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Biswas S.,Jadavpur University | Chakraborty S.,Jadavpur University | Das S.,Peerless Hospital And y Research Center
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2012

This review aims at compiling the different mutational changes undergone by the factor VIII gene leading to hemophilia A. It has been difficult to characterize completely a genetic disorder like hemophilia A where the gene of concerns is large, its tissue-specific expression, and all affected individuals have different mutational patterns. In this review, point mutational, insertional and deletional patterns are sited. The data are collected from all round the world, although "Western" sources are predominate. Source


Biswas A.,ICMR Virus Unit | Sarkar K.,ICMR Virus Unit | Firdaus R.,ICMR Virus Unit | Saha K.,ICMR Virus Unit | And 6 more authors.
Asian Journal of Pharmaceutical and Clinical Research | Year: 2016

Objective: The objective was to study the serological prevalence of post-transfusion transmitted infections such as hepatitis C virus (HCV), hepatitis B virus (HBV), and HIV among multi-transfused thalassemic individuals of the Eastern India and the socio and financial difficulties faced by them. Methods: The study was carried out from January 2012 until December 2014 involving 1711 thalassemic major individuals. Blood serum was collected from each patient to perform ELISA for the detection of HBV and HCV seroprevalence. HIV seropositivity along with their hematological and liver function parameters were obtained from the transfusion centers and the host institutions. Other socio-economic conditions were obtained by predesigned proforma of the questionnaire. Results: 67.9% males and 32.1% females were present in our study population of which 75% were from rural area. The mean hemoglobin was found to be lower, whereas mean ferritin, bilirubin, and liver enzymes were much higher than the normal range. Only a handful of 19.76% of the fathers of thalassemic individuals had secondary education. 263 families (15.37%) were familiar with the chances of transfusion-transmitted infections (TTIs). The dominant TTI found within the population was HCV with 18.70% prevalence followed by HIV (3.74%) and HBV (3.33%). 82.93% of the affected families suffered poverty with a meager monthly income within Rs. 5000 fighting against high costs of transfusion and related treatments. Conclusion: Our study reflects the different socio-economic and psychological burdens faced by the thalassemia patients and their families. The high rate of TTIs highlights the need for stringent screening of blood or blood products before administration. © 2016, Asian Journal of Pharmaceutical and Clinical Research. All rights reserved. Source


Chakraborty S.,Peerless Hospital And y Research Center | Kallner A.,Karolinska University Hospital
Clinica Chimica Acta | Year: 2015

Introduction: Spurious hyperphosphatemia is a relatively common phenomenon in patients with monoclonal gammopathy which can compromise patient safety. A cost-effective routine method is desirable for laboratories to reduce or eliminate the protein interference. Methods: A protein free sample was obtained after precipitation with PEG-6000 (polyethylene glycol). S-phosphate concentration was measured with routine wet chemistry method in normal and monoclonal gammopathy samples before and after precipitation. Monoclonal samples were also measured by dry chemistry that includes an ultrafiltration step. The protein pattern before and after precipitation was checked by electrophoresis. Results: No effect of PEG precipitation on S-phosphate concentration was demonstrated in normal serum. After PEG precipitation of proteins in monoclonal gammopathies serum wet chemistry gave the same results as dry chemistry i.e. after ultrafiltration. Conclusion: PEG precipitation of proteins offers a cost-effective method to eliminate protein interferences in monoclonal gammopathies. © 2014 Elsevier B.V. Source


Roy M.,Jadavpur University | Das R.,Jadavpur University | Kundu A.,Jadavpur University | Karmakar S.,Jadavpur University | And 4 more authors.
Agriculture | Year: 2015

Environmental and health safety of recycled slaughterhouse wastes-derived fertilizer and the produce obtained through its application is not well understood. Waste bovine blood and rumen digesta were mixed, cooked and sun-dried to obtain bovine-blood-and-rumen-digesta-mixture (BBRDM, NPK 30.36:1:5.75). 1.26 ± 0.18 log CFU mL-1 fecal coliforms were recovered in BBRDM. E. coli O157:H7, Mycobacteria, Clostridium sp., Salmonella sp., Bacillus sp. and Brucella sp. were absent. No re-growth of pathogens was observed after 60 days storage in sealed bags and in the open. However, prions and viruses were not evaluated. Heavy metals (Pb, Cr, Cd, Cu, Zn, As, Ni, Mn) concentrations in BBRDM were within internationally permissible limits. BBRDM was applied for field cultivation of tomato during 2012-2013 and 2013-2014. Lycopene and nitrate contents of BBRDM-grown tomatoes were higher than Diammonium phosphate (DAP) + potash-grown tomatoes because BBRDM supplied 2.5 times more the amount of nitrogen than DAP (NPK 18:46:0) + potash (NPK 0:0:44). Heavy metals and nitrate/nitrite concentrations in tomatoes were within internationally acceptable limits. BBRDM-grown tomatoes showed no mutagenic activity in the Ames test. Sub-acute toxicity tests on Wistar rats fed with BBRDM-grown tomatoes did not show adverse clinical picture. Thus, no immediate environmental or health risks associated with BBRDM and the tomatoes produced were identified. Source


Chakraborty S.,Jadavpur University | Biswas S.,Jadavpur University | Sa B.,Jadavpur University | Das S.,Peerless Hospital And y Research Center | Dey R.,Jadavpur University
Colloids and Surfaces A: Physicochemical and Engineering Aspects | Year: 2014

Silica xerogel and its PEG assisted derivatives were prepared by sol-gel method for controlled release of andrographolide. In vitro and in vivo release of andrographolide from the nano porous silica as well as PEG modified silica matrix were studied. Drug release from the matrix increased with increasing percentage of PEG and followed a biphasic pattern. The in vitro release profile followed zero order kinetics with erosion of the matrix for the first six hours and higuchi model with the diffusion mechanism for rest of the time period. Pharmacokinetic data revealed sustained release of the drug from silica-drug composite with higher elimination t1/2 than the pure drug. For all the formulations Level A in vitro-in vivo correlation (IVIVC) was established with (R2)>0.98. Histology of different organs was carried out following in vivo administration of the drug-carrier composite. The histological examination demonstrated absence of any inflammatory or degenerative response with the formulations. Fourier transform infrared (FTIR) data revealed the coexistence of andrographolide in the silica matrix. Transmission electron microscope (TEM) images also unfold the repose matrix structure in PEG assisted derivatives than the pure matrix. This study discloses that silica gel and PEG assisted derivatives could be used as a biocompatible and sustained release device for andrographolide. © 2014 Elsevier B.V. Source

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