PCB Unit of Experimental Toxicology and Ecotoxicology UTOX PCB

Barcelona, Spain

PCB Unit of Experimental Toxicology and Ecotoxicology UTOX PCB

Barcelona, Spain
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De Marzi L.,University of L'Aquila | Monaco A.,University of L'Aquila | De Lapuente J.,PCB Unit of Experimental Toxicology and Ecotoxicology UTOX PCB | Ramos D.,PCB Unit of Experimental Toxicology and Ecotoxicology UTOX PCB | And 4 more authors.
International Journal of Molecular Sciences | Year: 2013

Owing to their radical scavenging and UV-filtering properties, ceria nanoparticles (CeO2-NPs) are currently used for various applications, including as catalysts in diesel particulate filters. Because of their ability to filter UV light, CeO2-NPs have garnered significant interest in the medical field and, consequently, are poised for use in various applications. The aim of this work was to investigate the effects of short-term (24 h) and long-term (10 days) CeO2-NP exposure to A549, CaCo2 and HepG2 cell lines. Cytotoxicity assays tested CeO2-NPs over a concentration range of 0.5 μg/mL to 5000 μg/mL, whereas genotoxicity assays tested CeO2-NPs over a concentration range of 0.5 μg/mL to 5000 μg/mL. In vitro assays showed almost no short-term exposure toxicity on any of the tested cell lines. Conversely, long-term CeO2-NP exposure proved toxic for all tested cell lines. NP genotoxicity was detectable even at 24-h exposure. HepG2 was the most sensitive cell line overall; however, the A549 line was most sensitive to the lowest concentration tested. Moreover, the results confirmed the ceria nanoparticles' capacity to protect cells when they are exposed to well-known oxidants such as H2O2. A Comet assay was performed in the presence of both H2O2 and CeO2-NPs. When hydrogen peroxide was maintained at 25 μM, NPs at 0.5 μg/mL, 50 μg/mL, and 500 μg/mL protected the cells from oxidative damage. Thus, the NPs prevented H2O2-induced genotoxic damage. © 2013 by the authors; licensee MDPI, Basel, Switzerland.


Perrier M.,Charles Gerhardt Institute | Gallud A.,Montpellier University | Ayadi A.,Montpellier University | Kennouche S.,Montpellier University | And 11 more authors.
Nanoscale | Year: 2015

Cyano-bridged Gd3+/[Fe(CN)6]3- coordination polymer nanoparticles of 3-4 nm stabilized with d-mannitol presenting a high r1 relaxivity value of 11.4 mM-1 s-1 were investigated in vivo as contrast agents (CA) for Magnetic Resonance Imaging (MRI). They allow an increase of the MR image contrast and can act as an efficient intravascular T1 CA with a relatively long blood-circulation lifetime (60 min) without specific toxicity. This journal is © The Royal Society of Chemistry.


Perrier M.,Charles Gerhardt Institute | Busson M.,Montpellier University | Massasso G.,Charles Gerhardt Institute | Long J.,Charles Gerhardt Institute | And 9 more authors.
Nanoscale | Year: 2014

Prussian blue (PB) and its analogues on the nanometric scale are exciting nano-objects that combine the advantages of molecular-based materials and nanochemistry. Herein, we demonstrate that ultra-small PB nanoparticles of 2-3 nm can be easily labelled with radioactive 201Tl+ to obtain new nanoprobes as radiotracers for 201-thallium-based imaging. This journal is © The Royal Society of Chemistry.

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