Peninsular and Marine Fish Genetic Resources Center

Cochin, India

Peninsular and Marine Fish Genetic Resources Center

Cochin, India
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Sood N.,ICAR National Bureau of Fish Genetic Resources | Swaminathan T.R.,Peninsular and Marine Fish Genetic Resources Center | Yadav M.K.,ICAR National Bureau of Fish Genetic Resources | Pradhan P.K.,ICAR National Bureau of Fish Genetic Resources | And 2 more authors.
Diseases of Aquatic Organisms | Year: 2017

Goldfish Carassius auratus is the most popular ornamental species, widely present in private and public aquaria. In the present case, 2 goldfish exhibited bilateral, multiple, variably sized, round, pale-white, soft, protruding masses on the body. The microscopic examination of the masses revealed well-differentiated adipocytes infiltrating the subcutaneous skeletal muscle bundles. The histological lesions were consistent with infiltrative lipoma. To our knowledge, this is the first report of cutaneous infiltrative lipoma in goldfish. © Inter-Research 2017.


Ho H.-C.,National Museum of Marine Biology and Aquarium | Ho H.-C.,National Dong Hwa University | Meleppura R.K.,Center for Marine Living Resources and Ecology | Bineesh K.K.,Peninsular and Marine Fish Genetic Resources Center
Zootaxa | Year: 2016

A new species of Chaunax is described on the basis of eight type and five non-type specimens. This species belongs to the Chaunax abei species group and can be distinguished from congeners in the group by having a continuous tooth patch on the vomer, not divided into two patches, and four or five neuromasts in the lower preopercular series. It can be further separated by the following combination of characters: large green spots on dorsal surface; simple spinules on dorsal sur-face; 12 pectoral-fin rays; 13-16 neuromasts in pectoral series; 30-37 neuromasts in lateral-line proper; typically four neu-romasts on caudal-fin base; typically 7 neuromasts in mandible; typically 12 gill rakers on second gill arch; gill chamber and buccal cavity pale; and peritoneum black. © Copyright 2016 Magnolia Press.


Kumar R.,Peninsular and Marine Fish Genetic Resources Center | Gopalakrishnan A.,Central Marine Fisheries Research Institute | Divya P.R.,Peninsular and Marine Fish Genetic Resources Center | Basheer V.S.,Peninsular and Marine Fish Genetic Resources Center | And 4 more authors.
Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis | Year: 2017

Macrobrachium rosenbergii, giant freshwater prawn, is one of the most commercially important crustaceans. In the present study, primers for ATPase 6/8 region of mt-DNA were designed and successfully amplified (827 bp) in the species. The nucleotide variation in ATPase 6/8 gene revealed the population structuring in natural populations of M. rosenbergii in Indian waters. A total of 35 haplotypes were observed in 93 individuals collected from different locations. Low nucleotide diversity and high haplotype diversity were noticed for the ATPase 6/8 gene. Significant pairwise FST and, haplotype network indicated occurrence of distinct populations. Observed mismatch distribution and Tajima’s D test suggested demographical stability of giant freshwater prawn. The genetic stock structure revealed in this study will be helpful for conservation and management of stocks of M. rosenbergii in Indian waters. © 2016 Informa UK Limited, trading as Taylor & Francis Group.


Vineesh N.,Peninsular and Marine Fish Genetic Resources Center | Divya P.,Peninsular and Marine Fish Genetic Resources Center | Kathirvelpandian A.,Peninsular and Marine Fish Genetic Resources Center | Mohitha C.,Peninsular and Marine Fish Genetic Resources Center | And 3 more authors.
Marine Biodiversity | Year: 2017

Population genetic structure of the pelagic narrow-barred Spanish mackerel (Scomberomorus commerson) was examined in 10 populations from the Indo-West Pacific region, based on mitochondrial control region DNA. We examined genetic composition of 56 individuals of S. commerson from the Arabian Sea, Bay of Bengal, and compared them with 40 individuals from previously published data sampled from the ROPME Seas area and South West Pacific Ocean. The overall nucleotide and haplotype diversities were estimated to be 0.072 and 0.995, respectively. Demographic parameters such as Tajima’s D and Fu’s Fs statistics were negative, but non-significant across all the populations, except for New Caledonia in the South West Pacific, indicating a stable population of S. commerson in this region. To assess intra-specific patterns of connectivity, we constructed statistical parsimony networks among haplotypes and estimated time of most recent population expansion. AMOVA and FST values among Indian and Arabian regions revealed non-significant variation among the populations, while separation was noted among the samples of the northern and eastern Indian oceans, as well as with South West Pacific. A parsimony network analysis provided evidence for four separate population segment/stocks in the Indian Ocean and Pacific Ocean, raising a conservation concern. Our results support a need for regional cooperation to ensure the sustainable exploitation of S. commerson in the Indo-West Pacific region. © 2017 Senckenberg Gesellschaft für Naturforschung and Springer-Verlag Berlin Heidelberg


PubMed | Central Marine Fisheries Research Institute and Peninsular and Marine Fish Genetic Resources Center
Type: | Journal: Molecular phylogenetics and evolution | Year: 2017

Pomfrets (Genus Pampus) are commercially important fishes in the Indo Pacific region. The systematics of this genus is complicated due to morphological similarities between species. The silver pomfret from Indian waters has long been considered to be Pampus argenteus. The objective of the study was to utilize the mitochondrial COI gene to establish the molecular identity of the silver pomfret distributed in Indian waters and to resolve the phylogenetic relationships among Pampus species in the world based on sequence data in the NCBI database. Seven valid Pampus species are identified in this study. The mean genetic divergence value calculated between clades representing these species was 7.9%. The mean genetic distance between the so-called Pampus argenteus from Indian waters and sequences attributed to P. argenteus from the South China Sea, where the neotype of this species was collected, was found to be greater than 12%, strongly supporting the likelihood of the Indian species being distinct. The Indian Pampus species show very close affinity to P. cinereus, with inter species differences less than 2%. The taxonomic identity of the silver pomfret in India is also discussed here, in light of molecular and morphological evidence.


Bain P.A.,CSIRO | Basheer V.S.,Peninsular and Marine Fish Genetic Resources Center | Gregg A.,CSIRO | Jena J.K.,National Bureau of Fish Genetic Resources | Kumar A.,CSIRO
Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology | Year: 2016

Fluoxetine (FLX) is one of numerous pharmaceuticals found in treated municipal wastewater discharged to the environment. In the present study, we investigated the effects of short-term (96 h) waterborne FLX exposure (1 μg/L or 100 μg/L) on the expression of selected genes in brain, liver, and gonads of female Murray-Darling rainbowfish (Melanotaenia fluviatilis), a small-bodied teleost of ecotoxicological relevance in the Australasia region. Plasma 17β-estradiol (E2) levels were also determined. In the brain, no significant changes in mRNA levels were observed for the selected genes. In ovaries, 100 μg/L FLX caused a 10-fold downregulation of aromatase A (cyp19a1a) mRNA and a 4-fold upregulation of estrogen receptor α (esr1) mRNA levels. In liver, mRNA levels for vitellogenin A (vtga) and choriogenin L (chgl) were downregulated by 50-fold and 18-fold compared with controls, respectively, in response to 100 μg/L FLX. Concentrations of E2 in plasma were significantly lower than controls in response to 100 μg/L FLX. This could be attributable to a decrease in estrogen biosynthesis as a result of the observed downregulation of cyp19a1a mRNA. To establish whether the observed changes in gene expression could be explained by the modulation of selected nuclear receptors by FLX, we employed panel of reporter gene assays in agonistic and antagonistic modes. Apart from minor activation of ERα after exposure to high concentrations (5 μM), FLX did not activate or inhibit the nuclear receptors tested. Further study is required to determine whether the observed downregulation of ovarian aromatase expression and liver estrogen-regulated genes also occurs at environmentally relevant FLX concentrations over longer exposure periods. © 2016 Published by Elsevier Inc.


PubMed | Peninsular and Marine Fish Genetic Resources Center
Type: Journal Article | Journal: In vitro cellular & developmental biology. Animal | Year: 2015

Cyprinus carpio koi fin (CCKF) cell line was established and characterized from the caudal fin tissue of ornamental common carp, C. carpio koi. This cell line has been maintained in L-15 medium supplemented with 15% foetal bovine serum (FBS) and subcultured more than 52 times over a period of 24 mo. The CCKF cell line consisted of epithelial cells and was able to grow at temperatures between 22 and 35C with an optimum temperature of 28C. The growth rate of these cells increased as the proportion of FBS increased from 2 to 20% with optimum growth at the concentrations of 15% FBS. Karyotype analysis revealed that the modal chromosome number of CCKF cells was 2n=100. Partial amplification and sequencing of fragments of two mitochondrial genes 16S rRNA and COI confirmed that CCKF cell line originated from ornamental common carp. The CCKF cells showed strong reaction to the cytokeratin marker, indicating that it was epithelial in nature. The extracellular products of Vibrio cholerae MTCC 3904 and Aeromonas hydrophila were toxic to the CCKF cells and not susceptible to viral nervous necrosis virus (VNNV). These CCKF cells were confirmed for the absence of Mycoplasma sp. by polymerase chain reaction. Furthermore, 90% of viable cells could be effectively revived 4 mo after cryopreservation from CCKF cell population suggesting the possibility of long-term storage of the cells.


PubMed | National Bureau of Fish Genetic Resources, Peninsular and Marine fish Genetic Resources Center and CSIRO
Type: | Journal: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP | Year: 2016

Fluoxetine (FLX) is one of numerous pharmaceuticals found in treated municipal wastewater discharged to the environment. In the present study, we investigated the effects of short-term (96h) waterborne FLX exposure (1g/L or 100g/L) on the expression of selected genes in brain, liver, and gonads of female Murray-Darling rainbowfish (Melanotaenia fluviatilis), a small-bodied teleost of ecotoxicological relevance in the Australasia region. Plasma 17-estradiol (E2) levels were also determined. In the brain, no significant changes in mRNA levels were observed for the selected genes. In ovaries, 100g/L FLX caused a 10-fold downregulation of aromatase A (cyp19a1a) mRNA and a 4-fold upregulation of estrogen receptor (esr1) mRNA levels. In liver, mRNA levels for vitellogenin A (vtga) and choriogenin L (chgl) were downregulated by 50-fold and 18-fold compared with controls, respectively, in response to 100g/L FLX. Concentrations of E2 in plasma were significantly lower than controls in response to 100g/L FLX. This could be attributable to a decrease in estrogen biosynthesis as a result of the observed downregulation of cyp19a1a mRNA. To establish whether the observed changes in gene expression could be explained by the modulation of selected nuclear receptors by FLX, we employed panel of reporter gene assays in agonistic and antagonistic modes. Apart from minor activation of ER after exposure to high concentrations (5M), FLX did not activate or inhibit the nuclear receptors tested. Further study is required to determine whether the observed downregulation of ovarian aromatase expression and liver estrogen-regulated genes also occurs at environmentally relevant FLX concentrations over longer exposure periods.


Swaminathan T.R.,Peninsular and Marine Fish Genetic Resources Center | Basheer V.S.,Peninsular and Marine Fish Genetic Resources Center | Kumar R.,Peninsular and Marine Fish Genetic Resources Center | Kathirvelpandian A.,Peninsular and Marine Fish Genetic Resources Center | And 2 more authors.
In Vitro Cellular and Developmental Biology - Animal | Year: 2015

Cyprinus carpio koi fin (CCKF) cell line was established and characterized from the caudal fin tissue of ornamental common carp, C. carpio koi. This cell line has been maintained in L-15 medium supplemented with 15% foetal bovine serum (FBS) and subcultured more than 52 times over a period of 24 mo. The CCKF cell line consisted of epithelial cells and was able to grow at temperatures between 22 and 35°C with an optimum temperature of 28°C. The growth rate of these cells increased as the proportion of FBS increased from 2 to 20% with optimum growth at the concentrations of 15% FBS. Karyotype analysis revealed that the modal chromosome number of CCKF cells was 2n = 100. Partial amplification and sequencing of fragments of two mitochondrial genes 16S rRNA and COI confirmed that CCKF cell line originated from ornamental common carp. The CCKF cells showed strong reaction to the cytokeratin marker, indicating that it was epithelial in nature. The extracellular products of Vibrio cholerae MTCC 3904 and Aeromonas hydrophila were toxic to the CCKF cells and not susceptible to viral nervous necrosis virus (VNNV). These CCKF cells were confirmed for the absence of Mycoplasma sp. by polymerase chain reaction. Furthermore, 90% of viable cells could be effectively revived 4 mo after cryopreservation from CCKF cell population suggesting the possibility of long-term storage of the cells. © 2015, The Society for In Vitro Biology.


PubMed | Peninsular and Marine Fish Genetic Resources Center and University of Lucknow
Type: Journal Article | Journal: In vitro cellular & developmental biology. Animal | Year: 2015

The establishment and characterization of a continuous cell line from the thymus of air-breathing fish Channa striatus are described. The cell line, designated C. striatus thymus (CST), has been subcultured over 71 times and shows optimal growth at 28C in Leibovitzs-15 (L-15) medium supplemented with 20% fetal bovine serum. The CST cells exhibited low plating efficiency which improved with increase in seeding density. The karyotype analysis revealed that CST cells have a normal diploid karyotype with 2n=40. Partial amplification and sequencing of two mitochondrial genes, viz. 16S ribosomal RNA (rRNA) and cytochrome oxidase I, confirmed that the cell line originated from C. striatus. CST cells were successfully transfected indicating their potential application for expression of recombinant proteins. In immunocytochemical staining, CST cells showed characteristics of epithelial cells. These cells were sensitive to extracellular products of Vibrio cholerae MTCC 3904 as well as to heavy metal mercuric chloride. The CST cell line would be a useful tool in functional genomic studies such as RNA interference and gene knockout as well as for cytotoxicity studies.

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