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Yuan J.,People Hospital of Cangzhou city | Lan T.,People Hospital of Cangzhou city | Liu J.,People Hospital of Cangzhou city | Wang G.,People Hospital of Cangzhou city | And 5 more authors.
Biomedical Research (India) | Year: 2015

This study was conducted to enrich and purify the active constituent in Polygonum cuspidatum, and to study its anticancer activity. Extraction conditions of Polygonum cuspidatum were optimized by investigating the macroporous resin model, flow rate and ethanol concentration. MTT assay and electron microscopy were used to analyze the inhibitory effect of Polygonum cuspidatum extract on human SW480 cells. During the investigation of enrichment and purification process, the optimal conditions for enrichment of Polygonum cuspidatum extract with D101 macroporous resin were identified to be a 3-fold amount of 75% ethanol, and a flow rate of 2 BV/h. Under electron microscope, nuclear morphological changes are seen in SW480 cells treated with Polygonum cuspidatum extract; apoptotic characteristics are significant, and apoptotic bodies are formed eventually. MTT assay results showed that the inhibitory effect of Polygonum cuspidatum extract on SW480 cells increases with the increase of concentration and prolongation of action time within the experimental concentration range, which proves a dose- and time-dependence of action. Polygonum cuspidatum extract has a marked inhibitory effect on human colon cancer SW480 cells. © 2015 Scientific Publishers of India. All rights reserved.

Yuan J.-L.,People Hospital of Cangzhou City | Wang S.-M.,People Hospital of Cangzhou City | Lan T.,People Hospital of Cangzhou City | Liu J.-Z.,People Hospital of Cangzhou City | And 3 more authors.
Tropical Journal of Pharmaceutical Research | Year: 2015

Purpose: To explore the anti-hepatoma effect of Gan-Ai-Xiao Decoction (GAXD), a folk remedy. Methods: High performance liquid chromatography (HPLC) was used to identify the major chemical components of GAXD ethanol extract (EE). The cytotoxic effect of GAXD EE against HepG2 cells was measured by methyl thiazolyl tetrazolium (MTT) assay. Flow cytometry and Western blot were used to study the effect of GAXD EE on apoptosis and apoptotic proteins (Bcl-2, Bax and caspase-3) in HepG2 cells. Xenograft assay was used to evaluate the anti-hepatoma effect of GAXD EE in vivo. Results: Four components were identified in GAXD EE by HPLC. The results of MTT and flow cytometry assays indicated that GAXD EE significantly reduced HepG2 cells viability (p < 0.05) and induced its apoptosis. The results of Western blot assay suggested that GAXD EE down-regulated the expression of anti-apoptotic protein (Bcl-2) and up-regulated the expression of pro-apoptotic proteins (Bax and caspase-3) in HepG2 cells. Furthermore, the results of xenograft assay showed that GAXD EE significantly inhibited the growth of HepG2 cells-induced tumor (p < 0.05) without any effect on the body weight of nude mice. Conclusion:  GAXD has anti-hepatoma activity, and the mechanism is associated with apoptosis. © Pharmacotherapy Group.

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