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Onishchenko G.G.,Government of the Russian Federation | Dyatlov I.A.,State Research Center for Applied Microbiology and Biotechnology | Svetoch E.A.,State Research Center for Applied Microbiology and Biotechnology | Volozhantsev N.V.,State Research Center for Applied Microbiology and Biotechnology | And 21 more authors.
Vestnik Rossiiskoi Akademii Meditsinskikh Nauk | Year: 2015

Shiga toxin-producing Escherichia coli (STEC) food-borne infections are reported worldwide and represent a serious problem for public healthcare. In the Russian Federation there is little information on epidemiology and etiology of STEC-infections as well as on molecular-genetic peculiarities of STEC pathogens. Objective: Our aim was to describe a food-borne outbreak as hemorrhagic colitis (HC) along with hemolytic uremic syndrome (HUS), enterocolitis, and acute gastroenteritis in children in St.-Petersburg in 2013. Methods: Epidemiological, microbiological, molecular-genetic and bioinformati c methods were applied. Results: Objects to study were clinical specimens, milk and food samples, as well as STEC strains isolated during the outbreak. The outbreak of food-borne infection was found to be caused by STEC-contaminated raw milk as confirmed by epidemiological analysis, detection of STEC DNA and isolation of relevant pathogens in milk and sick children fecal specimens. The whole-genome sequencing revealed two groups of pathogens, E. coli O157:H7 and E. coli O101:H33 among collected strains. Group I strains were attributed to the previously known sequence type ST24, while group II strains belonged to the previously non-described sequence type ST145. In strain genomes of both groups there were identified nucleotide sequences of VT2-like prophage carrying stx2c gene, plasmid enterohemolysin gene, and gene of the STEC main adhesion factor intimin. Gene of intimin gamma was identified in E. coli O157:H7 strains and intimin iota 2 in E. coli O101:H33 strains. The latter previously was identified only in enteropathogenic E. coli (EPEC) strains. Conclusion: The additional knowledge of epidemiology and biology of STEC pathogens would assist clinicians and epidemiologists in diagnosing, treating and preventing hemorrhagic colitis. © 2015, Izdatel'stvo Meditsina. All rights reserved.


Kuzubova N.A.,Saint Petersburg State University | Chukhlovin A.B.,Saint Petersburg State University | Morozova E.B.,Saint Petersburg State University | Totolian A.A.,Pasteur Research Institute of Epidemiology and Microbiology | Titova O.N.,Saint Petersburg State University
Respiratory Medicine | Year: 2013

Background Pathogenesis of chronic obstructive lung disease (COPD) includes primary inflammatory events, multiple vascular reactions, remodeling of bronchial and vascular walls. Objective The aim of present single-center study was to assess relations between angiotensin-converting enzyme (ACE) gene and prevalence of clinical symptoms characteristic to COPD. Methods The study involved sixty-three male patients with COPD (44-86 years old, a mean of 60.4 years). COPD diagnostics was performed according to common criteria, including evaluation of systolic pressure in pulmonary artery (SPPA) and endothelial disfunction (ED). Genotyping of ACE I/D was performed by means of gene-specific PCR. Results 1. Allele distribution of studied gene alleles among COPD patients did not differ from control age-matched group. 2. Detectable endothelial dysfunction in COPD patients was shown to correlate with high-producer D allele of ACE gene, at an odds ratio of 6.632 (CI = 1.67-26.31; chi2 = 8.39, p = 0.004). Moreover, detectable ED correlated with numbers of COPD exacerbations per year. Conclusions These findings suggest possible association of the functional ACE D allele with altered vascular responses that may modulate development of distinct COPD symptoms. The results are obtained in a limited clinical cohort, and deserve repeated trials in other groups of COPD patients. © 2012 Elsevier Ltd. All rights reserved.


Romanenkova N.I.,Pasteur Research Institute of Epidemiology and Microbiology | Bichurina M.A.,Pasteur Research Institute of Epidemiology and Microbiology | Rozaeva N.R.,Pasteur Research Institute of Epidemiology and Microbiology
Voprosy Virusologii | Year: 2010

Examination of 281 healthy vaccinees from 6 children's homes could isolate 37 vaccine polioviruses. The detection rate for polioviruses in the children's homes, In which there were children bom to HIV-positive mothers, was significantly higher than that in other children's homes. These children's homes showed the longest poliovirus excretion after vaccination. The RFLP analysis showed that 40% of the strains being examined were intertypic recombinant strains. The sequence analysis demonstrated that about 30% of the strains under study had a small number of nucleotide substitutions In the VP3-VP1 genomic region. The study revealed a correlation between the dynamics of poliovirus excretion after oral vaccination and the kinetics of formation of recombinant poliovirus strains.


Vyazovaya A.A.,Pasteur Research Institute of Epidemiology and Microbiology | Kuyevda D.A.,Central Research Institute of Epidemiology | Trofimova O.B.,Central Research Institute of Epidemiology | Shipulina O.Yu.,Central Research Institute of Epidemiology | And 3 more authors.
Klinichescheskaya Laboratornaya Diagnostika | Year: 2013

The DNA of virus of human papilloma of high carcinogenic risk was detected in 116 cervical samples. At that, the morphological symptoms of background processes are detected in 19 samples, CIN1 in 9, CIN 2 in 23, C1N 3 in 54 (and out of them carcinoma in situ in 13), epidermoid cancer (squamous cell carcinoma) in 11 cases. The viral load of human papilloma of high carcinogenic risk in all samples of DNA exceeded threshold of clinical value (3 lg copies of DNA of human papilloma/105 cells). The genetic typing of human papilloma of high carcinogenic risk revealed the dominance of human papilloma of type 16 in 49.7%, type 33 in 15.3%, type 31 in 12.3% and type 45 in 5.5%. In women with background processes in cervix of the uterus DNA of human papilloma type 16 was detected more often in episome form. In case of dysplastic alterations of epithelium and cervical cancer DNA of human papilloma type 16 is detected in mixt form with different degree of integration into cell genome.


Kudryavtsev I.V.,Far Eastern Federal University | Serebryakova M.K.,National research University of information technologies | Totolyan A.A.,Pasteur Research Institute of Epidemiology and Microbiology
Klinichescheskaya Laboratornaya Diagnostika | Year: 2016

The study was carried out to develop standard indicators of relative and absolute content of main populations ofT-helpers in peripheral blood of conditionally healthy donors. The examination was implemented to sampling of 52 healthy individuals (29 males and 23 females) aged 18-65 years (median is 30 years). The multicolor cytofluorimetric analysis was applied using panel of following antibodies: CD45RA-FITC, CD62L-PE, CCR4-PerCP/Cy5.5; CCR6-PE/Cy7, CXCR3-APC, CD3-APC-AF750, CD4-Pacific Blue and CXCRS-Brilliant Violet 510TM. The T-helpersl were distributed in populations of cells with phenotypes CXCR5-CXCR3+CCR6- CCR4-, also containing Th9, andCXCR5-CXCR3+CCR6+CCR4- referred as Thl/Thl7. The Th2 were detected on the basis of availability of CCR4 at the absence of all other chemokin receptors. The Thi7, besides Thl/Thi7 mentioned above, were detected in composition of CXCR5-CXCR3-CCR6+CCR4- And CXCR5-CXCR3-CCR6+CCR4+. The last population also contained Th22. The follicular Th which expressed at their surface CXCR5, formed six cellular populations withfollowing phenotypes: CXCR5+CXCR3- CCR6-CCR4- (TJh/TJh2), CXCR5+CXCR3-CCR6-CCR4+ (T/h2), CXCR5+CXCR3-CCR6+CCR4- (Tfhl7), CXCR5+CXCR3- CCR6+CCR4+ (Tfhl7), CXCR5+CXCR3+CCR6-CCR4- (Tfhl) and CXCR5+CXCR3+CCR6+CCR4- (Tfhl/Tfhl7). The relative and absolute content ofT-helpers ofmentioned phenotypes was established both within theframework oftotalpopulation CD3+CD4+ of lymphocytes and among "naive " T-helpers (CD45RA-CD62L+), T-helpers of central (CD45RA-CD62L+) and effector (CD45RA- CD62L-) memory and also "terminal-differentiated" CD45RA-positive cells ofeffe4ctor memory with phenotype CD45RA+CD62L-. The study results can be applied as standard indicators under diagnostic ofpathologic conditions of immune system.


Starkova D.A.,Pasteur Research Institute of Epidemiology and Microbiology | Mokrousov I.V.,Pasteur Research Institute of Epidemiology and Microbiology | Vyazovaya A.A.,Pasteur Research Institute of Epidemiology and Microbiology | Zhuravlev V.Y.,Research Institute of Physiopulmonology | And 3 more authors.
Molecular Genetics, Microbiology and Virology | Year: 2014

The nontuberculosis mycobacteria Mycobacterium avium subsp. hominissuis (MAH) are able to cause human mycobacteriosis. In this work, results of the first comprehensive study of the genome polymorphism of the clinical strains of MAH are reported for Russia using a typing scheme of 13 MATR VNTR loci (TR292, TRX3, TR25, TR47, MATR-1, MATR-4, MATR-5, MATR-6, MATR-8, MATR-11, MATR-14, MATR-15, MATR-16) containing tandem nucleotide sites and IS1245 RFLP typing sites. Ninety MAH strains isolated from patients with epidemiologically unconnected lung mycobacteriosis (including HIV-positive ones) were tested in 2008-2011. The heterogeneity of the MAH strains in accordance with 36 profiles of 13 MATR VNTR loci was observed. The majority of the strains (68.8%) were in eight MATR VNTR clusters; the biggest cluster contained 37 strains with the 13-bit numerical profile 2222223145443′. The nucleotide sequence of the MATR-16 (3′) locus contains an extensive deletion (GenBank accession no. KF479191). The MAH strains of the MATR VNTR clusters were found to be heterogeneous by the IS1245 marker. The MATR VNTR typing method by 13 loci is recommended for preliminary differentiation of domestic MAH strains with further analysis of the MATR VNTR clusters using the IS1245 RFLP typing method. © 2014, Allerton Press, Inc.


Yermolenko E.I.,Russian Academy of Medical Sciences | Furaeva V.A.,Russian Academy of Medical Sciences | Isakov V.A.,Pasteur Research Institute of Epidemiology and Microbiology | Yermolenko D.K.,Pasteur Research Institute of Epidemiology and Microbiology | Suvorov A.N.,Russian Academy of Medical Sciences
Voprosy Virusologii | Year: 2010

Light and immunofluorescence microscopies were used to study the cytopathic effect of herpes simplex virus type 1 (HSV-1) grown on the Vero cell cultures in the absence or presence of supernatants of Enterococcus faecium L3, Lactobacillus plantarum 8A-P3, and Escherichia coli M17. The effect of the problotic strains was evaluated estimating the proportion of changed cells and the infective dose of the virus. The supernatants of the cultures of Lactobacillus sp. and Enterococcus sp., unlike those of E. coli, have antiviral activity. Inhibited viral replication was more evident when the supernatants were added until the cultured HSV-1 cells were Infective. An enterococcal supernatant and its obtained peptide extract showed the maximum antiviral activity. This strain may be associated with the production of bacteriocins and bacteriocin-like substances.

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