Paul Laurence Dunbar High School

Lexington, KY, United States

Paul Laurence Dunbar High School

Lexington, KY, United States
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News Article | May 11, 2017

Comcast, joined by Dr. Carol Williamson, Deputy Superintendent, Office of Teaching and Learning at the Maryland State Department; Maryland House Speaker, Michael E. Busch; and other local elected officials and school administrators, recognized the students at a special event held Wednesday, May 10, at Maryland Hall for the Creative Arts in Annapolis. One hundred and nine recipients of the 2017 Maryland Leaders and Achievers® scholarships received $1,000. Cienna Bell, a senior at Bowie High School in was awarded a $10,000 Comcast Founders Scholarship – instituted in honor of Ralph J. Roberts, Founder and Chairman Emeritus of Comcast Corporation – for a total of $119,000 awarded this year to Maryland high school students. "Our Leaders and Achievers Scholarship winners are committed to academic excellence and community service," said Mary McLaughlin, Senior Vice President of Comcast's Beltway Region. "We are honored to recognize their achievements, and are excited to support them as they continue their educational journeys." The Comcast Leaders and Achievers Scholarship Program provides scholarships to students who strive to achieve their full potential, who are catalysts for positive change in their communities, who are involved in their schools, and who serve as models for their fellow students. The philosophy behind the program is to give young people every opportunity to prepare for the future and to engage them in their communities. The program also demonstrates the importance of civic involvement, and the value placed on civic involvement by the business community. Since 2009, Comcast has awarded nearly $825,000 in Leaders & Achievers Scholarships to more than 750 students in Maryland. This year, the program will award more than $2 million in scholarships to more than 2,000 students across the country to help them pursue higher education. Visit here to learn more. Olivia Bailey of Chesapeake High School in Pasadena Savannah Edmonds of Archbishop Spalding High School in Severn Allison Gingerich of Broadneck High School in Annapolis Scott Howarth of Arundel High School in Gambrills Cameron Laque of North County High School in Glen Burnie Hiro Motta of Glen Burnie High School Ian Robertson of Annapolis High School Kealsey Sajol of Old Mill High School in Millersville Tabitha Blackston of Merganthaler Vocational-Technical High School Jenae Burrell of Reginald F. Lewis High School Kayla Carr of Paul Laurence Dunbar High School Michael Cheng of Gilman School Kayla Demeritte of Coppin Academy High School Anthony Evans of Edmondson-Westside High School Lauren Fink of Baltimore Polytechnic Institute Tselani Huntley of Baltimore Renaissance Academy Sheila Mcmorris of Vivien T. Thomas Medical Arts Academy Ifetayo Jabari-Kitwala of Baltimore School for the Arts David Medlin of Forest Park Senior High Jamie Mintz of Benjamin Franklin High School at Masonville Cove Jonah Myers of Greater Grace Christian Academy Patrice Newsome of National Academy Foundation High School Morgan Pettus of Frederick Douglass High School Shemar Shields of Augusta Fells Savage Institute of Visual Arts High School Abeni Teal of Mercy High School Bria Thomas of Cristo Rey Jesuit High School Yarnee Whitaker of The REACH! Partnership School Vasiliki Argeroplos of Garrison Forest School in Owings Mills Mya Buschman of Maryvale Preparatory School in Lutherville Annalea Cascio of Parkville High School Natassia Celnik of St. Timothy's School in Stevenson Trinadee Coates of Randallstown High School Patrick Fuller of Perry Hall High School Benjamin Glaser of Western School of Technology Moshe Gordon of Israel Henry Beren High School in Pikesville Abigail Hauer of Eastern Tech High School Rachel Krakat of Concordia Preparatory School in Towson Emily Lane of Loch Raven High School Satia Longe of Patapsco High School Alyse Messafi of Beth Tfiloh Dahan Community School Annalise Michaelson of McDonogh School in Owings Mills Michael Millin of Jemicy School in Owings Mills Rebecca Olusola of New Town High School in Owings Mills Justin Perkins of Owings Mills High School Jamie Van Wyk of Pikesville High SchoolCailyn Walter of Notre Dame Preparatory School in Towson Kristen Almuete of Calvert High School in Prince Frederick Armani Claggett of Patuxent High School in Lusby Adam Fairchild of Manchester Valley High School Yuchen Luo of Carroll Christian Schools in Westminster Madison Storm of Westminster Senior High School De'John Broadwater of Thomas Stone High School in Waldorf Taylor Covington of Maurice J. McDonough High School in Pomfret Lindsey Johnson of North Point High School for Science, Technology & Industry in Waldorf Niki Kelley of St. Charles High School in Waldorf Andrei Maderazo of Westlake High School in Waldorf Zachary McDonough of Southern Maryland Christian Academy in White Plains Riley Weinberg of La Plata High School Brooke Bennett of Frederick County Career & Technology Center Catharine Dietrich of Linganore High School Gabriella Farrell of Frederick High School Miriam Tirado of Governor Thomas Johnson High School Ryan Brownfield of C. Milton Wright High School in Bel Air Sara Decker of Aberdeen High School Bethany Ingram of Bel Air High School Marissa Smith of Havre De Grace High School Marcus Anderson of Hammond High School in Columbia Samuel Chan of La Salle Homeschool Academy Mikayla Dixon of Wilde Lake High School in Columbia Vanneshja Hill-Edwards of Atholton High School in Columbia Xiao Kuang of Long Reach High School in Columbia Claire Lee of Centennial High School in Ellicott City Yizuan Liu of Marriotts Ridge High School in Marriottsville Aaron Luther of Mt. Hebron High School in Ellicott City Cire Nicholson of Oakland Mills High School in Columbia Jennifer Zhang of River Hill High School in Clarksville Jimmy Allah-Mensah of Northwest High School in Germantown Nicole Averinos of Quince Orchard High School in Gaithersburg Boaz Bamiro of Gaithersburg High School Autumn Barber of Richard Montgomery High School in Rockville Rudaelle Elien of Montgomery Blair High School in Silver Spring Anurudh Ganesan of Clarksburg High School Liam Gil of Wheaton High School in Silver Spring Michael Katski of Paint Branch High School in Burtonsville Nina Lao of Seneca Valley High School in Germantown Flosha Diliena Liyana Saran Arachchige Don of Col. Zadok Magruder High School in Rockville Rose Makor of Sherwood High School in Sandy Spring Taylor McDaniels of Our Lady of Good Counsel High School in Olney Wendy Medrano of Don Bosco Cristo Rey High School in Takoma Park Matthew Millstein of James Hubert Blake High School in Silver Spring Natalie Mogrovejo of Thomas Edison High School of Technology in Silver Spring Elizabeth Olaiya of Northwood High School in Silver Spring Jacob Rains of Bethesda-Chevy Chase High School in Bethesda Brandon Rodriguez of Watkins Mill High School in Gaithersburg Akehnji AchiriMofor of Laurel High School Cieanna Bell of Bowie High School Juwan Blocker of Parkdale High School in Riverdale Trevante Brown of Oxon Hill High School Gexi Chavez Bonilla of Eleanor Roosevelt High School in Greenbelt Taylor Custis of Grace Brethren Christian School in Clinton Erin Farley of Bishop Mcnamara High School in Forestville Skylar Johnson of St. Vincent Pallotti High School in Laurel Bedelina Miller of New Hope Academy in Landover Hills Samuel Morgan of From the Heart Christian School in Suitland Andrea Nickens of Crossland High School in Temple Hills Maya Sullivan of Elizebeth Seton High School in Bladensburg Tatyanna Sutton of Riverdale Baptist School in Upper Marlboro Carlotta Tyler of Progressive Learning Academy Jason Hentschel of James M. Bennett Senior High School in Salisbury Onya Turner of Wicomico High School in Salisbury About Comcast Corporation Comcast Corporation (Nasdaq: CMCSA) is a global media and technology company with two primary businesses, Comcast Cable and NBCUniversal. Comcast Cable is one of the nation's largest video, high-speed internet, and phone providers to residential customers under the XFINITY brand, and also provides these services to businesses. It also provides wireless and security and automation services to residential customers under the XFINITY brand. NBCUniversal operates news, entertainment and sports cable networks, the NBC and Telemundo broadcast networks, television production operations, television station groups, Universal Pictures and Universal Parks and Resorts. Visit for more information. About the Comcast Foundation The Comcast Foundation was founded by Comcast Corporation in June 1999 to provide charitable support to qualified non-profit organizations. The Foundation primarily invests in programs intended to have a positive, sustainable impact on their communities. The Foundation has three community investment priorities—promoting service, expanding digital literacy, and building tomorrow's leaders. Since its inception, the Comcast Foundation has donated nearly $200 million to organizations in the communities nationwide that Comcast serves. More information about the Foundation and its programs is available at To view the original version on PR Newswire, visit:

Wang H.,University of Kentucky | Horbinski C.,Northwestern University | Wu H.,Emory University | Liu Y.,University of Kentucky | And 5 more authors.
Nucleic Acids Research | Year: 2016

The advanced medium-throughput NanoString nCounter technology has been increasingly used for mRNA or miRNA differential expression (DE) studies due to its advantages including direct measurement of molecule expression levels without amplification, digital readout and superior applicability to formalin fixed paraffin embedded samples. However, the analysis of nCounter data is hampered because most methods developed are based on t-tests, which do not fit the count data generated by the NanoString nCounter system. Furthermore, data normalization procedures of current methods are either not suitable for counts or not specific for NanoString nCounter data. We develop a novel DE detection method based on NanoString nCounter data. The method, named NanoStringDiff, considers a generalized linear model of the negative binomial family to characterize count data and allows for multifactor design. Data normalization is incorporated in the model framework through data normalization parameters, which are estimated from positive controls, negative controls and housekeeping genes embedded in the nCounter system. We propose an empirical Bayes shrinkage approach to estimate the dispersion parameter in the model and a likelihood ratio test to identify differentially expressed genes. Simulations and real data analysis demonstrate that the proposed method performs better than existing methods. © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

Kamineni S.,University of Kentucky | Norgren C.R.,University of Kentucky | Davidson E.M.,University of Kentucky | Kamineni E.P.,Paul Laurence Dunbar High School | Deane A.S.,Indiana University
World Journal of Orthopaedics | Year: 2017

AIM To provide a "patient-normalized" parameter in the proximal forearm. METHODS Sixty-three cadaveric upper extremities from thirty-five cadavers were studied. A muscle splitting approach was utilized to locate the posterior interosseous nerve (PIN) at the point where it emerges from beneath the supinator. The supinator was carefully incised to expose the midpoint length of the nerve as it passes into the forearm while preserving the associated fascial connections, thereby preserving the relationship of the nerve with the muscle. We measured the transepicondylar distance (TED), PIN distance in the forearm's neutral rotation position, pronation position, supination position, and the nerve width. Two individuals performed measurements using a digital caliper with inter-observer and intraobserver blinding. The results were analyzed with the Wilcoxon-Mann-Whitney test for paired samples. RESULTS In pronation, the PIN was within two confidence intervals of 1.0 TED in 95% of cases (range 0.7-1.3 TED); in neutral, within two confidence intervals of 0.84 TED in 95% of cases (range 0.5-1.1 TED); in supination, within two confidence intervals of 0.72 TED in 95% of cases (range 0.5-0.9 TED). The mean PIN distance from the lateral epicondyle was 100% of TED in a pronated forearm, 84% in neutral, and 72% in supination. Predictive accuracy was highest in supination; in all cases the majority of specimens (90.47%-95.23%) are within 2 cm of the forearm position-specific percentage of TED. When comparing right to left sides for TEDs with the signed Wilcoxon-Mann-Whitney test for paired samples as well as a significance test (with normal distribution), the P -value was 0.0357 (significance - 0.05) indicating a significant difference between the two sides. CONCLUSION This "patient normalized" parameter localizes the PIN crossing a line drawn between the lateral epicondyle and the radial styloid. Accurate PIN localization will aid in diagnosis, injections, and surgical approaches. © The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.

Ortega J.,University of Kentucky | Li J.Y.,Paul Laurence Dunbar High School | Li J.Y.,Yale University | Lee S.,University of Kentucky | And 5 more authors.
Proceedings of the National Academy of Sciences of the United States of America | Year: 2015

Proliferating or cell nuclear antigen (PCNA) plays essential roles in eukaryotic cells during DNA replication, DNA mismatch repair (MMR), and other events at the replication fork. Earlier studies show that PCNA is regulated by posttranslational modifications, including phosphorylation of tyrosine 211 (Y211) by the epidermal growth factor receptor (EGFR). However, the functional significance of Y211-phosphorylated PCNA remains unknown. Here, we show that PCNA phosphorylation by EGFR alters its interaction with mismatch-recognition proteins MutSα and MutSβ and interferes with PCNA-dependent activation of MutLα endonuclease, thereby inhibiting MMR at the initiation step. Evidence is also provided that Y211-phosphorylated PCNA induces nucleotide misincorporation during DNA synthesis. These findings reveal a novel mechanism by which Y211-phosphorylated PCNA promotes cancer development and progression via facilitating error-prone DNA replication and suppressing the MMR function. © 2015, National Academy of Sciences. All rights reserved.

Li X.,University of Kentucky | Stevens P.D.,University of Kentucky | Liu J.,University of Kentucky | Yang H.,Taizhou Hospital | And 7 more authors.
Gastroenterology | Year: 2014

Background & Aims Hyperactivation of the RAS-RAF signaling pathway in colorectal tumors is associated with metastasis and poor outcomes of patients. Little is known about how RAS-RAF signaling is turned off once activated. We investigated how the pH domain and leucine-rich repeat protein phosphatases (PHLPPs) control RAS-RAF signaling and colorectal cancer (CRC) development. Methods We used co-immunoprecipitation assays to identify substrates of PHLPP1 and PHLPP2. We studied phosphorylation of RAF1 in CRC cells that express exogenous PHLPP1 or PHLPP2, or lentiviral-based small hairpin RNAs against their transcripts; we measured effects on cell motility, migration, and invasion in vitro. Tumor progression and survival were analyzed in Phlpp1-/- ApcMin and ApcMin/Phlpp1-/- mice. Microarray datasets of colorectal tumor and nontumor tissues were analyzed for PHLPP gene expression. Results PHLPP1 and 2 were found to dephosphorylate RAF1 at S338, inhibiting its kinase activity in vitro and in CRC cells. In cells, knockdown of PHLPP1 or PHLPP2 increased the amplitude and duration of RAF-MEK-ERK signaling downstream of epidermal growth factor receptor and KRAS, whereas overexpression had the opposite effect. In addition, knockdown of PHLPP1 or PHLPP2 caused CRC cells to express markers of the epithelial-mesenchymal transition, and increased cell migration and invasion. ApcMin/Phlpp1-/- mice had decreased survival and developed larger intestinal and colon tumors compared to ApcMin mice. Whereas ApcMin mice developed mostly low-grade adenomas, 20% of the tumors that developed in ApcMin/ Phlpp1-/- mice were invasive adenocarcinomas. Normal villi and adenomas of ApcMin/Phlpp1-/- mice had significantly fewer apoptotic cells than ApcMin mice. Human CRC patient microarray data revealed that the expression of PHLPP1 or PHLPP2 is positively correlated with CDH1. Conclusions PHLPP1 and PHLPP2 dephosphorylate RAF1 to reduce its signaling, increase the invasive and migratory activities of CRC cells, and activate the epithelial-mesenchymal transition. In ApcMin mice, loss of PHLPP1 promotes tumor progression.

PubMed | Tsinghua University, University of Kentucky and Paul Laurence Dunbar High School
Type: Journal Article | Journal: Proceedings of the National Academy of Sciences of the United States of America | Year: 2015

Proliferating cell nuclear antigen (PCNA) plays essential roles in eukaryotic cells during DNA replication, DNA mismatch repair (MMR), and other events at the replication fork. Earlier studies show that PCNA is regulated by posttranslational modifications, including phosphorylation of tyrosine 211 (Y211) by the epidermal growth factor receptor (EGFR). However, the functional significance of Y211-phosphorylated PCNA remains unknown. Here, we show that PCNA phosphorylation by EGFR alters its interaction with mismatch-recognition proteins MutS and MutS and interferes with PCNA-dependent activation of MutL endonuclease, thereby inhibiting MMR at the initiation step. Evidence is also provided that Y211-phosphorylated PCNA induces nucleotide misincorporation during DNA synthesis. These findings reveal a novel mechanism by which Y211-phosphorylated PCNA promotes cancer development and progression via facilitating error-prone DNA replication and suppressing the MMR function.

Sun W.,University of Kentucky | Yang K.,Paul Laurence Dunbar High School | Yang F.,University of Kentucky
Journal of Polymer Research | Year: 2015

A simple template consisting of two contacting metallic wires was developed to construct surface structures on PMMA films. Using this template, surface structures including arcs and straight stripes were formed on the surface of PMMA films via the evaporation of toluene droplets. The effect of the angle between two wires and the film thickness on the geometric characteristics of the structures formed was studied. For the same film thickness, the amplitude is approximately a linear, increasing function of the wavelength with approximately the same slope, independent of the angle between the wires. The wavelength of the surface structures increases with the film thickness for the same distance to the wire and the same angle between the wires. This technique provides a viable technique to form surface structures over a large surface area of polymer films for various applications. © 2015, Springer Science+Business Media Dordrecht.

Fang J.,Zhejiang University | Yu L.,University of Kentucky | Wu M.,Paul Laurence Dunbar High School | Wei Y.,University of Kentucky
Journal of Biomolecular Structure and Dynamics | Year: 2013

The resistance-nodulation-cell division family multidrug transporter Acriflavine resistance protein B (AcrB) from Eschericha coli is an obligate homotrimer. Approximately, 45% of the inter-subunit interface is contributed by a protruding loop (also referred to as the thumb) and its corresponding binding tunnel in the neighboring subunit. In an earlier study, we have demonstrated that a single Pro to Gly mutation in the loop drastically destabilized AcrB trimer and reduced its substrate efflux activity. To further dissect the role of the loop during AcrB trimerization, we performed Ala scanning of the loop and examined the effect of each mutation on protein activity. We found that not all conserved residues are important for AcrB function and likewise not all critical residues are conserved. In addition, we replaced the loop of AcrB with the loop of MexB, which is a highly conserved homolog of AcrB. The resultant chimeric protein remained partly active. Structural characterization of the chimeric protein indicated that it was well folded and existed as a mixture of monomer and trimer. Our results indicate that the loop to tunnel interaction, while critical to trimerization and efflux function, is in general rather flexible and tolerant to mutations. In addition, all mutations in the loop that resulted in reduced function clustered closely, suggesting that this may be the site of inter-subunit recognition during trimerization and/or a locking zone to stabilize the inter-subunit interaction during trimerization. Copyright © 2012 Taylor & Francis.

Knecht L.D.,University of Miami | Ali N.,Paul Laurence Dunbar High School | Wei Y.,University of Kentucky | Hilt J.Z.,University of Kentucky | Daunert S.,University of Miami
ACS Nano | Year: 2012

Nanomaterials have found numerous applications as tunable, remotely controlled platforms for drug delivery, hyperthermia cancer treatment, and various other biomedical applications. The basis for the interest lies in their unique properties achieved at the nanoscale that can be accessed via remote stimuli. These properties could then be exploited to simultaneously activate secondary systems that are not remotely actuatable. In this work, iron oxide nanoparticles are encapsulated in a bisacrylamide cross-linked polyacrylamide hydrogel network along with a model dehalogenase enzyme, L-2-HAD ST. This thermophilic enzyme is activated at elevated temperatures and has been shown to have optimal activity at 70 °C. By exposing the Fe 3O 4 nanoparticles to a remote stimulus, an alternating magnetic field (AMF), enhanced system heating can be achieved, thus remotely activating the enzyme. The internal heating of the nanocomposite hydrogel network in the AMF results in a 2-fold increase in enzymatic activity as compared to the same hydrogel heated externally in a water bath, suggesting that the internal heating of the nanoparticles is more efficient than the diffusion-limited heating of the water bath. This system may prove useful for remote actuation of biomedical and environmentally relevant enzymes and find applications in a variety of fields. © 2012 American Chemical Society.

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