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Li J.,Peoples Hospital of Binzhou City | Diao H.,Binzhou Medical College | Zhao D.,Binzhou Medical College | Zhang J.,Cancer Institute of New Jersey
Archives of Medical Science

Introduction: Chemokine ligand 5, also known as CCL5 or regulated on activation normal T-cell expressed and secreted (RANTES), is a chemokine expressed in inflamed tissue and capable of inducing migration of immature dendritic cells (DCs) or Langerhans cells. In this study, we explored the effect of RANTES on EG7 cells. Material and methods: In vivo, RANTES gene transfer reduced the tumourigenic capacity of EG7 and prolonged the survival of tumour-bearing mice. To reveal the underlying mechanism, we performed the following experiments and provided evidence to support our hypothesis of RANTES gene therapy for EG7. Higher natural killer (NK) cell and cytotoxic T lymphocyte (CTL) activity was induced after RANTES gene transfer, accompanied by higher levels of Th1 type cytokines (IL-2 and IFN-γ). Results: Tumour necrosis was also markedly observed in the tumour tissues after RANTES gene transfer, which was attributed to reduced expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase (MMP-2). Conclusions: We draw the conclusion that reduced tumourigenicity of EG7 after RANTES gene transfer can be attributed to higher NK cell and CTL activity, antiangiogenesis and higher levels of Th1 type cytokines induced by RANTES. These results support the notion that higher chemokine expression in tumour tissue elicits potent anti-tumour immunity. Copyright © 2010 Termedia & Banach. Source

Liu Y.-L.,Shandong University | Liu Y.-L.,Affiliated Hospital of Binzhou Medical College | Liu P.-F.,Affiliated Hospital of Binzhou Medical College | Liu H.-E.,Peoples Hospital of Binzhou City | And 2 more authors.
Pathology Research and Practice

The aim of this study was to investigate the associations between STAT5 gene polymorphisms and glioblastoma (GBM) risk predisposition. We undertook a case-control study to analyze two STAT5 polymorphisms (STAT5a rs11079041 and STAT5b rs2293157) in a Han Chinese population, by extraction of genomic DNA from the peripheral blood of 328 patients with glioma and 342 control participants, and performed STAT5 genotyping using DNA sequencing. The obtained results indicated that overall, no statistically significant association was observed in STAT5a rs11079041. Nevertheless, STAT5b rs2293157 G/T genotype was at increased risk of glioma (P=0.001). Furthermore, rs2293157T allele was more significantly prognostic in patients suffering from glioblastoma compared to other subtypes of gliomas (P<0.001; odds ratio (OR) = 5.14, CI 95%: 2.70-9.79). These findings led us to conclude that polymorphism in STAT5b rs2293157 G/T was observed to be associated with susceptibility of glioblastoma. Nevertheless, further investigation with a later confirmation in another ethnical or geographical cohort is required. © 2014 Elsevier GmbH. Source

Liu F.,Shandong University | Liu F.,Shandong Medical College | Li Q.,Shandong University | Zhang P.,Peoples Hospital of Binzhou City | And 2 more authors.
Molecular Medicine Reports

Non-small cell lung cancer (NSCLC) remains the leading cause of cancer-related mortality despite the fact that great advances have been made in therapeutic treatment methods. Therefore, in the present study, the role of adenovirus-mediated retinoblastoma 94 (Ad-RB94) gene therapy in NSCLC was investigated. Following treatment with Ad-RB94, the proportion of A549 cells in the G2/M phase was increased. In the mouse xenograft model, the overexpression of RB94 inhibited the tumor growth compared with the control group and the Ad-LacZ-treated group. In the transplanted tumors, the overexpression of RB94 induced the apoptosis of tumors as well as an increase in the mRNA levels of cyclinB1. In conclusion, the results of the present study suggested that RB94 may effectively inhibit NSCLC tumor cell growth by inducing G2/M cell cycle arrest and apoptosis, indicating that RB94 may be a promising candidate for adjuvant therapy with radiation or chemotherapy in NSCLC. Source

Li H.,Peoples Hospital of Binzhou City | Zhang F.H.,Peoples Hospital of Binzhou City | Wang Y.C.,Peoples Hospital of Binzhou City
Zhongguo zhen jiu = Chinese acupuncture & moxibustion

To verify the clinical efficacy of shu-stream point acupuncture combined with fire needle therapy in the treatment of hand osteoarthritis. Eighty cases were randomized into an acupuncture group (shu-stream point acupuncture combined with fire needle therapy) and a medication group(votalin emulgel). For the 42 cases in the acupuncture group, acupuncture at shu-stream points on the three yang meridians of the hand including Sanjian (LI 3), Zhongzhu (TE 3), Houxi (SI 3) and fire needle at Ashi points were applied, the treatments were given once every other day, 15 times as a treatment course. For the 38 cases in the medication group, votalin emulgel was prescribed for local embrocation, twice a day, 30 days as a treatment course. The visual analogue scale (VAS) of arthralgia, joint function score and the duration of morning stiffness, joint tenderness, swelling were observed before and after treatment in both groups. After two courses of clinical treatment, the VAS score of arthralgia, joint function score and the duration of morning stiffness, joint tenderness, swelling were statistically significant differences as compared with before treatment (all P < 0.01 ), the efficacy in the acupuncture group was superior to that in the medication group (all P < 0.01 ). The cured-markedly effective rate and total effective rate were 61.9% (26/42) and 95.2% (40/42) in the acupuncture group, and 36.8% (14/38) and 76.3% (29/38) in the medication group respectively, with statistically significant differences between the two groups (both P < 0.05). Shu-stream point acupuncture combined with fire needle therapy achieves a significant efficacy in the treatment of hand osteoarthritis. Source

Sun X.-B.,Peoples Hospital of Binzhou City | Lu H.-E.,Peoples Hospital of Binzhou City | Chen Y.,Peoples Hospital of Binzhou City | Fan X.-H.,Peoples Hospital of Binzhou City | Tong B.,Peoples Hospital of Binzhou City

Elevated intraocular pressure (IOP) is considered as the major risk factor for the loss of retinal ganglion cells (RGCs) and their axons in glaucoma. Lithium chloride (LiCl) inhibits glycogen synthase kinase-3 beta (GSK-3β) and attends PERK-induced endoplasmic reticulum stress (ERs) transition. PERK is a type I transmembrane protein located in the endoplasmic reticulum. PERK pathway activation takes place in ERs early inhibiting protein synthnesis to protect cell and promote cell survival. Here, we firstly evaluate that LiCl reduced IOP when administered intraperitoneally. After 6 weeks, IOP dropped by around 21.9% in LiCl treated rats. Then we investigated the effects of LiCl on PERK-mediated signaling pathways. LiCl treatment activated PERK and inhibited the expression of ROCK-1and ROCK-2 in a rat model of glaucoma. Collectively, these results suggest that LiCl reduced the IOP through the phosphorylation of PERK by the regulation of PERK/ROCK signaling in glaucoma rat model. Source

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