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Xu Q.,Southern Medical University | Pan J.,Peking University | Yu J.,Peoples Hospital of Hainan Province | Liu X.,Southern Medical University | And 6 more authors.
Diabetes Research and Clinical Practice | Year: 2013

Aims: To compare the efficacy and safety of daily lipoic acid (300-600. mg i.v.) plus methylcobalamin (500-1000. mg i.v. or im.) (LA-MC) with that of methylcobalamin alone (MC) on diabetic peripheral neuropathy (DPN). Methods: Electronic database were searched for studies published up to November 1, 2012 and study quality was assessed in duplicate. A random or a fixed effect model was used to analyse outcomes which were expressed as risk ratios (RRs) or mean difference (MD). I2 statistic was used to assess heterogeneity. Results: Seventeen studies were included. Combined data from all studies showed that the LA-MC combination therapy was significantly superior to MC monotherapy (RR = 1.47; 95% CI: 1.37-1.58). Superiority of the LA-MC combination was shown in nerve conduction velocity (NCV) with WMDs of 6.89 (95% CI: 4.24-9.73) for median motor nerve conduction velocity (MNCV), 5.24 (4.14-6.34) for median sensory nerve conduction velocity (SNCV), 4.34 (3.03-5.64) for peroneal MNCV, and 4.53 (3.2-5.85) for peroneal SNCV. There were no serious adverse events associated with treatment. Conclusions: The results of the meta-analysis show that treatment with LA-MC for 2-4. weeks is associated with better outcomes in NCV and neuropathic symptoms relative to MC treatment. However larger well-designed studies are required to confirm this conclusion. © 2013 Elsevier Ireland Ltd.


Cao D.Y.,Peking Union Medical College | Yang J.X.,Peking Union Medical College | Wu X.H.,Fudan University | Chen Y.L.,Cancer hospital of Hunan | And 10 more authors.
British Journal of Cancer | Year: 2013

Background: There are limited data comparing the prognosis and fertility outcomes of the patients with early cervical cancer treated by trans-vaginal radical trachelectomy (VRT) or abdominal radical trachelectomy (ART). The objective of this study was to compare the surgical and pathologic characteristics, the prognosis and fertility outcomes of the patients treated by VRT or ART. Methods: Matched-case study based on a prospectively maintained database of patients underwent radical trachelectomy in 10 centres of China was designed to compare the prognosis and fertility outcomes of the patients treated by VRT or ART. Results: Totally 150 cases, 77 in the VRT and 73 in the ART group, were included. VRT and ART provide similar surgical and pathological outcomes except larger specimens obtained by ART. In the ART group, no patient developed recurrent diseases, but, in the VRT group, 7 (9.8%) patients developed recurrent diseases and 2 (1.6%) patients died of the tumours (P = 0.035). The rate of pregnancy in the VRT group was significantly higher than those of ART (39.5% vs 8.8%; P = 0.003). The patients with tumour size >2 cm showed significant higher recurrent rate (11.6% vs 2.4%, P < 0.05) and lower pregnant rate (12.5% vs 32.1%, P = 0.094) compared with the patients with tumour size <2 cm. Conclusion: Patients treated by ART obtained better oncology results, but their fertility outcomes were unfavourable compared with VRT. Tumour size < 2 cm should be emphasised as an indication for radical trachelectomy for improving the outcome of fertility and prognosis. Copyright © 2013 Cancer Research UK.


Wang T.,Peoples Hospital of Hainan Province | Liao T.-A.,Peoples Hospital of Hainan Province | Zhong S.-B.,Peoples Hospital of Hainan Province
Asian Pacific Journal of Tropical Medicine | Year: 2013

Objective: To study the role of bone marrow mesenchymal stem cells (BMSCs) in construction of vascularized engineered tissue. Methods: hVEGF165 was amplified via RT-PCR before recombinant with pShuttle- green fluorescence protein;green fluorescent protein (GFP)-CMV. Then the recombinant shuttle plasmid was transfected into BMSCs with LipofectamineTM 2000 for packaging and amplifying. hVEGF165 mRNA expression in BMSCs cells was tested. Results: The sequence of hVEGF165 in pShuttle-GFP-hVEGF165 plasmid was confirmed by double-enzyme cleavage method and sequencing. hVEGF165 was highly expressed in BMSCs. Conclusions: The GFP/hVEGF165 recombinant plasmid vector was constructed successfully and expressed effectively in host cells, which may be helpful for discussing the possibility of the application of VEGF165-BMSCs in tissue engineering and ischemic disease cure. © 2013 Hainan Medical College.


Huang L.,Peoples Hospital of Hainan Province | Yao H.-X.,Peoples Hospital of Hainan Province
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology | Year: 2016

OBJECTIVE: To investigate the clinical features of β-thalassaemia intermediate (TI) patients and the curative effect and side reactions of hydroxyurea therapys.METHODS: Twenty nine patients with TI were divided into hydroxyurea therapy group and no hydroxyurea therapy group; the curative effect and side reactions in 2 groups were compared; the situation of blood transfusion in the 2 groups was evaluated.RESULTS: In hydroxyurea therapy group, the hemoglobin level increased after treatment for 3 months; the reticulocyte percentage obviously decreased after treatment for 12 months; the serum ferritin had been maintained at a low level; while in no hydroxyurea therapy group, the levels of hemoglobin and reticulocytes were not significantly improved after treatment, the serum ferritin level gradually increased. In hydroxyurea therapy group, 12 cases were out of blood transfusion after treatment for 12 months, effective rate of treatment was 85.71%; while in no hydroxyurea therapy group, the blood transfusion dependency was not improved after treatment. No serious side reactions were found in all the hydroxyurea treated patients.CONCLUSION: The hydroxyurea shows a better curative effect on TI patients, no serious side reactions occur in all the patients treated with hydroxyurea, but the long-term curative effect and side reactions should be observed continuously.


Guo M.,Peoples Hospital of Hainan Province | Liu T.,Peoples Hospital of Hainan Province | Guo J.-C.,Peoples Hospital of Hainan Province | Jiang X.-L.,Peoples Hospital of Hainan Province | And 2 more authors.
Asian Pacific Journal of Tropical Medicine | Year: 2012

Objective: To evaluate serum IL-2, IL-4, IL-6, IL-8, IL-10 and TNF-α levels in posttraumatic stress disorder (PTSD) patients. Methods: We utilized ELISA technology to examine cytokines such as IL-2, IL-4, IL-6, IL-8, IL-10 and TNF-α in serum from 50 well-characterized individuals with a primary DSM-IV PTSD diagnosis, and 50 age- and gender-matched healthy controls. We conservatively employed a Mann-Whitney U testing. Results: Individuals with primary PTSD had significantly elevated peripheral cytokine levels for all 6 different cytokines compared to age- and gender-matched healthy controls (all P<0.01). Conclusions: These findings suggest that a generalized inflammatory state may be present in individuals with PTSD. © 2012 Hainan Medical College.


Chen F.,Peoples Hospital of Hainan Province | Li J.-J.,Peoples Hospital of Hainan Province | Liu T.,Peoples Hospital of Hainan Province | Wen G.-Q.,Peoples Hospital of Hainan Province | Xiang W.,Peoples Hospital of Hainan Province
Asian Pacific Journal of Tropical Medicine | Year: 2013

Objective: To investigate clinical and neuroimaging features of enterovirus71 (EV71) related acute flaccid paralysis in patients with hand-foot-mouth disease. Methods: Nine patients with acute flaccid paralysis met the criterion of EV71 induced hand-foot-mouth disease underwent spinal and brain MR imaging from May 2008 to Sep 2012. Results: One extremity flaccid was found in four cases (3 with lower limb, 1 with upper limb), two limbs flaccid in three cases (2 with lower limbs, 1 with upper limbs), and four limbs flaccid in two cases. Spinal MRI studies showed lesion with high signal in T2-weighted images (T2WI) and low signal T1-weighted images (T1WI) in the spinal cord of all nine cases, and the lesions were mainly in bilateral and unilateral anterior horn of cervical spinal cord and spinal cord below thoracic 9 (T9) level. In addition, the midbrain, pons, and medulla, which were involved in 3 cases with brainstem encephalitis, demonstrated abnormal signal. Moreover, spinal cord contrast MRI studies showed mild enhancement in corresponding anterior horn of the involved side, and strong enhancement in its ventral root. Conclusions: EV71 related acute flaccid paralysis in patients with hand-foot-mouth disease mainly affected the anterior horn regions and ventral root of cervical spinal cord and spinal cord below T9 level. MR imaging could efficiently show the characteristic pattern and extent of the lesions which correlated well with the clinical features. © 2013 Hainan Medical College.


Chen F.,Peoples Hospital Of Hainan Province | Liu T.,Peoples Hospital Of Hainan Province | Li J.,Peoples Hospital Of Hainan Province | Xing Z.,Peoples Hospital Of Hainan Province | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2014

Objectives: This study aimed to investigate the magnetic resonance imaging (MRI) characteristics and clinical and MRI follow-up findings of patients with neurological complications of enterovirus 71-related hand, foot and mouth disease. Methods: Data were collected from 12 patients who developed neurological complications of enterovirus 71-related hand, foot, and mouth disease during an enterovirus-71 outbreak in Hainan Province, China, from May 2008 to October 2011. Patients were followed up for 2 years. Results: In the six patients with brainstem encephalitis, MRI showed posterior brainstem abnormalities with hyperintense areas on T2-weighted images and hypointense areas on T1-weighted images. In the four patients with acute flaccid paralysis but no brainstem encephalitis, sagittal MRI images showed linear hyperintense areas in the anterior spinal cord, transverse T2-weighted images showed hyperintense areas in the spinal cord, and contrast-enhanced axial T1-weighted images showed strong enhancement of the anterior horns or nerve roots. In the two patients with aseptic meningitis, MRI showed widening of the subarachnoid space and ventricles. The MRI and clinical signs of aseptic meningitis resolved within 4 weeks in both patients. Patients with isolated pontine abnormalities recovered faster than those with multiple brainstem abnormalities, patients with isolated brainstem encephalitis recovered faster than those with associated acute flaccid paralysis, patients with paralysis of one limb recovered faster than those with paralysis of multiple limbs, and patients with isolated thoracolumbar cord abnormalities recovered faster than those with cervical cord abnormalities. Conclusions: MRI is useful for assessment of the neurological complications of enterovirus 71-related hand, foot, and mouth disease. Patients who develop neurological complications characteristically have MRI abnormalities of the posterior brainstem or bilateral anterior horns of parts of the spinal cord. The MRI findings can help to predict prognosis. © 2014 International Journal of Clinical and Experimental Medicine. All rights reserved.


Lu Y.-F.,Peoples Hospital of Hainan Province | Li X.-Q.,Hubei University of Medicine | Han X.-Y.,Peoples Hospital of Hainan Province | Gong X.-G.,Peoples Hospital of Hainan Province | Chang S.-W.,Peoples Hospital of Hainan Province
Asian Pacific Journal of Tropical Medicine | Year: 2013

Objective: To explore peripheral blood cell variations in hepatic cirrhosis portal hypertension patients with hypersplenism. Methods: Clinical data of 322 hypersplenism patients with decreased peripheral blood cells, admitted with cirrhotic portal hypertension, was retrospectively studied over the last 17 years. Results: In 64% (206/322) of patients, more than 2 kinds of blood cell were decreased, including 89 cases of pancytopenia (43.2%), 52 cases of WBC + PLT decrease (25.2%), 29 cases of RBC + PLT decrease (14.1%), and 36 cases of WBC + RBC decrease (17.5%); in 36% (116/322) of patients, single type blood cell decrease occurred, including 31 cases of PLT decrease (26.7%), 29 cases of WBC decrease (25%) and 56 cases of RBC decrease (48.3%). Of 227 routine bone marrow examinations, bone marrow hyperplasia was observed in 118 cases (52.0%), the remainder showed no hyperplasia. For the distinct scope and extent of peripheralblood cell decreases, preoperative blood component transfusions were carried out, then treated by surgery, after whole group splenectomy, the peripheral blood cell count was significantly higher (P<0.05). Conclusions: Of portal hypertensive patients with splenomegaly and hypersplenism, 64% have simultaneous decrease in various blood cells, 36% have decrease in single type blood cells, 52% of patients have bone marrow hyperplasia. A splenectomy can significantly increase the reduction of peripheral blood cells. © 2013 Hainan Medical College.


Shi L.,Capital Medical University | Yang A.-C.,Capital Medical University | Li J.-J.,Capital Medical University | Li J.-J.,Peoples Hospital of Hainan Province | And 3 more authors.
Experimental Neurology | Year: 2015

Anterior thalamic nuclei (ATN) stimulation has been shown to be effective in seizure reduction. Nevertheless, the underlying mechanisms remain unclear. This study investigated the changes in the amino acid levels during chronic, single-sided ATN-stimulation in the hippocampi of rhesus monkeys with mesial temporal lobe epilepsy induced by kainic acid (KA). The concentrations of glutamate, γ-aminobutyric acid, aspartate and taurine in the dialysates from bilateral hippocampi were determined at multiple time points using high-performance liquid chromatography. The results showed that after KA administration, the aspartate, γ-aminobutyric acid and taurine levels increased significantly in the sham-stimulation group, although the γ-aminobutyric acid and taurine levels gradually returned to the basal levels in the chronic stage. The glutamate level showed an initial decrease in the acute stage and a subsequent increase in the chronic stage. Chronic ATN-stimulation reversed the increases in the glutamate and aspartate levels, and maintained the initial increases in the γ-aminobutyric acid and taurine levels till the end of the experiment. These amino acid levels, however, were not affected by either contralateral KA injection or contralateral ATN-stimulation, suggesting that the observed effects of ATN-stimulation are restricted to the ipsilateral hemisphere. Our data suggest that chronic ATN-stimulation may induce favorable modulations in the amino acid levels in the hippocampi of epileptic monkeys, which may be an important mechanism underlying the effects of ATN-stimulation in epilepsy treatment. © 2015 Elsevier Inc.


Chen F.,Peoples Hospital of Hainan Province | Li J.,Peoples Hospital of Hainan Province | Liu T.,Peoples Hospital of Hainan Province | Wang L.,Peoples Hospital of Hainan Province | Li Y.,Peoples Hospital of Hainan Province
European Journal of Paediatric Neurology | Year: 2013

The MRI characteristics of 21 HFMD patients with brainstem encephalitis resulting from EV71 infection were examined to identify lesion patterns helpful in disease classification and prognosis. The author reviewed the clinical and MRI data of 21 children with brainstem encephalitis infected during the EV71 outbreak in Hainan, China from May 2008 to September 2010. Thirteen cases of brainstem encephalitis were classified as type I based on unilateral or bilateral symmetrical patch-like hyperintense T1 and T2 MRI signals restricted to the posterior brainstem. In a significant minority of these cases (6/13), damage to the spinal ventral horn was also found. Among these 13 type I cases, 2 patients died, 7 recovered fully, and 4 suffered from various neurological sequelae. Eight cases were classified with type II brainstem encephalitis based on a vague, speckled hyperintense T1 and T2 signal pattern in the posterior brainstem. Six of these patients recovered fully, and 2 cases suffered from mild sequelae. Reexamination by MRI revealed an enduring lesion in only one type II case, restricted to the medulla oblongata. The prognosis of type II cases was better than that of type I cases. The lesion pattern revealed by MRI can distinguish type I from type II brainstem encephalitis due to EV71 infection and may prove valuable for prognosis. While lesions were usually located in the tegmental part of the brainstem in both patient groups, type I cases also demonstrated spinal, thalamic, and cortical lesions. © 2013 European Paediatric Neurology Society. Published by Elsevier Ltd. All rights.

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