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Kim T.K.,PCL Inc. | Jeong O.C.,Inje University
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 | Year: 2011

This paper presents the pneumatically-driven tensile stimulator for investigating the effect of mechanical strain on the intracellular calcium expression in MG-63 cells (human osteoblast-like bone cell line). An optically transparent micro tensile stimulator array consisting of the deformable diaphragms and the micro fluidic channels was fabricated with a polydimethylsiloxane (PDMS) and a glass substrate. A strain gradient generated by the three-dimensional dome-shape deformation of the circular diaphragm was applied to live cells seeded on the deformable diaphragm of the pneumatic stimulator. During operation, intracellular calcium responses were measured using a laser-scanning microscope. Our new finding from temporal responses of fluorescence intensities was that the period of measured calcium expressions of cells under different strain regions were matched well with the applied signal waveform of the external compressed air, and variations of the calcium expression might be also highly correlated with the magnitude of the strain. The release/uptake of intracellular calcium in the endoplasmic reticulum may be activated and more sensitive to applied strain. Copyright © (2011) by the Chemical and Biological Microsystems Society.


Ahn J.-Y.,Dongguk University | Lee S.,Lund University | Jo M.,PCL Inc. | Kang J.,Dongguk University | And 5 more authors.
Analytical Chemistry | Year: 2012

This paper reports for the first time the application of sol-gel microarrays for immobilizing nonsoluble small chemicals (Bisphenol-A; BPA). Also, known problems of sol-gel adhesion to conventional microtiter well plate substrates are circumvented by anchoring the sol-gel microspots to a porous silion surface so-called, PS-SG chips. We confirmed low molecular weight chemical immobilization inside a sol-gel network using fluorescein. BPA and the BPA specific aptamer were utilized as a model pair to verify the affinity specific interaction in the PS-SG selection system. The aptamer interacted specifically with BPA in the sol-gel spots, as shown in microarrays forming the letters "L", "U", "N", and "D". Moreover, the bound aptamer was released by heat, recovered, and verified by gel electrophoresis. The developed PS-SG chip platform will be used for screening aptamers against numerous small molecules such as toxins, metabolites, or pesticide residues. © 2012 American Chemical Society.


HOLLYWOOD--(BUSINESS WIRE)--The Advanced Imaging Society and The VR Society bestowed 28 honors for “distinguished achievement” at The Lumiere Awards, presented by AMD and Stereo D, on Monday night at Warner Bros. Studios in Hollywood. Filmmaker and VR Creator, Jon Favreau, was honored with the Society’s Harold Lloyd Award, presented last year to Marvel’s Victoria Alonso. Cher Wang of HTC Vive was honored with the Sir Charles Wheatstone Award for exemplifying exceptional forward movement in the VR Sciences. Her award was presented by AIS-VR Society President Jim Chabin and actress Maria Bello. “Billy Lynn’s Long Halftime Walk” (Sony Pictures Entertainment) was honored with a Lumiere statuette for Best 3D Live Action Feature. “Zootopia” (Walt Disney Studios) won for Best 3D Animation. “Dr. Strange” (Marvel Studios) won best 3D Stereography, Live Action and also 3D Scene of the Year for its journey of surreal astral projection. Google Earth VR was presented the Century Award by Ed Begley Jr. for VR in service of environmental enrichment. Two-time Academy-award winner, director Robert Stromberg presented Google with the award for Best VR Experience for Tilt Brush. “Ghostbusters VR Experience” was awarded the Lumiere for Best VR Film Experience (Sony Pictures Entertainment/The Void). And besides Best 3D Live Action Feature, Sony PlayStation and Sony PCL Inc./Vicom Inc. were also honored with Best VR Music Experience for “Joshua Bell VR” and Best UHD for “Miyako Island’s Therapeutic Beaches,” respectively. Best 3D Animated Stereography went to for “Kubo and the Two Strings” (Focus Features) and Best 3D Animated Short went to “Inner Workings” (Walt Disney Studios). Stereo D was honored with the Lumiere for Best Use of 2D to 3D Conversion for “Rogue One: A Star Wars Story.” “Zootopia” (Walt Disney Studios) won for Best 3D Animation Feature, “Amazing Mighty Micro Monsters 3D” (Atlantic Prods./Colossus Prods.) won Best 3D Documentary. The archeological experience, “Mysteries of China,” was honored with Best 3D Documentary Short (Giant Screen Films/Top Production/Expanded Eye Entertainment). For Best 3D Live Action Short, War of Gaul (Cow Prod) won the award. The Lumiere for Best VR Animation Experience was given to “Dear Angelica” (Oculus Story Studio) and Best VR Game went to “Job Simulator” by Owlchemy Labs. Best 360 Series for “Invisible” went to Doug Liman, 30 Ninjas, Condé Nast, Jaunt VR and Samsung, and “Nomads: Sea Gypsies” was honored for Best 360 Live Action (Felix & Paul Studios). “The Click Effect” (Annapurna Pictures/Here Be Dragons) and “Take Flight” (New York Times) won for Best VR Documentary and Best Journalism Experience, respectively. “ The 360 Tour of the Shinola Factory with Luke Wilson” won for Best VR Branded Experience (Reel FX). In the HDR categories, “Live By Night” (Warner Bros.) took home the Lumiere for Best HDR Live Action and “Finding Dory” (Pixar) won for Best HDR Animation.


Ahn J.-Y.,Cornell University | Ahn J.-Y.,Dongguk University | Jo M.,Dongguk University | Jo M.,PCL Inc. | And 3 more authors.
Oligonucleotides | Year: 2011

RNA and DNA aptamers that bind to target molecules with high specificity and affinity have been a focus of diagnostics and therapeutic research. These aptamers are obtained by SELEX often requiring many rounds of selection and amplification. Recently, we have shown the efficient binding and elution of RNA aptamers against target proteins using a microfluidic chip that incorporates 5 sol-gel binding droplets within which specific target proteins are imbedded. Here, we demonstrate that our microfluidic chip in a SELEX experiment greatly improved selection efficiency of RNA aptamers to TATA-binding protein, reducing the number of selection cycles needed to produce high affinity aptamers by about 80%. Many aptamers were identical or homologous to those isolated previously by conventional filter-binding SELEX. The microfluidic chip SELEX is readily scalable using a sol-gel microarray-based target multiplexing. Additionally, we show that sol-gel embedded protein arrays can be used as a high-throughput assay for quantifying binding affinities of aptamers. © 2011 Mary Ann Liebert, Inc.


Lee J.H.,Seoul National University of Science and Technology | Roh J.H.,Korea Electronics Technology Institute | Shin K.S.,Korea Electronics Technology Institute | Lee D.S.,Korea Electronics Technology Institute | And 4 more authors.
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 | Year: 2013

In this study, Si-NWs with the length of 200 jim, width of 200 nm, and height of 50 nm were fabricated using basic MEMS processes such as photolithography, RIE and KOH etching. They had high sensitivity and showed the FET characteristics due to high surface-to-volume ratio. Also, high affinity aptamers for prostate specific antigen (PSA) were immobilized on Si-NW using sol-gel method and then the real-time measurement of electrical signal of free-PSA and total-PSA which was guideline of prostate cancer was carried out according to PSA concentration. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved.


Chang C.I.,Sungkyunkwan University | Chang C.I.,Harvard University | Kim H.A.,PCL Inc. | Dua P.,Sungkyunkwan University | And 4 more authors.
Nucleic Acid Therapeutics | Year: 2011

Since the discovery of double-stranded (ds) RNA-mediated RNA interference (RNAi) phenomenon in Caenorhabditis elegans, specific gene silencing based upon RNAi mechanism has become a novel biomedical tool that has extended our understanding of cell biology and opened the door to an innovative class of therapeutic agents. To silence genes in mammalian cells, short dsRNA referred to as small interfering RNA (siRNA) is used as an RNAi trigger to avoid nonspecific interferon responses induced by long dsRNAs. An early structure-activity relationship study performed in Drosophila melanogaster embryonic extract suggested the existence of strict siRNA structural design rules to achieve optimal gene silencing. These rules include the presence of a 3′ overhang, a fixed duplex length, and structural symmetry, which defined the structure of a classical siRNA. However, several recent studies performed in mammalian cells have hinted that the gene silencing siRNA structure could be much more flexible than that originally proposed. Moreover, many of the nonclassical siRNA structural variants reported improved features over the classical siRNAs, including increased potency, reduced nonspecific responses, and enhanced cellular delivery. In this review, we summarize the recent progress in the development of gene silencing siRNA structural variants and discuss these in light of the flexibility of the RNAi machinery in mammalian cells. © 2011, Mary Ann Liebert, Inc.


PubMed | PCL Inc., Korea University, Dongguk University and Kangwon National University
Type: | Journal: BioMed research international | Year: 2015

Microarrays enable high-throughput screening (HTS) of disease-related molecules, including important signaling proteins/peptides and small molecules that are in low abundance. In this study, we developed a multiplex blood bank screening platform, referred to as the Hi3-1 assay, for simultaneous detection of human immunodeficiency virus 1/2 (HIV 1/2) and hepatitis C virus (HCV).The Hi3-1 assay was tested using four panels (Panel 1, n = 4,581 patient samples; Panel 2, n = 15 seroconversion samples; Panel 3, n = 4 performance samples; and Panel 4, n = 251 purchased positive control samples), and the results were collected by the Department of Laboratory Medicine, Korea University Medical College, Republic of Korea. The present study compares the sensitivity of the multiplex detection platform for both HIV and HCV using a sol-gel based microarray, which was based on a reference test (Architect HIV Ag/Ab Combo and Architect anti-HCV assays), in Korean patients.The sensitivity of the multiplex detection platform for both HIV and HCV was 100%, and the specificity was 99.96% for HIV and 99.76% for HCV, which is equivalent to that of the reference test.We have successfully applied a novel screening technology to multiplex HIV and HCV diagnoses in a blood bank screening test.


A method of preparing a protein chip by gelation of a sol composition. In the method, a mixture of specific silicate monomers, such as SolB1, SolB2 and SolB3, SolBH, and a mixture of SolBS, distilled water and a detector protein are mixed sequentially, so that the gelation rate of the sol-composition can be delayed, thus inducing the stable gelation of the composition. Also, the biochip can be fabricated in a simple and easy manner by dispensing the sol composition by hand using an arrayer or a tool such as a pipette. In addition, a uniform biochip can be prepared by dispensing the sol composition, solution I (SolBH) and solution II (a mixture of buffer, SolBS, distilled water and a detector protein) sequentially onto a substrate without needing a conventional pretreatment process such as mixing.


The present invention relates to a multiplex microfluidic device for selection of nucleic acid aptamers and a method for high-throughput selection of nucleic acid aptamers using the same, and more particularly to a multiplex microfluidic device (SELEX lap-on-a-chip) that uses an improved multiplex platform in place of the development of an aptamer for a single target and to a method for high throughput selection of aptamers using the same together with high-throughput sequencing. A multiplex microfluidic device according to the present invention can simultaneously detect aptamers for a plurality of targets, and it can greatly increase the screening throughput and greatly shorten the process time compared to conventional multiplex techniques. Particularly, when a process for selecting aptamers is performed using the device of the invention together with a high-throughput sequencing method, the number of target binding/elution/amplification rounds can be greatly reduced, and the process can be performed in an automated manner. Thus, the device of the invention is highly useful.


The present invention relates to a method of preparing a protein chip by gelation of a sol composition. In the method, a mixture of specific silicate monomers, such as SolB1, SolB2 and SolB3, SolBH, and a mixture of SolBS, distilled water and a detector protein are mixed sequentially, so that the gelation rate of the sol composition can be delayed, thus inducing the stable gelation of the composition. Also, the biochip can be fabricated in a simple and easy manner by dispensing the sol composition by hand using an arrayer or a tool such as a pipette. In addition, a uniform biochip can be prepared by dispensing the sol composition, solution I (SolBH) and solution II (a mixture of buffer, SolBS, distilled water and a detector protein) sequentially onto a substrate without needing a conventional pretreatment process such as mixing.

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