Pasteur Institute of India

Nilgiris, India

Pasteur Institute of India

Nilgiris, India

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Saravanakumar A.,Anna University | Ganesh M.,Anna University | Jayaprakash J.,Pasteur Institute of India | Ramaswamy N.M.,Anna University
International Journal of Phytomedicine | Year: 2010

Hyperlipidemia is the greatest risk factor of coronary heart disease. Currently available hypolipidemic drugs have been associated with number of side effects. Herbal treatment for hyperlipidemia has no side effects and is relatively cheap and locally available. A literature claims that flavonoids can able to reduce the hyperlipidemia. Based on high flavonoid content in herbal, Sesbania grandiflora (SG) was selected and the present study focus on the anti-hyperlipidemic activity of aqueous extract of leaves of SG against triton induced hyperlipidemia in rats. SG administered a dose of 200μg/kg (p.o) to the triton induced hyperlipidemic rats. SG shows a significant decrease in the levels of serum cholesterol, phospholipid, triglyceride, LDL, VLDL and significant increase in the level of serum HDL at the dose of 200μg/kg (p.o) against Triton induced hyperlipidemic in rats. Aqueous extract of leaves of SG was investigated hypolipidemic activity on Triton induced hyperlipidemic profile. Aqueous extract fraction decreased serum level of total cholesterol by 69.72. On the other hand, aqueous extract of SG increased the serum HDL cholesterol level by 24.11. The reduction of LDL cholesterol level by aqueous extract was 30.31. ©arjournals.org, All rights reserved.


Bhuyan P.J.,National Center for Disease Control | Nath A.J.,Pasteur Institute of India
Journal of Arthropod-Borne Diseases | Year: 2016

Background: Tropical rat mite (Ornithonyssus bacoti) is reported from many parts of the world and is considered important in transmitting rickettsial pathogens. There have been scanty reports on prevalence of this parasite from India. Following a recent report of O. bacoti infestation in a laboratory mice colony from Nilgiris, Tamil Nadu, India, attempts were made to detect the parasite in its natural reservoir, ie the domestic and peridomestic rats (Rattus rattus). Methods: The National Centre for Disease Control, Coonoor is involved in screening plague in domestic and peridomestic rats in Nilgiris and erstwhile plague endemic areas of Southern India. The parasite samples were identified based on the morphological characteristics attributable to O. bacoti and as per description of published literature. Results: Seven mite samples identified as O. bacoti based on morphological characteristics were isolated incidentally from domestic and peridomestic rodents in and around the hilly districts of Nilgiris, Southern India, during the routine plague surveillance programme. The identification was based on the morphological characteristics attributable to O. bacoti observed under a low power microscope. Conclusion: In India, this is probably the first record of isolation of O. bacoti from domestic and peridomestic rodents. Prevalence of such parasite in domestic and peridomestic rats necessitates further investigation on monitoring and surveillance of rickettsial diseases in the locality, as these parasites are considered to be potential vector of transmitting rickettsial pathogens.


Amudhan S.M.,Sri Ramachandra University | Sekar U.,Sri Ramachandra University | Arunagiri K.,Central Leprosy Teaching and Research Institute | Sekar B.,Pasteur Institute of India
Indian Journal of Medical Microbiology | Year: 2011

Objectives: Acinetobacter baumannii is a significant pathogen in health care settings. In recent years, an increase in carbapenem resistance among A. baumannii due to Ambler class B metallo-betaβ-lactamases or class D OXA carbapenamases has been reported. In this study we detected the presence of OXA carbapenamases and coproduction of metallo-betaβ-lactamases (bla VIM and blaIMP ) by phenotypic and genotypic methods in carbapenem resistant clinical isolates of Acinetobacter baumannii. Materials and Methods: A total of 116 consecutive, non-duplicate carbapenem resistant A. baumannii isolated from various clinical specimens were included in the study. The modified Hodge test and inhibitor potentiated disk diffusion tests were done for the screening of carbapenamase and metallo-betaβ-lactamase production, respectively. Polymerase chain reaction (PCR) was performed for the detection of OXA (blaOXA 23 like, blaOXA 24 like, bla OXA-51 like and blaOXA-58 like genes) and metallo-betaβ-lactamases (blaVIM and blaIMP ) genes. Gene sequencing was performed for representative isolates. Results: Among 116 A. baumannii, OXA genes were detected in 106 isolates. BlaOXA 51 like (n = 99) and blaOXA -23 like (n = 95) were the most common and they coexisted in 89 isolates. blaOXA-24 like gene was detected in two isolates of which one also carried blaOXA-51 like and bla OXA-58 like genes. The modified Hodge test was positive in 113 isolates. The metallo-betaβ-lactamase screening test was positive in 92 isolates. blavim was detected in 54 isolates of which 1 also carried the blaIMP gene. Conclusions: blaOXA-23 like and bla OXA 51 like genes are the most common types of OXA carbapenamases while the blaVIM type is the most common type of metallo-betaβ- lactamase contributing to carbapenem resistance in clinical isolates of A. baumannii. The coproduction of OXA and metallo-betaβ-lactamases is not an uncommon phenomenon in A. baumannii.


Shanthi M.,Sri Ramachandra University | Sekar U.,Sri Ramachandra University | Kamalanathan A.,Central Leprosy Teaching and Research Institute | Sekar B.,Central Leprosy Teaching and Research Institute | Sekar B.,Pasteur Institute of India
Indian Journal of Medical Research | Year: 2014

Background & objectives: New Delhi metallo β-lactamase-1 (NDM-1) producing Pseudomonas aeruginosa isolates are potential threat to human health. This study was conducted to detect the presence of blaNDM-1 in carbapenem resistant P. aeruginosa in a tertiary care center in southern India.Methods: Sixty one carbapenem resistant clinical isolates of a total of 212 P. aeruginosa isolates cultured during the study period were screened for the presence of NDM-1by PCR. Clinical characteristics of the NDM-1 positive isolates were studied and outcome of the patients was followed up.Results: Of the 61 isolates, NDM-1 was detected in four isolates only. These were isolated from patients in the intensive care units and chest medicine ward. The source specimens were pus, sputum, bronchoalveolar lavage and endotracheal aspirate. The NDM-1 producers were susceptible only to polymyxin B. Only one patient responded to polymyxin B therapy, while the others succumbed to the infection.Conclusion: These findings reveal that NDM-1 is not a major mechanism mediating carbapenem resistance in P. aeruginosa in this centre. However, continuous surveillance and screening are necessary to prevent their dissemination. © 2014, Indian Council of Medical Research. All rights reserved.


Barman N.N.,Assam Agricultural University | Nath A.J.,Pasteur Institute of India | Neog B.K.,Assam Agricultural University | Das G.,Assam Agricultural University
VirusDisease | Year: 2014

Rotavirus is prevalent worldwide and has been established as a leading cause of mortality due to severe diarrhoea in neonates. Isolation of the virus is a gold standard method for confirmation of rotavirus infection in the host. Propagation of rotavirus in cell culture is a challenge as in many instances the virus does not produce detectable cytopathic effect. This study was undertaken to evaluate the efficacy of fluorescent antibody test (FAT), immunoperoxidase test (IPT) and sandwich ELISA (S-ELISA) to detect rotavirus antigen propagated in MA 104 cell line. The intensity of fluorescence and colour development for I-FAT and I-IPT was categorized and the ELISA OD values were analyzed. The overall mean of detection were 5.16 ± 0.47, 5.16 ± 0.54 and 5.66 ± 0.33 for I-FAT, I-IPT and S-ELISA, respectively. Significantly less number of samples were positive in the initial one or two passage, which increased up to 100 % from third/fourth passage onwards. The study concluded that I-FAT, I-IPT and S-ELISA were equally effective in detecting propagated rotavirus in cell line, and the former two tests are suitable for in situ demonstration of the virus while the later could be used to assay antigen in cell culture fluid. © 2014, Indian Virological Society.


Jagannathan S.,Pasteur Institute of India | Gandhi P.R.,Jamal Mohammed College | Vijayakumar R.,Karpagam Arts and Science College
Journal of Biological Sciences | Year: 2013

Rabies is an acute disease of vertebrate animals which may affect all animals from amphibian to man and it is a single stranded negative sense RNA virus belonging to the genus Lyssavirus of the family Rhabdoviridae. The production of vaccines, diagnosis and further research will therefore continue in this field because a cure for rabies would be a big boon for the developing countries. Virus inactivation is essential for the preparation of vaccines, diagnostic reagent and research purposes. The beta propiolactone (βPL) is widely used as an inactivatent; βPL is very considerable value in the production of biological for virus inactivation and perhaps for DNA inactivation. The inactivation results indicate that the rate of inactivation is directly proportional to the concentration of βPL used. Assessment of the residual βPL present in the vaccine is a mandatory test to vaccine. Vaccine manufacturing also assess the efficacy of inactivation procedure by various in process quality control tests such as tissue culture virus amplification test and inactivation kinetics. This experiments are based on the changing the parameters as well as the various concentration of βPL in the clarified, concentrated high titer of P VI1 rabies strain, subsequently that are further purified and formulated with suitable additives and stabilizers. The immunogenicity of the vaccines is analyzed. During the βPL inactivation kinetics gives an idea of the predictable time of inactivation and also the order of inactivation, validation of the inactivation process is an essential part of quality assurance. In the immunobiological industry the time is a crucial factor because to get more inactivated viral harvest yield in a short period and they are processed to further purification and downstream processing. This findings is to reduce the time frame and the residual βPL in various level influenced the tissue culture derived anti rabies vaccine. © 2013 Asian Network for Scientific Information.


Sivananda N.,Pasteur Institute of India
Nature Environment and Pollution Technology | Year: 2010

The effluents collected from Sago industry before and after treatment were analysed for the physicochemical characteristics. The effect of treated sago industry effluent in different concentrations (10%, 25%, 50% and 100%) on seed germination and biochemical changes during germination of paddy were studied and compared with the control. In the treated effluent pH, TSS, TDS, BOD and COD were found to be within the tolerance limit and it can be used for irrigation purposes. The seed germination percentage declined with the increasing concentration of the treated effluent. Generally, in paddy the protein, starch and chlorophyll contents were increased from 6th day to 12 th day and then declined. It was observed that when the treated effluent is diluted particularly at 10 %, it influences the biochemical parameters and at the same time the increased concentration of the effluent reduces the protein, starch and chlorophyll contents in experimental leaves when compared to control.


PubMed | Pasteur Institute of India
Type: | Journal: Biologicals : journal of the International Association of Biological Standardization | Year: 2016

The protective effect of therapeutic immunization with heat inactivated Helicobacter pylori cells administered with aluminum phosphate as an adjuvant was evaluated with Swiss albino mice infected with H.pylori Sydney strain 1 (SS1). The presence of bacteria in histological sections of the stomach was evaluated to confirm the colonization of H.pylori. The infection dose was determined to be 110


PubMed | Assam Agricultural University and Pasteur Institute of India
Type: Journal Article | Journal: Virusdisease | Year: 2015

Rotavirus is prevalent worldwide and has been established as a leading cause of mortality due to severe diarrhoea in neonates. Isolation of the virus is a gold standard method for confirmation of rotavirus infection in the host. Propagation of rotavirus in cell culture is a challenge as in many instances the virus does not produce detectable cytopathic effect. This study was undertaken to evaluate the efficacy of fluorescent antibody test (FAT), immunoperoxidase test (IPT) and sandwich ELISA (S-ELISA) to detect rotavirus antigen propagated in MA 104 cell line. The intensity of fluorescence and colour development for I-FAT and I-IPT was categorized and the ELISA OD values were analyzed. The overall mean of detection were 5.160.47, 5.160.54 and 5.660.33 for I-FAT, I-IPT and S-ELISA, respectively. Significantly less number of samples were positive in the initial one or two passage, which increased up to 100% from third/fourth passage onwards. The study concluded that I-FAT, I-IPT and S-ELISA were equally effective in detecting propagated rotavirus in cell line, and the former two tests are suitable for in situ demonstration of the virus while the later could be used to assay antigen in cell culture fluid.


PubMed | Pasteur Institute of India
Type: Journal Article | Journal: Journal of parasitic diseases : official organ of the Indian Society for Parasitology | Year: 2016

A case of sarcoptic mange caused by

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