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Bian Q.-Q.,Northwest University, China | Li X.-Y.,Northwest University, China | Fang Y.-Q.,Northwest University, China | Jia Y.-Q.,Northwest University, China | Mu X.-D.,Pearl River Fisheries Research Institute
Mitochondrial DNA | Year: 2015

To identify Pomacea canaliculata and P. insularum using a molecular approach, the partial sequences of mitochondrial (mt) adenosine triphosphate subunit 6 (patp6) genes of two apple snails species from eight provinces of China were obtained. The intra-specific variations in mt patp6 within P. canaliculata were 0-4.5%, and no sequence difference in this region was observed within P. insularum. However, high inter-specific variations between P. canaliculata and P. insularum were detected, with sequence differences of 8.9-10.1%. Phylogenetic analyses based on sequences of mt patp6 revealed that P. canaliculata and P. insularum were grouped in different clades, but the genetic trees could not reveal geographically genetic relationships of P. canaliculata isolates from different origins. These findings will provide basic information for further study of molecular epidemiology and control of Pomacea invasion in China as well as in the world. © 2013 Informa UK Ltd.

Zhang B.,Sun Yat Sen University | Lin W.,Pearl River Fisheries Research Institute | Huang J.,Sun Yat Sen University | Wang Y.,Pearl River Fisheries Research Institute | Xu R.,Sun Yat Sen University
Iranian Journal of Fisheries Sciences | Year: 2010

In order to investigate the effects of artificial substrates (vertical surface of polypropylene fabrics) on cultured shrimp, we reared 28-day old Litopenaeus vannamei post-larvae (PL28) intensively for 90 days at a density of 510 shrimp/m 2 in each of 8 tanks. Two tanks containing no artificial substrate were a control group, and 1, 3 and 5 artificial substrates were present in other 6 tanks. The volume of each tank was 100 L. In the tanks with artificial substrates, the percentage of shrimp distribution on the bottom was less significant (P<0.05) than that in the control tanks. The percentage of shrimps attached to the artificial substrates increased and fewer shrimp occupied the tank bottom as more artificial substrates were added to the tanks. Moreover the trends were more significant as rearing days increased. These results showed that artificial substrates could disperse the shrimp from the tank bottom onto the artificial substrates and thus alleviate the negative effect of high stocking density on shrimp growth in the tanks. Both the average weight and survival in the tanks with artificial substrates were significantly higher (P<0.05) than those in the control tanks. Furthermore, weight and survival increased when more artificial substrates were added. Because the shrimps in all tanks were supplied with suitable water quality and adequate nutritional food, we suggest that the differences of growth and survival were affected mainly by living space added with the addition of artificial substrates.

Liu Y.,Chinese Academy of Fishery Sciences | Yang R.,Chinese Academy of Fishery Sciences | Liu Y.,Pearl River Fisheries Research Institute
Journal of the World Aquaculture Society | Year: 2016

Japanese flounder, Paralichthys olivaceus, is an economically important marine fish species in Asia. A suite of 18 microsatellite markers chosen from published genetic linkage maps was used to carry out parentage assignments of 188 hatchery-reared juveniles from a small number of breeders. The probabilities of exclusion for the 18 microsatellite markers were 0.604-0.913, and the effectiveness of combined probability of exclusion reached 100% when using the eight microsatellite markers with higher Excl 1 probabilities. The cultured and wild stocks (WSs) were differentiated in a release-recapture population based on these markers. Of the 321 recaptured offspring, 28.34% were assigned to their parental pairs in our broodstock, whereas the remaining offspring could not be traced back to a possible sire or dam. Significant reduction in genetic diversity of the cultured stock (CS) had not been found compared with that of the WS. The results suggest that CSs released into the wild will not adversely affect the genetic structure of natural populations. Our results demonstrate that these markers provide an efficient tool for parentage assignments and genetic analysis of Japanese flounder. © by the World Aquaculture Society 2016.

Huang J.,Sun Yat Sen University | Lin W.,Pearl River Fisheries Research Institute | Shi C.,Pearl River Fisheries Research Institute | Wu S.,Pearl River Fisheries Research Institute | Xu R.,Sun Yat Sen University
Aquaculture International | Year: 2010

By use of the polyurethane foam unit (PF unit) method, structural and functional data for protozoan communities were assessed as indicators of the effect of restoration techniques in mandarin fish culture ponds. A total of 165 species of protozoa were observed, including 76 species of ciliates, 57 species of phytomastigophorans, 14 species of zoomastigophorans, and 18 species of sarcodinas. In June, 97 protozoan species colonized the PF units. In August, the number of colonizing species was only 78. In November, a total of 139 species were recorded. In June and August the co-dominant species of protozoans were mainly heterotrophic creatures which feed on bacteria and organic detritus. In November, the co-dominant species were mostly phytoflagelates. The average DO value in the control pond was very low at all times throughout the survey. Removing the sediment, changing the water, and adding compound microbiological preparation had substantial and important effects on reducing total ammonium nitrogen and nitrite nitrogen. Compared with the control ponds, the numbers of protozoan species colonizing the experimental ponds reached its maximum level more slowly and the protozoan community pollution values were lower. The dynamic characteristics of the protozoan colonization process and community pollution values were in agreement with water eutrophic status and could be used for efficient monitoring of the effects of restoration techniques. © 2009 Springer Science+Business Media B.V.

Shuai F.,Pearl River Fisheries Research Institute | Shuai F.,Key Laboratory of the Conservation and Ecological Restoration of Fishery Resource in the Pearl River | Shuai F.,University Paul Sabatier | Li X.,Pearl River Fisheries Research Institute | And 8 more authors.
PLoS ONE | Year: 2016

Knowledge of temporal patterns of larval fish occurrence is limited in south China, despite its ecological importance. This research examines the annual and seasonal patterns of fish larval presence in the large subtropical Pearl River. Data is based on samples collected every two days, from 2006 to 2013. In total, 45 taxa representing 13 families and eight orders were sampled. The dominant larval family was Cyprinidae, accounting for 27 taxa. Squaliobarbus curriculus was the most abundant species, followed by Megalobrama terminalis, Xenocypris davidi, Cirrhinus molitorella, Hemiculter leuscisculus and Squalidus argentatus. Fish larvae abundances varied significantly throughout the seasons (multivariate analyses: Cluster, SIMPROF and ANOSIM). The greatest numbers occurred between May and September, peaking from June through August, which corresponds to the reproductive season. In this study, redundancy analysis was used to describe the relationship between fish larval abundance and associated environmental factors. Mean water temperature, river discharge, atmospheric pressure, maximum temperature and precipitation play important roles in larval occurrence patterns. According to seasonal variations, fish larvae occurrence is mainly affected by water temperature. It was also noted that the occurrence of Salanx reevesii and Cyprinus carpio larvae is associated with higher dissolved oxygen (DO) concentrations, higher atmospheric pressure and lower water temperatures which occur in the spring. On the other hand, M. terminalis, X. davidi, and C. molitorella are associated with high precipitation, high river discharge, low atmospheric pressure and low DO concentrations which featured during the summer months. S. curriculus also peaks in the summer and is associated with peak water temperatures and minimum NH3-N concentrations. Rhinogobius giurinus occur when higher atmospheric pressure, lower precipitation and lower river discharges occur in the autumn. Dominant fish species stagger their spawning period to avoid intraspecific competition for food resources during early life stages; a coexistence strategy to some extent. This research outlines the environmental requirements for successful spawning for different fish species. Understanding processes such as those outlined in this research paper is the basis of conservation of fish community diversity which is a critical resource to a successful sustainable fishery in the Pearl River. © 2016 Shuai et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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