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Sadeghy B.,Payamnoor University of Kerman | Rohani M.,Ministry of Agriculture | Aghasi N.,Ministry of Agriculture
Archives of Phytopathology and Plant Protection | Year: 2014

In this research, distribution of Phytophthora species were determined in Kerman Province (Bam, Shahdad and Arzuiyeh) during 2004-2007. The Phytophthora species were isolated from infected root, crown and soil. Root and crown pieces were washed and cultured on a CMA-PARPH medium. The isolation from infected soil was performed by bating method using citrus leaves. It was identified by morphological and several physiological characteristics. Distribution of species was determined by recording the number of isolates recovered from samples from each city. In this study, from 220 soil samples collected from 52 citrus orchards, 80 isolates of Phytophthora were recovered. Among of all isolates of Phytophthora, P. parasitica and P. citrophthora were the most important species of causal agent of gummosis on Citrus sp. Distribution of P. citrophthora was highest in Arzuiyeh and lowest in Bam and Shahdad cities, while distribution of P. parasitica was highest in Bam and Shahdad cities. The majority of soil samples contained only P. parasitica, but a few of percentage samples containing P. citrophthora alone and both of fungi in cites samples. © 2013 © 2013 Taylor & Francis. Source


Sadeghy B.,Payamnoor University of Kerman | Javan-Nikkhah M.,University of Tehran | Nayebyazdi N.,University of Zabol
Archives of Phytopathology and Plant Protection | Year: 2014

In the identification and investigation virulence of Phytophthora species, the causal agent of root rot and gummosis citrus was investigated in Kerman from 2004 to 2007. Twenty-seven of 70 isolates were Phytophthora citrophthora, and 33 isolates were identified as Phytophthora parasitica. These species were isolated from soil, crown and root trees. Also, 10 isolates were identified as Phytophthora cryptogea. This species was isolated from soils of around declining citrus trees in Bam region using citrus leaf discs. The subset of these isolates was tested for pathogenicity on Citrus aurantium. Results of canker length and weight of fresh root indicated a variation in virulence among Phytophthora species. P. parasitica sh-1 from Shahdad and P. citrophthora o-25 from Orzuyeh were the most virulent on fresh weight of root and Canker length on crown. The P. parasitica isolates from three regions significantly produced the most virulent on roots relative to other isolates and P. citrophthora isolates significantly produced the most virulent on crown, while P. cryptogea b-5 from Bam was the least virulent. P. parasitica sh-1 created the most reduction in weight of fresh roots. Furthermore, P. citrophthora o-25 significantly caused the most virulent relative to P. citrophthora b-57 and b-5 isolates. All P. parasitica and P. citrophthora isolates produced root rot and crown canker but P. cryptogea no pathogenic on crown but produced weak root rot on citrus seedlings. The low virulent of P. cryptogea b-5 suggested that this pathogen is not a serious threat to C. aurantium. © 2013 © 2013 Taylor & Francis. Source


Sadeghy B.,Payamnoor University of Kerman | Hatamy N.,Payamnoor University of Kerman
Archives of Phytopathology and Plant Protection | Year: 2013

In this study, biological activity of 70 Streptomyces isolates was evaluated against Verticillium dahliae and Fusarium sambucinum by dual culture techniques. Among all isolates which were screened, isolate C-1 showed high level of activity on agar disc and well diffusion methods against V. dahliae and F. sambucinum. Active time was monitored in shaked culture; Streptomyces isolate C-1 activities reached maximum after 10 and 4 days against V. dahliae and F. sambucinum, respectively. Different temperature and pH effects were also evaluated on mycelial growth of Streptomyces sp. isolate C-1. Evaluation of relative polarity of the active and pH effects on principle present in the crude showed that it has polar nature and was rather alkaliphilic. Also, this isolate has high salt tolerance and observed to have fungicidal activity against V. dahliae and F. sambucinum. © 2013 © 2013 Taylor & Francis. Source


Sadeghy B.,Payamnoor University of Kerman | Hatamy N.,Payamnoor University of Kerman
Archives of Phytopathology and Plant Protection | Year: 2014

Seventy Streptomyces isolates were obtained from the Rhizosphere and soil of greenhouse and their antagonistic activities against Fusarium subglutinans in dual culture test was studied. Two isolates had antagonistic activities against F. subglutinans. These isolates were tested for determining the antagonistic mechanism effects of extracellular metabolites (such as amylase, protease and lipase). The results exhibited that two isolates of Streptomyces sp. C-11 and C-26 have the ability to produce enzymes, such as amylase, protease and lipase, in vitro. © 2013 © 2013 Taylor & Francis. Source


Sadeghy B.,Payamnoor University of Kerman | Hatami N.,Payamnoor University of Kerman
Archives of Phytopathology and Plant Protection | Year: 2014

About 70 Streptomyces species, isolated from soils of greenhouses and citrus orchards were evaluated for their antagonistic activity against Verticillium dahliae, Fusarium subglutinans, Fusarium sambucinum, Phoma glomerata and Nattrassia mangiferae. Preliminary screening for antimicrobial activity was determined by dual culture method. The soils of Kerman are rich sources of micro-organisms with potent biological activities, and screening programmes are to be conducted to reveal the presence of active Actinomycetes isolates against phytopathogenic fungi. © 2013 Taylor & Francis. Source

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