Pearl River Fisheries Research Institute

Guangzhou, China

Pearl River Fisheries Research Institute

Guangzhou, China
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Liu Y.,Chinese Academy of Fishery Sciences | Yang R.,Chinese Academy of Fishery Sciences | Liu Y.,Pearl River Fisheries Research Institute | Sun Z.,Beidaihe Central Experiment Station
Journal of the World Aquaculture Society | Year: 2017

This study estimated the heritabilities of growth-related traits in Paralichthys olivaceus using a microsatellite-based pedigree. A set of 48 microsatellite markers located at different regions of each linkage group were selected from the published genetic map. The flounders used here originated from the Bohai Sea and the broodstock was mated to produce 10 full-sib families. The resulting fish were assayed to obtain the probabilities of exclusion (Excl 1 and Excl 2) for each marker. Sixteen markers showed low Excl 1 and Excl 2 values, eight had high probability values, and the remainder showed intermediate probability values. A marker-based pedigree of four full-factorial crosses (FFCs) was constructed using the eight markers with high Excl 1 and Excl 2 probabilities. Body measurements (such as body weight and total length) were determined in 752-943 individuals from the FFCs at 200, 400, and 500d of age. An animal model was used for single-trait analyses and estimated moderate heritabilities, ranging from 0.23 to 0.48. These results demonstrate that markers with high exclusion probabilities provide an efficient tool for parentage assignments and genetic analyses in Japanese flounder. Marker-assisted estimation of heritability is an efficient approach to genetically improve growth traits in this species. © by the World Aquaculture Society 2017.


Hossain M.J.,Auburn University | Sun D.,Auburn University | McGarey D.J.,Kennesaw State University | Wrenn S.,Auburn University | And 5 more authors.
mBio | Year: 2014

Since 2009, catfish farming in the southeastern United States has been severely impacted by a highly virulent and clonal population of Aeromonas hydrophila causing motile Aeromonas septicemia (MAS) in catfish. The possible origin of this newly emerged highly virulent A. hydrophila strain is unknown. In this study, we show using whole-genome sequencing and comparative genomics that A. hydrophila isolates from diseased grass carp in China and catfish in the United States have highly similar genomes. Our phylogenomic analyses suggest that U.S. catfish isolates emerged from A. hydrophila populations of Asian origin. Furthermore, we identified an A. hydrophila strain isolated in 2004 from a diseased catfish in Mississippi, prior to the onset of the major epidemic outbreaks in Alabama starting in 2009, with genomic characteristics that are intermediate between those of the Asian and Alabama fish isolates. Investigation of A. hydrophila strain virulence demonstrated that the isolate from the U.S. catfish epidemic is significantly more virulent to both channel catfish and grass carp than is the Chinese carp isolate. This study implicates the importation of fish or fishery products into the United States as the source of highly virulent A. hydrophila that has caused severe epidemic outbreaks in United States-farmed catfish and further demonstrates the potential for invasive animal species to disseminate bacterial pathogens worldwide. © 2014 Hossain et al.


PubMed | Pearl River Fisheries Research Institute and National University of Singapore
Type: Journal Article | Journal: Journal of fish biology | Year: 2016

In this study, to systematically identify the most stably expressed genes for internal reference in zebrafish Danio rerio investigations, 37 D. rerio transcriptomic datasets (both RNA sequencing and microarray data) were collected from gene expression omnibus (GEO) database and unpublished data, and gene expression variations were analysed under three experimental conditions: tissue types, developmental stages and chemical treatments. Forty-four putative candidate genes were identified with the c.v. <02 from all datasets. Following clustering into different functional groups, 21 genes, in addition to four conventional housekeeping genes (eef1a1l1, b2m, hrpt1l and actb1), were selected from different functional groups for further quantitative real-time (qrt-)PCR validation using 25 RNA samples from different adult tissues, developmental stages and chemical treatments. The qrt-PCR data were then analysed using the statistical algorithm refFinder for gene expression stability. Several new candidate genes showed better expression stability than the conventional housekeeping genes in all three categories. It was found that sep15 and metap1 were the top two stable genes for tissue types, ube2a and tmem50a the top two for different developmental stages, and rpl13a and rp1p0 the top two for chemical treatments. Thus, based on the extensive transcriptomic analyses and qrt-PCR validation, these new reference genes are recommended for normalization of D. rerio qrt-PCR data respectively for the three different experimental conditions.


Liu Y.,Chinese Academy of Fishery Sciences | Yang R.,Chinese Academy of Fishery Sciences | Liu Y.,Pearl River Fisheries Research Institute | Si F.,Beidaihe Central Experiment Station
Journal of the World Aquaculture Society | Year: 2016

Japanese flounder, Paralichthys olivaceus, is an economically important marine fish species in Asia. A suite of 18 microsatellite markers chosen from published genetic linkage maps was used to carry out parentage assignments of 188 hatchery-reared juveniles from a small number of breeders. The probabilities of exclusion for the 18 microsatellite markers were 0.604-0.913, and the effectiveness of combined probability of exclusion reached 100% when using the eight microsatellite markers with higher Excl 1 probabilities. The cultured and wild stocks (WSs) were differentiated in a release-recapture population based on these markers. Of the 321 recaptured offspring, 28.34% were assigned to their parental pairs in our broodstock, whereas the remaining offspring could not be traced back to a possible sire or dam. Significant reduction in genetic diversity of the cultured stock (CS) had not been found compared with that of the WS. The results suggest that CSs released into the wild will not adversely affect the genetic structure of natural populations. Our results demonstrate that these markers provide an efficient tool for parentage assignments and genetic analysis of Japanese flounder. © by the World Aquaculture Society 2016.


Zhang B.,Sun Yat Sen University | Lin W.,Pearl River Fisheries Research Institute | Huang J.,Sun Yat Sen University | Wang Y.,Pearl River Fisheries Research Institute | Xu R.,Sun Yat Sen University
Iranian Journal of Fisheries Sciences | Year: 2010

In order to investigate the effects of artificial substrates (vertical surface of polypropylene fabrics) on cultured shrimp, we reared 28-day old Litopenaeus vannamei post-larvae (PL28) intensively for 90 days at a density of 510 shrimp/m 2 in each of 8 tanks. Two tanks containing no artificial substrate were a control group, and 1, 3 and 5 artificial substrates were present in other 6 tanks. The volume of each tank was 100 L. In the tanks with artificial substrates, the percentage of shrimp distribution on the bottom was less significant (P<0.05) than that in the control tanks. The percentage of shrimps attached to the artificial substrates increased and fewer shrimp occupied the tank bottom as more artificial substrates were added to the tanks. Moreover the trends were more significant as rearing days increased. These results showed that artificial substrates could disperse the shrimp from the tank bottom onto the artificial substrates and thus alleviate the negative effect of high stocking density on shrimp growth in the tanks. Both the average weight and survival in the tanks with artificial substrates were significantly higher (P<0.05) than those in the control tanks. Furthermore, weight and survival increased when more artificial substrates were added. Because the shrimps in all tanks were supplied with suitable water quality and adequate nutritional food, we suggest that the differences of growth and survival were affected mainly by living space added with the addition of artificial substrates.


Bian Q.-Q.,Northwest University, China | Li X.-Y.,Northwest University, China | Fang Y.-Q.,Northwest University, China | Jia Y.-Q.,Northwest University, China | Mu X.-D.,Pearl River Fisheries Research Institute
Mitochondrial DNA | Year: 2015

To identify Pomacea canaliculata and P. insularum using a molecular approach, the partial sequences of mitochondrial (mt) adenosine triphosphate subunit 6 (patp6) genes of two apple snails species from eight provinces of China were obtained. The intra-specific variations in mt patp6 within P. canaliculata were 0-4.5%, and no sequence difference in this region was observed within P. insularum. However, high inter-specific variations between P. canaliculata and P. insularum were detected, with sequence differences of 8.9-10.1%. Phylogenetic analyses based on sequences of mt patp6 revealed that P. canaliculata and P. insularum were grouped in different clades, but the genetic trees could not reveal geographically genetic relationships of P. canaliculata isolates from different origins. These findings will provide basic information for further study of molecular epidemiology and control of Pomacea invasion in China as well as in the world. © 2013 Informa UK Ltd.


Fu X.,Pearl River Fisheries Research Institute | Li N.,Pearl River Fisheries Research Institute | Liu L.,Pearl River Fisheries Research Institute | Lin Q.,Pearl River Fisheries Research Institute | And 6 more authors.
Virus Genes | Year: 2011

Infectious spleen and kidney necrosis virus (ISKNV) is the causative agent of a disease causing high mortality in mandarin fish, Siniperca chuatsi. In this study, complete major capsid protein (MCP) genes of nine ISKNV isolates were sequenced and compared with other known megalocytiviruses to evaluate genetic variation and host range of the viruses. Comparison of nucleotide sequences of MCP gene revealed 92.6-100% identity among nine ISKNV isolates. A phylogenetic tree revealed that 33 megalocytiviruses were divided into three genotypes, and there was a strong host species signal in three genotypes: for genotype I, the host was mainly marine fish; for genotype II, the host was freshwater fish; and for genotype III, the host was mainly flatfish. Nine ISKNV isolates belonged to genotype I or genotype II, suggesting mandarin fish may be a mixing vessel host for megalocytivirus. © Springer Science+Business Media, LLC 2010.


Fu X.,Pearl River Fisheries Research Institute | Li N.,Pearl River Fisheries Research Institute | Lai Y.,Pearl River Fisheries Research Institute | Liu L.,Pearl River Fisheries Research Institute | And 4 more authors.
Fish and Shellfish Immunology | Year: 2012

Infectious spleen and kidney necrosis virus (ISKNV) is the causative agent of a disease causing high mortality and economic losses in mandarin fish, Siniperca chuatsi in China. But little information about vaccine development against ISKNV disease is available. In this study the gene encoding the major capsid protein (MCP), which is predominant structural component of the iridovirus particles, was cloned into a temperature induction prokaryotic expression vector pBV220 and a recombinant protein was detected about 50 kDa in molecular weight and accounted for 23% of total proteins of whole cell. Polyclonal antibodies were raised in rabbits against the purified protein and the reaction of the antibody was confirmed by western blotting using the purified protein and the spleen and kidney of healthy and diseased mandarin fish. The recombinant protein was renatured by dialysis and the juvenile mandarin fish were vaccinated by intraperitoneal injection with recombinant MCP emulsified with ISA 763 adjuvant at a dose of 20 μg/fish, 50 μg/fish and 100 μg/fish, respectively. Specific antibodies and lymphocyte proliferation were detected in three groups and the values of MCP50 group were higher than the other two groups. After challenge infection with ISKNV, fish of MCP50 group showed significantly greater survival than the others and the RPS was 64.3%. In conclusion, the humoral immunity and cellar immunity of mandarin fish were induced by recombinant MCP and the best immune dose was 50 μg/fish. To the best of our knowledge, this is the first time that a recombinant protein vaccine against ISKNV disease was developed in mandarin fish. © 2012.


Li N.,Pearl River Fisheries Research Institute | Yang Z.,Pearl River Fisheries Research Institute | Bai J.,Pearl River Fisheries Research Institute | Fu X.,Pearl River Fisheries Research Institute | And 3 more authors.
Fish and Shellfish Immunology | Year: 2010

The outer membrane protein-OmpK has been considered as a vaccine candidate for the prevention of infections due to Vibrio harveyi, Vibrio alginolyticus and Vibrio parahaemolyticus in fish. Interestingly, the polyclonal antibody raised against the recombinant OmpK from V. harveyi strain EcGs020802 recognized the OmpK homologues from other strains of Vibrio species by immunoblotting. The ompK genes from 19 Vibrio strains including V. harveyi (11), V. alginolyticus (6) and V. parahaemolyticus (2) were then cloned and sequenced. Alignment analysis based on the amino acid sequences indicated that the OmpK from V. harveyi strain EcGs020802 had 71.7-99.2% of identities with those from V. harveyi, V. alginolyticus and V. parahaemolyticus. Western blot analysis revealed that the corresponding native proteins ranged between 28 and 31 kDa, consistent with predicated molecular weight of OmpK in Vibrio strains. Furthermore, the cross-protective property of recombinant OmpK was evaluated through challenge with heterogeneous virulent Vibrio strains in Orange-spotted groupers (Epinephelus coioides). Orange-spotted groupers vaccinated with recombinant OmpK were more tolerant of the infection by virulent Vibrio strains and their relative percentage survival (RPS) was correlative with the degree of the identity of deduced amino acid sequences of their OmpK. Taken together, the OmpK is a conserved protective antigen among tested Vibrio species and might be a potentially versatile vaccine candidate for the prevention of infections due to V. harveyi, V. alginolyticus and V. parahaemolyticus. © 2010 Elsevier Ltd. All rights reserved.


PubMed | Pearl River Fisheries Research Institute
Type: Journal Article | Journal: Fish & shellfish immunology | Year: 2012

Infectious spleen and kidney necrosis virus (ISKNV) is the causative agent of a disease causing high mortality and economic losses in mandarin fish, Siniperca chuatsi in China. But little information about vaccine development against ISKNV disease is available. In this study the gene encoding the major capsid protein (MCP), which is predominant structural component of the iridovirus particles, was cloned into a temperature induction prokaryotic expression vector pBV220 and a recombinant protein was detected about 50kDa in molecular weight and accounted for 23% of total proteins of whole cell. Polyclonal antibodies were raised in rabbits against the purified protein and the reaction of the antibody was confirmed by western blotting using the purified protein and the spleen and kidney of healthy and diseased mandarin fish. The recombinant protein was renatured by dialysis and the juvenile mandarin fish were vaccinated by intraperitoneal injection with recombinant MCP emulsified with ISA 763 adjuvant at a dose of 20g/fish, 50g/fish and 100g/fish, respectively. Specific antibodies and lymphocyte proliferation were detected in three groups and the values of MCP50 group were higher than the other two groups. After challenge infection with ISKNV, fish of MCP50 group showed significantly greater survival than the others and the RPS was 64.3%. In conclusion, the humoral immunity and cellar immunity of mandarin fish were induced by recombinant MCP and the best immune dose was 50g/fish. To the best of our knowledge, this is the first time that a recombinant protein vaccine against ISKNV disease was developed in mandarin fish.

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