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Wu M.,Fujian Normal University | Wang Q.,Fujian Normal University | Liu X.,Peoples Hospital of Gansu Province | Liu H.,Fujian Normal University
Carbon | Year: 2013

Three dimensional electrospun carbon nanofiber (CNF)/hydroxyapatite (HAp) composites were biomimetically synthesized in simulated body fluid (SBF). The CNFs with diameter of ∼250 nm were first fabricated from electrospun polyacrylonitrile precursor nanofibers by stabilization at 280 °C for 2 h, followed by carbonization at 1200 °C. The morphology, structure and water contact angle (WCA) of the CNFs and CNF/HAp composites were characterized. The pristine CNFs were hydrophobic with a WCA of 139.6°, resulting in the HAp growth only on the very outer layer fibers of the CNF mat. Treatment in NaOH aq. solutions introduced carboxylic groups onto the CNFs surfaces, and hence making the CNFs hydrophilic. In the SBF, the surface activated CNFs bonded with Ca 2+ to form nuclei, which then easily induced the growth of HAp crystals on the CNFs throughout the CNF mat. The fracture strength of the CNF/HAp composite with a CNF content of 41.3% reached 67.3 MPa. Such CNF/HAp composites with strong interfacial bondings and high mechanical strength can be potentially useful in the field of bone tissue engineering. © 2012 Published by Elsevier Ltd.


Ma L.,Guangzhou University | Deng X.,Guangzhou University | Wu M.,Guangdong Medical College | Zhang G.,Peoples Hospital of Gansu Province | Huang J.,Guangzhou University
FEBS Letters | Year: 2014

Nasopharayngeal carcinoma (NPC) is an Epstein-Barr virus (EBV)-associated malignancy. It is known that microRNAs are implicated in the progression of NPC. However, the role of miR-204 in NPC is poorly understood. In this study, we found that miR-204 was down-regulated in NPC cells and tissues. Low-level expression of miR-204 was significantly associated with a more aggressive and poor prognostic phenotype of NPC. We further found that EBV-encoded latent membrane protein 1 (LMP-1) suppressed miR-204 expression by activating Stat-3. Cdc42 was identified as a direct target of miR-204. Mir-204 inhibited EBV positive C666-1 cell invasion and metastasis partly through targeting cdc42. © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.


Zhang X.,Peoples Hospital of Gansu Province
The Journal of international medical research | Year: 2012

To evaluate markers of infection and identify risk factors for the development of pneumonia following acute ischaemic stroke in patients with diabetes mellitus. Patients with diabetes mellitus (n = 106) who were hospitalized after acute ischaemic stroke with (n = 32) or without (n = 74) pneumonia at admission were included in the study. Levels of C-reactive protein (CRP) and interleukin-6 (IL-6), white blood cell (WBC) count, mean body temperature and severity of stroke on the National Institutes of Health Stroke Scale were measured at the time of admission. The degree of disability according to the modified Rankin Scale was assessed after 30 days. Raised levels of IL-6 and CRP, older age, more severe stroke, longer duration of hospitalization and dysphagia were significantly associated with the development of pneumonia. Patients with pneumonia had significantly worse outcomes compared with nonpneumonia patients after 1 month. Raised WBC count and mean body temperature were not significant predictors of pneumonia. Markers of infection, more severe ischaemic stroke, dysphagia and older age may help in predicting the occurrence of pneumonia at stroke onset.


Hu M.L.,Peoples Hospital of Gansu Province
Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2011

To observe the theraputic effect of warming-promotion acupuncture for lumbar muscle strain. Sixty-four cases were randomly divided into a warming-promotion acupuncture group(group A) and a routine acupuncture group (group B), 32 cases in each group. The group A was treated with acupuncture at Shenshu (BL 23) and Guanyuanshu (BL 26) with warming-promotion acupuncture method, (Guanyuanshu (BL 26), Yaoyangguan (GV 3), Weizhong (BL 40) with twirling uniform reinforcing-reducing method. The group B was treated with acupuncture at same acupoints only with twirling uniform reinforcing-reducing method. After four-session treatment, the theraputic effect of two groups was compared. The cured and markedly effective rate of 65.6% (21/32) in the group A, which was superior to that of 40.6% (13/32)in the group B (P < 0.05). The efficiency on lumbar muscle strain treated with warming-promotion acupuncture is superior to that with routine acupuncture, means a suitable manipulation can enhance the therapeutic effect.


Shi Z.F.,Peoples Hospital of Gansu Province
Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine | Year: 2011

With the increase of troops entering the plateau for a variety of missions, the occurrence of de-adaptation increased significantly when the army returned to the plains, however, until now, there has been no effective treatment for de-adaptation to high altitude. To observe the interventional effects of compound Chinese herbal preparations (Sankang Capsule, Rhodiola Rosea Capsule and Shenqi Pollen Capsule) on de-adaptation to high altitude, and provide scientific evidence for appropriate treatment methods in the army health care for future missions. DESIGN, SETTING, PARTICIPANTS AND INTERVENTIONS: A randomized, single-blind, placebo-controlled trial design was used. Soldiers of a returning army unit who exhibited de-adaptation response symptoms were selected for observation after participating in earthquake relief at high altitude. A total of 543 soldiers were divided into a Sankang Capsule group, a Rhodiola Rosea Capsule group, a Shenqi Pollen Capsule group and a placebo group for drug intervention and administered with corresponding drugs. The course of treatment was 15 days. A self-evaluation scale for de-adaptation to high altitude was used to measure the signs and symptoms exhibited by the soldiers. Main outcome measures: Effective rate of signs and symptoms of de-adaptation to high altitude was analyzed after a 15-day treatment and the differences of improvement rate of symptoms between groups were compared to evaluate the efficacy of the drugs. All three drugs improved the symptoms of de-adaptation to high altitude. Compared with the placebo group, symptoms of de-adaptation to high altitude in the drug-treated groups were remitted (P<0.05). Compared with placebo, Sankang Capsule mainly had well-marked effects on dizziness, fatigue, palpitations, cough, sputum and sore throat (P<0.05); Rhodiola Rosea Capsule significantly reduced the symptoms of fatigue, drowsiness, chest tightness, palpitations, vertigo, lack of attention and memory loss (P<0.05); Shenqi Pollen Capsule significantly reduced the symptoms of dizziness, fatigue, weakness, chest tightness, palpitations, cough, sputum, sore throat, memory loss, unresponsiveness and limb numbness (P<0.05). The symptom improvement rate of Shenqi Pollen Capsule was significantly higher than those of the other two drugs. All the three drugs played an evident role in ameliorating symptoms of de-adaptation, and the use of Shenqi Pollen Capsule was more effective than Rhodiola Rosea Capsule and Sankang Capsule.


Wei P.D.,Peoples Hospital of Gansu Province
Zhongguo gu shang = China journal of orthopaedics and traumatology | Year: 2012

To systematically assess the clinical efficacy of bone morphogenetic proteins in the treatment of open tibial fractures. Based on the principles and methods of Cochrane systematic reviews, the Cochrane Library, PubMed, EMBASE, Chinese Bio-medicine Database, China Journal Full-text Database, VIP database were searched from their establishment to April 2012 in whatever language. Related journals were handsearched as well. Randomized controlled trials (RCTs) of bone morphogenetic protein for the treatment of open tibial fractures were included. The quality of the included trials according to the Cochrane Handbook for Systematic Reviews of Interventions Version was assessed. The Cochrane Collaboration's software RevMan 5.1 was used for meta-analysis. Three RCTs totaling 851 patients were included. The results showed that bone morphogenetic protein had no significant differences in fracture healing [RR = 1.16, 95% CI (0.95,1.41), P = 0.15], but lower secondary interventions incidence rate [RR = 0.72, 95% CI (0.58, 0.89), P = 0.003]. There were no significant differences between the two groups in the incidence of adverse events of infection [RR = 1.31, 95% CI (0.94, 1.81), P = 0.11] and pain [RR = 0.92, 95% CI (0.79, 1.08), P = 0.30]. Bone morphogenetic protein has certain advantages in treating open tibial fractures. It needs more high-quality articles to assess the long-term effect of different courses of treatments. The above conclusion still needs more high-quality randomized controlled trails to be verified owing to the limitations of the number and quality of systematic review included studies.


Ha X.,Peoples Hospital of Gansu Province
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals | Year: 2013

There are few reports of endothelial progenitor cells (EPCs) in peripheral blood have been found in patients with gastric cancer. We quantified EPCs in the peripheral blood of patients with gastric cancer, with the expectation that this approach might lead to a new marker for the diagnosis of gastric cancer. We enumerated CD34+/CD133+ EPCs in the peripheral blood of 145 subjects by use of flow cytometry. The quantity of peripheral blood EPCs in patients with gastric cancer are correlated with patient's age. In addition, the number of peripheral blood EPCs in patients with gastric cancer increased with tumor node metastasis stage and histological differentiation of the cancers, and with the operative status of the patients.


Wei X.,Peoples Hospital of Gansu Province
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2011

To analysis the prognostic factors of the sudden sensorineural hearing loss (SHL), to predict the prognosis of patient with SHL precisely. Two hundred and fifty-six cases (280 ears) with SHL were reviewed retrospectively during January 2005 to Jun 2009. Possible influence factor was analysis with logistic stepwise regression to investigate the correlation with prognosis. All verified influence factors was analysis with single-factor analysis of variance. The prognosis of SHL related to age, the trouble time before treatment, vertigo with(or) tinnitus, degree of hearing loss, the patterns of audiogram, but not to sex and trouble ear. With increasing of age, the prognosis of SHL becomes poor. Especially after 40 years old, the prognosis gets worse. Regarding to the relationship between the trouble time before treatment and the prognosis. The groups within 9 days have no significant difference (P > 0.05). There are significant difference between group of > 3-9 days and > 9-15 days (P < 0.05), > 9-15 days and > 15 days (P < 0.05). Compared with patients suffer from vertigo or (and) tinnitus, patients without vertigo or (and) tinnitus obtain a good result (P < 0.05). As for initial hearing threshold, flat (upgrade) subgroup, downgrade (upgrade) subgroup, upgrade subgroup and valley-mountain subgroup have no significant difference (P > 0.05). However, there are significant difference between upgrade subgroup and flat subgroup, upgrade subgroup and all the others. Furthermore, comparison with the low,midst, severe and profound hearing loss, the prognosis among them is no statistical different. But statistical different exists between media and profound subgroup. The factor of age, the trouble time before treatment, vertigo with (or) tinnitus, degree of hearing loss, the patterns of audiogram are correlated with prognosis of SHL. The prognosis has a poor tendency with age increasing, especially after 40 years old. Good result maybe achieved within 9 visiting days. After that, the recovery rates declined obviously. Patients have company complication, such as vertigo or (and) tinnitus have poor prognosis. The downgrade subgroup of audiogram has high incidence rate although upgrade subgroup has a good prognosis. The level of hearing loss before medication is not a simple linear correlation with prognosis. Media hearing loss demonstrate the best prognosis. On the contrary, profound hearing loss has the worst prognosis. With hearing threshold added, the total recovery rates goes down.


Dong F.,Peoples Hospital of Gansu Province | Ha X.-Q.,Peoples Hospital of Gansu Province
Chinese Medical Journal | Year: 2010

Objective To review the effect of endothelial progenitor cells in neovascularization as well as their application to the therapy of tumors. Data sources The data used in this review were mainly from PubMed for relevant English language articles published from 1997 to 2009. The search term was "endothelial progenitor cells". Study selection Articles regarding the role of endothelial progenitor cells in neovascularization and their application to the therapy of tumors were selected. Results Endothelial progenitor cells isolated from bone marrow, umbilical cord blood and peripheral blood can proliferate, mobilize and differentiate into mature endothelial cells. Experiments suggest endothelial progenitor cells take part in forming the tumor vascular through a variety of mechanisms related to vascular endothelial growth factor, matrix metalloproteinases, chemokine stromal cell-derived factor 1 and its receptor C-X-C receptor-4, erythropoietin, Notch signal pathway and so on. Evidence demonstrates that the number and function change of endothelial progenitor cells in peripheral blood can be used as a biomarker of the response of cancer patients to anti-tumor therapy and predict the prognosis and recurrence. In addition, irradiation temporarily increased endothelial cells number and decreased the endothelial progenitor cell counts in animal models. Meanwhile, in preclinical experiments, therapeutic gene-modified endothelial progenitor cells have been approved to attenuate tumor growth and offer a novel strategy for cell therapy and gene therapy of cancer. Conclusions Endothelial progenitor cells play a particular role in neovascularization and have attractively potential prognostic and therapeutic applications to malignant tumors. However, a series of problems, such as the definitive biomarkers of endothelial progenitor cells, their interrelationship with radiotherapy and their application in cell therapy and gene therapy of tumors, need further investigation.


Zhai L.,Peoples Hospital of Gansu Province | Liu Q.,Peoples Hospital of Gansu Province
Cancer Research and Clinic | Year: 2015

Objective To analyze the clinicopathologic features, diagnosis and different diagnosis of solid papillary carcinoma (SPC) of the breast. Methods The clinical data and pathological section, including HE and immunohistochemical staining slice, of 12 cases of SPC in People's Hospital of Shanxi Province from December 2008 to August 2014 were analyzed. Simultaneously, a literature search was conducted by utilizing Pub Med for all reported cases in English from November 1995 to October 2014. Results All cases were female, aged from 50 to 77 years old, manifested as mass or nipple discharge. Histologically, all cases appeared as multiple, well-circumscribed nodules filled by monotonous, low-moderate grade cell separated by fibrovascular cores. Immunohistochemically, CgA, Syn, ER and PR were positive in 12, 11, 9 and 5 cases, respectively. Her-2 presented as 1+ in 1 case (1/12) with invasive mucinous carcinoma, and locally poor positive in 2 cases without invasive component, while CK5/6 was negative in all cases. p63 and CK5/6 were positive in 6 and 5 cases at the location of axis of papillary, respectively. Meanwhile, 8 and 7 cases at the border of duct, respectively. Conclusion SPC is an uncommon breast tumor, which presents with specific clinicopathologic features.

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