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Lahore, Pakistan

Tahir L.,Quaid-i-Azam University | Tahir L.,PCSIR Labs Complex | Ali M.I.,Quaid-i-Azam University | Zia M.,Quaid-i-Azam University | And 3 more authors.
Polish Journal of Microbiology | Year: 2013

Polystyrene is considered stable to biological degradation. Lantinus tigrinus isolated from wood sample produced esterase in growth medium under normal conditions. However, acidic medium, 37°C temperature, presence of tween 80; and urea and yeast extract in mineral salt medium enhance the production of esterase and specific activity. Purified esterase was active at broad pH range and 45°C. FTIR analysis confirmed that esterase produced by Lantinus tigrinus effectively degraded polystyrene film and broke macromolecules down to non-toxic molecules. This study concludes that the presence of Lantinus tigrinus at dumping sites can be exploited for waste management containing high molecular weight synthetic polymers.

Khan K.M.,University of Karachi | Rahim F.,University of Karachi | Ambreen N.,University of Karachi | Taha M.,University of Karachi | And 3 more authors.
Journal of the Chemical Society of Pakistan | Year: 2012

An efficient and simplified protocol for three component Mannich reaction of indoles in water without using any catalyst is described. Different functionalized indole Mannich base synthesized in relatively short time with moderate to good yields. The advantages of this methodology are higher selectivity, simplicity, and shorter reaction time. To the best of our knowledge this is the first green protocol for indole Mannich base reaction.

Abd Ei-Salam N.M.,King Saud University | Hussain I.,University of Peshawar | Ullah R.,Sarhad University of Science and Information Technology | Shanzeb,Kohat University of Science and Technology | And 2 more authors.
Life Science Journal | Year: 2013

Eclipta prostrata, is an important medicinal plant and their different parts areused for different health disorders. The triterpenoids isolated E. prostrata displayed antiproliferative and antimicrobial potentials. Phytochemically, E. prostrata is rich in wadeoloctone, eclalbasaponin, b- amyrin, stigmasterol and luteolin-7- glucoside. Keeping in view the importance of E prostrata, it was analyzed quantitatively, qualitatively for the phytochemicals (alkaloids, flavonoids, saponins, tannins, glycosides, terpenoids, reducing sugars, anthraquinones, and cardiacglycoside) Physiochemicals, and anti-fungal activity. For anti-fungal activity four different strains including Aspergillus niger, Aspergillus fumigatus, Fusarium solani and Aspergillus flavus used has shown very promising results against the fungal strains.

Hussain I.,Kohat University of Science and Technology | Khan L.,PCSIR Labs Complex | Marwat G.A.,PCSIR Labs Complex
Journal of the Chemical Society of Pakistan | Year: 2011

Vitamin C or ascorbic acid, a very useful constituent of redox mechanism is used in medicine and also added in manufactured food for anti-oxidation. A UV-spectrophotometric method was used for the determination of Vitamin C in 4 different medicinal plants. High amount of Vitamin C 160 mg/100 g was found in Citrulus colcocynthis, followed by Hippophae rhamonides oil 136.1 mg/100g. A relatively low concentration of Vitamin C was recorded in Glycyrhiza glabra 56.2 mg/100g and Withinia somnifera 51.50 mg/100 g. The presence of high concentration of Vitamin C in selected medicinal plants might be responsible for their therapeutic effects and uses in the traditional system of medicine.

Ur Rahman K.,University of Peshawar | Imdadullahmohammadzai,University of Peshawar | Hussain A.,PCSIR Labs Complex | Ullah S.,PCSIR Labs Complex
Middle East Journal of Scientific Research | Year: 2013

The metabolic extract of branded and unbranded honey samples were evaluated for their scavenging activity of DPPH free radical by using different concentrations (100, 200, 300, 500 and 600 μg/ml) of honey samples. The antioxidant activity of the extracts were determined against 1,1 -diphenyl-2-picryl hydroxyl (DPPH) by spectrophotometer. Sample 4 showed maximum antioxidant activity (84.33±1.23) at the concentration 600 μg/ml, whereas the lowest activity (24.12±1.17) was observed at the concentration 100 μg/ml in sample 3. In case of unbranded honey, sample 4 showed maximum antioxidant activity (81.26±1.44) at the concentration 600 μg/ml among all honey samples, whereas the lowest activity (20.22±1.19) was observed at the concentration 100 μg/ml in sample 1. As the concentration of these compounds increased the percent scavenging activity also increased. It is concluded from this study that all the branded and unbranded honey samples evaluated showed antioxidant activity. Branded samples presented better activity as compared to unbranded samples. Thus specifically honey could be used as alternative natural antioxidant in different formulations for food and pharmaceutical industries. © IDOSI Publications, 2013.

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