Pasteur St Petersburg Research Institute Of Epidemiology And Microbiology

Saint Petersburg, Russia

Pasteur St Petersburg Research Institute Of Epidemiology And Microbiology

Saint Petersburg, Russia

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Elezov D.S.,Pasteur St Petersburg Research Institute Of Epidemiology And Microbiology | Kudryavtsev I.V.,Saint Petersburg State University | Arsent'ev N.A.,Pasteur St Petersburg Research Institute Of Epidemiology And Microbiology | Basin V.V.,Saint Petersburg State University | And 3 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2015

Flow cytometry was employed to examine the content of major populations of memory T helper cells and expression of chemokine receptors CXCR3 and CCR6 on their surface in peripheral blood drawn from virtually healthy people and the patients with chronic viral hepatitis C. The following combination of monoclonal antibodies had been used: CD62LFITC/CD45RA-PE/CD3-ECD/CCR6-PC7/CXCR3-APC/CD4-APC-Cy7. In comparison with control group, the patients with chronic hepatitis C had a smaller number of populations of naïve CD4+ T cells and central memory CD4+ T cells but a greater number of terminally differentiated effector memory CD4+ T cells and effector memory CD4+ T cells. No differences were revealed between CD4+ T cell populations of both groups in expression of CXCR3 and CCR6 receptors. © 2015, Springer Science+Business Media New York.


Arsent'eva N.A.,Pasteur St Petersburg Research Institute Of Epidemiology And Microbiology | Semenov A.V.,St. Petersburg State Medical University | Lyubimova N.E.,Pasteur St Petersburg Research Institute Of Epidemiology And Microbiology | Ostankov Y.V.,Pasteur St Petersburg Research Institute Of Epidemiology And Microbiology | And 7 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2015

We performed a comprehensive analysis of CCR6 and CXCR3 chemokine receptors and their ligands CCL20/MIP-3α, CXCL9/MIG, CXCL10/IP-10, and CXCL11/ITAC in the liver and blood of patients with chronic hepatitis C at different stages of the disease. TaqMan PCR was used to determine mRNA gene expression of chemokines and their receptors in liver specimens, xMAP multiplex analysis was performed to estimate the concentration of chemokines in blood plasma, and fl ow cytofluorometry was used to evaluate CCR6 and CXCR3 expression on peripheral blood lymphocyte populations. In the liver of patients with hepatitis C, mRNA expression of CXCL10, CCR6, and CXCR3 genes increases with fibrosis progression in the liver tissue. In the plasma, concentrations of all studied chemokines increased depending on the stage of liver fibrosis, CCR6 and CXCR3 expression was changed in various lymphocyte populations. Thus, chemokines are involved in the immunopathogenesis and fibrogenesis in chronic viral hepatitis C. The results suggest using these chemokines in the diagnosis and prognosis of the disease. © 2015, Springer Science+Business Media New York.


PubMed | Saint Petersburg State University, Pasteur St Petersburg Research Institute Of Epidemiology And Microbiology and Russian Academy of Medical Sciences
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2015

Flow cytometry was employed to examine the content of major populations of memory T helper cells and expression of chemokine receptors CXCR3 and CCR6 on their surface in peripheral blood drawn from virtually healthy people and the patients with chronic viral hepatitis C. The following combination of monoclonal antibodies had been used: CD62LFITC/CD45RA-PE/CD3-ECD/CCR6-PC7/CXCR3-APC/CD4-APC-Cy7. In comparison with control group, the patients with chronic hepatitis C had a smaller number of populations of nave CD4(+) T cells and central memory CD4(+) T cells but a greater number of terminally differentiated effector memory CD4(+) T cells and effector memory CD4(+) T cells. No differences were revealed between CD4(+) T cell populations of both groups in expression of CXCR3 and CCR6 receptors.


PubMed | Saint Petersburg State University, Pasteur St Petersburg Research Institute Of Epidemiology And Microbiology, Russian Academy of Medical Sciences and S M Kirov Military Medical Academy
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2015

We performed a comprehensive analysis of CCR6 and CXCR3 chemokine receptors and their ligands CCL20/MIP-3, CXCL9/MIG, CXCL10/IP-10, and CXCL11/ITAC in the liver and blood of patients with chronic hepatitis C at different stages of the disease. TaqMan PCR was used to determine mRNA gene expression of chemokines and their receptors in liver specimens, xMAP multiplex analysis was performed to estimate the concentration of chemokines in blood plasma, and fl ow cytofluorometry was used to evaluate CCR6 and CXCR3 expression on peripheral blood lymphocyte populations. In the liver of patients with hepatitis C, mRNA expression of CXCL10, CCR6, and CXCR3 genes increases with fibrosis progression in the liver tissue. In the plasma, concentrations of all studied chemokines increased depending on the stage of liver fibrosis, CCR6 and CXCR3 expression was changed in various lymphocyte populations. Thus, chemokines are involved in the immunopathogenesis and fibrogenesis in chronic viral hepatitis C. The results suggest using these chemokines in the diagnosis and prognosis of the disease.

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