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Tahghighi A.,Pasteur Institute of Iran
Journal of Organometallic Chemistry | Year: 2014

The occurrence of adverse effects and the development of resistance associated with the administration of conventional anti-leishmanial drugs explicate the urgent need for development of new leishmanicidal agents. The potential use of metal complexes as drugs against parasitic diseases has so far been very little explored. Leishmaniasis affects millions of people around the world with very limited therapeutic options for their treatment. This review focuses on the recent advances in the development of complex anti-leishmania agents. © 2014 Elsevier B.V. All rights reserved.

Bolhassani A.,Pasteur Institute of Iran
Biochimica et biophysica acta | Year: 2011

Cell penetrating peptides (CPPs) are short amphipathic and cationic peptides that are rapidly internalized across cell membranes. They can be used to deliver molecular cargo, such as imaging agents (fluorescent dyes and quantum dots), drugs, liposomes, peptide/protein, oligonucleotide/DNA/RNA, nanoparticles and bacteriophage into cells. The utilized CPP, attached cargo, concentration and cell type, all significantly affect the mechanism of internalization. The mechanism of cellular uptake and subsequent processing still remains controversial. It is now clear that CPP can mediate intracellular delivery via both endocytic and non-endocytic pathways. In addition, the orientation of the peptide and cargo and the type of linkage are likely important. In gene therapy, the designed cationic peptides must be able to 1) tightly condense DNA into small, compact particles; 2) target the condensate to specific cell surface receptors; 3) induce endosomal escape; and 4) target the DNA cargo to the nucleus for gene expression. The other studies have demonstrated that these small peptides can be conjugated to tumor homing peptides in order to achieve tumor-targeted delivery in vivo. On the other hand, one of the major aims in molecular cancer research is the development of new therapeutic strategies and compounds that target directly the genetic and biochemical agents of malignant transformation. For example, cell penetrating peptide aptamers might disrupt protein-protein interactions crucial for cancer cell growth or survival. In this review, we discuss potential functions of CPPs especially for drug and gene delivery in cancer and indicate their powerful promise for clinical efficacy. Copyright © 2011 Elsevier B.V. All rights reserved.

Mahmoudi M.,Ecole Polytechnique Federale de Lausanne | Mahmoudi M.,Pasteur Institute of Iran | Mahmoudi M.,Tehran University of Medical Sciences | Hofmann H.,Ecole Polytechnique Federale de Lausanne | And 3 more authors.
Chemical Reviews | Year: 2012

A study was conducted to assess the in vitro and in vivo toxicity of superparamagnetic iron oxide nanoparticles(SPION). It was observed that the obtained toxicity data varied significantly depending on size, size distribution, surface, and subsequent surface derivatization. It was essential to establish adequate and reproducible analytical environments in terms of the choice of cells, growing conditions, or sample preparation assay procedures to obtain reliable and reproducible data from such in vitro tests. Determination of the possible toxicity of SPIONs was initially determined using in vitro toxicity tests as described for other nanoparticles. Transmission electron microscopy (TEM) and toxicity assays helped researchers in finding that murine macrophage cells exposed to bare SPIONs showed cytoxicities nearing 90% of the asbestos-treated cultures.

The embodiments herein discloses a vaccine against urinary tract infection (UTI). The flagellin (FliC) of enteroaggregative

The present invention discloses the thrombolytic therapy by t-PA or CT-b for the treatment of the acute myocardial infarction. A chimeric truncated form of t-PA or CT-b is designed and expressed in

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