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Valparai, India

Sowndhararajan K.,Bharathiar University | Marimuthu S.,Parry Agro Industries Ltd | Manian S.,Bharathiar University
Journal of Applied Microbiology | Year: 2013

Aims: The present study was aimed to evaluate the integration of Ochrobactrum anthropi BMO-111 and chemical fungicides (copper oxychloride and hexaconazole) against blister blight disease of tea. Methods and Results: Application of the liquid culture of O. anthropi BMO-111 (36-h-old culture broth) was found to be effective in combined sprays with individual chemical fungicides (copper oxychloride and hexaconazole). Spray application of O. anthropi BMO-111 to tea bushes improved the biochemical parameters such as the levels of chlorophyll, polyphenols, and catechins in the harvestable tea shoots. Moreover, in the microplot and large scale trials, the integrated treatment of every two O. anthropi BMO-111 sprays followed by a single fungicides spray was found to be more efficient than the stand alone O. anthropi BMO-111 or chemicals sprays. Further, pathogenicity study employing Swiss albino mice showed no mortality in the test animals when challenged with O. anthropi BMO-111 through oral, intravenous and intranasal routes. Conclusions: The field trials clearly established that O. anthropi BMO-111 has capability to reduce incidence in integrated management of blister blight disease of tea and safe to use in the field. Significance and Impact of the Study: The results indicate that O. anthropi BMO-111 can be used as an agricultural input in the integrated crop protection systems. © 2013 The Society for Applied Microbiology.

Sowndhararajan K.,Bharathiar University | Marimuthu S.,Parry Agro Industries Ltd | Manian S.,Bharathiar University
Journal of Applied Microbiology | Year: 2013

Aims: The present study was carried out to screen the phylloplane bacteria from tea for antagonism against grey blight caused by Pestalotiopsis theae and blister bight caused by Exobasidium vexans and to further evaluate the efficient isolates for disease control potential under field condition. Methods and Results: A total of 316 morphologically different phylloplane bacteria were isolated. Among the antagonists, the isolates designated as BMO-075, BMO-111 and BMO-147 exhibited maximum inhibitory activity against both the pathogens under in vitro conditions and hence were selected for further evaluation under microplot field trial. Foliar application of 36-h-old culture of BMO-111 (1 × 108 colony-forming units ml-1) significantly reduced the blister blight disease incidence than the other isolates. The culture of BMO-111 as well as its culture filtrate effectively inhibited the mycelial growth of various fungal plant pathogens. The isolate BMO-111 was identified as Ochrobactrum anthropi based on the morphological and 16S rDNA sequence analyses. Conclusions: It could be concluded that the biocontrol agent O. anthropi BMO-111 was effective against blister blight disease of tea. Significance and Impact of the Study: Further study is required to demonstrate the mechanism of its action and formulation for the biocontrol potential against blister blight disease of tea. © 2012 The Society for Applied Microbiology.

Moumita S.,National Institute of Technology Rourkela | Banishree S.,National Institute of Technology Rourkela | Indira D.,National Institute of Technology Rourkela | Ajay D.,National Institute of Technology Rourkela | And 8 more authors.
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2015

Tea curd is a functional food providing the health benefits of tea polyphenols and probiotics of curd. Biogenic amines, natural toxins produced by decarboxylation of amino acids, upon consumption in higher levels results in various pathophysiological condition seven leading to death. This work was aimed to study the effect of refrigerated storage on tea polyphenol contentsin tea curd produced by non-biogenic amine forming LAB. Among the probiotic bacteria isolated from different fermented foods in Rourkela, Odisha, India, two were found to be non-biogenic amine forming strains and were utilized for production of black tea curd (BTC) and green tea curd (GTC).The tea polyphenols showed variable stability during storage. There was decrease in contents of EGCG (72% and 74%) and ECG (76% and 74%) in BTC and GTC respectively after 1 day of refrigerated storage owing to conversion of these complex isomers of tea catechins to simpler forms.

Jayabalan R.,Bharathiar University | Jayabalan R.,Chonbuk National University | Jayabalan R.,Karunya University | Baskaran S.,Irt Perundurai Medical College And Research Center | And 3 more authors.
Journal of Applied Biological Chemistry | Year: 2010

Kombucha tea is sugared black tea fermented with a consortium of bacteria and yeasts which forms tea fungus (Medusomyces gisevii) for 14 days. Kombucha tea is claimed to have various beneficial effects on human health but very less scientific evidences are available in the literature. In the present study, the prophylactic and curative effect of black tea (unfermented black tea) and kombucha black tea (KBT) on aflatoxin B1 induced liver damage was studied in male albino rats by analyzing hepatotoxicity markers (aspartate transaminase, alanine transaminase, alkaline phosphatase, gammaglutamyl transpeptidase), lipid peroxidation, reduced glutathione and antioxidant enzymes (glutathione-S-transferase, glutathione peroxidase, glutathione reductase, catalase and superoxide dismutase). Histopathological analysis of liver tissue was also carried out. Results revealed that kombucha tea is more potent in preventing hepatotoxicity than unfermented black tea. The mechanism of hepatoprotection offered by KBT treatment may involve the facilitation of both antioxidant and detoxification processes in the liver.

Jayabalan R.,Bharathiar University | Jayabalan R.,Chonbuk National University | Chen P.-N.,Chung Shan Medical University | Hsieh Y.-S.,Chung Shan Medical University | And 6 more authors.
Indian Journal of Biotechnology | Year: 2011

In the present study, cytotoxic and anti-invasive properties of solvent fractions of kombucha tea were studied. Kombucha tea was fractionated with chloroform, ethyl acetate and butanol. Three solvent extracts and the final aqueous phase were concentrated and used for anti-cancer study at 25, 50, 75 and 100 μg/mL concentrations. Results revealed that ethyl acetate fraction at a concentration of 100 μg/mL caused cytotoxic effect on 786-O and U2OS cells; reduced the activities of matrix metalloproteinase-2 (MMP-2) and-9 in 786-O cells and MMP-2 activity in A549 cells; and significantly reduced the cell invasion and cell motility in A549, U2OS and 786-O cells. Thus, ethyl acetate fraction was further purified by chromatographic studies and presence of dimethyl 2-(2-hydroxy-2-methoxypropylidene) malonate and vitexin were confirmed through IR, NMR and mass spectroscopic studies, which might be responsible for the observed anticancer property of kombucha tea.

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