Li M.-Y.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
Li M.-Y.,Changsha Hospital for Maternal and Child Health Care |
Hu X.-X.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
Zhong J.-H.,Guangxi Medical University |
And 2 more authors.
Oncology Letters | Year: 2016
The purpose of the current review was to examine whether systematic lymphadenectomy is safe and effective for treating early-stage endometrial cancer. PubMed, Embase, the Cochrane Library and the China National Knowledge Infrastructure databases were systematically searched during April 2014 to identify studies comparing the use of systematic lymphadenectomy and no systematic lymphadenectomy in parallel for the treatment of early-stage endometrial cancer. A total of 13 eligible studies involving 51,155 patients were included in this review. The median overall survival (OS) rate at 5 years following lymphadenectomy was 90% (range, 73.1-98.3%) for patients undergoing the systematic procedure and 88.2% (range, 68-98.4%) for patients not undergoing the systematic procedure. For the two types of lymphadenectomy, OS has tended to improve over the last 20 years. The combined rate of disease-free and progression-free survival was higher in patients who underwent systematic lymphadenectomy, and the recurrence rate was lower. In particular, systematic lymphadenectomy was associated with markedly higher OS than the non-systematic procedure for patients with intermediate- and high-risk endometrial cancer when ≥11 lymph nodes were removed. Systematic lymphadenectomy demonstrates clinical benefit in patients with early-stage endometrial cancer and should thus be a standard treatment option. In conclusion, systematic lymphadenectomy leads to higher OS than no systematic lymphadenectomy in intermediate- and high-risk patients with early-stage endometrial cancer, particularly when the procedure removes ≥11 lymph nodes. © 2016, Oncology Letters. All Rights Reserved.
Wu F.,U.S. Center for Disease Control and Prevention |
Chi Y.,Peoples Hospital Of Guangxi Zhuang Autonomous Region
Industrial Health | Year: 2015
With the explosive economic growth and social development, China’s regulatory system of occupational health and safety now faces more and more challenges. This article reviews the history of regulatory system of occupational health and safety in China, as well as the current reform of this regulatory system in the country. Comprehensive, a range of laws, regulations and standards that promulgated by Chinese government, duties and responsibilities of the regulatory departments are described. Problems of current regulatory system, the ongoing adjustments and changes for modifying and improving regulatory system are discussed. The aim of reform and the incentives to drive forward more health and safety conditions in workplaces are also outlined. © 2015 National Institute of Occupational Safety and Health.
Hu R.-T.,Guangxi Medical University |
Liu J.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
Zhou Y.,Minerva Foundation Institute for Medical Research |
Hu B.-L.,Guangxi Medical University
Pakistan Journal of Medical Sciences | Year: 2015
Background and Objective: The association between smoking and clinical outcomes after coronary stenting is controversial. The aim of this meta-analysis was to assess the association between smoking and in stent restenosis (ISR), major adverse cardiac events (MACE), or major adverse cardiac and cerebrovascular events (MACCE) after coronary stenting. Methods: A search for studies published before December 2014 was conducted in PubMed, Embase, and Cochrane library. An inverse random weighted meta-analysis was conducted using logarithm of the odds ratio (OR) and its standard error for each study. Results: Ten studies investigated the association between smoking and ISR. Overall, smoking was not associated with ISR (OR: 1.05, 95% CI: 0.79–1.41; I2 = 47.8%). Subgroup analysis also failed to show a significant association between smoking and ISR risk regardless of bare metal stent (BMS) and drug-eluting stent (DES) implantation. Eight studies explored the association between smoking and MACE, but no association was found (OR: 0.92, 95% CI: 0.77–1.10; I2 = 25.5%), and subgroup analysis revealed that no distinct difference was found between BMS and DES implantation. Three studies investigated the association between smoking and MACCE and significant association was found (OR: 2.09, 95% CI: 1.43–3.06; I2 = 21.6%). Conclusions: Our results suggest that in patients undergoing percutaneous coronary intervention with stent implantation, smoking is not associated with ISR and MACE; however, smoking is an independent risk factor for MACCE. © 2015, Professional Medical Publications. All rights reserved.
Wei S.,Guangxi Medical University |
Wang Y.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
Xu H.,Guangxi Medical University |
Kuang Y.,Guangxi Medical University
Oncology Letters | Year: 2015
The aim of the present study was to screen out the biomarkers associated with chemoresistance in ovarian carcinomas and to investigate the molecular mechanisms. microRNA (miRNA) expression data was obtained from published microarray data of the GSE43867 dataset from Gene Expression Omnibus (GEO), including the data of 86 chemotherapy‑treated patients with serous epithelial ovarian carcinomas (response group, 36 complete response cases and 12 partial response cases; non‑response group, 10 stable cases and 28 progressive disease cases), and identification of differentially‑expressed miRNAs were conducted with a GEO2R online tool based on R language. TargetScan 6.2 was used to predict the targets of differentially‑expressed miRNAs. Protein‑protein interaction network analysis was conducted by STRING 9.1, while functional enrichment [Gene Ontology (GO) biological process terms] and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted by GeneCodis3 for the target genes. A total of 6 differentially‑expressed miRNAs were screened out, with 317 target genes obtained. It was found that 67 interactions existed among 76 genes/proteins through the PPI network analysis, and that 6 of these were potential key genes (PIK3R5, MAPK3, PTEN, S1PR3, BDKRB2 and NCBP2). The main biological processes involved in chemoresistant ovarian carcinoma were apoptosis, programmed cell death, cell migration, cell death and cell motility. The miRNA target genes were found to be associated with the ErbB signaling pathway, the gonadotropin‑releasing hormone signaling pathway and other pathways in cancer. IK3R5, MAPK3 and PIK3R5 are involved in the majority of GO terms and KEGG pathways associated with chemoresistance in ovarian carcinoma. © 2015 Spandidos Publications. All rights reserved.
Chen L.,Guangxi Medical University |
Ye H.-L.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
Zhang G.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
Yao W.-M.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
And 2 more authors.
World Chinese Journal of Digestology | Year: 2015
AIM: To investigate the changes of autophagy in hepatocellular carcinoma (HCC) HepG2 cells in response to (-)-epigallocatechin-3-Ogallate (EGCG), and to explore its impact on cell proliferation and death. METHODS: HepG2 cells were routinely cultured and re-plated in Dulbecco's modified eagle's medium (DMEM) in the presence of EGCG of different concentrations. Transmission electron microscopic technique was used to record the formation of autophagosomes in HepG2 cells. Real-time RT-PCR and Western blot were used to detect the mRNA and protein expression of autophagy-related genes, respectively. MTT and trypan blue assays were carried out to determine the cellular proliferation and death. Autophagic intervention experiment was performed to evaluate whether changes in autophagy are involved in the anti-cancer efficacy of EGCG in HCC. RESULTS: The proliferation of HepG2 cells was significantly inhibited by EGCG and was negatively related to the concentrations of this compound (r = -0.9341, P < 0.001). Doses of EGCG that could effectively inhibit the proliferation of HepG2 cells significantly decreased the mRNA and protein expression of Beclin1 and Atg5, with increments of P62 named autophagic substrate as well as substantially reduced numbers of autophagasomes found in these cells. Moreover, up-regulating autophagy with rapamycin was found to apparently impair the effect of EGCG in killing HepG2 cells (t = 9.95, P < 0.01), while 3-MA, an autophagy inhibitor, dramatically exaggerated the anti-cancer effects of EGCG (t = 22.82, P < 0.01). CONCLUSION: EGCG substantially inhibits cell proliferation and promotes cell death in HCC cells via down-regulation of autophagy, which indicates a novel critical pharmacological mechanism of EGCG for hepatoma therapy. © 2015 Baishideng Publishing Group Inc. All rights reserved.
Hu G.,Guangzhou University |
Wu Y.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
Zhou Y.,Guangzhou University |
Yu Y.,Guangzhou University |
And 2 more authors.
Respiratory Care | Year: 2016
BACKGROUND: COPD is associated with cardiovascular and renal dysfunction. Cystatin C (CysC) is a biomarker of renal function and an independent risk factor for all-cause and cardiovascular mortality among elderly persons. The aim of the study was to examine the prognostic role of CysC for in-hospital mortality in subjects with a COPD exacerbation. METHODS: Upon admission, serum CysC levels and arterial blood gas analysis from 477 subjects with a COPD exacerbation were measured. Clinical characteristics were also recorded. A receiver operating characteristic curve analysis was used to determine the level of CysC that discriminated survivors from nonsurvivors. Univariate and multiple logistic regression analyses were used to identify the risk factors for in-hospital mortality. To reduce the influence of confounders, subgroup analyses were performed according to the comorbidities, including states of heart failure, renal dysfunction, and pH, PaCO2, and PaO2 levels. RESULTS: During the in-hospital period, 59 subjects died, and 418 subjects recovered. The decedent group showed lower pH (7.27 ± 0.17 vs 7.38 ± 0.06, P < .001), higher CysC (2.21 ± 1.05 mg/L vs 1.39 ± 0.54 mg/L, P < .001), higher PaCO2 (77 ± 39 mm Hg vs 48 ± 14 mm Hg, P < .001), and lower PaO2 (74 ± 32 mm Hg vs 84 ± 26 mm Hg, P < .001) levels. The area under the receiver operating characteristic curve for the CysC prediction of death was 0.77 (95% CI 0.70–0.84). CysC values ≥1.59 mg/L were associated with significantly higher inhospital mortality (relative risk 2 5.49, 95% CI 3.24 –9.32, P < .001). Multiple logistic regression analysis showed that pH <7.20, CysC ≥1.59 mg/L, and heart failure were independent predictors of in-hospital mortality. The subgroup analysis showed that the comorbid states of renal dysfunction, congestive heart failure, and the levels of pH, PaCO2, and PaO2 did not alter the conclusion that CysC was a mortality risk factor for subjects with a COPD exacerbation. CONCLUSION: CysC was a strong and independent risk factor for hospital mortality secondary to COPD exacerbation. © 2016 Daedalus Enterprises.
Wang X.-T.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
Li D.-G.,Guangxi University |
Li L.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
Kong F.-B.,Guangxi University |
And 2 more authors.
Pathology and Oncology Research | Year: 2015
In lower rectal cancer, postoperative outcome is still subject of controversy between the advocates of abdominoperineal resection (APR) and low anterior resection (LAR). Reports suggest that low anterior resection may be oncologically superior to abdominoperineal excision, although no good evidence exists to support this. Publications were identified which assessed the differences comparing 5-year survival, local recurrence, circumferential resection margin rate, complications and so on. A meta-analysis was performed to clarify the safety and feasibility of the two procedures with several types of outcome measures. A total of 13 studies met the inclusion criteria, and comprised 6,850 cases. Analysis of these data showed that LAR group was highly correlated with 5-year survival (pooled OR = 1.73, 95%CI: 1.30–2.29, P = 0.0002 random-effect). And local recurrence rate of APR group was significantly higher than that in LAR group (pooled OR = 0.63, 95%CI: 0.53–0.75, P < 0.00001 fixed-effect). Also, the circumferential resection margin (CRM) were high involved in APR group than in LAR group. (5 trials reported the data, pooled OR = 0.43, 95%CI: 0.36–0.52, P < 0.00001 fixed-effect). Besides, the incidents of overall complications of APR group was higher compared with LAR group (pooled OR = 0.52, 95%CI: 0.29–0.92, P = 0.03 random-effect). Patients treated by APR have a higher rate of CRM involvement, a higher local recurrence, and poorer prognosis than LAR. And there is evidence that in selected low rectal cancer patients, LAR can be used safely with a better oncological outcome than APR. due to the inherent limitations of the present study, for example, the trails available for this systematic review are limited and the finite retrospective data, future prospective randomized controlled trials will be useful to fully investigate these outcome measures and to confirm this conclusion. © 2014, The Author(s).
Tang Y.-T.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
Yu N.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
Shen Y.,Peoples Hospital Of Guangxi Zhuang Autonomous Region
World Chinese Journal of Digestology | Year: 2016
Aim: To evaluate the clinical effects of early bedside blood filtration in the treatment of acute severe pancreatitis. Methods: Fifty-four patients with acute severe pancreatitis treated in People’s Hospital of Guangxi Zhuang Autonomous Region from October 2013 to October 2015 were randomly divided into either a control group or an observation group, with 27 patients in each group. The control group received conventional therapy, and the observation group received early bedside blood filtration on the basis of conventional therapy. Therapeutic effects were compared between the two groups. Results: After treatment, heart rate, respiratory rate, APACHE II score, interleukin-6, tumor necrosis factor-α, and C reactive protein significantly decreased while oxygenation index significantly increased in the two groups (P < 0.05). Heart rate, respiratory rate, APACHE II score, interleukin-6, tumor necrosis factor-α, and C reactive protein in the observation group were significantly lower than those in the control group (P < 0.05). Oxygenation index in the observation group was significantly higher than that in the control group (P < 0.05). Times to disappearance of abdominal pain, abdominal distension, and peritoneal irritation in the observation group were earlier than those in the control group (P < 0.05). Hospitalization time in the observation group was shorter than that in the control group (P < 0.05). Conclusion: Early bedside blood filtration has a significant effect in the treatment of acute severe pancreatitis, which is helpful to improve the prognosis of patients. © 2016 Baishideng Publishing Group Inc. All rights reserved.
Wu Y.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
Xu F.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
Huang H.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
Chen L.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
And 7 more authors.
Journal of Molecular Histology | Year: 2014
Cell cycle re-entry is one of the key processes in neuronal apoptosis. Previous studies have shown that Ski-interacting protein (SKIP) played an important role in cell cycle re-entry. However, its expression and function in optic nerve injury are still with limited acquaintance. To investigate whether SKIP is involved in retinal ganglion cells (RGCs) death, we performed an optic nerve crush (ONC) model in adult rats. Western blot analysis revealed that up-regulation of SKIP was present in retina at 5 days after ONC. Immunofluorescent labeling indicated that up-regulated SKIP was found mainly in RGCs. We also investigated co-localization of SKIP with active-caspase-3 and TUNEL (apoptotic markers) -positive cells in the retina after ONC. In addition, the expression of SKIP was increased in parallel with P53 and P21 in retina after ONC. All these results suggested that up-regulation of SKIP in the retina was associated with RGCs death after ONC. © 2014, Springer Science+Business Media Dordrecht.
Li M.-Y.,Peoples Hospital Of Guangxi Zhuang Autonomous Region |
Hu X.-X.,Peoples Hospital Of Guangxi Zhuang Autonomous Region
Medical Oncology | Year: 2015
Cervical cancer is one of the most common malignant tumors in women, and numerous studies have associated the disease with changes in microRNA (miRNA) expression. This meta-analysis aimed to consolidate and assess the results of these studies in order to identify potential miRNA biomarkers of cervical cancer. We systematically searched the literature for studies comparing miRNA expression between cervical cancer tissues and normal cervical tissues, and we meta-analyzed the result of 27 studies comprising 1,132 cancer samples and 943 normal samples. We used a vote-counting strategy that took into account total sample and mean fold-change, in order to comprehensively assess associations between certain miRNAs and cervical cancer occurrence and progression. The studies described 195 miRNAs that were significantly up-regulated and 96 microRNAs that were down-regulated in cervical cancer tissues (stage I–IV) relative to normal cervical tissues. Vote-counting analysis showed that up-regulation was most consistently reported for miR-20a and miR-21 (four studies), followed by miR-10a, miR-15b, miR-20b, miR-141, miR-200a, and miR-224 (three studies). Down-regulation was reported most consistently for miR-143 (seven studies), followed by miR-203 and miR-145 (six studies). Fourteen miRNA, respectively, showed a significantly correlated lymphatic node metastasis in eight studies. This meta-analysis has identified several miRNAs whose expression correlates reliably with cervical cancer. These should be probed in further studies to explore their potential as diagnostic biomarkers. © 2015, Springer Science+Business Media New York.