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Colagar A.H.,University of Mazandaran | Yusefi M.,Payam e Noor University of Esfahan | Zarei M.,University of Mazandaran | Yousefzadeh H.,Tarbiat Modares University
Polish Journal of Ecology | Year: 2013

Genetic variability and differentiation of six populations of Tilia rubra from Hyrcanian forests of the north of Iran were analyzed using random amplified polymorphic DNA (RAPD) markers. Seven primers pairs used to study six populations, in six localities located in at different altitude (250-2500 m a.s.l) but within the distance 50 km, provided 70 reliable bands, of which 29 (41.4%) were polymorphic. The average population diversity using the Shannon's index (I) was 0.22 and ranged from 0.22 for Bandbon population to 0.08 for the Salahedinkola population. The mean heterozygosity varied from 0.013 to 0.06 within the populations of T. rubra. The AMOVA analysis showed a significant genetic differentiation within populations and 97% of the total variation occurred within populations and only 3% occurred among populations. Cluster analysis using the UPGMA method grouped all individuals into four clusters. The results did not correspond with the populations origin. The low inter-population differentiation and the lack of significant correlation between geographic and genetic distances suggest intensive gene flow among populations of Tilia rubra. Source

Barfi A.,Payam e Noor University of Esfahan | Nazem H.,Payam e Noor University of Esfahan | Saeidi I.,Iranian National Standards Organization | Peyrovi M.,University of Mazandaran | And 2 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2016

In the present study, an efficient and environmental friendly method (called in-syringe reversed dispersive liquid-liquid microextraction (IS-R-DLLME)) was developed to extract three important components (i.e. para-anisaldehyde, trans-anethole and its isomer estragole) simultaneously in different plant extracts (basil, fennel and tarragon), human plasma and urine samples prior their determination using high-performance liquid chromatography. The importance of choosing these plant extracts as samples is emanating from the dual roles of their bioactive compounds (trans-anethole and estragole), which can alter positively or negatively different cellular processes, and necessity to a simple and efficient method for extraction and sensitive determination of these compounds in the mentioned samples. Under the optimum conditions (including extraction solvent: 120μL of n-octanol; dispersive solvent: 600μL of acetone; collecting solvent: 1000μL of acetone, sample pH 3; with no salt), limits of detection (LODs), linear dynamic ranges (LDRs) and recoveries (R) were 79-81ngmL-1, 0.26-6.9μgmL-1 and 94.1-99.9%, respectively. The obtained results showed that the IS-R-DLLME was a simple, fast and sensitive method with low level consumption of extraction solvent which provides high recovery under the optimum conditions. The present method was applied to investigate the absorption amounts of the mentioned analytes through the determination of the analytes before (in the plant extracts) and after (in the human plasma and urine samples) the consumption which can determine the toxicity levels of the analytes (on the basis of their dosages) in the extracts. © 2016 Elsevier B.V. Source

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