PDVVPFs Medical College

Ahmadnagar, India

PDVVPFs Medical College

Ahmadnagar, India
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Ghodake S.R.,PDVVPFs Medical College | Ghodake S.R.,K B N Institute Of Medical Science | Suryakar A.N.,Dr V M Government Medical College | Ankush R.D.,Dr V M Government Medical College | And 3 more authors.
Indian Journal of Nephrology | Year: 2011

Nephrotic syndrome (NS) is characterized by heavy proteinuria and hypoalbuminuria. Reactive oxygen species (ROS) seem to play an important role in the etiopathogenesis of proteinuria in NS. This study aims to evaluate the potential role of reactive oxygen species in pathogenesis of NS by estimating the levels of oxidants and antioxidants in children with NS. Thirty patients of NS and thirty age, sex-matched healthy subjects, were selected for the study. As compared to healthy controls, the levels of serum lipid peroxide were significantly elevated while levels of nitric oxide, erythrocyte-superoxide dismutase activity, levels of vitamin C, albumin and total antioxidant capacity were significantly reduced in nephrotic patients.The levels of uric acid and bilirubin were significantly increased in children with NS as compared to controls. There was no significant difference in vitamin E level between patients and controls. It can be concluded that increased ROS generation and decreased antioxidant defense may be related to the pathogenesis of proteinuria in NS.


Hundekar P.S.,ACPM Medical College | Suryakar A.N.,Maharashtra University of Health Sciences | Karnik A.C.,ACPM Medical College | Valvi R.,Civil Hospital and Medicare Hospital | And 2 more authors.
Journal of Clinical and Diagnostic Research | Year: 2012

Background: Sickle cell anaemia is a hereditary disorder, associated with severe haemolytic anaemia, periodical vasoocclusive pain and premature death. Oxidative stress is one of the factors that may enhance the rate of haemolysis by damaging the erythrocyte membrane by lipid peroxidation. Aim: The present study was carried out to investigate the oxidant and antioxidant status in sickle cell individuals and the effect of antioxidant supplementation on oxidative stress. Material and Method: A total of 90 subjects participated in the study, including 30 heterozygous (HbAS) and 30 homozygous (HbSS) sickle cell patients and 30 age and sex matched healthy controls. Oxidative stress was evaluated by measuring the levels of serum malondialdehyde (MDA), plasma protein carbonyl, serum nitric oxide (NO), the erythrocytic activity of superoxide dismutase (SOD) and catalase and the total antioxidant capacity (TAC) of plasma before and one month after of antioxidant supplementation. Results: The baseline levels of MDA, protein carbonyl, NO and the activity of SOD were significantly (p<0.001) elevated in the HbSS and HbAS groups as compared to those of the controls. The baseline level of the activity of catalase and the TAC of plasma were significantly (p<0.001) decreased in the HbSS and HbAS groups as compared to those in the controls. After the supplementation of the antioxidants, we found a significant (p<0.001) decrease in the levels of MDA, protein carbonyl, NO and in the activity of SOD, while there was a significant (p<0.001) increase in the level of activity of catalase and in the TAC of plasma in both the groups of sickle cell patients. Conclusion: The values of both the oxidants and the antioxidants did not meet that of the controls, thus suggesting a spontaneous generation of free radicals that consumed the antioxidants. Therefore, antioxidant supplementation is essential in sickle cell individuals in the steady state as well as in illness, to prevent the oxidative damage to the erythrocytes.


Bhagat S.S.,PDVVPFs Medical College | Sarkar P.D.,MGM Medical College | Suryakar A.N.,Maharashtra University of Health Sciences | Padalkar R.K.,PDVVPFs Medical College | And 3 more authors.
Indian Journal of Physiology and Pharmacology | Year: 2013

It has been anticipated that iron and ferritin burden in patients with beta thalassemia major is associated with enhanced free radical formation and blemished antioxidant defense system. The goal of study was to scrutinize impact of serum iron, total iron binding capacity (TIBC), ferritin and erythrocyte catalase in patients with beta thalassemia major. 140 beta thalassemia major patients were studied before and after supplementation of antioxidants for one month, and status was compared with 140 age and sex matched healthy controls. A significant elevation was found in the levels of serum iron and ferritin (P<0.001) with concomitant decrease in erythrocyte catalase (P<0.001) in patients when compared with controls. After one month supplementation of antioxidants, catalase was elevated significantly (P<0.001) and marginal rise in serum TIBC concentration increased marginally while iron and ferritin were decreased marginally (P>0.05) when compared with controls and baselines values. Beta thalassemia major children receive multiple blood transfusions, and are at risk of secondary iron overload induced oxidative stress. These effects may be help to minimize with supplementation of antioxidants.


Bhagat S.S.,PDVVPFs Medical College | Sarkar P.D.,P.A. College | Suryakar A.N.,Maharashtra University of Health Sciences | Padalkar R.K.,PDVVPFs Medical College | And 4 more authors.
Journal of Clinical and Diagnostic Research | Year: 2012

Background: β - thalassemia major is genetic disorder which is caused by mutations in the HBB gene which is on chromosome 11. It is associated with a profound anaemia which is characterized by extreme pallor, jaundice or a failure to thrive, which is accompanied by poor feeding, irritability, decreased activity or an increased somnolence. Hepatosplenomegaly, an expanded bone marrow, siderosis, cardiomegaly, an impaired erythropoiesis, haemolysis in the peripheral circulation and the deposition of excess iron in the tissues are usually present. It has been proposed that the iron overload in the patients with beta thalassaemia major is associated with enhanced free radical formation and defects in the antioxidant defense system. Excess iron facilitates the generation of free radicals, which is the main cause of the tissue damage in the biological system. Aim: The aim of this study was to examine the impact of the iron overload in the patients with beta thalassaemia major on the markers of oxidative stress, which included Malondialdehyde (MDA), which was a breakdown product of lipid peroxidation, Erythrocytic Superoxide Dismutase (ESOD) and vitamin E. Material and Methods: A total of 120 subjects were assessed, who included 60 beta thalassaemia major patients before and after they were supplemented with antioxidants for one month, and their status was compared with those of 60 age and sex matched healthy controls. The levels of serum MDA was analyzed by the Kei Satoh method, serum iron was analyzed by the dipyridyl method, ESOD was analyzed by the Kajari Das method and the vitamin E concentration was measured by the Baker and Frank method. Results: The serum levels of MDA, ESOD and iron were found to be significantly increased (p<0.001) and the serum vitamin E level was significantly decreased (p< 0.001) in the beta thalassaemia major patients as compared to those in the healthy controls. After the supplementation of the antioxidants, we found significantly lower (p<0.001) mean values of serum MDA, iron and ESOD, while the activity of the serum level of vitamin E was significantly higher (p<0.001) as compared to those in the healthy controls. Conclusion: Repeated blood transfusions result in excessive free iron concentration in the blood, which causes increased oxidative stress and the generation of free radicals. The increased oxidative damage in thalassaemia major may be due to the depletion of lipid soluble antioxidants such as vitamin E. These effects may be minimized with the supplementation of antioxidants.


Ghone R.A.,PDVVPFs Medical College | Suryakar A.N.,Maharashtra University of Health Sciences | Kulhalli P.M.,Maharashtra University of Health Sciences | Bhagat S.S.,PDVVPFs Medical College | And 4 more authors.
Journal of Clinical and Diagnostic Research | Year: 2013

Background: Malnutrition represents one of the most severe health problems in India. Free radicals play an important role in immunological response, which induces the oxidative surplus in severe acute malnutrition. Severe dietary deficiency of nutrients leads to increased oxidative stress in cellular compartments. Aim: The goal of this study was to inspect impact of oxidative stress in the form of serum malondialdehyde as product of lipid peroxidation, vitamin E, zinc and erythrocyte superoxide dismutase in patients with severe acute malnutrition. Material and Methods: Sixty severe acute malnutrition patients were studied before and after supplementation of antioxidants for one month, and their status were compared with those of 60 age and sex matched healthy controls. The level of serum MDA was analyzed by the Kei Satoh method, serum vitamin E concentration was measured by Baker and Frank Method, serum zinc was measured by using Atomic Absorption Spectrophotometer (AAS) and erythrocyte superoxide dismutase was measured by Kajari Das Method. Results: Significantly increased levels of serum malondialdehyde (p<0.001) were found in the patients as compared to those in controls, and significant depletions were found in the levels of serum vitamin E, zinc and erythrocyte superoxide dismutase in patients with severe acute malnutrition as compared to those in controls. After supplementation of antioxidants for one month, the levels of malondialdehyde were found to be decreased significantly (p<0.001) and zinc and erythrocyte superoxide dismutase capacity levels were increased significantly (p<0.05). Also, there was a non-significant (p>0.05) increase in vitamin E levels as compared to those before supplementation results. Conclusion: Harsh deficiency of various nutrients in severe acute malnutrition leads to generation of heavy oxidative stress. These effects may be minimized with supplementation of antioxidants.


Patil S.M.,PDVVPFs Medical College | Banker M.P.,Bj Medical College | Padalkar R.K.,Swasthya Hospital and Research Center | Pathak A.P.,PDVVPFs Medical College | And 3 more authors.
Journal of Clinical and Diagnostic Research | Year: 2013

Background: An early identification of the patients with the Acute Coronary Syndrome (ACS) is of prime importance, due to the associated very high mortality. Only about 22% of the patients who present at the emergency cardiology care centres with chest pain, have coronary disease. Ischaemia modified albumin has already been licensed by the US Food and Drug Administration for the diagnosis of suspected myocardial ischaemia. Aim: The goal of the present study was to assess the diagnostic value of serum ischaemia modified albumin and to compare it with sensitive cardiac troponin I in patients with the acute coronary syndromes like unstable angina and acute myocardial infarction. Methods: A diagnostic case control study was conducted on 102 patients who presented to the Emergency Department within 6 hrs of having acute chest pain and on 110 healthy age and sex matched volunteers who formed the control group. The serum Ischaemia Modified Albumin level was estimated by the albumin cobalt binding test by using a digital spectrophotometer, while Troponin I was measured by doing an immunofluroscence assay. A receiver operating characteristic curve was established for ischaemia modified albumin, to determine the cut-off point.


PubMed | PDVVPFs Medical College
Type: Journal Article | Journal: Journal of clinical and diagnostic research : JCDR | Year: 2013

Malnutrition represents one of the most severe health problems in India. Free radicals play an important role in immunological response, which induces the oxidative surplus in severe acute malnutrition. Severe dietary deficiency of nutrients leads to increased oxidative stress in cellular compartments.The goal of this study was to inspect impact of oxidative stress in the form of serum malondialdehyde as product of lipid peroxidation, vitamin E, zinc and erythrocyte superoxide dismutase in patients with severe acute malnutrition.Sixty severe acute malnutrition patients were studied before and after supplementation of antioxidants for one month, and their status were compared with those of 60 age and sex matched healthy controls. The level of serum MDA was analyzed by the Kei Satoh method, serum vitamin E concentration was measured by Baker and Frank Method, serum zinc was measured by using Atomic Absorption Spectrophotometer (AAS) and erythrocyte superoxide dismutase was measured by Kajari Das Method.Significantly increased levels of serum malondialdehyde (p<0.001) were found in the patients as compared to those in controls, and significant depletions were found in the levels of serum vitamin E, zinc and erythrocyte superoxide dismutase in patients with severe acute malnutrition as compared to those in controls. After supplementation of antioxidants for one month, the levels of malondialdehyde were found to be decreased significantly (p<0.001) and zinc and erythrocyte superoxide dismutase capacity levels were increased significantly (p<0.05). Also, there was a non-significant (p>0.05) increase in vitamin E levels as compared to those before supplementation results.Harsh deficiency of various nutrients in severe acute malnutrition leads to generation of heavy oxidative stress. These effects may be minimized with supplementation of antioxidants.

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