protein levels. © 2017, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.
PubMed | Bethune International Peace Hospital of PLA, Peoples Hospital of Hebei Province, Beijing Shunyi Hospital, Xijing University and 11 more.
Type: Journal Article | Journal: Rheumatology (Oxford, England) | Year: 2015
To evaluate the impact of RA on work capacity and identify factors related to work capacity impairment in patients with RA.A cross-sectional multicentre study was performed in 21 tertiary care hospitals across China. A consecutive sample of 846 patients with RA was recruited, of which 589 patients of working age at disease onset constituted the study population. Information on the socio-demographic, clinical, working and financial conditions of the patients was collected. Logistic regression analyses were used to identify factors associated with work capacity impairment.The rate of work capacity impairment was 48.0% in RA patients with a mean disease duration of 60 months (interquartile range 14-134 months), including 11.7% leaving the labour force early, 33.6% working reduced hours and 2.7% changing job. Multivariable logistic regression analysis showed that reduced working hours was significantly related to current smoking [odds ratio (OR) 2.07 (95% CI 1.08, 3.97)], no insurance [OR 1.94 (95% CI 1.20, 3.12)], in manual labour [OR 2.66 (95% CI 1.68, 4.20)] and higher HAQ score [OR 2.22 (95% CI 1.36, 3.60)]. There was an association of current smoking [OR 3.75 (95% CI 1.54, 9.15)], in manual labour [OR 2.33 (95% CI 1.17, 4.64)], longer disease duration [OR 1.01 (95% CI 1.00, 1.01)] and lower BMI [OR 0.90 (95% CI 0.82, 0.99)] with leaving the labour force early.There is a substantial impact of RA on the work capacity of patients in China. Social-demographic, disease- and work-related factors are all associated with work capacity impairment.
Zhang Z.,Peoples Hospital of Hebei Province |
Wang C.,Peoples Hospital of Hebei Province
Chinese Pharmacological Bulletin | Year: 2015
Aim: To observe the expressions of KLF4 in the myocardium of diabetic mice and their changes under Tongxinluo capsule intervention. Methods: Forty KK/Upj-Ay mice were randomly divided into diabetic model group (n: 10) and diabetic model with Tongxinluo (TXL low, middle, high) groups (n: 10, respectively). C57BL/6 mice were selected as control group (n: 10). At the end of the 3 th month the mice were sacrificed and were weighed. The fasting blood-glucose (FBG) glycosylated hemoglobin(HbA1c) triglyceride (TG), total cholesterol (TC) and insulin (FINS) were measured to calculate HOMA-IR. Radioimmunoassay was used to measure serum TNF-α and IL-6. The pathological changes in the myocardium of mice were observed by HE staining. KLF4 mRNA was examined by Real-time PCR, while KLF4 and NF-κB protein were measured by Western blot. Results: Compared to the control group, FBG, HbA1c, TG, TC, FINS, HOMA-IR, TNF-α and IL-6 in model group were markedly increased; the expressions of myocardial KLF4 were markedly decreased and the expression of nuclear NF-κB protein were markedly increased (P < 0.01). Tongxinluo capsule could significantly reduce myocardial pathological damage, FINS, TG, TC, TNF-α, IL-6 level and the expression of nuclear NF-κB protein and up-regulate the expression of KLF4 (P < 0.05), but had no effect on FBG and HbA1c(P > 0.05). Conclusions: KLF4 may be involved in the development of myocardial injury during diabetes. Tongxinluo capsule can ameliorate the myocardial damage and improve the function of diabetic myocardium by up-regulating the expression of KLF4 and inhibiting the activation of NF-κB inflammatory signal pathway.
Ning B.,Hebei Medical University |
Qi X.,Peoples Hospital of HeBei Province |
Li Y.,Peoples Hospital of HeBei Province |
Liu H.,Peoples Hospital of HeBei Province |
And 2 more authors.
Cellular Physiology and Biochemistry | Year: 2014
Objective: To compare the effects of biventricular electrical pacing and conventional single-ventricular pacing for cardiac contractility modulation (CCM) on cardiac contractile function and to delineate the underlying molecular mechanisms. Methods: Forty rabbits were divided into four groups before surgery: healthy control, HF sham, HF left ventricular pacing CCM (LVP-CCM), and HF biventricular pacing CCM (BVP-CCM) groups with n=10 for each group. A rabbit model of chronic heart failure was established by ligating ascending aortic root of rabbits. Then electrical stimulations during the absolute refractory period were delivered to the anterior wall of left ventricle in the LVP-CCM group and on the anterior wall of both left and right ventricles in the BVP-CCM group lasting six hours per day for seven days. Changes in ventricular structure, cardiac function and electrocardiogram were monitored before and after CCM stimulation. Results: Compared with the sham-operated group, heart weight, heart weight index, LV end-systolic diameter (LVESD), LV end-diastolic diameter (LVEDD) in the LVP-CCM and BVP-CCM groups were significantly decreased (p<0.05), while LV ejection fraction (LVEF) and fractional shortening fraction (FS) were increased (p<0.05). Notably all these changes were consistently found to be greater in BVP-CCM than in LVP-CCM. Moreover, plasma BNP levels were highest in the HF sham-control group, followed by the LVP-CCM group, and lowest in the BVP-CCM group (p<0.05). Furthermore, sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2a) protein levels were upregulated by 1.7 and 2.4 fold, along with simultaneous upregulation of a cardiac-enriched microRNA miR-133 levels by 2.6 and 3.3 fold, in LVP-CCM and BVP-CCM, respectively, compared to sham. Conclusions: Biventricular pacing CCM is superior to conventional monoventricular pacing CCM, producing greater improvement cardiac contractile function. Greater upregulation of SERCA2 and miR-133 may account, at least partially, for the improvement by BVP-CCM. © 2014 S. Karger AG, Basel.
Song S.,Hebei Medical University |
Zhang K.,Hebei Medical University |
You H.,Hebei Medical University |
Wang J.,Hebei Medical University |
And 3 more authors.
Clinical and Experimental Immunology | Year: 2010
Summary We have shown that immunization with dendritic cells (DCs) pulsed with hepatitis B virus core antigen virus-like particles (HBc-VLP) packaging with cytosine-guanine dinucleotide (CpG) (HBc-VLP/CpG) alone were able to delay melanoma growth but not able to eradicate the established tumour in mice. We tested whether, by modulating the vaccination approaches and injection times, the anti-tumour activity could be enhanced. We used a B16-HBc melanoma murine model not only to compare the efficacy of DC vaccine immunized via footpads, intravenously or via intratumoral injections in treating melanoma and priming tumour-specific immune responses, but also to observe how DC vaccination could improve the efficacy of adoptively transferred T cells to induce an enhanced anti-tumour immune response. Our results indicate that, although all vaccination approaches were able to protect mice from developing melanoma, only three intratumoral injections of DCs could induce a significant anti-tumour response. Furthermore, the combination of intratumoral DC vaccination and adoptive T cell transfer led to a more robust anti-tumour response than the use of each treatment individually by increasing CD8+ T cells or the ratio of CD8+ T cell/regulatory T cells in the tumour site. Moreover, the combination vaccination induced tumour-specific immune responses that led to tumour regression and protected surviving mice from tumour rechallenge, which is attributed to an increase in CD127-expressing and interferon-γ-producing CD8+ T cells. Taken together, these results indicate that repeated intratumoral DC vaccination not only induces expansion of antigen-specific T cells against tumour-associated antigens in tumour sites, but also leads to elimination of pre-established tumours, supporting this combined approach as a potent strategy for DC-based cancer immunotherapy. © 2010 British Society for Immunology.
Hao L.-N.,Peoples Hospital of Hebei Province |
Zhang Y.-Q.,Chengde County Hospital |
Shen Y.-H.,Eye Hospital of Handan |
Wang Z.-Y.,Chengde County Hospital |
And 3 more authors.
International Journal of Ophthalmology | Year: 2010
AIM: To evaluate the peroxy nitrite (ONOO-) of puerarin on retinal pigment epithelial (RPE) cells apoptosis induced partly by peroxy nitrite via Fas/FasL. METHODS: RPE cells from CBL/6 m ice eyes were cultured. Diabetes was induced in Sprague-Dawley (SD) rats by streptozotocin (STZ) intraperitoneal injection. Puerarin was adm inistrated to cultured RPE cells and diabetic rats. Western blotting analysis, DNA ladder, RT-PCR, immuno histochemistry were used for determining the expression of nitroty rosine (NT, the foot print of ONOO-), complement 3 (C; apoptosis and inducible nitricoxide synthase (iNOS) m RNA as well as Fas/FasL signal transduction in RPE cells. RESULTS: Both RPE cells in ONOO- and puerarin group developed apoptosis and expressed NT, C3, iNOS m RNA and Fas/FasL. But latter delayed the all changes in a time-dependent manner compared with control and STZ group (P<0.001 ). iNOS, Cand Fas/FasL were up-regulated and associated with an increase of expression of ONOO- in vivo and in vitro. CONCLUSION: Puerarin decreases RPE cells apoptosis partly induced by ONOO- for diabetic retinopathy. ©International Journal of Ophthalmology Press.
Fan F.,Peoples Hospital of Hebei Province |
Li Y.,2nd Peoples Hospital of YunNan Province |
Duan X.,Central South University |
Zhao T.,Central South University |
And 2 more authors.
Graefe's Archive for Clinical and Experimental Ophthalmology | Year: 2012
Purpose To investigate the influence of rosiglitazone on activation of human Tenon's fibroblasts (HTFs) and to access the possible mechanism. Methods Cultured human Tenon's fibroblasts were pretreated in two different concentrations of rosiglitazone (5 μmol/l and 10 μmol/l) before being stimulated with 5 ng/ml transforming growth factor β1 (TGF-β1). The viability and proliferation of cells were accessed by cell count kit-8 assay; Cell migration was examined by the wound closure assay; Alpha smooth muscle actin (α-SMA), connective tissue growth factor (CTGF) and type I collagen (COL I) transcription were detected by RT-qPCR; The expression and localization of α-SMA protein were examined by Western-blot analysis and Immunofluorescence staining; Western-blot analysis was also used to check the expression of CTGF, COL I peroxisome proliferatoractivated receptor gamma (PPAR-γ), and phosphorylation of the signaling protein Smad2/3 Results Rosiglitazone is able to attenuate the up-regulation of α-SMA, CTGF, and COL I transcription, as well as affect protein expression, proliferation, and migration of cells; rosiglitazone also can increase PPAR-γ expression and attenuate Smad2/3 phosphorylation. Conclusions Rosiglitazone can effectively attenuate activation of HTFs induced by TGF-β1 without obvious toxicity. The possible mechanism might be that rosiglitazone interferes with TGF-β/Smad signaling pathway. © Springer-Verlag 2012.
He L.-Y.,Peoples Hospital of Hebei Province |
Li L.,Peoples Hospital of Hebei Province |
Guo M.-L.,Peoples Hospital of Hebei Province |
Zhang Y.,Peoples Hospital of Hebei Province |
Zhang H.-Z.,Peoples Hospital of Hebei Province
European Review for Medical and Pharmacological Sciences | Year: 2015
OBJECTIVE: To investigate the relationship between the CD4+CD25+ Treg cell proportion in the peripheral blood and the clinicopathologic features of non-small cell lung cancer (NSCLC) patients and hypoxia-inducible factor-1α (HIF-1α), Ki-67 expression. PATIENTS AND METHODS: Flow cytometry was used to measure the CD4+CD25+ Treg cell level in peripheral blood and immunohistochemical staining used to detect the expression of HIF-1α and Ki-67 protein in cancer tissue of each of 50 NSCLC patients. RESULTS: The level of CD4+CD25+ Treg cell in peripheral blood was related to pathologic grades (t = 3.265, p = 0.006) and clinical stage (t = 4.417, p = 0. 001) of NSCLC instead of to patient's gender and pathologic type of tumor (p 0.05). The level of CD4+CD25+ Treg cell was positively correlated with the expression of HIF-1α (r = 0.711, p = 0.003) and Ki-67 (r = 0.517, p = 0.04), respectively. CONCLUSIONS: CD4+CD25+ Treg cell can be used as a predictor of immune status and prognosis of NSCLC patients and the levels of HIF-1α and Ki-67 protein expression may relate to inhibition of immune cells.