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Brisbane, Australia

Kamolvit W.,University of Queensland | Derrington P.,Pathology Queensland | Paterson D.L.,University of Queensland | Sidjabat H.E.,University of Queensland
Journal of Clinical Microbiology | Year: 2015

An IMP-4-producing Acinetobacter pittii strain coproducing oxacillinases was isolated from a leg wound of a 67-year-old female patient. Identification to the species level by rpoB and gyrB sequencing and multiplex-PCR-based analysis revealed that the isolate was A. pittii. Whole-genome sequencing of this A. pittii isolate determined the presence of blaOXA-96, blaCARB-2, and a novel blaOXA-421 gene. The position of this novel blaOXA-421 gene was similar to that of blaOXA-51 in A. baumannii, downstream of the phosphinothricin N-acetyltransferase gene and upstream of fxsA in the chromosome. This A. pittii isolate was found to belong to sequence type 119 (ST119). Here, we report the first isolation of IMP-4-producing A. pittii ST119 with a novel blaOXA-421 gene from a patient in Australia and characterize its draft genome. © 2015, American Society for Microbiology. All Rights Reserved. Source


Cooke R.A.,Pathology Queensland
Virchows Archiv | Year: 2010

The technique of making moulages (wax models) for the teaching of anatomy was introduced in Florence from the late 1600s. Studying the moulages was a much more pleasant way of learning anatomy than the alternative of dissecting dead bodies, when the tissues were undergoing postmortem decay. The technique spread to other medical schools in Europe as well as to England, Russia, North and South America, and Japan. The introduction of photography in the late 1800s made the moulageurs redundant. However, all of these countries now have historical museums that exhibit the specimens that remain from this period. A few moulageurs did continue to make specimens, mainly of dermatological conditions, right into the late 1900s. In 2005, the University of Zurich opened a new museum to showcase some such moulages made by local artists. © 2010 Springer-Verlag. Source


Dimeski G.,Pathology Queensland | Badrick T.,Sullivan Nicolaides Pathology | John A.S.,ARC Consulting
Clinica Chimica Acta | Year: 2010

Ion Selective Electrodes (ISEs) are used to measure some of the most critical analytes on clinical laboratory and point-of-care analysers. These analytes which include Na+, K+, Cl-, Ca2+, Mg2+ and Li+ are used for rapid patient care decisions. Although the electrodes are very selective, they are not free of interferences. It is important for laboratories to have an understanding of the type and extent of interferences in order to avoid incorrect clinical decisions and treatment. © 2009 Elsevier B.V. All rights reserved. Source


Pretorius C.J.,Pathology Queensland | Pretorius C.J.,University of Queensland
Clinical Chemistry and Laboratory Medicine | Year: 2016

The reliable detection of paraprotein in serum and urine is the primary purpose of electrophoretic procedures in clinical laboratories. Screening immunofixation electrophoresis (sIFE) employs a single application of antisera directed against heavy and light chains that facilitates the detection of paraproteins that migrate in the non-γ region or that are below the detection limit of protein electrophoresis. These paraproteins that are missed by routine electrophoresis occur in up to 27.3% of newly investigated and 13.6% of monitored patients. Small paraproteins missed by conventional electrophoretic techniques are clinically important in the diagnosis and monitoring of malignant plasma and B-cell disorders. The superior diagnostic performance of sIFE makes it suitable as the initial laboratory procedure to investigate paraproteins in complex serum and urine matrices. © 2016 by De Gruyter 2016. Source


Chia K.M.,University of Queensland | Liu J.,University of Queensland | Francis G.D.,Pathology Queensland | Naderi A.,University of Queensland
Neoplasia | Year: 2011

Estrogen receptor (ER)-negative breast cancer is heterogeneous, and the biology of this disease has remained poorly understood. Molecular apocrine is a subtype of ER-negative breast cancer that is characterized by the overexpression of steroid-response genes such as AR and a high rate of ErbB2 amplification. In this study, we have identified a positive feedback loop between the AR and extracellular signal-regulated kinase (ERK) signaling pathways in molecular apocrine breast cancer. In this process, AR regulates ERK phosphorylation and kinase activity. In addition, AR inhibition results in the down-regulation of ERK target proteins phospho-RSK1, phospho-Elk-1, and c-Fos using an in vivo molecular apocrine model. Furthermore, we show that AR-mediated induction of ERK requires ErbB2, and AR activity, in turn, regulates ErbB2 expression as an AR target gene. These findings suggest that ErbB2 is an upstream connector between the AR and ERK signaling pathways. Another feature of this feedback loop is an ERK-mediated regulation of AR. In this respect, the inhibition of ERK phosphorylation reduces AR expression and CREB1-mediated transcriptional regulation of AR acts as a downstream connector between the AR and ERK signaling pathways inmolecular apocrine cells. Finally, we demonstrate that AR-positive staining is associated with the overexpression of ERK signaling targets phospho-Elk-1 and c-Fos in ER-negative breast tumors, which further supports a cross-regulation between the AR and ERK signaling pathways in molecular apocrine subtype. This study demonstrates an AR-ERK feedback loop in ER-negative breast cancer with significant biologic and therapeutic implications in this disease. © 2011 Neoplasia Press, Inc. All rights reserved. Source

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