Peoples Hospital of Binzhou City

Binzhou, China

Peoples Hospital of Binzhou City

Binzhou, China

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Zhai N.,Binzhou Medical University | Xia Y.,Binzhou Medical University | Yin R.,Peoples Hospital Of Binzhou City | Liu J.,Binzhou Medical University | Gao F.,Binzhou Medical University
OncoTargets and Therapy | Year: 2016

Non-small-cell lung cancer (NSCLC) is one of the leading causes of cancer-related death worldwide, and the 5-year survival rate is still low despite advances in diagnosis and therapeutics. A long noncoding RNA (lncRNA) HOX antisense intergenic RNA (HOTAIR) has been revealed to play important roles in NSCLC carcinogenesis but the detailed mechanisms are still unclear. In the current study, we aimed to investigate the regulation between the lncRNA HOTAIR and p53 in the NSCLC patient samples and cell lines. Our results showed that HOTAIR expression was significantly higher in the cancer tissues than that in the adjacent normal tissue, and was negatively correlated with p53 functionality rather than expression. When p53 was overexpressed in A549 cells, the lncRNA HOTAIR expression was downregulated, and the cell proliferation rate and cell invasion capacity decreased as a consequence. We identified two binding sites of p53 on the promoter region of HOTAIR, where the p53 protein would bind to and suppress the HOTAIR mRNA transcription. Inversely, overexpression of lncRNA HOTAIR inhibited the expression of p53 in A549 cells. Mechanistic studies revealed that HOTAIR modified the promoter of p53 and enhanced histone H3 lysine 27 trimethylation (H3K27me3). These studies identified a specific negative regulation loop of lncRNA HOTAIR and p53 in NSCLC cells, which revealed a new understanding of tumorigenesis in p53 dysfunction NSCLC cells. © 2016 Zhai et al.


Liu F.,Shandong University | Liu F.,Shandong Medical College | Li Q.,Shandong University | Zhang P.,Peoples Hospital of Binzhou City | And 2 more authors.
Molecular Medicine Reports | Year: 2015

Non-small cell lung cancer (NSCLC) remains the leading cause of cancer-related mortality despite the fact that great advances have been made in therapeutic treatment methods. Therefore, in the present study, the role of adenovirus-mediated retinoblastoma 94 (Ad-RB94) gene therapy in NSCLC was investigated. Following treatment with Ad-RB94, the proportion of A549 cells in the G2/M phase was increased. In the mouse xenograft model, the overexpression of RB94 inhibited the tumor growth compared with the control group and the Ad-LacZ-treated group. In the transplanted tumors, the overexpression of RB94 induced the apoptosis of tumors as well as an increase in the mRNA levels of cyclinB1. In conclusion, the results of the present study suggested that RB94 may effectively inhibit NSCLC tumor cell growth by inducing G2/M cell cycle arrest and apoptosis, indicating that RB94 may be a promising candidate for adjuvant therapy with radiation or chemotherapy in NSCLC.


Li J.,Peoples Hospital of Binzhou City | Diao H.,Binzhou Medical College | Zhao D.,Binzhou Medical College | Zhang J.,Cancer Institute of New Jersey
Archives of Medical Science | Year: 2010

Introduction: Chemokine ligand 5, also known as CCL5 or regulated on activation normal T-cell expressed and secreted (RANTES), is a chemokine expressed in inflamed tissue and capable of inducing migration of immature dendritic cells (DCs) or Langerhans cells. In this study, we explored the effect of RANTES on EG7 cells. Material and methods: In vivo, RANTES gene transfer reduced the tumourigenic capacity of EG7 and prolonged the survival of tumour-bearing mice. To reveal the underlying mechanism, we performed the following experiments and provided evidence to support our hypothesis of RANTES gene therapy for EG7. Higher natural killer (NK) cell and cytotoxic T lymphocyte (CTL) activity was induced after RANTES gene transfer, accompanied by higher levels of Th1 type cytokines (IL-2 and IFN-γ). Results: Tumour necrosis was also markedly observed in the tumour tissues after RANTES gene transfer, which was attributed to reduced expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP-2). Conclusions: We draw the conclusion that reduced tumourigenicity of EG7 after RANTES gene transfer can be attributed to higher NK cell and CTL activity, antiangiogenesis and higher levels of Th1 type cytokines induced by RANTES. These results support the notion that higher chemokine expression in tumour tissue elicits potent anti-tumour immunity. Copyright © 2010 Termedia & Banach.


Li K.,Shandong University | Li K.,Peoples Hospital of Binzhou City | Jiang S.,Shandong University
Sarcoidosis Vasculitis and Diffuse Lung Diseases | Year: 2014

Objectives:The aim of this study was to compare the diagnostic yield ofTBNA and EBUS-TBNA in the patients with suspected stage I and II sarcoidosis in the case of the same number of needle aspiration lymph nodes and the same lymph nodes needle aspiration times.Methods:A total of 62 patients with suspected stage I and II sarcoidosis were randomly divided into TBNA group and EBUS-TBNA group, based on the clinical and radiologic manifestations. Biopsy specimens of each patient in both groups were taken from two lymph nodes with two needle passes per lymph node.The diagnostic yields of subgroup were separately calculated including the 4th and 7th lymph nodes (referred to as group A), in other stations (group B), greater than 15 mm and less than 15 mm in the shortest diameter. Results: The diagnostic yield of TBNA and EBUS-TBNA for sarcoidosis was 64% and 93%, respectively (χ2=7.12, P<0.05). Subgroup analysis showed that the percentages of positive pathological diagnosis in group A for TBNA and EBUS-TBNA were 79% and 95% (χ2=3.47, P>0.05).The percentages of positive pathological diagnosis greater than 15 mm in the shortest diameter were 78% and 94% (χ2=2.29, P>0.05). Conclusion: The overall diagnostic yield of EBUS-TBNA for stage I and II sarcoidosis was higher than TBNA.However, Conventional TBNA has very high diagnostic yield similar to EBUS-TBNA, if the lymph nodes located on the 4th and 7th group or the shortest diameter was greater than 15 mm. © Mattioli 1885.


Li H.,Peoples Hospital of Binzhou City | Zhang F.H.,Peoples Hospital of Binzhou City | Wang Y.C.,Peoples Hospital of Binzhou City
Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2013

To verify the clinical efficacy of shu-stream point acupuncture combined with fire needle therapy in the treatment of hand osteoarthritis. Eighty cases were randomized into an acupuncture group (shu-stream point acupuncture combined with fire needle therapy) and a medication group(votalin emulgel). For the 42 cases in the acupuncture group, acupuncture at shu-stream points on the three yang meridians of the hand including Sanjian (LI 3), Zhongzhu (TE 3), Houxi (SI 3) and fire needle at Ashi points were applied, the treatments were given once every other day, 15 times as a treatment course. For the 38 cases in the medication group, votalin emulgel was prescribed for local embrocation, twice a day, 30 days as a treatment course. The visual analogue scale (VAS) of arthralgia, joint function score and the duration of morning stiffness, joint tenderness, swelling were observed before and after treatment in both groups. After two courses of clinical treatment, the VAS score of arthralgia, joint function score and the duration of morning stiffness, joint tenderness, swelling were statistically significant differences as compared with before treatment (all P < 0.01 ), the efficacy in the acupuncture group was superior to that in the medication group (all P < 0.01 ). The cured-markedly effective rate and total effective rate were 61.9% (26/42) and 95.2% (40/42) in the acupuncture group, and 36.8% (14/38) and 76.3% (29/38) in the medication group respectively, with statistically significant differences between the two groups (both P < 0.05). Shu-stream point acupuncture combined with fire needle therapy achieves a significant efficacy in the treatment of hand osteoarthritis.


Lu H.-E.,Peoples Hospital of Binzhou City | Chen Y.,Peoples Hospital of Binzhou City | Sun X.-B.,Peoples Hospital of Binzhou City | Tong B.,Peoples Hospital of Binzhou City | Fan X.-H.,Peoples Hospital of Binzhou City
RSC Advances | Year: 2015

Luteolin, a plant flavonoid, has a wide range of therapeutic e ffects. The aim of this study is to examine the effect of luteolin on diabetes-induced oxidative stress and inflammation in the retina of rats. A diabetic rat model was induced by intraperitoneal injection of streptozotocin (60 mg kg-1), and the rats were orally administrated luteolin in doses of 25, 50 and 100 mg kg-1 for 6 weeks. The results showed that luteolin administration prevented the diabetes-induced decrease in the antioxidant capacity and increases in malondialdehyde (MDA), 8-hydroxy-2′-deoxyguanosine (8-OHdG) and nitrotyrosine levels. Luteolin also inhibited the diabetes-induced elevation in the levels of IL-1β, VEGF and NF-κB. However, in the high dose group (100 mg kg-1), retinal glutathione (GSH) levels were restored to close to normal levels. These effects of luteolin were achieved without amelioration of the severity of hyperglycemia. These data suggest that luteolin can be effective for protection against diabetes-induced retinal neurodegeneration by inhibiting the levels of inflammatory markers and oxidative stress. © The Royal Society of Chemistry 2015.


Sun X.-B.,Peoples Hospital of Binzhou City | Lu H.-E.,Peoples Hospital of Binzhou City | Chen Y.,Peoples Hospital of Binzhou City | Fan X.-H.,Peoples Hospital of Binzhou City | Tong B.,Peoples Hospital of Binzhou City
Pharmazie | Year: 2014

Elevated intraocular pressure (IOP) is considered as the major risk factor for the loss of retinal ganglion cells (RGCs) and their axons in glaucoma. Lithium chloride (LiCl) inhibits glycogen synthase kinase-3 beta (GSK-3β) and attends PERK-induced endoplasmic reticulum stress (ERs) transition. PERK is a type I transmembrane protein located in the endoplasmic reticulum. PERK pathway activation takes place in ERs early inhibiting protein synthnesis to protect cell and promote cell survival. Here, we firstly evaluate that LiCl reduced IOP when administered intraperitoneally. After 6 weeks, IOP dropped by around 21.9% in LiCl treated rats. Then we investigated the effects of LiCl on PERK-mediated signaling pathways. LiCl treatment activated PERK and inhibited the expression of ROCK-1and ROCK-2 in a rat model of glaucoma. Collectively, these results suggest that LiCl reduced the IOP through the phosphorylation of PERK by the regulation of PERK/ROCK signaling in glaucoma rat model.


Liu Y.-L.,Shandong University | Liu Y.-L.,Affiliated Hospital of Binzhou Medical College | Liu P.-F.,Affiliated Hospital of Binzhou Medical College | Liu H.-E.,Peoples Hospital of Binzhou City | And 2 more authors.
Pathology Research and Practice | Year: 2014

The aim of this study was to investigate the associations between STAT5 gene polymorphisms and glioblastoma (GBM) risk predisposition. We undertook a case-control study to analyze two STAT5 polymorphisms (STAT5a rs11079041 and STAT5b rs2293157) in a Han Chinese population, by extraction of genomic DNA from the peripheral blood of 328 patients with glioma and 342 control participants, and performed STAT5 genotyping using DNA sequencing. The obtained results indicated that overall, no statistically significant association was observed in STAT5a rs11079041. Nevertheless, STAT5b rs2293157 G/T genotype was at increased risk of glioma (P=0.001). Furthermore, rs2293157T allele was more significantly prognostic in patients suffering from glioblastoma compared to other subtypes of gliomas (P<0.001; odds ratio (OR) = 5.14, CI 95%: 2.70-9.79). These findings led us to conclude that polymorphism in STAT5b rs2293157 G/T was observed to be associated with susceptibility of glioblastoma. Nevertheless, further investigation with a later confirmation in another ethnical or geographical cohort is required. © 2014 Elsevier GmbH.


PubMed | Peoples Hospital of Binzhou City
Type: Journal Article | Journal: Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2013

To verify the clinical efficacy of shu-stream point acupuncture combined with fire needle therapy in the treatment of hand osteoarthritis.Eighty cases were randomized into an acupuncture group (shu-stream point acupuncture combined with fire needle therapy) and a medication group(votalin emulgel). For the 42 cases in the acupuncture group, acupuncture at shu-stream points on the three yang meridians of the hand including Sanjian (LI 3), Zhongzhu (TE 3), Houxi (SI 3) and fire needle at Ashi points were applied, the treatments were given once every other day, 15 times as a treatment course. For the 38 cases in the medication group, votalin emulgel was prescribed for local embrocation, twice a day, 30 days as a treatment course. The visual analogue scale (VAS) of arthralgia, joint function score and the duration of morning stiffness, joint tenderness, swelling were observed before and after treatment in both groups.After two courses of clinical treatment, the VAS score of arthralgia, joint function score and the duration of morning stiffness, joint tenderness, swelling were statistically significant differences as compared with before treatment (all P < 0.01 ), the efficacy in the acupuncture group was superior to that in the medication group (all P < 0.01 ). The cured-markedly effective rate and total effective rate were 61.9% (26/42) and 95.2% (40/42) in the acupuncture group, and 36.8% (14/38) and 76.3% (29/38) in the medication group respectively, with statistically significant differences between the two groups (both P < 0.05).Shu-stream point acupuncture combined with fire needle therapy achieves a significant efficacy in the treatment of hand osteoarthritis.

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