Entity

Time filter

Source Type


Lu L.,Peking University | Lu L.,Peking Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology | Nan J.,Peking University | Mi W.,Peking University | And 6 more authors.
FEBS Letters | Year: 2010

Microtubules are composed of polymerized α/β-tubulin heterodimers. Biogenesis of assembly-competent tubulin dimers is a complex multistep process that requires sequential actions of distinct molecular chaperones and cofactors. Tubulin folding cofactor A (TFCA), which captures β-tubulin during the folding pathway, has been identified in many organisms. Here, we report the crystal structure of Arabidopsis thaliana TFC A (KIESEL, KIS), which forms a monomeric three-helix bundle. The functional binding analysis demonstrated that KIS interacts with β-tubulin in plant. Furthermore, mutagenesis studies indicated that the α-helical regions of KIS participate in β-tubulin binding. Unlike the budding yeast TFC A, the two loop regions of KIS are not required for this interaction suggesting a distinct binding mechanism of TFC A to β-tubulin in plants. © 2010 Federation of European Biochemical Societies. Source


Lu L.,Peking University | Lu L.,Peking Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology | Nan J.,Peking University | Mi W.,Peking University | And 5 more authors.
Acta Crystallographica Section F: Structural Biology and Crystallization Communications | Year: 2010

Tubulin-folding cofactor A (TFC A) is a molecular post-chaperonin that is involved in the Β-tubulin-folding pathway. It has been identified in many organisms including yeasts, humans and plants. In this work, Arabidopsis thaliana TFC A was expressed in Escherichia coli and purified to homogeneity. After thrombin cleavage, a well diffracting crystal was obtained by the sitting-drop vapour-diffusion method at 289 K. The crystal diffracted to 1.6 Å resolution using synchrotron radiation and belonged to space group I41, with unit-cell parameters a = 55.0, b = 55.0, c = 67.4 Å. © 2010 International Union of Crystallography All rights reserved. Source

Discover hidden collaborations