PCSIR Laboratories Complex Karachi

Karachi, Pakistan

PCSIR Laboratories Complex Karachi

Karachi, Pakistan
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Arshad T.,Jinnah University for Women | Khan K.M.,University of Karachi | Rasool N.,Jinnah University for Women | Salar U.,University of Karachi | And 9 more authors.
Bioorganic Chemistry | Year: 2017

On the basis of previous report on promising α-glucosidase inhibitory activity of 5-bromo-2-aryl benzimidazole derivatives, these derivatives were further screened for urease inhibitory and cytotoxicity activity in order to get more potent and non-cytotoxic potential dual inhibitor for the patients suffering from diabetes as well as peptic ulcer. In this study, all compounds showed varying degree of potency in the range of (IC50 = 8.15 ± 0.03–354.67 ± 0.19 μM) as compared to standard thiourea (IC50 = 21.25 ± 0.15 μM). It is worth mentioning that derivatives 7 (IC50 = 12.07 ± 0.05 μM), 8 (IC50 = 10.57 ± 0.12 μM), 11 (IC50 = 13.76 ± 0.02 μM), 14 (IC50 = 15.70 ± 0.12 μM) and 22 (IC50 = 8.15 ± 0.03 μM) were found to be more potent inhibitors than standard. All compounds were also evaluated for cytotoxicity towards 3T3 mouse fibroblast cell line and found to be completely non-toxic. Previously benzimidazole 1–25 were also showed α-glucosidase inhibitory potential. In silico studies were performed on the lead molecules i.e. 2, 7, 8, 11, 14, and 22, in order to rationalize the binding interaction of compounds with the active site of urease enzyme. © 2017 Elsevier Inc.


Sher N.,University of Karachi | Fatima N.,University of Karachi | Perveen S.,PCSIR Laboratories Complex Karachi | Siddiqui F.A.,University of Karachi | Wafa Sial A.,Ziauddin University
International Journal of Analytical Chemistry | Year: 2015

This paper demonstrates colorimetric visible spectrophotometric quantification methods for amino acid, namely, tranexamic acid and pregabalin. Both drugs contain the amino group, and when they are reacted with 2,4-dinitrophenol and 2,4,6-trinitrophenol, they give rise to yellow colored complexes showing absorption maximum at 418 nm and 425 nm, respectively, based on the Lewis acid base reaction. Detailed optimization process and stoichiometric studies were conducted along with investigation of thermodynamic features, that is, association constant and standard free energy changes. The method was linear over the concentration range of 0.02-200 μgmL-1 with correlation coefficient of more than 0.9990 in all of the cases. Limit of detection was in range from 0.0041 to 0.0094 μgmL-1 and limit of quantification was in the range from 0.0137 to 0.0302 μgmL-1. Excellent recovery in Placebo spiked samples indicated that there is no interference from common excipients. The analytical methods under proposal were successfully applied to determine tranexamic acid and pregabalin in commercial products. t -test and F ratio were evaluated without noticeable difference between the proposed and reference methods. © 2015 Nawab Sher et al.


PubMed | Ziauddin University, University of Karachi and PCSIR Laboratories Complex Karachi
Type: | Journal: International journal of analytical chemistry | Year: 2015

This paper demonstrates colorimetric visible spectrophotometric quantification methods for amino acid, namely, tranexamic acid and pregabalin. Both drugs contain the amino group, and when they are reacted with 2,4-dinitrophenol and 2,4,6-trinitrophenol, they give rise to yellow colored complexes showing absorption maximum at 418nm and 425nm, respectively, based on the Lewis acid base reaction. Detailed optimization process and stoichiometric studies were conducted along with investigation of thermodynamic features, that is, association constant and standard free energy changes. The method was linear over the concentration range of 0.02-200gmL(-1) with correlation coefficient of more than 0.9990 in all of the cases. Limit of detection was in range from 0.0041 to 0.0094gmL(-1) and limit of quantification was in the range from 0.0137 to 0.0302gmL(-1). Excellent recovery in Placebo spiked samples indicated that there is no interference from common excipients. The analytical methods under proposal were successfully applied to determine tranexamic acid and pregabalin in commercial products. t-test and F ratio were evaluated without noticeable difference between the proposed and reference methods.


Yousuf S.,University of Karachi | Khan K.M.,University of Karachi | Naz F.,University of Karachi | Perveen S.,PCSIR Laboratories Complex Karachi | Miana G.A.,A And G Pharmaceutical, Inc.
Acta Crystallographica Section E: Structure Reports Online | Year: 2013

In the molecule of the title compound, C7H9N 3O3, the nitro and carbonyl groups are tilted with respect to the imidazole ring by 9.16 (6) and 65.47 (7)°, respectively. Neighbouring chains are linked via C-H⋯N and C-H⋯O hydrogen bonds forming two-dimensional slab-like networks lying parallel to (01-1).


Khan K.M.,University of Karachi | Khan M.,University of Karachi | Khan M.,Abdul Wali Khan University Mardan | Khan A.,University of Karachi | And 3 more authors.
Journal of the Chemical Society of Pakistan | Year: 2014

Earlier 5-arylidene N,N-dimethylbarbiturates 1-24 were reported by our group as novel antioxidant, nevertheless, urease inhibitory activity of these compounds has not been investigated at all. In the present study, compounds 1-24 were synthesized by the reaction of N,N-dimethylbarbituric acid with different aromatic aldehydes, and screened for their in vitro urease inhibitory effects. Compound 7, was found to be most active urease inhibitor in the series. Nonetheless, compounds 11, 12, and 19 were also active against urease enzyme.


Bi F.,PCSIR Laboratories Complex Karachi | Iqbal S.,PCSIR Laboratories Complex Karachi | Arman M.,PCSIR Laboratories Complex Karachi | Ali A.,PCSIR Laboratories Complex Karachi | Hassan M.-U.,PCSIR Laboratories Complex Karachi
Journal of Saudi Chemical Society | Year: 2011

The effects of polysaccharide elicitor k-carrageenan obtained from Hypnea musciformis, red algae on the production of Induced Secondary Metabolites, ISMs (the disease resistance compounds) and on various growth characters of chickpea and maize plants were studied. Experiments were conducted in the field of PCSIR Laboratories Complex Karachi during December 2008-April 2009 in randomized complete block design with three replications. Three elicitor treatments were used, a solid preparation in which the elicitor was mixed with soil (T 2 1mg/g) and applied around the seeds in the soil. The two other preparations were liquid, T 1 and T 3 at a concentration of 100μgglceqml -1 and were applied around the sowing seeds and as a foliar spray on the plants, respectively. Statistical analysis of the data revealed that these treatments significantly enhanced all the growth characters of chickpea except T 2 that gave the nonsignificant difference in the plant height. Maximum plant height (80.3cm), number of pods plant -1 (76.2), number of branches plant -1 (25.0), number of leaves plant -1 (125.6), earlier flowering and high ISMs contents in leaves, stem and grains of chickpea were recorded in T 1 treated chickpea plants. In maize plants only T 1 and T 3 treatments (with minor exceptions) had significant effects on few characters like plant height, stem diameter, number of leaves plant -1 and on ISMs contents in leaves while number of cobs plant -1 and flowering time were nonsignificantly affected by these treatments. These results suggested that k-carrageenan elicitor can be used as a potent plant protectant as well as growth promoting agent especially for chickpea plants. © 2010 King Saud University. Production and hosting by Elsevier B.V.


Khan K.M.,University of Karachi | Rahim F.,University of Karachi | Rahim F.,Hazara University | Shah S.A.A.,University Technology of MARA | And 5 more authors.
Journal of the Chemical Society of Pakistan | Year: 2014

An effective and eco-friendly method for the bis(indolyl)methanes synthesis has been developed. A successive methodology involving sodium iodate/sodium hydrogen sulfite catalyzed reaction of indole with many aldehydes gave the resultant bis-indol in good yield. This method offers synthetically inexpensive alternate to the previously developed procedures for the bis-indol synthesis. The use of a low-cost and straightforwardly accessible catalyst, improved yields and a simple reaction process are the salient features of the current method.


Farooq M.,University of Karachi | Akhtar Z.,University of Karachi | Saad S.M.,University of Karachi | Ali S.I.,University of Karachi | And 3 more authors.
Journal of the Chemical Society of Pakistan | Year: 2015

Summary: Disodium- 4,4 ′ - bis(1,3,5-triazinylamino)stilbene- 2,2 ′ - disulfonates 5a - 5d were synthesized by reacting 4,4′ -diaminostilbene-2- 2′ -disulfonic acid 1 with 2,4,6-trichloro-1,3,5-triazine 2 via the intermediacies of 3 and 4a-4d. The intermediate 3 was reacted with different amines at different temperature conditions to afford other intermediates 4a - 4d and finally the desired fluorescent brightener's 5a - 5d. The structures of the products were characterized by UV-visible, IR and H1 - NMR spectroscopy techniques. Elemental analyses were found to be in good agreement with the calculated values. These compounds were applied on cotton fiber as fluorescent brighteners and their physical properties were performed by wash-, chlorine-, and light-fastness. The whiteness of the fiber was measured by spectrophotometer. Fluorescent brightening agents UV-visible spectra Fastness properties Whiteness properties.


Khan K.M.,University of Karachi | Taha M.,University of Karachi | Rahim F.,University of Karachi | Ali M.,University of Karachi | And 3 more authors.
Letters in Organic Chemistry | Year: 2010

An improved method for the synthesis of disulfide in water by using periodic acid and sodium hydrogen sulfite was developed. © 2010 Bentham Science Publishers Ltd.


Khan K.M.,University of Karachi | Khan M.,University of Karachi | Khan M.,Abdul Wali Khan University Mardan | Saleem M.,University of Karachi | And 5 more authors.
Journal of the Chemical Society of Pakistan | Year: 2013

Carbonic anhydrase inhibitory activity of benzimidazole derivatives 1-24 has been evaluated. Compounds 22 (IC50 = 7.47 ± 0.39 μM), 21 (IC50 = 10.31 ± 0.11 μM), 20 (IC50 = 23.1 ± 1.78 μM), 12 (IC50 = 12.16 ± 0.10 μM), 11 (IC50 = 12. 81 ± 0.29 μM), and 15 (IC50 = 13.18 ± 0.37 μM) showed carbonic anhydrase inhibitory activity, and compared with the standard acetozolamide (IC50 = 0.31 ± 0.0006 μM). All the compounds were characterized by spectroscopic techniques.

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