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Nilgiris, India

Jagannathan S.,Pasteur Institute of India | Mani K.R.,Pasteur Institute of India | Vijayakumar R.,Karpagam Arts and Science College | Rahul Gandhi P.,Jamal Mohammed College | Venkataramana K.N.,Pasteur Institute of India
International Journal of Pharma and Bio Sciences | Year: 2011

Rabies is normally a rapidly fatal neurological disease and to date therapeutic efforts in humans have proved futile except in rare cases in which rabies vaccine was administered prior to the onset of clinical disease. Safe and potent vaccines produced by cell culture techniques are available in developed countries. One of the most elements in the effective control of human rabies is the use of efficacious vaccines. The stabilizers play an important role in the efficacy of vaccines. Our present study highlights the analysis of Magnesium Chloride as a stabilizer in the newly developed vero cell derived rabies vaccine. Source

Jagannathan S.,Pasteur Institute of India | Gandhi P.R.,Jamal Mohammed College | Vijayakumar R.,Karpagam Arts and Science College
Journal of Biological Sciences | Year: 2013

Rabies is an acute disease of vertebrate animals which may affect all animals from amphibian to man and it is a single stranded negative sense RNA virus belonging to the genus Lyssavirus of the family Rhabdoviridae. The production of vaccines, diagnosis and further research will therefore continue in this field because a cure for rabies would be a big boon for the developing countries. Virus inactivation is essential for the preparation of vaccines, diagnostic reagent and research purposes. The beta propiolactone (βPL) is widely used as an inactivatent; βPL is very considerable value in the production of biological for virus inactivation and perhaps for DNA inactivation. The inactivation results indicate that the rate of inactivation is directly proportional to the concentration of βPL used. Assessment of the residual βPL present in the vaccine is a mandatory test to vaccine. Vaccine manufacturing also assess the efficacy of inactivation procedure by various in process quality control tests such as tissue culture virus amplification test and inactivation kinetics. This experiments are based on the changing the parameters as well as the various concentration of βPL in the clarified, concentrated high titer of P VI1 rabies strain, subsequently that are further purified and formulated with suitable additives and stabilizers. The immunogenicity of the vaccines is analyzed. During the βPL inactivation kinetics gives an idea of the predictable time of inactivation and also the order of inactivation, validation of the inactivation process is an essential part of quality assurance. In the immunobiological industry the time is a crucial factor because to get more inactivated viral harvest yield in a short period and they are processed to further purification and downstream processing. This findings is to reduce the time frame and the residual βPL in various level influenced the tissue culture derived anti rabies vaccine. © 2013 Asian Network for Scientific Information. Source

Amudhan S.M.,Sri Ramachandra University | Sekar U.,Sri Ramachandra University | Arunagiri K.,Central Leprosy Teaching and Research Institute | Sekar B.,Pasteur Institute of India
Indian Journal of Medical Microbiology | Year: 2011

Objectives: Acinetobacter baumannii is a significant pathogen in health care settings. In recent years, an increase in carbapenem resistance among A. baumannii due to Ambler class B metallo-betaβ-lactamases or class D OXA carbapenamases has been reported. In this study we detected the presence of OXA carbapenamases and coproduction of metallo-betaβ-lactamases (bla VIM and blaIMP ) by phenotypic and genotypic methods in carbapenem resistant clinical isolates of Acinetobacter baumannii. Materials and Methods: A total of 116 consecutive, non-duplicate carbapenem resistant A. baumannii isolated from various clinical specimens were included in the study. The modified Hodge test and inhibitor potentiated disk diffusion tests were done for the screening of carbapenamase and metallo-betaβ-lactamase production, respectively. Polymerase chain reaction (PCR) was performed for the detection of OXA (blaOXA 23 like, blaOXA 24 like, bla OXA-51 like and blaOXA-58 like genes) and metallo-betaβ-lactamases (blaVIM and blaIMP ) genes. Gene sequencing was performed for representative isolates. Results: Among 116 A. baumannii, OXA genes were detected in 106 isolates. BlaOXA 51 like (n = 99) and blaOXA -23 like (n = 95) were the most common and they coexisted in 89 isolates. blaOXA-24 like gene was detected in two isolates of which one also carried blaOXA-51 like and bla OXA-58 like genes. The modified Hodge test was positive in 113 isolates. The metallo-betaβ-lactamase screening test was positive in 92 isolates. blavim was detected in 54 isolates of which 1 also carried the blaIMP gene. Conclusions: blaOXA-23 like and bla OXA 51 like genes are the most common types of OXA carbapenamases while the blaVIM type is the most common type of metallo-betaβ- lactamase contributing to carbapenem resistance in clinical isolates of A. baumannii. The coproduction of OXA and metallo-betaβ-lactamases is not an uncommon phenomenon in A. baumannii. Source

Bhuyan P.J.,National Center for Disease control | Nath A.J.,Pasteur Institute of India
Journal of Arthropod-Borne Diseases | Year: 2016

Background: Tropical rat mite (Ornithonyssus bacoti) is reported from many parts of the world and is considered important in transmitting rickettsial pathogens. There have been scanty reports on prevalence of this parasite from India. Following a recent report of O. bacoti infestation in a laboratory mice colony from Nilgiris, Tamil Nadu, India, attempts were made to detect the parasite in its natural reservoir, ie the domestic and peridomestic rats (Rattus rattus). Methods: The National Centre for Disease Control, Coonoor is involved in screening plague in domestic and peridomestic rats in Nilgiris and erstwhile plague endemic areas of Southern India. The parasite samples were identified based on the morphological characteristics attributable to O. bacoti and as per description of published literature. Results: Seven mite samples identified as O. bacoti based on morphological characteristics were isolated incidentally from domestic and peridomestic rodents in and around the hilly districts of Nilgiris, Southern India, during the routine plague surveillance programme. The identification was based on the morphological characteristics attributable to O. bacoti observed under a low power microscope. Conclusion: In India, this is probably the first record of isolation of O. bacoti from domestic and peridomestic rodents. Prevalence of such parasite in domestic and peridomestic rats necessitates further investigation on monitoring and surveillance of rickettsial diseases in the locality, as these parasites are considered to be potential vector of transmitting rickettsial pathogens. Source

Vijayakumar R.,Karpagam Arts and Science College | Jagannathan S.,Pasteur Institute of India | Chaansha S.,Kongunadu Arts and Science College
Nano Biomedicine and Engineering | Year: 2011

The present era of nanotechnology has reached to a stage where scientist are able to develop and programme complex machines that are built at molecular level which can work inside the patient body. One such challenge is a nanorobot, which once thought to be a desire as came into reality now. The proposed application of nanorobot can range from common cold to dreadful diseases like cancer, Diabetes, influenza, cerebral aneurysm. This study of nanorobot serves as a lead to the field of nanomedicine. There are many applications for nanorobotic systems and its biggest impact would be in the area of medicine. This article deals with the nanorobotic design and their applications in molecular diagnosis. © 2011 Vijayakumar R, et al. Source

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