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Hospital de Órbigo, Spain

Mayorga C.,Pediatrics Service | Blazquez A.B.,Research Laboratory for Allergic Diseases | Dona I.,Allergy Service | Gomez F.,Allergy Service | And 6 more authors.
Annals of Allergy, Asthma and Immunology

Background: Delayed-type hypersensitivity to glatiramer acetate is rare, and the underlying immunological mechanisms are not completely understood. Objective: To study the immunologic response in 2 patients with multiple sclerosis who developed maculopapular exanthema related with the administration of glatiramer acetate. Methods: The allergologic study included general blood tests, viral serologic tests, and skin tests (patch and intradermal tests). The immunologic study was performed in skin biopsy specimens by immunohistochemistry and in the peripheral blood by flow cytometry and the lymphocyte transformation test. Results: Skin test results were negative in both patients, and the diagnosis was confirmed by a drug provocation test. The evaluation of the acute phase showed an increase in the percentage of CD8 T lymphocytes (>50%) and the percentage of cells expressing skin-homing receptor (cutaneous lymphocyte-associated antigen) (>70%) and chemokine receptors (CCR4 and CXCR3) at T1. A positive proliferative response was observed in T lymphocytes (stimulation index [SI] = 3.5 in patient 1 and 3.59 in patient 2), especially the CD8 + subpopulation (SI = 5.5 and 4.6 in patients 1 and 2, respectively), and NK lymphocytes (SI = 3.9 and 8.5 in patients 1 and 2, respectively) after glatiramer acetate stimulation. Conclusion: This study demonstrates the important role of T H1 cells expressing skin-homing receptors in delayed-type hypersensitivity reactions to glatiramer acetate. A lymphocyte transformation test revealed a specific glatiramer acetate recognition by T lymphocytes and NK lymphocytes. © 2012 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved. Source

Fernandez-Montero A.,University of Navarra | Olmo-Jimenez J.M.,Public University of Navarra | Olmo N.,Complejo Hospitalario de Jaen | Bes-Rastrollo M.,Public University of Navarra | And 5 more authors.
Preventive Medicine

Objective: Many subjects, especially highly educated subjects, are increasingly exposed to computer use. This exposure might represent an explanation for the growing rates of myopia. Methods: We assessed 17,217 Spanish university graduates from the SUN project, an open-recruitment cohort. Their mean age was 38.5. years (SD 12.1), and their mean time of exposure to computers was 14.3. h/week (SD 14.6). We estimated multivariable-adjusted odds ratios (OR) for the risk of myopia development and/or progression (≥. 0.5 diopters) according to baseline exposure to computer and to changes in exposure. Results: The age and sex-adjusted OR comparing >40h/week of exposure versus. <. 10. h/week was 1.34 (95% confidence interval (CI): 1.12-1.60). This association remained essentially unchanged after additional adjustments. Comparing participants who increased their exposure to computers, versus those with no change, the age and sex-adjusted OR was 1.49 (1.34-1.66). This result was unchanged after additional adjustments. Conclusions: To our knowledge this is the first large longitudinal assessment in young adults, showing that exposure to computer use is associated with myopia development or progression in a cohort of Spanish university graduates. Further studies are needed to confirm these epidemiological findings. © 2014 Elsevier Inc. Source

Arboleya S.,Institute Productos Lacteos Of Asturias Ipla | Sanchez B.,Institute Productos Lacteos Of Asturias Ipla | Milani C.,University of Parma | Duranti S.,University of Parma | And 6 more authors.
Journal of Pediatrics

Objectives To assess the establishment of the intestinal microbiota in very low birthweight preterm infants and to evaluate the impact of perinatal factors, such as delivery mode and perinatal antibiotics. Study design We used 16S ribosomal RNA gene sequence-based microbiota analysis and quantitative polymerase chain reaction to evaluate the establishment of the intestinal microbiota. We also evaluated factors affecting the microbiota, during the first 3 months of life in preterm infants (n = 27) compared with full-term babies (n = 13). Results Immaturity affects the microbiota as indicated by a reduced percentage of the family Bacteroidaceae during the first months of life and by a higher initial percentage of Lactobacillaceae in preterm infants compared with full term infants. Perinatal antibiotics, including intrapartum antimicrobial prophylaxis, affects the gut microbiota, as indicated by increased Enterobacteriaceae family organisms in the infants. Conclusions Prematurity and perinatal antibiotic administration strongly affect the initial establishment of microbiota with potential consequences for later health. © 2015 Elsevier Inc. Source

Da Costa Silva C.,Pediatric Intensive Care Unit | Alves M.M.O.,Pediatrics Service | Dos Santos El Halal M.G.,Federal University of Rio Grande do Sul | Dos Santos Pinheiro S.,Pediatric Intensive Care Unit | Carvalho P.R.A.,Federal University of Rio Grande do Sul
Revista Brasileira de Terapia Intensiva

Objective: Compare the scores resulting from the Comfort-B scale with the bispectral index in children in an intensive care unit. Methods: Eleven children between the ages of 1 month and 16 years requiring mechanical ventilation and sedation were simultaneously classified based on the bispectral index and the Comfort-B scale. Their behavior was recorded using digital photography, and the record was later evaluated by three independent evaluators. Agreement tests (Bland-Altman and Kappa) were then performed. The correlation between the two methods (Pearson correlation) was tested. Results: In total, 35 observations were performed on 11 patients. Based on the Kappa coefficient, the agreement among evaluators ranged from 0.56 to 0.75 (p<0.001). There was a positive and consistent association between the bispectral index and the Comfort-B scale [r=0.424 (p=0.011) to r=0.498 (p=0.002)]. Conclusion: Due to the strong correlation between the independent evaluators and the consistent correlation between the two methods, the results suggest that the Comfort-B scale is reproducible and useful in classifying the level of sedation in children requiring mechanical ventilation. Source

Borras V.,Pediatrics Service | Freitas A.,Autonomous University of Barcelona | Castell C.,Autonomous University of Barcelona | Gispert R.,Autonomous University of Barcelona | Jane M.,Autonomous University of Barcelona
Pediatric Diabetes

Aim: To identify perinatal risk factors associated with the development of type 1 diabetes. Methods: The research was designed as a retrospective study of cases and controls. Catalan Register of Diabetes and the Register of Newborn Screening for Metabolic Diseases were the data sources used in this study. The variables studied include sex, birth weight, age of onset of type 1 diabetes, maternal age at delivery, type of delivery, and type of feeding at birth. Anthropometric growth patterns of Carrascosa have been used to classify birth weight as small for gestational age: weight <10th percentile; appropriate for gestational age: weight ≥10th percentile and ≤90th percentile; and large for gestational age (LGA): weight >90th percentile. Statistical analysis was carried out using bivariate analysis (chi-squared test), and odds ratios and 95% confidence intervals were predicted. Results: A total of 1530 infants were analyzed (306 type 1 diabetes cases, 1224 control subjects). A significantly greater percentage of LGA babies were observed among diabetics (16.3% compared with 11.3% of the control group). The risk of having type 1 diabetes in children who were LGA was 1.45 times greater than that shown in children with normal and low birth weight. Conclusions: Only being born LGA was significantly associated with developing type 1 diabetes. © 2011 John Wiley & Sons A/S. Source

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