Jin Z.,Shenzhen University |
Jin Z.,Shenzhen Key Laboratory of Micromolecule Innovative Drugs |
Jin Z.,Peking University Shenzhen |
Zhao Z.,Shenzhen University |
And 8 more authors.
Cancer | Year: 2013
BACKGROUND Endoglin (ENG) is a 180-kilodalton transmembrane glycoprotein that functions as a component of the transforming growth factor-β receptor complex. Recently, ENG promoter hypermethylation was reported in several human cancers. METHODS The authors examined ENG promoter hypermethylation using real-time, quantitative, methylation-specific polymerase chain reaction in 260 human esophageal tissues. RESULTS ENG hypermethylation demonstrated highly discriminative receiver operating characteristic curve profiles, clearly distinguishing esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EAC) from normal esophagus (P <.01). It is interesting to note that ENG normalized methylation values were significantly higher in ESCC compared with normal tissue (P <.01) or EAC (P <.01). The ENG hypermethylation frequency was 46.2% in ESCC and 11.9% in normal esophageal tissue, but increased early and sequentially during EAC-associated neoplastic progression to 13.3% in Barrett metaplasia (BE), 25% in dysplastic BE, and 26.9% in frank EAC. ENG hypermethylation was significantly higher in normal esophageal tissue from patients with ESCC (mean, 0.0186) than in normal tissue from patients with EAC (mean, 0.0117; P <.05). Treatment of KYSE220 ESCC cells with the demethylating agent 5-aza-2′-deoxycytidine was found to reverse ENG methylation and reactivate ENG mRNA expression. CONCLUSIONS Promoter hypermethylation of ENG appears to be a frequent, tissue-specific event in human ESCC and exhibits a field defect with promising biomarker potential for the early detection of ESCC. In addition, ENG hypermethylation occurs in a subset of human EAC, and early during BE-associated esophageal neoplastic progression. Cancer 2013;119:3604-3609. © 2013 American Cancer Society.
Snyder K.M.,Oregon State University |
Sikorska J.,Oregon State University |
Ye T.,Peking University Shenzhen |
Fang L.,CAS Shenzhen Institutes of Advanced Technology |
And 4 more authors.
Organic and Biomolecular Chemistry | Year: 2016
The effectiveness of computational tools in determining relative configurations of complex molecules is investigated, using natural products mandelalides A-D and coibamide A, towards a generalized recipe for the scientific community at large. Ultimately, continuing efforts in this vein will accelerate and strengthen relative structure elucidation of complex molecules, such as natural products. Molecular mechanics conformational search, quantum mechanical NMR chemical shift predictions, and DP4 analyses led to confirmation of the revised structures of mandelalides A-D and coibamide A. All chiral centers in the northern hemisphere of mandelalides A-D are inverted with respect to the originally proposed structures, in agreement with recent total syntheses of mandelalide A by Ye, Fürstner & Carter. In the case of coibamide A, it was found that Fang & Su's revision, in which both the macrocycle [MeAla11] and the side chain [HIV2] residues are inverted from l to d, was consistent with the authentic natural product and computations. © The Royal Society of Chemistry 2016.
Duan L.,Shenzhen Key Laboratory of Tissue Engineering |
Liang Y.,Peking University Shenzhen |
Ma B.,University of Zurich |
Zhu W.,Shenzhen Key Laboratory of Tissue Engineering |
Wang D.,Shenzhen Key Laboratory of Tissue Engineering
American Journal of Translational Research | Year: 2015
Loss of hyaline chondrocyte phenotype during the monolayer culture in vitro is a major obstacle for cellbased articular cartilage repair. Increasing evidence implicates an important role of the epigenetic regulation in maintaining the chondrocyte phenotype. DNA methylation, histone modifications and microRNAs have all been shown to contribute to chondrocyte dedifferentiation and hypertrophy. Moreover, the interplay among epigenetic regulators forms a complicated epigenetic network in regulating chondrocyte dedifferentiation. This review provides a detailed overview of the epigenetic regulation in maintaining the chondrocyte phenotype for chondrocyte-based cartilage repair. © 2015, E-Century Publishing Corporation. All rights Reserved.
Liu H.,Peking University Shenzhen |
Yang H.,Peking University Shenzhen |
Zhu D.,Peking University Shenzhen |
Sui X.,Peking University Shenzhen |
And 19 more authors.
Cell Research | Year: 2014
The applications of human pluripotent stem cell (hPSC)-derived cells in regenerative medicine has encountered a long-standing challenge: how can we efficiently obtain mature cell types from hPSCs? Attempts to address this problem are hindered by the complexity of controlling cell fate commitment and the lack of sufficient developmental knowledge for guiding hPSC differentiation. Here, we developed a systematic strategy to study hPSC differentiation by labeling sequential developmental genes to encompass the major developmental stages, using the directed differentiation of pancreatic β cells from hPSCs as a model. We therefore generated a large panel of pancreas-specific mono-and dual-reporter cell lines. With this unique platform, we visualized the kinetics of the entire differentiation process in real time for the first time by monitoring the expression dynamics of the reporter genes, identified desired cell populations at each differentiation stage and demonstrated the ability to isolate these cell populations for further characterization. We further revealed the expression profiles of isolated NGN3-eGFP + cells by RNA sequencing and identified sushi domain-containing 2 (SUSD2) as a novel surface protein that enriches for pancreatic endocrine progenitors and early endocrine cells both in human embryonic stem cells (hESC)-derived pancreatic cells and in the developing human pancreas. Moreover, we captured a series of cell fate transition events in real time, identified multiple cell subpopulations and unveiled their distinct gene expression profiles, among heterogeneous progenitors for the first time using our dual reporter hESC lines. The exploration of this platform and our new findings will pave the way to obtain mature β cells in vitro. © 2014 IBCB, SIBS, CAS All rights reserved.
Li C.,Lanzhou University of Technology |
Wang Y.,Peking University Shenzhen |
Tan Y.,Peking University Shenzhen |
Tan Y.,Lanzhou University of Technology |
And 3 more authors.
Chinese Journal of Organic Chemistry | Year: 2010
Stereospecific synthesis of (2S,4S)/(2S,4R)-4-fluoroglutamic acids base on natural chiral source (4R)-hydroxyproline was studied by using of Mitsunobo reaction, fluorination with perfluoro-1-butanesulfonyl fluoride and NaIO 4/RuO2 oxidation.