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Mehravar M.,Pasteur Institute of Iran and 69 Pasteur Ave | Mehravar M.,Shahed University | Sardaria S.,Pasteur Institute of Iran and 69 Pasteur Ave | Owliab P.,Shahed University
Indian Journal of Experimental Biology | Year: 2011

Increased resistance of pathogens toward existing antibiotics has compelled the research efforts to introduce new antimicrobial substances. Drugs with new and less resistant-prone targets to antimicrobial activity have a high priority for drug development activities. Cell membrane seems to be a potential target for new antibiotic agent development to overcome resistance. In this study, A total number of 67 actinomycetes were isolated from the soil samples collected from desert, farming and mineral parts of Iran. We used a chromatic sensor as a membrane model that was set up for the target of antimicrobial metabolites of actinomycetes isolated from the soil. The sensors particles were composed of phospholipid and polymerized polydiacetylene (PDA) lipids. These polymers exhibited color change following interaction with membraneactive metabolites. The color change was due to structural disorder in the lipids following their interaction with membraneactive metabolites. The resultant color change was recorded by fluorescent microscope and easily recognizable by naked eye as well. Sixteen strains were isolated which produced antimicrobial metabolites and were effective against test microorganisms (Escherichia coli, Candida albicans and Saccharomyces cerevisiae). A total number of 3 out of 16 strains produced membrane-active metabolites. These 3 strains were identified using 16s rRNA as Streptomyces sp and submitted to GenBank (accession no. JN180853; JN180854; JN180855).

Ramazani A.,Pasteur Institute of Iran and 69 Pasteur Ave | Ramazani A.,Zanjan University of Medical Sciences | Sardari S.,Pasteur Institute of Iran and 69 Pasteur Ave | Zakeri S.,Pasteur Institute of Iran and 69 Pasteur Ave | Vaziri B.,Pasteur Institute of Iran and 69 Pasteur Ave
Parasitology Research | Year: 2010

The extract from Artemisia annua, containing artemisinin, has been proven active against multidrug resistant Plasmodium falciparum in previous studies. The purpose of this paper was to study five Artemisia species from Iran for their in vitro and in vivo antimalarial property and detection of artemisinin in the active species by chromatographic and spectroscopic methods including nuclear magnetic resonance (NMR) spectroscopy. Dried plants were extracted by 80% ethanol, and total extracts were investigated for antiplasmodial property and artemisinin content by TLC, HPLC, and 1H-NMR techniques. Two plants (A. annua L. and Artemisia absinthium L.) showed good antiplasmodial activity against multidrug resistant and sensitive strain of P. falciparum. A. absinthium and A. annua at concentrations of 200 mg/kg for 4 days reduced parasitemia in BALB/C mice infected with Plasmodium bergei by 94.28% and 83.28%, respectively, but we could not detect artemisinin in all plants studied in this research. The antiplasmodial property of these two herbs is possibly related to essential oils that present in high amounts in their extracts. © 2010 Springer-Verlag.

Mahmoudi M.,Pasteur Institute of Iran and 69 Pasteur Ave | Shokrgozar M.A.,Pasteur Institute of Iran and 69 Pasteur Ave | Sardari S.,Pasteur Institute of Iran and 69 Pasteur Ave | Moghadam M.K.,Pasteur Institute of Iran and 69 Pasteur Ave | And 3 more authors.
Nanoscale | Year: 2011

The understanding of the interactions between nanomaterials and proteins is of extreme importance in medicine. In a biological fluid, proteins can adsorb and associate with nanoparticles, which can have significant impact on the biological behavior of the proteins and the nanoparticles. We report here on the interactions of iron saturated human transferrin protein with both bare and polyvinyl alcohol coated superparamagnetic iron oxide nanoparticles (SPIONs). The exposure of human transferrin to SPIONs results in the release of iron, which changes the main function of the protein, which is the transport of iron among cells. After removal of the magnetic nanoparticles, the original protein conformation is not recovered, indicating irreversible changes in transferrin conformation: from a compact to an open structure. © 2011 The Royal Society of Chemistry.

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