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Bahādurgarh, India

Bhatia S.,PDMCOP | Sharma K.,Jamia Hamdard University | Nagpal K.,Sudan University of Science and Technology | Bera T.,Jadavpur University
Bioactive Carbohydrates and Dietary Fibre | Year: 2015

In the present study various extraction methodologies have been adopted to derive sulfated polysaccharides (SPs) called as porphyran (POR) from low tide (LT) and high tide (HT) samples of Porphyra vietnamensis. Initially various taxonomical differences among LT and HT samples of P. vietnamensis were evaluated. Later on various physico-chemical and antifungal properties were determined. Our results suggested that P. vietnamensis collected during LT yields high percentage of SPs with relatively low molecular weight (MW) and high sulfate content than the HT sample. Among various extraction methodologies alkali modified POR yields low MW polysaccharide but surprisingly with high sulfate content which have shown improved physico-chemical and antifungal properties than chitosan (CS) without any toxicological affects. However, extensive research is further required to explore the molecular pathways inhibited or stimulated in this process. © 2015 Published by Elsevier Ltd. Source

Bhatia S.,PDMCOP | Kumar V.,PDMCOP | Sharma K.,Jamia Hamdard University | Nagpal K.,Lovely Professional University | Bera T.,Jadavpur University
Scientific World Journal | Year: 2014

Development of oral amphotericin B (AmB) loaded nanoparticles (NPs) demands a novel technique which reduces its toxicity and other associated problems. Packing of AmB in between two oppositely charged ions by polyelectrolyte complexation technique proved to be a successful strategy. We have developed a novel carrier system in form of polyelectrolyte complex of AmB by using chitosan (CS) and porphyran (POR) as two oppositely charged polymers with TPP as a crosslinking agent. Initially POR was isolated from Porphyra vietnamensis followed by the fact that its alkali induced safe reduction in molecular weight was achieved. Formulation was optimized using three-factor three-level (33) central composite design. High concentration of POR in NPs was confirmed by sulfated polysaccharide (SP) assay. Degradation and dissolution studies suggested the stability of NPs over wide pH range. Hemolytic toxicity data suggested the safety of prepared formulation. In vivo and in vitro antifungal activity demonstrated the high antifungal potential of optimized formulation when compared with standard drug and marketed formulations. Throughout the study TPP addition did not cause any significant changes. Therefore, these experimental oral NPs may represent an interesting carrier system for the delivery of AmB. © 2014 Saurabh Bhatia et al. Source

Bhatia S.,PDMCOP | Rathee P.,PDMCOP | Sharma K.,Jamia Hamdard University | Chaugule B.B.,University of Pune | And 2 more authors.
International Journal of Biological Macromolecules | Year: 2013

Our investigation explores the immuno-efficiency of sulphated polysaccharides enriched Porphyra vietnamenis. Isolated polysaccharide fraction (17.1-25.8%) was characterized by FTIR and NMR spectroscopy which showed the presence of typical linear backbone structure called as porphyran. Oral administration of porphyran (200-500. mg/kg) evoked a significant (P= 0.05) increase in weight of the thymus, spleen and lymphoid organ cellularity. The total leucocyte and lymphocyte count was increased significantly (P< 0.005). The increase in the percent neutrophil adhesion to nylon fibres as well as a dose-dependent increase in antibody titre values was observed. A decreased response to DTH reaction induced by SRBC was recorded. A potential phagocytic response was seen and significant changes were observed in the formation of formazone crystals. It also prevented myelosuppression in cyclophosphamide drug treated rats. The results indicated that P. vietnamenis possesses potential immunomodulatory activity and has therapeutic potential for the prevention of autoimmune diseases. © 2013 Elsevier B.V. Source

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