Peerless Hospital And y Research Center

Kolkata, India

Peerless Hospital And y Research Center

Kolkata, India

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PubMed | Peerless Hospital And y Research Center, Burdwan Dental College and Hospital and Dr R Ahmed Dental College And Hospital
Type: Journal Article | Journal: Journal of Indian Society of Periodontology | Year: 2016

Hosts immune response elicits cytokines in response to bacterial challenge. We explore role of one such cytokine interleukin-18 (IL-18) in periodontal health and disease.IL-18 is a pro-inflammatory and tumor suppressive cytokine. Dental literatures suggest that IL-18 might have a role to play in the progression from oral health to periodontal disease. Therefore, this study was undertaken to elucidate the level and role of IL-18 in the gingival crevicular fluid (GCF) and serum of individuals with healthy gingiva, chronic gingivitis, chronic periodontitis, and aggressive periodontitis before and after periodontal therapy.Eighty individuals chosen for the study were divided into healthy control group (1A), chronic gingivitis (2A), chronic periodontitis (3A), and aggressive periodontitis (4A) with twenty individuals each. Criteria for the division were the subjects gingival index, probing pocket depth, clinical attachment loss, and radiographic evidence of bone loss.The individuals underwent treatment (scaling in case of Groups 1A and 2A and scaling and root planing followed by flap surgery in Groups 3A and 4A) to form posttreatment Groups 1B, 2B, 3B, and 4B, respectively. Thus, a total of 160 GCF and 160 serum samples were collected and tested by ELISA.Intergroup comparison was done by post hoc Tukeys test.The mean IL-18 concentration was greatest in Group 3A (GCF 144.61 pg/l, serum 55.12 pg/ml) followed by Group 4A (GCF 98.55 pg/l, serum 39.06 pg/ml), Group 2A (GCF 22.27 pg/l, serum 27.73 pg/ml) and lowest (GCF 17.94 pg/l, serum 11.49 pg/ml) in Group 1A. Posttreatment groups (1B-4B) showed reduction in the mean IL-18 concentration in both GCF and serum.As the inflammation increased, there was a concomitant increase in the level of IL-18 and vice versa following periodontal therapy.

Biswas A.,ICMR Virus Unit | Sarkar K.,ICMR Virus Unit | Firdaus R.,ICMR Virus Unit | Saha K.,ICMR Virus Unit | And 6 more authors.
Asian Journal of Pharmaceutical and Clinical Research | Year: 2016

Objective: The objective was to study the serological prevalence of post-transfusion transmitted infections such as hepatitis C virus (HCV), hepatitis B virus (HBV), and HIV among multi-transfused thalassemic individuals of the Eastern India and the socio and financial difficulties faced by them. Methods: The study was carried out from January 2012 until December 2014 involving 1711 thalassemic major individuals. Blood serum was collected from each patient to perform ELISA for the detection of HBV and HCV seroprevalence. HIV seropositivity along with their hematological and liver function parameters were obtained from the transfusion centers and the host institutions. Other socio-economic conditions were obtained by predesigned proforma of the questionnaire. Results: 67.9% males and 32.1% females were present in our study population of which 75% were from rural area. The mean hemoglobin was found to be lower, whereas mean ferritin, bilirubin, and liver enzymes were much higher than the normal range. Only a handful of 19.76% of the fathers of thalassemic individuals had secondary education. 263 families (15.37%) were familiar with the chances of transfusion-transmitted infections (TTIs). The dominant TTI found within the population was HCV with 18.70% prevalence followed by HIV (3.74%) and HBV (3.33%). 82.93% of the affected families suffered poverty with a meager monthly income within Rs. 5000 fighting against high costs of transfusion and related treatments. Conclusion: Our study reflects the different socio-economic and psychological burdens faced by the thalassemia patients and their families. The high rate of TTIs highlights the need for stringent screening of blood or blood products before administration. © 2016, Asian Journal of Pharmaceutical and Clinical Research. All rights reserved.

PubMed | National Neurosciences Center, Peerless Hospital And y Research Center and Tata Medical Center
Type: Case Reports | Journal: Laboratory medicine | Year: 2015

Vitamin D toxicity also known as hypervitaminosis D was previously believed to be rare. But with an increase in vitamin D supplementation several cases have been reported in literature. Fat soluble vitamins like Vitamin D, due to their ability to accumulate in the body, have a higher potential for toxicity than water soluble vitamins. The main clinical consequence of vitamin D toxicity is hypercalcemia. In this report we describe an adult female patient who developed very high serum Vitamin D levels (746 ng/mL, RI: 20 to 50) as a result of medication error. Inspite of such high serum concentrations the patient was without any clinical symptoms and had normal serum calcium. We critically discuss the mechanism of toxicity and hypothesize the possible molecular/metabolic factors which might have been responsible for this nontoxic presentation. This case study highlights the fact that physicians need to consider the risk of medication errors while prescribing Vitamin D therapy. Clinical trials to study Vitamin D toxicity in humans is not possible ethically. Thus the evidence base regarding the safety profile of Vitamin D supplementation in humans has been build through case reports. This review of the paradoxical clinico-laboratory manifestation of hypervitaminosis D could possibly contribute to existing literature.

Chakraborty S.,Jadavpur University | Biswas S.,Jadavpur University | Sa B.,Jadavpur University | Das S.,Peerless Hospital And y Research Center | Dey R.,Jadavpur University
Colloids and Surfaces A: Physicochemical and Engineering Aspects | Year: 2014

Silica xerogel and its PEG assisted derivatives were prepared by sol-gel method for controlled release of andrographolide. In vitro and in vivo release of andrographolide from the nano porous silica as well as PEG modified silica matrix were studied. Drug release from the matrix increased with increasing percentage of PEG and followed a biphasic pattern. The in vitro release profile followed zero order kinetics with erosion of the matrix for the first six hours and higuchi model with the diffusion mechanism for rest of the time period. Pharmacokinetic data revealed sustained release of the drug from silica-drug composite with higher elimination t1/2 than the pure drug. For all the formulations Level A in vitro-in vivo correlation (IVIVC) was established with (R2)>0.98. Histology of different organs was carried out following in vivo administration of the drug-carrier composite. The histological examination demonstrated absence of any inflammatory or degenerative response with the formulations. Fourier transform infrared (FTIR) data revealed the coexistence of andrographolide in the silica matrix. Transmission electron microscope (TEM) images also unfold the repose matrix structure in PEG assisted derivatives than the pure matrix. This study discloses that silica gel and PEG assisted derivatives could be used as a biocompatible and sustained release device for andrographolide. © 2014 Elsevier B.V.

Biswas S.,Jadavpur University | Chakraborty S.,Jadavpur University | Das S.,Peerless Hospital And y Research Center
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2012

This review aims at compiling the different mutational changes undergone by the factor VIII gene leading to hemophilia A. It has been difficult to characterize completely a genetic disorder like hemophilia A where the gene of concerns is large, its tissue-specific expression, and all affected individuals have different mutational patterns. In this review, point mutational, insertional and deletional patterns are sited. The data are collected from all round the world, although "Western" sources are predominate.

Chakraborty S.,Peerless Hospital and y Research Center | Kallner A.,Karolinska University Hospital
Clinica Chimica Acta | Year: 2015

Introduction: Spurious hyperphosphatemia is a relatively common phenomenon in patients with monoclonal gammopathy which can compromise patient safety. A cost-effective routine method is desirable for laboratories to reduce or eliminate the protein interference. Methods: A protein free sample was obtained after precipitation with PEG-6000 (polyethylene glycol). S-phosphate concentration was measured with routine wet chemistry method in normal and monoclonal gammopathy samples before and after precipitation. Monoclonal samples were also measured by dry chemistry that includes an ultrafiltration step. The protein pattern before and after precipitation was checked by electrophoresis. Results: No effect of PEG precipitation on S-phosphate concentration was demonstrated in normal serum. After PEG precipitation of proteins in monoclonal gammopathies serum wet chemistry gave the same results as dry chemistry i.e. after ultrafiltration. Conclusion: PEG precipitation of proteins offers a cost-effective method to eliminate protein interferences in monoclonal gammopathies. © 2014 Elsevier B.V.

Krishnan P.,Peerless Hospital Complex | Sanyal S.,Peerless Hospital and y Research Center | Das S.,Peerless Hospital and y Research Center
Journal of Craniovertebral Junction and Spine | Year: 2014

Facetal cysts are usually encountered as incidental radiological findings in spinal imaging studies. Only rarely can neurological symptoms be attributed to them. These cysts are lined by vascularized synovial tissue. There are few reports in literature of hemorrhagic transformation in these cysts with sudden increase in size precipitating symptoms acutely. We report one such case where the existence of a hitherto undiagnosed cyst was unmasked by the haemorrhage. There is a need to be aware of this complication in patients with untreated or incidentally diagnosed cysts so that any sudden neurological deterioration can be dealt with promptly.

Chakraborty D.,Peerless Hospital And y Research Center | Basu S.,Peerless Hospital And y Research Center | Das S.,Peerless Hospital And y Research Center
Asian Journal of Pharmaceutical and Clinical Research | Year: 2011

Multidrug resistant (MDR) bacterial infections are rampant throughout the globe where MBLs (Metallo Beta Lactamases) and ESBLs (Extended Spectrum Beta Lactamases) are the major MDR related bacterial enzymes which are being studied widely. In Kolkata, the number of MDR bacteria are gradually increasing, so it has become a major concern to treat patients. Phenotypic and genotypic studies have been conducted with few MDR bacteria to get some idea about them. Both phenotypic (three different methods) and genotypic methods (based on PCR) were utilized to characterize both MBLs and ESBLs of 100 MDR bacterial strains from clinical materials collected from patients admitted in ICCU and ITU of tertiary care hospitals of Kolkata. Other tests like Imipenem hydrolysis and isoelectric focusing tests were also done for confirmation of the findings. Result: In this study it was found that majority of the MBL positive isolates were associated with urinary tract infections. The MBL VIM-2 variety was predominantly found in Pseudomonas aeruginosa. The main ESBL types was CTX-M1. A positive result in phenotypic methods should be confirmed by the genotypic methods as false positive phenotypic results were found in some of the cases. MBL status of isolated organisms from clinical materials collected from intensive care units should be studied at least by phenotypic methods in all hospitals along with their drug resistance pattern.

Chakraborty S.,Peerless Hospital and y Research Center | Sural S.,Peerless Hospital and y Research Center
Annals of Clinical Biochemistry | Year: 2013

Hereditary renal hypouricaemia (HRH) is a genetic disorder commonly associated with exercise-induced acute kidney injury (AKI). We report the case of a 19-year-old man who was admitted to hospital with exercise-induced AKI and who was subsequently shown to have HRH. We believe this to be the first description of a case of HRH co-presenting with rhabdomyolysis.

Nath A.,Peerless Hospital And y Research Center | Basu A.,Peerless Hospital And y Research Center | Basu S.,Peerless Hospital And y Research Center | Gupta S.B.D.,Peerless Hospital And y Research Center | Das S.,Peerless Hospital And y Research Center
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2013

Microorganisms fall continuously on the surface of silicon chips in computers, without showing any significant damage of their surfaces. In this experiment microorganisms collected from air, were allowed to adhere on the surfaces of silicon chips which was compared to a similar situation on plastic surfaces. After a short incubation it was found that microbial adherence on the surface of silicon chips was much less than that on the plastic surfaces.

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